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  2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Cell Cycle/DNA Damage
  3. Interleukin Related Reactive Oxygen Species Topoisomerase
  4. Isosteviol

Isosteviol  (Synonyms: 異甜菊醇; (-)-Isosteviol; iso-Steviol)

目錄號(hào): HY-N0872 純度: ≥98.0%
COA 產(chǎn)品使用指南 技術(shù)支持

Isosteviol ((-)-Isosteviol) 是一種甜菊糖苷衍生物，是甜菊糖苷通過酸催化水解產(chǎn)生的。Isosteviol 抑制 DNA 聚合酶和 DNA 拓?fù)洚悩?gòu)酶，并具有抗菌，抗癌和抗結(jié)核作用。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Isosteviol Chemical Structure

Isosteviol Chemical Structure

CAS No. : 27975-19-5

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥550
In-stock
50 mg ￥500
In-stock
100 mg ￥850
In-stock
200 mg   詢價(jià)  
500 mg   詢價(jià)  

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Customer Review

Other Forms of Isosteviol:

查看 Interleukin Related 亞型特異性產(chǎn)品:

查看 Topoisomerase 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Isosteviol ((-)-Isosteviol) is a derivative of Stevioside through acid catalyzed hydrolysis of Stevioside. Isosteviol inhibits DNA polymerase and DNA topoisomerase and has antibacterial, anticancer and anti-tuberculosis effects[1][2][3][4].

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
> 50 μM
Compound: 1, Isosteviol
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
[PMID: 23693151]
A549 IC50
> 50 μM
Compound: 1
Cytotoxicity against human A549 cells after 48 hrs by MTT assay
Cytotoxicity against human A549 cells after 48 hrs by MTT assay
[PMID: 23347685]
B16-F10 IC50
> 100 μM
Compound: 1, Isosteviol
Cytotoxicity against mouse B16F10 cells after 48 hrs by MTT assay
Cytotoxicity against mouse B16F10 cells after 48 hrs by MTT assay
[PMID: 24685545]
EC9706 IC50
> 100 μM
Compound: 1
Antiproliferative activity against human EC9706 cells after 72 hrs by MTT assay
Antiproliferative activity against human EC9706 cells after 72 hrs by MTT assay
[PMID: 22901386]
ECa-109 cell line IC50
> 100 μM
Compound: 1
Antiproliferative activity against human ECA109 cells after 72 hrs by MTT assay
Antiproliferative activity against human ECA109 cells after 72 hrs by MTT assay
[PMID: 22901386]
HCT-116 IC50
> 100 μM
Compound: 1; Isosteviol
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 26994841]
HCT-116 IC50
> 100 μM
Compound: 1
Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay
Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay
[PMID: 22901386]
HCT-116 IC50
> 150 μM
Compound: 1
Cytotoxicity against human HCT116 cells assessed as decrease in cell proliferation after 72 hrs by MTT assay
Cytotoxicity against human HCT116 cells assessed as decrease in cell proliferation after 72 hrs by MTT assay
[PMID: 27777008]
HeLa IC50
> 50 μM
Compound: 1, Isosteviol
Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
[PMID: 23693151]
HeLa IC50
> 50 μM
Compound: 1
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
[PMID: 23347685]
HepG2 IC50
> 100 μM
Compound: 1, Isosteviol
Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
[PMID: 24685545]
HGC-27 IC50
> 100 μM
Compound: 1; Isosteviol
Cytotoxicity against human HGC27 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human HGC27 cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 26994841]
HL-60 IC50
75 μM
Compound: 1, isosteviol
Cytotoxicity against human HL60 cells by MTT assay
Cytotoxicity against human HL60 cells by MTT assay
[PMID: 26042548]
JeKo-1 IC50
> 100 μM
Compound: 1; Isosteviol
Cytotoxicity against human JeKo1 cells assessed as growth inhibition after 72 hrs by MTT assay
Cytotoxicity against human JeKo1 cells assessed as growth inhibition after 72 hrs by MTT assay
[PMID: 26994841]
JeKo-1 IC50
> 150 μM
Compound: 1
Cytotoxicity against human JeKo1 cells assessed as decrease in cell proliferation after 72 hrs by MTT assay
Cytotoxicity against human JeKo1 cells assessed as decrease in cell proliferation after 72 hrs by MTT assay
[PMID: 27777008]
PC-3 IC50
> 100 μM
Compound: 1
Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay
Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay
[PMID: 22901386]
Raji IC50
> 50 μM
Compound: 1, Isosteviol
Antiproliferative activity against human Raji cells after 48 hrs by MTT assay
Antiproliferative activity against human Raji cells after 48 hrs by MTT assay
[PMID: 23693151]
Raji IC50
> 50 μM
Compound: 1
Cytotoxicity against human Raji cells after 48 hrs by MTT assay
Cytotoxicity against human Raji cells after 48 hrs by MTT assay
[PMID: 23347685]
Raji IC50
321 μM
Compound: 7
Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique
Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique
[PMID: 19131254]
SGC-7901 IC50
> 50 μM
Compound: 1, Isosteviol
Antiproliferative activity against human SGC7901 cells after 48 hrs by MTT assay
Antiproliferative activity against human SGC7901 cells after 48 hrs by MTT assay
[PMID: 23693151]
SGC-7901 IC50
> 50 μM
Compound: 1
Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay
Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay
[PMID: 23347685]
體外研究
(In Vitro)

Isosteviol ((-)-Isosteviol) dose-dependently relaxed the vasopressin (10-8 M)-induced vasoconstriction in isolated aortic rings with or without endothelium. However, in the presence of potassium chloride (3×10-2 M), the vasodilator effect of isosteviol on arterial strips disappeared. Only the inhibitors specific for the ATP-sensitive potassium (KATP) channel or small conductance calcium-activated potassium (SKCa) channel inhibited the vasodilator effect of isosteviol in isolated aortic rings contracted with 10-8 M vasopressin[1].
The attenuation by isosteviol of the vasopressin- and phenylephrine-induced increase in [Ca2+]i was inhibited by glibenclamide, apamin and 4-aminopyridine but not by charybdotoxin. Furthermore, the inhibitory action of isosteviol on [Ca 2+]i was blocked when A7r5 cells co-treated with glibenclamide and apamin in conjunction with 4-aminopyridine were present[2].
Isosteviol (1-100 micromol/l) inhibits angiotensin-II-induced DNA synthesis and endothelin-1 secretion. Measurements of 2'7'-dichlorofluorescin diacetate, a redox-sensitive fluorescent dye, showed an isosteviol-mediated inhibition of intracellular reactive oxygen species generated by the effects of angiotensin II[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

318.45

Formula

C20H30O3
CAS 號(hào)
性狀

固體

顏色

White to off-white

中文名稱

異甜菊醇
結(jié)構(gòu)分類
初始來源
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : ≥ 100 mg/mL (314.02 mM; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.1402 mL 15.7011 mL 31.4021 mL
5 mM 0.6280 mL 3.1402 mL 6.2804 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn)：

請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.75 mg/mL (8.64 mM); 澄清溶液

    此方案可獲得 ≥ 2.75 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 27.5 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.75 mg/mL (8.64 mM); 澄清溶液

    此方案可獲得 ≥ 2.75 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 27.5 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購(gòu)。 ，Tween 80，均可在 MCE 網(wǎng)站選購(gòu)。
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: ≥98.0%

參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1402 mL 15.7011 mL 31.4021 mL 78.5053 mL
5 mM 0.6280 mL 3.1402 mL 6.2804 mL 15.7011 mL
10 mM 0.3140 mL 1.5701 mL 3.1402 mL 7.8505 mL
15 mM 0.2093 mL 1.0467 mL 2.0935 mL 5.2337 mL
20 mM 0.1570 mL 0.7851 mL 1.5701 mL 3.9253 mL
25 mM 0.1256 mL 0.6280 mL 1.2561 mL 3.1402 mL
30 mM 0.1047 mL 0.5234 mL 1.0467 mL 2.6168 mL
40 mM 0.0785 mL 0.3925 mL 0.7851 mL 1.9626 mL
50 mM 0.0628 mL 0.3140 mL 0.6280 mL 1.5701 mL
60 mM 0.0523 mL 0.2617 mL 0.5234 mL 1.3084 mL
80 mM 0.0393 mL 0.1963 mL 0.3925 mL 0.9813 mL
100 mM 0.0314 mL 0.1570 mL 0.3140 mL 0.7851 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Isosteviol
目錄號(hào)：
HY-N0872
需求量：