Peptide C105Y, a synthetic and cell-penetrating peptide based on the amino acid sequence corresponding to residues 359-374 of α1-antitrypsin, enhances gene expression from DNA nanoparticles^[1].

When human hepatoma cells (HuH7) were incubated with fluorescently labeled C105Y for as little as 3 min, C105Y displays nuclear and cytoplasmic staining with enrichment of fluorescent signal in the nucleus and nucleolus. C105Y peptide is routed to the nucleolus very rapidly in an energy-dependent fashion, whereas membrane translocation and nuclear localization are energy-independent. When tested the involvement of known endocytosis pathways in uptake and trafficking of this peptide, C105Y peptide is internalized by a clathrin- and caveolin-independent pathway, although lipid raft-mediated endocytosis may play a role in peptide intracellular trafficking^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1994.40

C₉₇H₁₄₈N₂₀O₂₃S

247572-63-0

Cys-Ser-Ile-Pro-Pro-Glu-Val-Lys-Phe-Asn-Lys-Pro-Phe-Val-Tyr-Leu-Ile

CSIPPEVKFNKPFVYLI

Room temperature in continental US; may vary elsewhere.

• [1]. Rhee M, et al. Mechanism of uptake of C105Y, a novel cell-penetrating peptide. J Biol Chem. 2006 Jan 13;281(2):1233-40.  [Content Brief]