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  2. 誘導(dǎo)疾病模型產(chǎn)品 Immunology/Inflammation Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. 免疫與炎癥疾病模型 Glucocorticoid Receptor Endogenous Metabolite
  4. 半抗原
  5. Hydrocortisone

Hydrocortisone  (Synonyms: 氫化可的松; Cortisol)

目錄號: HY-N0583 純度: 99.90%
COA 產(chǎn)品使用指南 技術(shù)支持

Hydrocortisone (Cortisol) 是腎上腺皮質(zhì)分泌的類固醇激素或糖皮質(zhì)激素。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Hydrocortisone Chemical Structure

Hydrocortisone Chemical Structure

CAS No. : 50-23-7

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥500
In-stock
500 mg ￥400
In-stock
1 g ￥500
In-stock
5 g ￥800
In-stock
10 g   詢價  
50 g   詢價  

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MCE 顧客使用本產(chǎn)品發(fā)表的 44 篇科研文獻

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Hydrocortisone (Cortisol) is a steroid hormone or glucocorticoid secreted by the adrenal cortex[1].

IC50 & Target

Human Endogenous Metabolite

 

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
> 20 μM
Compound: HYD
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
[PMID: 26695732]
B16-F10 IC50
> 20 μM
Compound: HYD
Cytotoxicity against mouse B16F10 cells after 72 hrs by MTT assay
Cytotoxicity against mouse B16F10 cells after 72 hrs by MTT assay
[PMID: 26695732]
COS-7 IC50
> 20 μM
Compound: HYD
Cytotoxicity against african green monkey COS1 cells after 72 hrs by MTT assay
Cytotoxicity against african green monkey COS1 cells after 72 hrs by MTT assay
[PMID: 26695732]
HeLa IC50
> 20 μM
Compound: HYD
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
[PMID: 26695732]
LLC-PK1 IC50
> 50 μM
Compound: Hydrocortisone
Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay
Inhibition of P-glycoprotein, human L-MDR1 expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay
[PMID: 12699389]
LLC-PK1 IC50
> 50 μM
Compound: Hydrocortisone
Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay
Inhibition of P-glycoprotein, mouse L-mdr1a expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay
[PMID: 12699389]
LLC-PK1 IC50
> 50 μM
Compound: Hydrocortisone
Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay
Inhibition of P-glycoprotein, mouse L-mdr1b expressed in LLC-PK1 epithelial cells using calcein-AM polarisation assay
[PMID: 12699389]
Macrophage IC50
10 nM
Compound: Hydrocortisone
Antiinflammatory activity in CD-1 mouse Macrophage assessed as inhibition of IFN-gamma induced NO production after 48 hrs by Griess reaction
Antiinflammatory activity in CD-1 mouse Macrophage assessed as inhibition of IFN-gamma induced NO production after 48 hrs by Griess reaction
[PMID: 21361338]
MCF7 IC50
> 20 μM
Compound: HYD
Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
[PMID: 26695732]
NIH3T3 IC50
> 20 μM
Compound: HYD
Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by MTT assay
Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by MTT assay
[PMID: 26695732]
RAW264.7 IC50
> 20 μM
Compound: HYD
Cytotoxicity against mouse RAW264.7 cells after 72 hrs by MTT assay
Cytotoxicity against mouse RAW264.7 cells after 72 hrs by MTT assay
[PMID: 26695732]
RAW264.7 IC50
3.4 μM
Compound: Hydrocortisone
Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay
Inhibition of NO production in LPS-stimulated mouse RAW264.7 cells incubated for 24 hrs by Griess reagent-based assay
[PMID: 33454546]
RAW264.7 IC50
40.64 μM
Compound: Hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by MTT assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by MTT assay
[PMID: 20879743]
RAW264.7 IC50
40.64 μM
Compound: Hydrocortisone
Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 24 hrs by Griess reaction
Inhibition of LPS-induced NO production in mouse RAW264.7 cells after 24 hrs by Griess reaction
[PMID: 23067550]
RAW264.7 IC50
40.64 μM
Compound: Hydrocortisone
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess reaction based spectrophotometry
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells after 24 hrs by Griess reaction based spectrophotometry
[PMID: 21807513]
RAW264.7 IC50
43.8 μM
Compound: Hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
[PMID: 23755850]
RAW264.7 IC50
5.5 μM
Compound: Hydrocortisone
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS-stimulation and measured after 18 hrs
Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced PGE2 production preincubated for 1 hr followed by LPS-stimulation and measured after 18 hrs
[PMID: 31126853]
RAW264.7 IC50
51.4 μM
Compound: hydrocortisone
Inhibition of LPS-induced NO production in mouse RAW264.7 cells by Griess assay
Inhibition of LPS-induced NO production in mouse RAW264.7 cells by Griess assay
[PMID: 25275213]
RAW264.7 IC50
52.43 μM
Compound: Hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 3 hrs followed by LPS addition measured after 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 3 hrs followed by LPS addition measured after 24 hrs by Griess assay
[PMID: 31009914]
RAW264.7 IC50
53.7 μM
Compound: hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of NO production after 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of NO production after 24 hrs by Griess assay
[PMID: 25076059]
RAW264.7 IC50
53.8 μM
Compound: Hydrocortisone
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells treated 1 hr before LPS challenge measured after 24 hrs by Griess reaction method
Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells treated 1 hr before LPS challenge measured after 24 hrs by Griess reaction method
[PMID: 23294419]
RAW264.7 IC50
54.23 μM
Compound: Hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess method
[PMID: 26615888]
RAW264.7 IC50
54.23 μM
Compound: Hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method
[PMID: 24660966]
RAW264.7 IC50
58.79 μM
Compound: Hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
[PMID: 27295506]
RAW264.7 IC50
58.79 μM
Compound: Hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay
[PMID: 27256914]
RAW264.7 IC50
58.79 μM
Compound: Hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS addition and measured after 24 hrs by griess assay
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 3 hrs followed by LPS addition and measured after 24 hrs by griess assay
[PMID: 30419492]
RAW264.7 IC50
61 nM
Compound: Hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma induced NO production after 24 hrs by Griess reaction
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma induced NO production after 24 hrs by Griess reaction
[PMID: 21361338]
RAW264.7 IC50
63.86 μM
Compound: hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-6 production by ELISA
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL-6 production by ELISA
[PMID: 20095629]
RAW264.7 IC50
64.34 μM
Compound: hydrocortisone
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition LPS-induced nitric oxide production cells after 24 hrs
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition LPS-induced nitric oxide production cells after 24 hrs
[PMID: 20095629]
RAW264.7 IC50
64.34 μM
Compound: Hydrocortisone
Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method
Inhibition of nitric oxide production in lipopolysaccharide-activated mouse RAW264.7 cells by Griess reaction based method
[PMID: 23305920]
RAW264.7 IC50
85.64 μM
Compound: hydrocortisone
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production by ELISA
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production by ELISA
[PMID: 20095629]
SK-OV-3 IC50
> 20 μM
Compound: HYD
Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay
Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay
[PMID: 26695732]
體外研究
(In Vitro)

Hydrocortisone (50 nM) 顯示 hCMEC/D3 細胞中 GR 轉(zhuǎn)錄物的劑量依賴性下調(diào)。在血清減少的細胞分化培養(yǎng)基中補充 Hydrocortisone 導(dǎo)致 hCMEC/D3 單層中的 TER 顯著增加[1]。Hydrocortisone 處理的樹突狀細胞 (DC) 顯示 MHC II 分子、共刺激分子 CD86 和 DC 特異性標記物 CD83 的表達減少，以及 IL-12 分泌的強烈減少。Hydrocortisone 處理的 DC 抑制 IFN-γ 的產(chǎn)生，但誘導(dǎo) IL-4 釋放增加，IL-5 沒有變化[2]。Hydrocortisone 可降低缺血后氧化應(yīng)激、灌注壓和滲出液形成。Hydrocortisone 抑制 syndecan-1、硫酸乙酰肝素和乙酰透明質(zhì)酸的缺血后脫落，以及常駐肥大細胞釋放組胺[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

362.46

Formula

C21H30O5
CAS 號
性狀

固體

顏色

White to off-white

中文名稱

氫化可的松；可的索；可的唑；皮質(zhì)醇；氫可的松；17-羥基皮質(zhì) (甾) 酮；皮質(zhì)甾醇；氫化皮質(zhì)素
結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : ≥ 100 mg/mL (275.89 mM; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

* "≥" means soluble, but saturation unknown.

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.7589 mL 13.7946 mL 27.5893 mL
5 mM 0.5518 mL 2.7589 mL 5.5179 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.90 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 5% DMSO    40% PEG300    5% Tween-80    50% Saline

    Solubility: ≥ 2.5 mg/mL (6.90 mM); 澄清溶液

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.90%

參考文獻
Cell Assay
[1]

Cells are plated on top of collagen IV-coated transwell chambers for six-well plates (24 mm diameter, membrane material: polyethylene terephthalate (PET), 0.4 μm pores, pore density 1.6×106?cm2) at densities of 2.5×104?cells cm2?per well. When they have reached confluence at day 5, the different experimental sets of cells are transferred to differentiation medium containing reduced amounts of FCS and treated with TNFα or hydrocortisone as indicated.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7589 mL 13.7946 mL 27.5893 mL 68.9731 mL
5 mM 0.5518 mL 2.7589 mL 5.5179 mL 13.7946 mL
10 mM 0.2759 mL 1.3795 mL 2.7589 mL 6.8973 mL
15 mM 0.1839 mL 0.9196 mL 1.8393 mL 4.5982 mL
20 mM 0.1379 mL 0.6897 mL 1.3795 mL 3.4487 mL
25 mM 0.1104 mL 0.5518 mL 1.1036 mL 2.7589 mL
30 mM 0.0920 mL 0.4598 mL 0.9196 mL 2.2991 mL
40 mM 0.0690 mL 0.3449 mL 0.6897 mL 1.7243 mL
50 mM 0.0552 mL 0.2759 mL 0.5518 mL 1.3795 mL
60 mM 0.0460 mL 0.2299 mL 0.4598 mL 1.1496 mL
80 mM 0.0345 mL 0.1724 mL 0.3449 mL 0.8622 mL
100 mM 0.0276 mL 0.1379 mL 0.2759 mL 0.6897 mL
Help & FAQs
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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Hydrocortisone
目錄號：
HY-N0583
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