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  2. Neuronal Signaling
  3. Amyloid-β
  4. β-Amyloid (12-28)

β-Amyloid (12-28)  (Synonyms: Amyloid β-Protein (12-28))

目錄號(hào): HY-P1051
產(chǎn)品使用指南 技術(shù)支持

β-Amyloid (12-28) (Amyloid β-Protein (12-28)) 是 β-淀粉樣蛋白 (β1-42) 的肽片段。 β1-42 是 42 個(gè)氨基酸的蛋白質(zhì)，是老年斑核心的主要成分。β-Amyloid (12-28) 具有聚集特性。β-Amyloid (12-28) 有潛力用于阿爾茨海默氏病的研究。

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Custom Peptide Synthesis

β-Amyloid (12-28) Chemical Structure

β-Amyloid (12-28) Chemical Structure

CAS No. : 107015-83-8

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β-Amyloid (12-28) 的其他形式現(xiàn)貨產(chǎn)品:

Customer Review

Other Forms of β-Amyloid (12-28):

  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

β-Amyloid (12-28) (Amyloid β-Protein (12-28)) is a peptide fragment of β-amyloid protein (β1-42). β1-42, a 42 amino acid protein , is the major component of senile plaque cores. β-Amyloid (12-28) shows aggregation properties. β-Amyloid (12-28) has the potential for Alzheimer’s disease research[1].

IC50 & Target

Amyloid-β[1]
體外研究
(In Vitro)

β-amyloid (12-28) may exert dysregulation cognitive effects by means of defective coordination of potassium channel function in nerve, glia and endothelial cells[1].
β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.
2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.
3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.
4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.
Methods vary depends on the downstream applications.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1955.18

Formula

C89H135N25O25
CAS 號(hào)
Sequence

Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys
Sequence Shortening

VHHQKLVFFAEDVGSNK
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
純度 & 產(chǎn)品資料
參考文獻(xiàn)
Cell Assay
[1]

PC12 pheochromocytoma cell lines are routinely cultured in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum,5% horse serum, 1% glutamine and 1% penicillin/streptomycin. Exponentially growing cellsare plated at 30000 cells per well per 100 μL of fresh medium in 96-well tissue cultures plates. Toxicity assays are carried out by measurementof MTT reduction. Two days after plating PC12 pheochromocytoma cell lines are treated with different concentrations of aged β-amyloid (12-28). After 22 h, MTT (5 mg/mL) wasadded and incubation is continued for a further2 h. Cells are lysed and after 24 h at 37°C,colorimetric determination of MTT reduction is made at 550 nm. Positive controls consist of cells in culture medium. Assay values obtained with these controls are taken as 100% viability[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
β-Amyloid (12-28)
目錄號(hào)：
HY-P1051
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