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  3. Fluorescent Dye
  4. Rhodamine B

Rhodamine B  (Synonyms: 羅丹明B; Basic Violet 10; Brilliant Pink B; Rhodamine O; Tetraethylrhodamine)

目錄號: HY-Y0016 純度: ≥98.0%
COA 產(chǎn)品使用指南

Rhodamine B 是一種熒光染料，常用于染色紡織品、紙張、肥皂、皮革和藥品。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Rhodamine B Chemical Structure

Rhodamine B Chemical Structure

CAS No. : 81-88-9

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     可免費申領(lǐng)三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥440
In-stock
100 mg ￥400
In-stock
1 g ￥700
In-stock
5 g   詢價  
10 g   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Rhodamine B:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Rhodamine B is a staining fluorescent dye, commonly used for dyeing textiles, paper, soap, leather, and agents.

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
518A2 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay
Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay
[PMID: 28033541]
8505C EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human 8505C cells after 96 hrs by SRB assay
Cytotoxicity against human 8505C cells after 96 hrs by SRB assay
[PMID: 28033541]
A2780 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human A2780 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 33049606]
A2780 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human A2780 cells after 96 hrs by SRB assay
Cytotoxicity against human A2780 cells after 96 hrs by SRB assay
[PMID: 28033541]
A-375 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human A-375 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 33049606]
A549 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human A549 cells after 96 hrs by SRB assay
Cytotoxicity against human A549 cells after 96 hrs by SRB assay
[PMID: 28033541]
FaDu EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human FaDu cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 33049606]
HEK293 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human HEK293 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 33049606]
HT-29 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 33049606]
HT-29 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay
Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay
[PMID: 28033541]
MCF7 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 33049606]
MCF7 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay
Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay
[PMID: 28033541]
NIH3T3 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Cytotoxicity against mouse NIH3T3 cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 33049606]
NIH3T3 EC50
> 30 μM
Compound: Rhodamine B
Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay
Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay
[PMID: 28033541]
體外研究
(In Vitro)

Rhodamine B induces a concentration-dependent reduction of root meristem cells of A. cepa. mitotic activity. Rhodamine B induces various nuclear aberrations in A. cepa. root cells. In the 100 and 200 ppm rhodamine B groups, the frequencies of NBUDs and BN surpass those of the positive control (MMS) group. Rhodamine B-induced changes of H2O2 (a) and MDA (b) level increase in a concentration-dependent manner in A. cepa. roots[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

479.01

Formula

C28H31ClN2O3
CAS 號
性狀

固體

顏色

Dark purple to black

Emission (Em)

610
Excitation (Ex)

546
中文名稱

羅丹明B；玫瑰紅B；蕊香紅B；若丹明B；堿性玫瑰精
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : 125 mg/mL (260.95 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

H2O 中的溶解度 : 100 mg/mL (208.76 mM; 超聲助溶)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.0876 mL 10.4382 mL 20.8764 mL
5 mM 0.4175 mL 2.0876 mL 4.1753 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.34 mM); 澄清溶液

    此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。

以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

  • 方案 一

    請依序添加每種溶劑： PBS

    Solubility: 5 mg/mL (10.44 mM); 澄清溶液; 超聲助溶 (<60°C)

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
該產(chǎn)品水溶性佳，請具體參考實測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
純度 & 產(chǎn)品資料

純度: ≥98.0%

染色示例
參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.0876 mL 10.4382 mL 20.8764 mL 52.1910 mL
5 mM 0.4175 mL 2.0876 mL 4.1753 mL 10.4382 mL
10 mM 0.2088 mL 1.0438 mL 2.0876 mL 5.2191 mL
15 mM 0.1392 mL 0.6959 mL 1.3918 mL 3.4794 mL
20 mM 0.1044 mL 0.5219 mL 1.0438 mL 2.6095 mL
25 mM 0.0835 mL 0.4175 mL 0.8351 mL 2.0876 mL
30 mM 0.0696 mL 0.3479 mL 0.6959 mL 1.7397 mL
40 mM 0.0522 mL 0.2610 mL 0.5219 mL 1.3048 mL
50 mM 0.0418 mL 0.2088 mL 0.4175 mL 1.0438 mL
60 mM 0.0348 mL 0.1740 mL 0.3479 mL 0.8698 mL
80 mM 0.0261 mL 0.1305 mL 0.2610 mL 0.6524 mL
100 mM 0.0209 mL 0.1044 mL 0.2088 mL 0.5219 mL

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Rhodamine B
目錄號：
HY-Y0016
需求量：