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  2. Apoptosis Others
  3. Apoptosis Fluorescent Dye
  4. Umbelliferone

Umbelliferone  (Synonyms: 7-羥基香豆素; 7-Hydroxycoumarin; Hydrangin; NSC 19790)

目錄號(hào): HY-N0573 純度: 99.67%
COA 產(chǎn)品使用指南 技術(shù)支持

Umbelliferone (7-Hydroxycoumarin) 是香豆素家族的天然產(chǎn)物，具有口服活性，是一種熒光化合物，可用作防曬劑。 Umbelliferone 誘導(dǎo) HepG2 細(xì)胞的細(xì)胞周期停滯，凋亡 (apoptosis) 和 DNA 片段化。Umbelliferone 具有顯著的抗癌作用。Umbelliferone 可以減輕過(guò)敏性氣道炎癥的改變特征。Umbelliferone 顯示出神經(jīng)保護(hù)作用并穿過(guò)血腦屏障。Umbelliferone 在慢性酒精喂養(yǎng)的大鼠中表現(xiàn)出抗炎和抗氧化作用。

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Umbelliferone Chemical Structure

Umbelliferone Chemical Structure

CAS No. : 93-35-6

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥550
In-stock
100 mg ￥500
In-stock
200 mg   詢價(jià)  
500 mg   詢價(jià)  

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Customer Review

Other Forms of Umbelliferone:

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻(xiàn)

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Umbelliferone (7-Hydroxycoumarin), a natural orally active product of the coumarin family, is a fluorescing compound which can be used as a sunscreen agent. Umbelliferone induces cell cycle arrest, apoptosis and DNA fragmentation in HepG2 cells. Umbelliferone exhibits significant anticancer effects. Umbelliferone attenuates the alteration characteristics of allergic airway inflammation. Umbelliferone displays the neuroprotective effects and cross the blood-brain barrier. Umbelliferone exhibits anti-inflammatory and antioxidant effects in chronic alcohol-fed rats[1][2][3][4].

IC50 & Target

Human Endogenous Metabolite

 

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
> 100 μM
Compound: 6
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
Cytotoxicity against human A549 cells after 72 hrs by MTT assay
[PMID: 21696954]
A549 IC50
21.11 μM
Compound: 7-C
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
Antiproliferative activity against human A549 cells after 48 hrs by MTT assay
[PMID: 28720504]
BT-549 IC50
1 mM
Compound: 5
Antiproliferative activity against human BT549 cells after 24 hrs by MTT assay
Antiproliferative activity against human BT549 cells after 24 hrs by MTT assay
[PMID: 29144746]
HCT-116 IC50
8.05 μM
Compound: 7
Cytotoxicity against human HCT116 cells after 96 hrs by MTT assay
Cytotoxicity against human HCT116 cells after 96 hrs by MTT assay
[PMID: 23746477]
HEK-293T IC50
189.63 μM
Compound: 7-C
Antiproliferative activity against human 293T cells after 48 hrs by MTT assay
Antiproliferative activity against human 293T cells after 48 hrs by MTT assay
[PMID: 28720504]
HeLa IC50
42.35 μM
Compound: 7-C
Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay
[PMID: 28720504]
HT-29 IC50
22.67 μM
Compound: 7-C
Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay
Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay
[PMID: 28720504]
HT-29 IC50
4.35 μM
Compound: 7
Cytotoxicity against human HT-29 cells after 96 hrs by MTT assay
Cytotoxicity against human HT-29 cells after 96 hrs by MTT assay
[PMID: 23746477]
LNCaP IC50
> 20 μg/mL
Compound: 1
Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)
Inhibitory activity against 5-alpha-reductase type 1 in cell culture system using LNCaP cells (androgen-sensitive human prostatic cancer cell line)
[PMID: 11527731]
LoVo IC50
97 μM
Compound: 6
Cytotoxicity against human LoVo cells after 72 hrs by MTT assay
Cytotoxicity against human LoVo cells after 72 hrs by MTT assay
[PMID: 21696954]
MCF7 IC50
2.9 μM
Compound: 5
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
Antiproliferative activity against human MCF7 cells after 24 hrs by MTT assay
[PMID: 29144746]
MDA-MB-231 IC50
3.7 μM
Compound: 5
Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay
[PMID: 29144746]
MT4 IC50
2341 μM
Compound: 7
Cytotoxicity against human MT4 cells after 96 hrs by MTT assay
Cytotoxicity against human MT4 cells after 96 hrs by MTT assay
[PMID: 23746477]
PC-3 IC50
> 100 μM
Compound: 6
Cytotoxicity against human PC3 cells after 72 hrs by MTT assay
Cytotoxicity against human PC3 cells after 72 hrs by MTT assay
[PMID: 21696954]
SK-MEL-28 IC50
> 100 μM
Compound: 6
Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay
Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay
[PMID: 21696954]
SK-MEL-28 IC50
0.48 μM
Compound: 5
Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 37197457]
SK-MEL3 IC50
1.6 μM
Compound: 5
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs in presence of Artesunic acid by MTT assay
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs in presence of Artesunic acid by MTT assay
[PMID: 37197457]
SK-MEL3 IC50
2.1 μM
Compound: 5
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 37197457]
SMMC-7721 IC50
19.9 μM
Compound: 7-C
Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay
Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay
[PMID: 28720504]
U-373MG ATCC IC50
> 100 μM
Compound: 6
Cytotoxicity against human U373 cells after 72 hrs by MTT assay
Cytotoxicity against human U373 cells after 72 hrs by MTT assay
[PMID: 21696954]
體外研究
(In Vitro)

Umbelliferone (1-50 μM, 12-48 h) 誘導(dǎo) HepG2 細(xì)胞活力降低并呈現(xiàn)劑量和時(shí)間依賴性[1]。
Umbelliferone (0-50 μM, 24 h) 導(dǎo)致 HepG2 細(xì)胞收縮，膜起泡，核濃縮和凋亡小體形成[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[1]

Cell Line: HepG2 cells
Concentration: 0, 5, 25 50 μM
Incubation Time: 24 h
Result: Resulted in the appearance of cell shrinkage and membrane blebbing, when compared with the morphology of untreated cells.
At 50 μM almost all the HepG2 cancer cells shrank significantly and no cells with normal morphological features were detected.
Reduced large nuclei.
Induced nuclear condensation and apoptotic body formation.

Cell Cycle Analysis[1]

Cell Line: HepG2 cells
Concentration: 0, 5, 25 50 μM
Incubation Time: 24 h
Result: Increased in the proportion of cells in S phase (45.0, 51.3 and 62.1%, compared with 43.6% in untreated cells) and reduced in the fraction of cells in G1 phase (42.1, 45.6 and 32.2%, compared with 53.2% in the untreated cells).
體內(nèi)研究
(In Vivo)

Umbelliferone (90 mg/kg, p.o., 每日, 5 天) 可減少受攻擊小鼠支氣管肺泡灌洗液中的總細(xì)胞，中性粒細(xì)胞，嗜酸性粒細(xì)胞和單核細(xì)胞的數(shù)量[2]。
Umbelliferone (15-30mg/kg, i.g., 每日, 21 天) 對(duì) CUMS (慢性不可預(yù)測(cè)的輕度應(yīng)激) 誘導(dǎo)的抑郁癥鼠模型顯示出神經(jīng)保護(hù)作用，這與抑制 ROCK/Akt 通路調(diào)節(jié)的神經(jīng)元凋亡有關(guān)[3]。
Umbelliferone (0.05 g/L, 口服, 每日, 8 周) 通過(guò)抑制 TLR4 信號(hào)通路和激活抗氧化系統(tǒng)來(lái)預(yù)防用含5%酒精的利伯德卡里流質(zhì)飲食誘導(dǎo)大鼠的肝損傷[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male BALB/c mice, 4-6 weeks old received systemic immunization by subcutaneous injection of 10 μg of chicken egg ovalbumin diluted in 2 mg/mL alum followed by a booster injection at day 14. A nasal challenge was performed starting at day 28, by inhalational exposure to aerosolised ovalbumin for 15 min/day, on five consecutive days[2].
Dosage: 30, 60, 90 mg/kg
Administration: p.o., daily, 5 days
Result: Mice sensitized and challenged with ovalbumin have increased the number of inflammatory cells in bronchoalveolar lavage fluid.
Reduced the number of total cells and of neutrophils in the bronchoalveolar lavage fluid.
At 60 and 90 mg/kg, but not at 30 mg/kg, decreased significantly the number of eosinophils found in the bronchoalveolar lavage fluid of challenged mice.
At 60 mg/kg, decreased the number of mononuclear cells.
Caused a reduction in the levels of Th2-associated cytokines (IL-4, IL-5, IL-13).
Animal Model: While the control group of rats without receiving stress treatment had free access to food and water, other groups of rats were subjected to different types of stressors: cage tilting for 24 h, damp sawdust for 24 h, predator sounds, swimming in 4 ℃ cold water for 5min, swimming in 45 ℃ hot water for 5min, fasting for 48 h, water deprivation for 24 h, shaking for 15 min, nip trail for 1 min[3][5].
Dosage: 15,30 mg/kg
Administration: i.g., daily, 21 days
Result: Exhibited an antidepressant-like effect, as sucrose consumption in CUMS rats significantly increased.
Decreased the expression of caspase3 and bax and increased the expression of bcl2 in CA1 region of hippocampus compared to the control group.
Remarkably increased the number of nissl-positive cells.
Down-regulated the levels of inflammatory cytokines IL-1β, IL-6 and TNF-α in hippocampus in the CUMS group versus control group.
Decreased the protein level of ROCK2 and phosphorylation expression of PTEN stimulated by CUMS.
Up-regulated the phosphorylation expressions of Akt and GSK3.
Animal Model: Rats were fed a Liber-Decarli liquid diet containing 5% alcohol for 8 weeks, while normal rats received an isocaloric carbohydrate liquid diet[4].
Dosage: 0.05 g/L
Administration: p.o., daily, 8 weeks
Result: Decreased in serum TNF-α and IL-6 levels and increased IL-10 level.
Decreased the mRNA expressions of LBP, TLR4, NF-κB and TNF-α and IL-6.
Up-regulated SOD and CAT mRNA levels and activity.
Increased H2O2 and GSH levels and decreased hepatic MDA (Malondialdehyde) levels.
分子量

162.14

Formula

C9H6O3
CAS 號(hào)
性狀

固體

顏色

Light yellow to orange

中文名稱

7-羥基香豆素
結(jié)構(gòu)分類
初始來(lái)源
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 100 mg/mL (616.74 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 6.1674 mL 30.8368 mL 61.6736 mL
5 mM 1.2335 mL 6.1674 mL 12.3347 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn)：

請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

  • 方案 一

    請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 1.43 mg/mL (8.82 mM); 澄清溶液; 超聲助溶

    此方案可獲得 1.43 mg/mL的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 14.3 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 1.43 mg/mL (8.82 mM); 澄清溶液; 超聲助溶

    此方案可獲得 1.43 mg/mL的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 14.3 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量
請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過(guò) 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購(gòu)。 ，Tween 80，均可在 MCE 網(wǎng)站選購(gòu)。
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過(guò)半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.67%

參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.1674 mL 30.8368 mL 61.6736 mL 154.1839 mL
5 mM 1.2335 mL 6.1674 mL 12.3347 mL 30.8368 mL
10 mM 0.6167 mL 3.0837 mL 6.1674 mL 15.4184 mL
15 mM 0.4112 mL 2.0558 mL 4.1116 mL 10.2789 mL
20 mM 0.3084 mL 1.5418 mL 3.0837 mL 7.7092 mL
25 mM 0.2467 mL 1.2335 mL 2.4669 mL 6.1674 mL
30 mM 0.2056 mL 1.0279 mL 2.0558 mL 5.1395 mL
40 mM 0.1542 mL 0.7709 mL 1.5418 mL 3.8546 mL
50 mM 0.1233 mL 0.6167 mL 1.2335 mL 3.0837 mL
60 mM 0.1028 mL 0.5139 mL 1.0279 mL 2.5697 mL
80 mM 0.0771 mL 0.3855 mL 0.7709 mL 1.9273 mL
100 mM 0.0617 mL 0.3084 mL 0.6167 mL 1.5418 mL
Help & FAQs
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產(chǎn)品名稱：
Umbelliferone
目錄號(hào)：
HY-N0573
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