Ac-DEVD-CHO

目錄號: HY-P1001 純度: 99.55%: FAQs
Data Sheet SDS COA 產品使用指南技術支持

Ac-DEVD-CHO 是一種特異性 Caspase-3 抑制劑，K_i 值為 230 pM。

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Custom Peptide Synthesis

Ac-DEVD-CHO Chemical Structure

CAS No. : 169332-60-9

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規(guī)格	價格	是否有貨
1 mg	￥1400	In-stock
5 mg	￥2200	In-stock
10 mg	￥3500	In-stock
25 mg	現(xiàn)貨	詢價
50 mg	現(xiàn)貨	詢價
100 mg		詢價
200 mg		詢價

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Customer Review

MCE 顧客使用本產品發(fā)表的 33 篇科研文獻

•Adv Mater. 2023 Jun;35(23):e2300548. [Abstract]
•Redox Biol. 2024 Jun 8:74:103225. [Abstract]
•J Immunother Cancer. 2024 Mar 1;12(3):e008054. [Abstract]
•Biomaterials. 2022 Sep 29;290:121832. [Abstract]
•J Control Release. 2021 Apr 16;S0168-3659(21)00179-6. [Abstract]
•Cell Death Dis. 2022 Aug 18;13(8):721. [Abstract]

•J Colloid Interface Sci. 29 September 2021.
•Cancer Lett. 2021 Jul 10;S0304-3835(21)00341-4. [Abstract]
•Appl Mater Today. 2021, 101066.
•Basic Res Cardiol. 2021 Jan 23;116(1):3. [Abstract]
•Cell Prolif. 2019 Sep;52(5):e12663. [Abstract]
•Cell Death Discov. 2024 Mar 8;10(1):122. [Abstract]
•J Agric Food Chem. 2023 Oct 3. [Abstract]
•Diabetes. 2022 Jan 14;db210535. [Abstract]
•J Agric Food Chem. 2019 Oct 23;67(42):11718-11727. [Abstract]
•Apoptosis. 2019 Oct;24(9-10):703-717. [Abstract]
•Apoptosis. 2019 Apr;24(3-4):312-325. [Abstract]
•Int J Mol Med. 2024 Nov;54(5):96. [Abstract]
•Toxicology. 2024 Aug 6:153906. [Abstract]
•Chem Biol Interact. 2021 Oct 13;109704. [Abstract]
•Biochem Pharmacol. 2021 Apr 5;114538. [Abstract]
•Genet Mol Biol. 2023 Dec 18.
•Acta Biochim Biophys Sin (Shanghai). 2022, 54(1).
•Vet Microbiol. 2021, 109177.
•Cell Biol Int. 2020 Apr;44(4):937-946. [Abstract]
•PLoS One. 2019 Dec 30;14(12):e0227278. [Abstract]
•Biochimie. 2019 Dec;167:217-227. [Abstract]
•Biosci Biotechnol Biochem. 2020 May;84(5):989-996. [Abstract]
•Journal of Traditional Chinese Medical Sciences. 2024 Jun 5.
•Research Square Preprint. 2024 Apr 12.
•SSRN. 2023 Nov 8.
•Research Square Preprint. 2023 Oct 23.
•Departamento de Biotecnología - Departament de Biotecnologia. 2020 Sep 7.

: Ac-DEVD-CHO purchased from MCE. Usage Cited in: Apoptosis. 2019 Oct;24(9-10):703-717. [Abstract]; BMDMs were first primed with LPS (500 ng/ml) for 4 h, pre-treated with or without MCC950 (1 μM) and caspase-3 inhibitor Ac-DEVD-CHO (DEVD) for 1 h and followed by stimulation with ATP (5 mM) in the presence or absence of MCC950. The cell lysates are collected and western blotting is performed to assess the expression levels of indicated proteins.

Ac-DEVD-CHO 相關產品

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

生物活性
實驗參考方法
純度 & 產品資料
參考文獻

生物活性

Ac-DEVD-CHO is a specific Caspase-3 inhibitor with a K_i value of 230 pM.

IC₅₀ & Target^[1]

Caspase 3

0.23 nM (Ki)

Caspase-8

0.92 nM (Ki)

Caspase-7

1.6 nM (Ki)

Caspase-10

12 nM (Ki)

Caspase-1

18 nM (Ki)

Caspase-6

31 nM (Ki)

Caspase-9

60 nM (Ki)

Caspase-4

132 nM (Ki)

Caspase-5

205 nM (Ki)

Caspase-2

1710 nM (Ki)

細胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
NT2	EC₅₀	> 100 μM Compound: Ac-DEVD-CHO	Inhibition of camptothecin induced apoptosis in human NT2 cells incubated for 5 hrs in presence of camptothecin by ELISA method Inhibition of camptothecin induced apoptosis in human NT2 cells incubated for 5 hrs in presence of camptothecin by ELISA method	[PMID: 33569941]
THP-1	IC₅₀	7.0 x 10^-5 M Compound: 2a	Amidolytic activity of THP.1 cell lysate against IL-1 beta converting enzyme using C1-Asp-AMC as a substrate Amidolytic activity of THP.1 cell lysate against IL-1 beta converting enzyme using C1-Asp-AMC as a substrate	[PMID: 9873373]

體外研究
(In Vitro)

為了確定 caspase-3 在 SLNT 誘導的細胞凋亡中的作用，使用了 caspase-3 抑制劑 (Ac-DEVD-CHO)。添加Ac-DEVD-CHO顯著阻止SLNT誘導的細胞凋亡 (從32.91±1.21%下降到15.88±1.58%，而NC和Ac-DEVD-CHO組分別為6.45±0.96%、7.77±0.79%)^[2]。
zVAD-fmk (5.32%) 或Ac-DEVD-CHO (7.43%) 預處理的細胞凋亡率在金絲桃素介導的PDT處理后明顯下降^[3]。
值得注意的是，10 μMol/L Ac-DEVD-CHO 部分阻斷了 SIN 誘導的細胞凋亡的作用并減少了凋亡細胞核的數(shù)量。SIN 的這些作用被 caspase-3 抑制劑 Ac-DEVD-CHO 阻斷。陽性對照喜樹堿 (4 μM) 可增加 caspase-3 活性，該活性也被 Ac-DEVD-CHO 阻斷^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內研究
(In Vivo)

與模型組比較，CI 組術后各時間點血清 BUN 濃度均顯著降低，Cr 濃度在術后 6 h 顯著降低，12 h、24 h 恢復至假手術組；血清 TNF-α、IL-6 濃度降低，IL-10 濃度在所有時間點均顯著升高。[TNF-α (ug/L) 6小時：436.2±64.2 vs. 653.6±8.9，12小時：233.4±85.4 vs. 579.7±137.1，24小時：151.0±90.3 vs. 551.0±119.8，IL-6 (ug/L) 6小時：1033.2±345.8 vs. 1 595.3±159.4，12小時：366.3±68.3 vs. 1 330.7±249.8，24小時：241.2±208.4 vs. 815.3±572.7，IL-10 (ug/L) 6小時：33.6±10.4 vs. 26.6±4.5，12小時：37.2±5.0 vs. 24.5±4.3，24小時：38.3±5.5 vs. 18.2±1.6，均P<0.05]；腎細胞凋亡率在各時間點均顯著下降：凋亡率6小時：(13.9±3.2) % vs. (18.3±1.4) %，12小時：(10.5±3.6) % vs. (15.9±3.5) %，24小時：(8.4±1.8) % vs. (12.5±2.1) %^[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

502.47

Formula

C₂₀H₃₀N₄O₁₁

CAS 號

169332-60-9

性狀

固體

顏色

White to off-white

Sequence

N-Acetyl-Asp-Glu-Val-Asp-al

Sequence Shortening

Ac-DEVD-al

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性數(shù)據(jù)

細胞實驗：

H₂O 中的溶解度 : ≥ 50 mg/mL (99.51 mM)

* "≥" means soluble, but saturation unknown.

配制儲備液

濃度溶劑體積質量	1 mg	5 mg	10 mg

1 mM	1.9902 mL	9.9508 mL	19.9017 mL
5 mM	0.3980 mL	1.9902 mL	3.9803 mL
10 mM	0.1990 mL	0.9951 mL	1.9902 mL

查看完整儲備液配制表

* 請根據(jù)產品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C儲存時，請在6個月內使用，-20°C儲存時，請在1個月內使用。

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

摩爾計算器
稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動物實驗：

以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內盡快用完。以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

方案一

請依序添加每種溶劑： PBS
Solubility: 100 mg/mL (199.02 mM); 澄清溶液; 超聲助溶

動物溶解方案計算器

請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

每只動物的給藥體積

μL

動物數(shù)量

只

由于實驗過程有損耗，建議您多配一只動物的量

計算結果

工作液所需濃度 : mg/mL

該產品水溶性佳，請具體參考實測水 / PBS / Saline 中的溶解度數(shù)據(jù)。

您所需的儲備液濃度超過該產品的實測溶解度，如有需要，請與 MCE 中國技術支持聯(lián)系。

純度 & 產品資料

純度: 99.55%

選擇批次：

Data Sheet (642 KB) SDS (393 KB)

COA (292 KB) RP-HPLC (256 KB) MS (68 KB)

產品使用指南 (1538 KB)

參考文獻

[1]. Garcia-Calvo M, et al.nhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem. 1998 Dec 4;273(49):32608-13. [Content Brief]

[2]. Jinglin Wang, et al. A polysaccharide from Lentinus edodes inhibits human colon cancer cell proliferation and suppresses tumor growth in athymic nude mice. Oncotarget. 2017 Jan 3; 8(1): 610-623. [Content Brief]

[3]. Junping Zhang, et al. Hypericin-mediated photodynamic therapy induces apoptosis of myoloma SP2/0 cells depended on caspase activity in vitro. Cancer Cell Int. 2015; 15: 58 [Content Brief]

[4]. Long-gang He, et al. Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation. Acta Pharmacol Sin. 2014 Feb; 35(2): 203-210. [Content Brief]

[5]. Liu LX, et al. The effect of caspase-3 inhibitor on the concentrations of serum inflammatory cytokines in sepsis related acute kidney injury induced by peritoneal cavity infection in mice. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Dec;22(12):736-9. [Content Brief]

Cell Assay ^[4]	OCLs are incubated with RANKL and treated with 0.5 mM SIN with or without the specific caspase-3 inhibitor Ac-DEVD-CHO (10 μM) for 24 h. At the end of the treatment, the cells are washed with PBS and are stained for 15 min with 10 μM Hoechst 33258 dye. Images of the staineing cells are captured with a fluorescent microscope. The differences are evaluated by counting the number of cells with apoptotic nuclear condensation in each well^[4]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[5]	One hundred and two male mice are subjected to cecal ligation and puncture or sham operation. The animals are assigned into three equal groups (n=34) according to random number table: sham group, model group, and caspase-3 inhibitor (CI) group. Thirty minutes before CLP, Ac-DEVD-CHO (4 μg/g) is injected subcutaneously in CI group. The levels of blood urea nitrogen (BUN) and creatinine (Cr) are determined, and the concentrations of tumor necrosis factor-α (TNF-α), interleukins (IL-6 and IL-10) are measured by enzyme linked immunosorbent assay (ELISA), the renal cell apoptosis rate is determined by flow cytometry. The 4-day and 7-day survival rates of three groups of mice are observed^[5]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻	[1]. Garcia-Calvo M, et al.nhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem. 1998 Dec 4;273(49):32608-13. [Content Brief] [2]. Jinglin Wang, et al. A polysaccharide from Lentinus edodes inhibits human colon cancer cell proliferation and suppresses tumor growth in athymic nude mice. Oncotarget. 2017 Jan 3; 8(1): 610-623. [Content Brief] [3]. Junping Zhang, et al. Hypericin-mediated photodynamic therapy induces apoptosis of myoloma SP2/0 cells depended on caspase activity in vitro. Cancer Cell Int. 2015; 15: 58 [Content Brief] [4]. Long-gang He, et al. Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation. Acta Pharmacol Sin. 2014 Feb; 35(2): 203-210. [Content Brief] [5]. Liu LX, et al. The effect of caspase-3 inhibitor on the concentrations of serum inflammatory cytokines in sepsis related acute kidney injury induced by peritoneal cavity infection in mice. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Dec;22(12):736-9. [Content Brief]

Ac-DEVD-CHO 相關分類

完整儲備液配制表

可選溶劑	濃度溶劑體積質量	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.9902 mL	9.9508 mL	19.9017 mL	49.7542 mL
	5 mM	0.3980 mL	1.9902 mL	3.9803 mL	9.9508 mL
	10 mM	0.1990 mL	0.9951 mL	1.9902 mL	4.9754 mL
	15 mM	0.1327 mL	0.6634 mL	1.3268 mL	3.3169 mL
	20 mM	0.0995 mL	0.4975 mL	0.9951 mL	2.4877 mL
	25 mM	0.0796 mL	0.3980 mL	0.7961 mL	1.9902 mL
	30 mM	0.0663 mL	0.3317 mL	0.6634 mL	1.6585 mL
	40 mM	0.0498 mL	0.2488 mL	0.4975 mL	1.2439 mL
	50 mM	0.0398 mL	0.1990 mL	0.3980 mL	0.9951 mL
	60 mM	0.0332 mL	0.1658 mL	0.3317 mL	0.8292 mL
	80 mM	0.0249 mL	0.1244 mL	0.2488 mL	0.6219 mL

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-DEVD-CHO169332-60-9CaspaseInhibitorinhibitorinhibit

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Ac-DEVD-CHO

Customer Review

MCE 顧客使用本產品發(fā)表的 33 篇科研文獻