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  2. MAPK/ERK Pathway Autophagy Metabolic Enzyme/Protease
  3. Carboxylesterase p38 MAPK Autophagy UGT
  4. Bakuchiol

Bakuchiol  (Synonyms: 補骨脂酚; (S)-(+)-Bakuchiol)

目錄號: HY-N0235 純度: 98.53%
COA 產(chǎn)品使用指南

Bakuchiol 是一種可以從補骨脂種子中得到的植物雌激素。Bakuchiol 被證實為多種酶的非競爭性抑制劑，包括 UDP-葡萄糖醛酸基轉(zhuǎn)移酶 2B7 （UGT2B7） 和人羧酸酯酶 2 （hCE2），IC50s 值分別為 40.9 μM 和 7.28 μM。Bakuchiol 在抗炎、抗菌、抗腫瘤以及藥物代謝調(diào)控等方面具有極大的研究和應用潛力。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務

Bakuchiol Chemical Structure

Bakuchiol Chemical Structure

CAS No. : 10309-37-2

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥242
In-stock
5 mg ￥220
In-stock
10 mg ￥350
In-stock
25 mg ￥600
In-stock
50 mg ￥950
In-stock
100 mg ￥1400
In-stock
500 mg ￥3588
In-stock
1 g   詢價  
5 g   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Bakuchiol:

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻

查看 p38 MAPK 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Bakuchiol is a phytoestrogen that can be obtained from psoralen seeds. Bakuchiol has been proven to be a non-competitive inhibitor of multiple enzymes, including UDP-glucuronosyltransferase 2B7 (UGT2B7) [2] and human carboxylesterase 2 (hCE2) [3], with IC50s values of 40.9 μM and 7.28 μM, respectively. Bakuchiol exhibits significant research and application potential in areas such as anti-inflammatory[5], antibacterial[4], antitumor[1] therapies, as well as drug metabolism regulation.

IC50 & Target

IC50:40.9 nM (UGT2B7) ; IC50:7.28 nM (hCE2)
細胞效力
(Cellular Effect)
Cell Line Type Value Description References
AGS IC50
15.3 μM
Compound: 1
Cytotoxicity against human AGS cells by MTT assay
Cytotoxicity against human AGS cells by MTT assay
[PMID: 18359631]
AGS IC50
6.1 μM
Compound: 1
Inhibition of hypoxia-induced HIF1 activation in human AGS cells by reporter gene assay
Inhibition of hypoxia-induced HIF1 activation in human AGS cells by reporter gene assay
[PMID: 18359631]
BJ EC50
> 36.63 μM
Compound: 1
Cytotoxicity against human BJ cells after 72 hrs by Cell-Titer Glo assay
Cytotoxicity against human BJ cells after 72 hrs by Cell-Titer Glo assay
[PMID: 26922230]
HEK293 EC50
> 36.63 μM
Compound: 1
Cytotoxicity against HEK293 cells after 72 hrs by Cell-Titer Glo assay
Cytotoxicity against HEK293 cells after 72 hrs by Cell-Titer Glo assay
[PMID: 26922230]
HeLa IC50
11 μM
Compound: 1
Cytotoxicity against human HeLa cells by MTT assay
Cytotoxicity against human HeLa cells by MTT assay
[PMID: 18359631]
HepG2 EC50
> 52.0833 μM
Compound: 1
Cytotoxicity against human HepG2 cells after 72 hrs by Cell-Titer Glo assay
Cytotoxicity against human HepG2 cells after 72 hrs by Cell-Titer Glo assay
[PMID: 26922230]
Jurkat EC50
> 49.0196 μM
Compound: 1
Cytotoxicity against human Jurkat cells after 72 hrs by Cell-Titer Glo assay
Cytotoxicity against human Jurkat cells after 72 hrs by Cell-Titer Glo assay
[PMID: 26922230]
NALM-6 EC50
38 μM
Compound: 1
Cytotoxicity against human NALM6 cells after 72 hrs by Cell-Titer Glo assay
Cytotoxicity against human NALM6 cells after 72 hrs by Cell-Titer Glo assay
[PMID: 26922230]
Raji EC50
> 26.0417 μM
Compound: 1
Cytotoxicity against human Raji cells after 72 hrs by Cell-Titer Glo assay
Cytotoxicity against human Raji cells after 72 hrs by Cell-Titer Glo assay
[PMID: 26922230]
RAW264.7 IC50
97.23 μM
Compound: BAK
Cytotoxicity against mouse RAW 264.7 cells assessed as decreased cell viability measured after 24 hrs by MTT assay
Cytotoxicity against mouse RAW 264.7 cells assessed as decreased cell viability measured after 24 hrs by MTT assay
[PMID: 35000392]
SEM EC50
15.2866 μM
Compound: 1
Cytotoxicity against human SEM cells after 72 hrs by Cell-Titer Glo assay
Cytotoxicity against human SEM cells after 72 hrs by Cell-Titer Glo assay
[PMID: 26922230]
體外研究
(In Vitro)

Bakuchiol (5-20 μM, 72 h) 通過 MTT 實驗發(fā)現(xiàn)對 A549 細胞具有選擇性的細胞毒性。Bakuchiolb在降低 A549 細胞活力方面比它的同類物質(zhì) Resveratrol (HY-16561) 更為有效，它們的 IC50s 值分別為 9.58 μM 和 33.02 μM[1]。
Bakuchiol (5-20 μM, 24 h or 36h) 能呈濃度依賴性地將 A549 細胞的細胞周期進程阻滯至 S 期，并誘導活性氧 (ROS) 相關(guān)的細胞凋亡[1]。
Bakuchiol (0-80 μM, 120 min) 是一種針對 UDP-葡萄糖醛酸基轉(zhuǎn)移酶 2B7 (UGT2B7) 的非競爭性抑制劑，其 Ki 值為 10.7 μM，IC50 值為 40.9 μM[2]。
Bakuchiol (30 min) 是一種針對人羧酸酯酶 2 (hCE2) 的強效的非競爭性抑制劑，其 Ki 值為 2.12 μM，IC50 值為 7.28 μM[3]。
Bakuchiol (0.9775-3.91 μg/mL, 24 h) 能呈濃度依賴性地增加 T. mentagrophytes 真菌分生孢子的膜通透性[4]。
Bakuchiol (3.91 μg/mL, 3 h) 能夠引發(fā)真菌細胞中 187 % 的活性氧 (ROS) 水平升高[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: A549 cells, EA.hy926 cells, HUVEC cells and MEF
Concentration: 5, 10, and 20 μM
Incubation Time: 24 hours
Result: Induced caspase 9/3 activaton, p53 and Bax up-regulation, as well as Bcl-2 down-regulation.

Apoptosis Analysis[1]

Cell Line: A549 cells, EA.hy926 cells, HUVEC cells and MEF
Concentration: 5, 10, and 20 μM
Incubation Time: 36 hours
Result: Resulted in typical apoptotic cells (p < 0.05), as determined by Annexin V/propidium iodide staining and flow cytometry.

Cell Cycle Analysis[1]

Cell Line: A549 cells
Concentration: 5, 10, and 20 μM
Incubation Time: 24 hours
Result: Increased S phase cell population accompanied with a concomitant reduction of cells in the G1 phase. Resulted in a less potent effect in S phase arrest than bakuchiol.

Cell Cytotoxicity Assay[1]

Cell Line: A549 cells, EA.hy926 cells, HUVEC cells and MEF
Concentration: 5, 10, and 20 μM
Incubation Time: 72 hours
Result: Inhibited the growth of A549 cells, while the growth rate of other cells were not changed. Exhibited concentration-dependent cytotoxicity in A549 cells, while bakuchiol showed a more potent effect than that of resveratrol with IC50s vales of 9.58 μmol/L and 33.02 μmol/ L, respectively.
體內(nèi)研究
(In Vivo)

Bakuchiol (10 and 20 mg/kg, p.o.) 可以減輕卵清蛋白（OVA）誘導的過敏性鼻炎 （AR）小鼠的過敏癥狀，減少炎癥反應，改善 T 細胞平衡，減少氧化應激并調(diào)節(jié) Igs 水平[5]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: OVA -induced AR model (BALB/c mice, 6-week-old) [5]
Dosage: 10 and 20 mg/kg
Administration: Oral route (p.o.)
Result: Reduced the nasal symptoms and decreased the levels of IL-4, IL-5, IL-13, Igs (IgE and IgG1), histamine, IL-10, IL-33, and TNF-α. Reduced PGDA and LTC-4 levels in the nasal lavage fluid (NLF). Decreased the ROS and MDA levels, whereas boosting SOD activity. Decreased the eosinophil count in the nasal tissues and influenced the Th1 and Th2 cell proportions.
Clinical Trial
分子量

256.38

Formula

C18H24O
CAS 號
性狀

液體（密度：0.963 g/cm3）

顏色

Colorless to yellow

中文名稱

補骨脂酚
結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : 62.5 mg/mL (243.78 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.9005 mL 19.5023 mL 39.0046 mL
5 mM 0.7801 mL 3.9005 mL 7.8009 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.17 mg/mL (8.46 mM); 澄清溶液

    此方案可獲得 ≥ 2.17 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 21.7 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    90% Corn Oil

    Solubility: ≥ 2.17 mg/mL (8.46 mM); 澄清溶液

    此方案可獲得 ≥ 2.17 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。

    以 1 mL 工作液為例，取 100 μL 21.7 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.25%

參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.9005 mL 19.5023 mL 39.0046 mL 97.5115 mL
5 mM 0.7801 mL 3.9005 mL 7.8009 mL 19.5023 mL
10 mM 0.3900 mL 1.9502 mL 3.9005 mL 9.7512 mL
15 mM 0.2600 mL 1.3002 mL 2.6003 mL 6.5008 mL
20 mM 0.1950 mL 0.9751 mL 1.9502 mL 4.8756 mL
25 mM 0.1560 mL 0.7801 mL 1.5602 mL 3.9005 mL
30 mM 0.1300 mL 0.6501 mL 1.3002 mL 3.2504 mL
40 mM 0.0975 mL 0.4876 mL 0.9751 mL 2.4378 mL
50 mM 0.0780 mL 0.3900 mL 0.7801 mL 1.9502 mL
60 mM 0.0650 mL 0.3250 mL 0.6501 mL 1.6252 mL
80 mM 0.0488 mL 0.2438 mL 0.4876 mL 1.2189 mL
100 mM 0.0390 mL 0.1950 mL 0.3900 mL 0.9751 mL
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產(chǎn)品名稱：
Bakuchiol
目錄號：
HY-N0235
需求量：