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  2. Immunology/Inflammation
  3. NOD-like Receptor (NLR)
  4. Ruscogenin

Ruscogenin  (Synonyms: 魯斯可皂苷元)

目錄號(hào): HY-N0496 純度: 99.59%
COA 產(chǎn)品使用指南 技術(shù)支持

Ruscogenin 是從 Ophiopogon japonicus 提取的一種重要甾體皂苷元，通過抑制 TXNIP/NLRP3 炎癥體激活和 MAPK 途徑減輕腦缺血誘導(dǎo)的血腦屏障功能障礙，Ruscogenin 具有顯著的抗炎和抗血栓活性。Ruscogenin 有口服活性。

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Ruscogenin Chemical Structure

Ruscogenin Chemical Structure

CAS No. : 472-11-7

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥805
In-stock
1 mg ￥377
In-stock
5 mg ￥850
In-stock
10 mg ￥1318
In-stock
25 mg ￥2163
In-stock
50 mg ￥3225
In-stock
100 mg ￥4738
In-stock
200 mg   詢價(jià)  
500 mg   詢價(jià)  

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Customer Review

Other Forms of Ruscogenin:

查看 NOD-like Receptor (NLR) 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Ruscogenin, an important steroid sapogenin derived from Ophiopogon japonicus, attenuates cerebral ischemia-induced blood-brain barrier dysfunction by suppressing TXNIP/NLRP3 inflammasome activation and the MAPK pathway. Ruscogenin exerts significant anti-inflammatory and anti-thrombotic activities. Ruscogenin has orally bioactivity[1][2].

IC50 & Target

NLRP3

 

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
BXPC-3 IC50
7.32 μM
Compound: 48
Anticancer activity against human BXPC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Anticancer activity against human BXPC-3 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 36332549]
SW1990 IC50
8.14 μM
Compound: 48
Anticancer activity against human SW1990 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Anticancer activity against human SW1990 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
[PMID: 36332549]
THP-1 IC50
2.27 μM
Compound: 1
Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
[PMID: 20561785]
體外研究
(In Vitro)

Ruscogenin (0.01-40 μM；24 小時(shí)) 可提高 OGD/R 處理下 bEnd.3 細(xì)胞的細(xì)胞活力[1]。
Ruscogenin (0.01-10 μM；24 小時(shí)) 在經(jīng) OGD/R 處理的 bEnd.3 細(xì)胞中可恢復(fù)內(nèi)皮細(xì)胞屏障滲漏，抑制 IL-Iβ 和 Caspase-1 的表達(dá)，并降低 TXNIP/NLRP3 通路信號(hào)[1]。
Ruscogenin（0.01-10 μM； 4 小時(shí)）在經(jīng) OGD/R 處理的 bEnd.3 細(xì)胞中可抑制 ROS 的產(chǎn)生，并降低 MAPK 通路的信號(hào)[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: bEnd.3 cell
Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 24 h
Result: Showed that the cell viability reduction in OGD/R-induced bEnd.3 cell was significantly recovered.

Immunofluorescence[1]

Cell Line: bEnd.3 cell
Concentration: 0.1 μM, 1 μM, 10 μM
Incubation Time: 24 h
Result: Demonstrated increase in the TEER value and inhibition the sodium fluorescein permeability.

Western Blot Analysis[1]

Cell Line: bEnd.3 cell
Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 24 h
Result: Demonstrated downregulation of the expression of IL-1β and Caspase-1 proteins, and inhibition in the expressions of NLRP3 and TXNIP.

Immunofluorescence[1]

Cell Line: bEnd.3 cell
Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 4 h
Result: Demonstrated reduction in the production of ROS.
體內(nèi)研究
(In Vivo)

Ruscogenin (10 mg/kg；灌胃；1次) 改善 MCAO/R 誘導(dǎo)的腦組織損傷，抑制 IL-1β 和 Caspase-1 的表達(dá)并抑制TXNIP/NLRP3 的激活[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MCAO/R Mice[1]
Dosage: 10mg/kg
Administration: Oral Gavage (i.g.)
Result: Showed smaller infarct size, ameliorating histopathological damage by decreasing the cell loss, and significant increase in CBF (cerebral blood flow) compared to the model group.
Resulted inhibiting the expression of IL-1β and Caspase-1, NLRP3 and TXNIP compared to the model group.
分子量

430.62

Formula

C27H42O4
CAS 號(hào)
性狀

固體

顏色

White to off-white

中文名稱

魯斯可皂苷元；螺可吉寧
結(jié)構(gòu)分類
初始來源
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

Ethanol 中的溶解度 : 25 mg/mL (58.06 mM; 超聲助溶)

DMSO 中的溶解度 : 4.31 mg/mL (10.01 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.3222 mL 11.6112 mL 23.2223 mL
5 mM 0.4644 mL 2.3222 mL 4.6445 mL
查看完整儲(chǔ)備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲(chǔ)存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn)：

以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

  • 方案 一

    請依序添加每種溶劑： 50% PEG300    50% Saline

    Solubility: 1.67 mg/mL (3.88 mM); 懸濁液; 超聲助溶

動(dòng)物溶解方案計(jì)算器
請輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料

純度: 99.59%

參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲(chǔ)存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO / Ethanol 1 mM 2.3222 mL 11.6112 mL 23.2223 mL 58.0558 mL
5 mM 0.4644 mL 2.3222 mL 4.6445 mL 11.6112 mL
10 mM 0.2322 mL 1.1611 mL 2.3222 mL 5.8056 mL
Ethanol 15 mM 0.1548 mL 0.7741 mL 1.5482 mL 3.8704 mL
20 mM 0.1161 mL 0.5806 mL 1.1611 mL 2.9028 mL
25 mM 0.0929 mL 0.4644 mL 0.9289 mL 2.3222 mL
30 mM 0.0774 mL 0.3870 mL 0.7741 mL 1.9352 mL
40 mM 0.0581 mL 0.2903 mL 0.5806 mL 1.4514 mL
50 mM 0.0464 mL 0.2322 mL 0.4644 mL 1.1611 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Ruscogenin
目錄號(hào)：
HY-N0496
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