3,5,7,3′,4′-Pentamethoxyflavone

目錄號: HY-N7690 純度: 99.15%: FAQs
Data Sheet SDS COA 產(chǎn)品使用指南技術(shù)支持

3,5,7,3’,4’-Pentamethoxyflavone 是一種 Ca²⁺ 通道 (Ca²⁺ channel) 抑制劑。3,5,7,3’,4’-Pentamethoxyflavone 可以保護(hù) DNA 免受氧化損傷。3,5,7,3’,4’-Pentamethoxyflavone 可通過鈣動員相關(guān)機(jī)制引起人類海綿體松弛。3,5,7,3’,4’-Pentamethoxyflavone 在中年雄性大鼠中可以促進(jìn)血管 eNOS 和胱硫氨酸-γ-裂解酶 CSE 蛋白的表達(dá)，并調(diào)節(jié)血管功能。3,5,7,3’,4’-Pentamethoxyflavone 可用于糖尿病和心血管疾病的研究。

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3,5,7,3′,4′-Pentamethoxyflavone Chemical Structure

CAS No. : 1247-97-8

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規(guī)格	價格	是否有貨
10 mM * 1 mL in DMSO	￥1229	In-stock
1 mg	￥650	In-stock
5 mg	￥1500	In-stock
10 mg	￥2400	In-stock
50 mg		詢價
100 mg		詢價

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3,5,7,3′,4′-Pentamethoxyflavone 相關(guān)產(chǎn)品

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N-type calcium channel L-type calcium channel P/Q-type calcium channel T-type calcium channel Calcium Channel

生物活性
純度 & 產(chǎn)品資料
參考文獻(xiàn)

生物活性

3,5,7,3’,4’-Pentamethoxyflavone is a Ca²⁺ channel inhibitor. 3,5,7,3’,4’-Pentamethoxyflavone can protect DNA from oxidative damage. 3,5,7,3’,4’-Pentamethoxyflavone can induce relaxation of the human corpus cavernosum through calcium mobilization-related mechanisms. 3,5,7,3’,4’-Pentamethoxyflavone can promote the expression of eNOS and cystathionine gamma lyase CSE proteins in middle-aged male rats and regulate vascular function. 3,5,7,3’,4’-Pentamethoxyflavone can be used in research related to diabetes and cardiovascular diseases^[1]^[2]^[4].

細(xì)胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
2008	IC₅₀	26.35 μM Compound: 10	Inhibition of MRP1 (unknown origin) expressed in human 2008 cells assessed as calcein-AM accumulation preincubated for 30 mins before calcein-AM addition measured up to 90 mins by fluorescence assay Inhibition of MRP1 (unknown origin) expressed in human 2008 cells assessed as calcein-AM accumulation preincubated for 30 mins before calcein-AM addition measured up to 90 mins by fluorescence assay	[PMID: 23851114]
A2780 ADR	IC₅₀	23.4 μM Compound: 10	Inhibition of p-glycoprotein (unknown origin) expressed in human A2780Adr cells assessed as calcein-AM accumulation preincubated for 30 mins before calcein-AM addition measured up to 90 mins by fluorescence assay Inhibition of p-glycoprotein (unknown origin) expressed in human A2780Adr cells assessed as calcein-AM accumulation preincubated for 30 mins before calcein-AM addition measured up to 90 mins by fluorescence assay	[PMID: 23851114]
L929	IC₅₀	> 100 μM Compound: 10	Cytotoxicity against mouse L929 cells after 5 days by MTS assay Cytotoxicity against mouse L929 cells after 5 days by MTS assay	[PMID: 22743241]
MDA-MB-231	IC₅₀	2.042 μM Compound: 30	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34971873]
MDCK-II	IC₅₀	0.822 μM Compound: 10	Inhibition of human BCRP expressed in MDCK2 cells assessed as accumulation of Hoechst 33342 preincubated for 30 mins before Hoechst 33342 addition measured after 120 mins by fluorescence assay Inhibition of human BCRP expressed in MDCK2 cells assessed as accumulation of Hoechst 33342 preincubated for 30 mins before Hoechst 33342 addition measured after 120 mins by fluorescence assay	[PMID: 23851114]
MDCK-II	IC₅₀	1.88 μM Compound: 10	Inhibition of human BCRP expressed in MDCK2 cells assessed as accumulation of pheophorbide-A preincubated for 30 mins before pheophorbide-A addition measured after 120 mins by flow cytometry Inhibition of human BCRP expressed in MDCK2 cells assessed as accumulation of pheophorbide-A preincubated for 30 mins before pheophorbide-A addition measured after 120 mins by flow cytometry	[PMID: 23851114]

體外研究
(In Vitro)

3,5,7,3’,4’-Pentamethoxyflavone (25-100 μg/mL) 可以保護(hù) pUC19 質(zhì)粒 DNA 免受氧化損傷^[1]。
3,5,7,3’,4’-Pentamethoxyflavone (25-100 μg/mL) 可以減少小鼠外周血單核細(xì)胞的氧化 DNA 損傷^[1]。
3,5,7,3’,4’-Pentamethoxyflavone 通過與電壓依賴性 Ca²⁺ 通道和鈣動員相關(guān)機(jī)制引起人類海綿體松弛^[2]。
3,5,7,3’,4’-Pentamethoxyflavone (100 μM, 48 h) 可以增加人腸道 Caco-2 細(xì)胞中腸道緊密連接 (TJ) 蛋白的表達(dá)并發(fā)揮體外腸道屏障促進(jìn)作用^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[3].

Cell Line:	Human intestinal Caco-2 cells
Concentration:	100 μM
Incubation Time:	48 h
Result:	Increased the cytoskeletal association of TJ proteins, such as ZO1, ZO2, occludin,claudin-1, claudin-3, and claudin-4, in the cells.

體內(nèi)研究
(In Vivo)

3,5,7,3’,4’-Pentamethoxyflavone (22 mg/kg; 每天 2 次; 6 周; 口服) 在中年雄性大鼠中可以促進(jìn)血管 eNOS 和胱硫氨酸-γ-裂解酶 CSE 蛋白的表達(dá)，并通過對抗苯腎上腺素的收縮和增強(qiáng)乙酰膽堿的血管舒張來調(diào)節(jié)血管功能^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Middle-aged (12–14 month old) Wistar male rats^[4].
Dosage:	22 mg/kg
Administration:	Oral gavage (p.o.); twice daily; 6 weeks
Result:	Caused a decrease in plasma glucose and an increase in plasma HDL cholesterol levels of the rats.

分子量

372.37

Formula

C₂₀H₂₀O₇

CAS 號

1247-97-8

性狀

固體

顏色

White to light brown

結(jié)構(gòu)分類

初始來源

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)

細(xì)胞實驗：

DMSO 中的溶解度 : 25 mg/mL (67.14 mM; 超聲助溶 (<60°C); 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	2.6855 mL	13.4275 mL	26.8550 mL
5 mM	0.5371 mL	2.6855 mL	5.3710 mL
10 mM	0.2686 mL	1.3428 mL	2.6855 mL

查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

摩爾計算器
稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動物實驗：

請根據(jù)您的實驗動物和給藥方式選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

方案一

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.71 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請依序添加每種溶劑： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.71 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

動物溶解方案計算器

請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

每只動物的給藥體積

μL

動物數(shù)量

只

由于實驗過程有損耗，建議您多配一只動物的量

請輸入您的動物體內(nèi)配方組成：

DMSO +

Tween-80 +

Saline

如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。

方案所需 助溶劑 包括：DMSO，，均可在 MCE 網(wǎng)站選購。，Tween 80，均可在 MCE 網(wǎng)站選購。

計算結(jié)果

工作液所需濃度 : mg/mL

儲備液配制方法 : mg 藥物溶于 μL DMSO（母液濃度為 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。

動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。

連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。

請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱（超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。

純度 & 產(chǎn)品資料

純度: 99.15%

選擇批次：

Data Sheet (643 KB) SDS (393 KB)

COA (289 KB) HNMR (259 KB) LCMS (94 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻(xiàn)

[1]. Jakhar R, et al. 3,5,7,3',4'-pentamethoxyflavone, a quercetin derivative protects DNA from oxidative challenges: potential mechanism of action. J Photochem Photobiol B. 2014 Feb 5;131:96-103. [Content Brief]

[2]. Jansakul C, et al. Relaxant mechanisms of 3, 5, 7, 3', 4'-pentamethoxyflavone on isolated human cavernosum. Eur J Pharmacol. 2012 Sep 15;691(1-3):235-44. [Content Brief]

[3]. Mayangsari Y, et al. 3,5,7,3',4'-Pentamethoxyflavone Enhances the Barrier Function through Transcriptional Regulation of the Tight Junction in Human Intestinal Caco-2 Cells. J Agric Food Chem. 2021 Sep 8;69(35):10174-10183. [Content Brief]

[4]. Yorsin S, et al. Increased vascular eNOS and cystathionine-γ-lyase protein after 6 weeks oral administration of 3, 5, 7, 3', 4'-pentamethoxyflavone to middle-aged male rats. Naunyn Schmiedebergs Arch Pharmacol. 2016 Nov;389(11):1183-1194. [Content Brief]

3,5,7,3′,4′-Pentamethoxyflavone 相關(guān)分類

Metabolic Disease Cardiovascular Disease

完整儲備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6855 mL	13.4275 mL	26.8550 mL	67.1375 mL
	5 mM	0.5371 mL	2.6855 mL	5.3710 mL	13.4275 mL
	10 mM	0.2686 mL	1.3428 mL	2.6855 mL	6.7138 mL
	15 mM	0.1790 mL	0.8952 mL	1.7903 mL	4.4758 mL
	20 mM	0.1343 mL	0.6714 mL	1.3428 mL	3.3569 mL
	25 mM	0.1074 mL	0.5371 mL	1.0742 mL	2.6855 mL
	30 mM	0.0895 mL	0.4476 mL	0.8952 mL	2.2379 mL
	40 mM	0.0671 mL	0.3357 mL	0.6714 mL	1.6784 mL
	50 mM	0.0537 mL	0.2686 mL	0.5371 mL	1.3428 mL
	60 mM	0.0448 mL	0.2238 mL	0.4476 mL	1.1190 mL

Help & FAQs

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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

3,5,7,3′,4′-Pentamethoxyflavone1247-97-8Calcium ChannelCa2+ channelsCa channelsCa²⁺ channelvascular functionacetylcholinediabetesInhibitorinhibitorinhibit

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3,5,7,3′,4′-Pentamethoxyflavone

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