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  2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease Apoptosis Anti-infection Membrane Transporter/Ion Channel Neuronal Signaling
  3. Reactive Oxygen Species Apoptosis Bacterial Fungal RSV GABA Receptor
  4. Amentoflavone

Amentoflavone  (Synonyms: 穗花杉雙黃酮; Didemethyl-ginkgetin)

目錄號(hào): HY-N0662 純度: 99.81%
COA 產(chǎn)品使用指南 技術(shù)支持

Amentoflavone (Didemethyl-ginkgetin) 是一種有效且具有口服活性的 GABA(A) 負(fù)調(diào)節(jié)劑。Amentoflavone 還顯示出抗炎、抗氧化、抗病毒、抗腫瘤、抗輻射、抗菌活性。Amentoflavone 誘導(dǎo)細(xì)胞凋亡 (apoptosis) 和細(xì)胞周期停滯在亞 G1 期。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Amentoflavone Chemical Structure

Amentoflavone Chemical Structure

CAS No. : 1617-53-4

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥829
In-stock
1 mg ￥320
In-stock
5 mg ￥700
In-stock
10 mg ￥1100
In-stock
25 mg ￥1600
In-stock
50 mg ￥2475
In-stock
100 mg ￥3900
In-stock
200 mg   詢價(jià)  
500 mg   詢價(jià)  

* Please select Quantity before adding items.

Customer Review

Other Forms of Amentoflavone:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Amentoflavone (Didemethyl-ginkgetin) is a potent and orally active GABA(A) negative modulator. Amentoflavone also shows anti-inflammatory, antioxidative, anti-viral, anti-tumor, anti-radiation, anti-fungal, antibacterial activity. Amentoflavone induces apoptosis and cell cycle arrest at sub-G1 phase[1][2][3][4].

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
8.36 μM
Compound: 10
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 29921475]
Calu-1 IC50
> 100 μM
Compound: 6
Cytotoxicity against human Calu1 cells after 3 days
Cytotoxicity against human Calu1 cells after 3 days
[PMID: 10843573]
Fibroblast IC50
1.8 μM
Compound: 4
Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs
Inhibition of UVB irradiation-induced MMP1 production in human dermal fibroblasts after 48 hrs
[PMID: 18029185]
HCT-15 IC50
> 50 μM
Compound: 8
Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human HCT15 cells assessed as cell viability after 24 hrs by MTT assay
[PMID: 23206866]
HeLa IC50
> 100 μM
Compound: 6
Cytotoxicity against human HeLa cells after 3 days
Cytotoxicity against human HeLa cells after 3 days
[PMID: 10843573]
HeLa IC50
46.4 μM
Compound: amentoflavone
Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay
Cytotoxicity against human HeLa cells assessed as growth inhibition by sulforhodamine B assay
[PMID: 26722868]
HeLa IC50
5.3 μM
Compound: 7
Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay
Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay
[PMID: 29475587]
HepG2 IC50
> 50 μM
Compound: 8
Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay
[PMID: 23206866]
HepG2 IC50
9.13 μM
Compound: 10
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 29921475]
HL-60 IC50
> 50 μM
Compound: 8
Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human HL60 cells assessed as cell viability after 24 hrs by MTT assay
[PMID: 23206866]
HL-60 IC50
51.2 μM
Compound: 7
Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay
[PMID: 29475587]
Huh-7 CC50
> 50 μM
Compound: 12
Cytotoxicity against human Huh7.5.1 cells by MTT assay
Cytotoxicity against human Huh7.5.1 cells by MTT assay
[PMID: 22445328]
Jurkat IC50
> 50 μM
Compound: 8
Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human Jurkat cells assessed as cell viability after 24 hrs by MTT assay
[PMID: 23206866]
K562 IC50
> 100 μM
Compound: 6
Cytotoxicity against human K562 cells after 3 days
Cytotoxicity against human K562 cells after 3 days
[PMID: 10843573]
MCF7 IC50
75.4 μM
Compound: 4
Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells measured after 24 hrs by MTT assay
[PMID: 34875389]
MGC-803 IC50
9.86 μM
Compound: 10
Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human MGC803 cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 29921475]
Monocyte IC50
3 μM
Compound: amentoflavone
Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
[PMID: 8882428]
Neutrophil IC50
0.75 μM
Compound: 7
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate
Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release pretreated for 5 mins followed by fMLP/CB stimulation using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate
[PMID: 29381070]
Neutrophil IC50
1.01 μM
Compound: 7
Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase
Anti-inflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation pretreated for 5 mins followed by cytochalasin B addition for 3 mins and subsequent fMLP stimulation for 10 mins by superoxide dismutase
[PMID: 29381070]
PBMC IC50
35 μM
Compound: 1
Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method
Cytotoxicity against uninfected PHA-stimulated human PBMC assessed as thymidine incorporation after 24 hrs by trypan blue exclusion method
[PMID: 9322359]
Raji IC50
> 100 μM
Compound: 6
Cytotoxicity against human Raji cells after 3 days
Cytotoxicity against human Raji cells after 3 days
[PMID: 10843573]
SW480 IC50
111 μM
Compound: 9
Cytotoxicity against human SW480 cells after 72 hrs by MTT assay
Cytotoxicity against human SW480 cells after 72 hrs by MTT assay
[PMID: 15787435]
T-24 IC50
10.67 μM
Compound: 10
Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay
Cytotoxicity against human T24 cells assessed as reduction in cell viability after 48 hrs by MTT assay
[PMID: 29921475]
THP-1 IC50
> 50 μM
Compound: 8
Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by MTT assay
[PMID: 23206866]
U-937 IC50
> 50 μM
Compound: 8
Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay
Cytotoxicity against human U937 cells assessed as cell viability after 24 hrs by MTT assay
[PMID: 23206866]
Vero IC50
> 100 μM
Compound: 6
Cytotoxicity against african green monkey Vero cells after 3 days
Cytotoxicity against african green monkey Vero cells after 3 days
[PMID: 10843573]
WISH IC50
> 100 μM
Compound: 6
Cytotoxicity against human Wish cells after 3 days
Cytotoxicity against human Wish cells after 3 days
[PMID: 10843573]
體外研究
(In Vitro)

Amentoflavone (1-60 μM) inhibits the production of nitric oxide in a concentration-dependent manner in RAW 264.7 cells[2].
? Amentoflavone (50-200 μM) inhibits the viability of U-87 MG cells with IC50 value of 100 μM at 48 h[3].
? Amentoflavone (0, 50, 100 μM; 48 h) induces apoptosis and cell cycle arrest at sub-G1 phase[3].
? Amentoflavone (0, 50, 100 μM; 48 h) inhibits NF-?B activation and decreases the expression of MCL1 and C-FLIP protein in U-87 MG cells[3].
? Amentoflavone (0-32 μg/ml) shows antibacterial activity with MICs of 8, 4, 32, 8, 16, 8 μg/ml for E. faecium ATCC 19434, S. aureus ATCC 25923, S. mutans ATCC 3065, E. coli O-157 ATCC 25922, E. coli ATCC 43895, P. aeruginosa ATCC 27853, respectively[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3]

Cell Line: U-87 MG cells
Concentration: 0, 50, 75, 100, 200 μM
Incubation Time: 48 h
Result: Significantly inhibited the viability of U-87 MG cells by 23-71% with an IC50 value of 100 μM at 48 h.

Apoptosis Analysis[3]

Cell Line: U-87 MG cells
Concentration: 0, 50, 100 μM
Incubation Time: 48 h
Result: Significantly induced the accumulation of cells in the sub-G1 population and increased the level of active caspase-3 by 14-52% and 24-42%, respectively, and significantly triggered the loss of Ψm and the expression of active caspase-8 by 23-53% and 25-50%, respectively.

Western Blot Analysis[3]

Cell Line: U-87 MG cells
Concentration: 0, 50, 100 μM
Incubation Time: 48 h
Result: Significantly reduced NF-?B activation in a dose-dependent manner by 25-87% and reduced protein expression of MCL1 and C-FLIP by 50-80% and 38-57%, respectively.
體內(nèi)研究
(In Vivo)

Amentoflavone (25 mg/kg; p.o.; once a day for 3 consecutive days) shows neuroprotective role in epilepsy via anti-inflammatory effects in mouse[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-6 weeks, 28-32 g, kunming mice[1]
Dosage: 25 mg/kg
Administration: P.o.; once a day for 3 consecutive days
Result: Inhibited activation and nuclear translocation of NF-κB subunits p65, decreased IL-6 and IL-1β production and significantly decreased NO and prostaglandin E2 production.
分子量

538.46

Formula

C30H18O10
CAS 號(hào)
性狀

固體

顏色

Light yellow to yellow

中文名稱

穗花杉雙黃酮；阿曼托黃酮
結(jié)構(gòu)分類
初始來源
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 125 mg/mL (232.14 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 1.8571 mL 9.2857 mL 18.5715 mL
5 mM 0.3714 mL 1.8571 mL 3.7143 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn)：

請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.64 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (4.64 mM); 懸濁液; 超聲助溶

    此方案可獲得 2.5 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.81%

參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8571 mL 9.2857 mL 18.5715 mL 46.4287 mL
5 mM 0.3714 mL 1.8571 mL 3.7143 mL 9.2857 mL
10 mM 0.1857 mL 0.9286 mL 1.8571 mL 4.6429 mL
15 mM 0.1238 mL 0.6190 mL 1.2381 mL 3.0952 mL
20 mM 0.0929 mL 0.4643 mL 0.9286 mL 2.3214 mL
25 mM 0.0743 mL 0.3714 mL 0.7429 mL 1.8571 mL
30 mM 0.0619 mL 0.3095 mL 0.6190 mL 1.5476 mL
40 mM 0.0464 mL 0.2321 mL 0.4643 mL 1.1607 mL
50 mM 0.0371 mL 0.1857 mL 0.3714 mL 0.9286 mL
60 mM 0.0310 mL 0.1548 mL 0.3095 mL 0.7738 mL
80 mM 0.0232 mL 0.1161 mL 0.2321 mL 0.5804 mL
100 mM 0.0186 mL 0.0929 mL 0.1857 mL 0.4643 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Amentoflavone
目錄號(hào)：
HY-N0662
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