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  2. Cell Cycle/DNA Damage Epigenetics Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor Apoptosis
  3. Sirtuin PPAR TNF Receptor
  4. Fisetin

Fisetin是一種在許多水果和蔬菜中發(fā)現(xiàn)的天然黃酮醇，具有多種益處，如抗氧化，抗癌，神經(jīng)保護(hù)作用。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Fisetin Chemical Structure

Fisetin Chemical Structure

CAS No. : 528-48-3

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥550
In-stock
100 mg ￥500
In-stock
500 mg ￥700
In-stock
1 g ￥1100
In-stock
5 g   詢價  
10 g   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Fisetin:

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Fisetin is a natural flavonol found in many fruits and vegetables with various benefits, such as antioxidant, anticancer, neuroprotection effects.

IC50 & Target

Sirtuin, PPAR, TNF-alpha[1][2]
細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
BV-2 IC50
13.5 μM
Compound: Fisetin
Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pretreated for 1 hr followed by LPS addition and measured after 24 hrs by Griess assay
Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pretreated for 1 hr followed by LPS addition and measured after 24 hrs by Griess assay
[PMID: 28923363]
BV-2 IC50
73.02 μM
Compound: 13
Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay
[PMID: 28911817]
CWR22R IC50
25.9 μM
Compound: 22
Antiproliferative activity against human 22Rv1 cells incubated up to 144 hrs by Coulter particle count and size analyzer
Antiproliferative activity against human 22Rv1 cells incubated up to 144 hrs by Coulter particle count and size analyzer
[PMID: 22789812]
DU-145 IC50
> 50 μM
Compound: Fisetin
Antiproliferative activity against human DU145 cells after 3 days by WST-1 assay
Antiproliferative activity against human DU145 cells after 3 days by WST-1 assay
[PMID: 30165345]
DU-145 IC50
> 50 μM
Compound: 2
Antiproliferative activity against human DU145 cells after 3 days by WST-1 assay
Antiproliferative activity against human DU145 cells after 3 days by WST-1 assay
[PMID: 28760528]
H9 EC50
122 μM
Compound: 14
Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay
Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay
[PMID: 8158164]
H9 IC50
157 μM
Compound: 14
Cytotoxicity against human H9 cells after 3 days
Cytotoxicity against human H9 cells after 3 days
[PMID: 8158164]
HepG2 IC50
> 400 μM
Compound: 34
Cytotoxicity against human HepG2 cells by MTT assay
Cytotoxicity against human HepG2 cells by MTT assay
[PMID: 21726077]
HT-22 EC50
2.8 μM
Compound: fisetin
Neuroprotective effect against iodoacetic acid-induced ischemia in mouse HT22 cells treated simultaneously with iodoacetic acid for 2 hrs followed by incubation in fresh medium containing drug alone for 20 hrs measured after 4 hrs by MTT assay
Neuroprotective effect against iodoacetic acid-induced ischemia in mouse HT22 cells treated simultaneously with iodoacetic acid for 2 hrs followed by incubation in fresh medium containing drug alone for 20 hrs measured after 4 hrs by MTT assay
[PMID: 19537799]
HT-22 EC50
3 μM
Compound: Fisetin
Neuroprotective activity in mouse HT22 cells assessed as reduction in IAA-induced ischemia after 24 hrs by MTT assay
Neuroprotective activity in mouse HT22 cells assessed as reduction in IAA-induced ischemia after 24 hrs by MTT assay
[PMID: 22192055]
Huh-7 CC50
> 50 μM
Compound: 39
Cytotoxicity against human Huh7.5.1 cells by MTT assay
Cytotoxicity against human Huh7.5.1 cells by MTT assay
[PMID: 22445328]
L6 IC50
> 30 μg/mL
Compound: 20
Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by photometric method
Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by photometric method
10.1039/C5MD00481K
L6 IC50
38.5 μg/mL
Compound: 20
Cytotoxicity against rat L6 cells after 72 hrs by alamar blue staining based fluorescence analysis
Cytotoxicity against rat L6 cells after 72 hrs by alamar blue staining based fluorescence analysis
10.1039/C5MD00481K
LNCaP IC50
34.1 μM
Compound: Fisetin
Antiproliferative activity against human LNCAP cells after 3 days by WST-1 assay
Antiproliferative activity against human LNCAP cells after 3 days by WST-1 assay
[PMID: 30165345]
LNCaP IC50
34.1 μM
Compound: 2
Antiproliferative activity against human LNCAP cells after 3 days by WST-1 assay
Antiproliferative activity against human LNCAP cells after 3 days by WST-1 assay
[PMID: 28760528]
MCF-10A IC50
2.44 μM
Compound: Fisetin
Cytotoxicity against human MCF10A cells assessed as decrease in cell viability after 3 days by WST-1 assay
Cytotoxicity against human MCF10A cells assessed as decrease in cell viability after 3 days by WST-1 assay
[PMID: 30165345]
Monocyte IC50
3.7 μM
Compound: fisetin
Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs
[PMID: 8882428]
PC-3 IC50
> 50 μM
Compound: Fisetin
Antiproliferative activity against human PC3 cells after 3 days by WST-1 assay
Antiproliferative activity against human PC3 cells after 3 days by WST-1 assay
[PMID: 30165345]
PC-3 IC50
> 50 μM
Compound: 2
Antiproliferative activity against human PC3 cells after 3 days by WST-1 assay
Antiproliferative activity against human PC3 cells after 3 days by WST-1 assay
[PMID: 28760528]
PWR-1E IC50
5.39 μM
Compound: Fisetin
Cytotoxicity against human PWR-1E cells assessed as decrease in cell viability after 3 days by WST-1 assay
Cytotoxicity against human PWR-1E cells assessed as decrease in cell viability after 3 days by WST-1 assay
[PMID: 30165345]
RD EC50
84.48 μM
Compound: Fisetin
Antiviral activity against EV71 infected in human RD cells assessed as reduction in viral plaque formation after 1 hr by crystal violet staining method
Antiviral activity against EV71 infected in human RD cells assessed as reduction in viral plaque formation after 1 hr by crystal violet staining method
[PMID: 27776325]
THP-1 IC50
30 μM
Compound: Fisetin
Cytotoxicity against human THP1 cells after 24 hrs by MTS assay
Cytotoxicity against human THP1 cells after 24 hrs by MTS assay
[PMID: 19879672]
Vero IC50
55 μg/mL
Compound: fisetin
Antiviral activity against dengue virus 2 infected in african green monkey Vero cells assessed as reduction in viral replication dosed after adsorption with 200 FFU of virus for 1 hour by foci forming unit reduction assay
Antiviral activity against dengue virus 2 infected in african green monkey Vero cells assessed as reduction in viral replication dosed after adsorption with 200 FFU of virus for 1 hour by foci forming unit reduction assay
[PMID: 25564380]
體外研究
(In Vitro)

Fisetin 抑制脂質(zhì)積累并抑制 3T3-L1 細(xì)胞中 PPARγ 的表達(dá)。Fisetin 抑制前脂肪細(xì)胞分化的早期階段，并誘導(dǎo) Sirt1 的表達(dá)。Fisetin 促進(jìn) Sirt1 介導(dǎo)的 PPARγ 和 FoxO1 去乙酰化，增強(qiáng) Sirt1 與 PPARγ 啟動子的結(jié)合，從而抑制 PPARγ 轉(zhuǎn)錄活性，從而抑制脂肪形成[1]。
Fisetin 結(jié)合微管蛋白并穩(wěn)定微管，其結(jié)合特性遠(yuǎn)優(yōu)于紫杉醇。Fisetin 處理人前列腺癌細(xì)胞導(dǎo)致微管相關(guān)蛋白 (MAP)-2 和-4 的強(qiáng)烈上調(diào)。Fisetin 顯著抑制 PCa 細(xì)胞增殖、遷移和侵襲。Nudc 是一種與調(diào)節(jié)微管動力學(xué)的微管運動動力蛋白/動力蛋白復(fù)合物相關(guān)的蛋白質(zhì)，被 Fisetin 處理抑制[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

對暴露于 UVB 的小鼠進(jìn)行 Fisetin 處理可減少增生并減少炎癥細(xì)胞的浸潤。Fisetin 處理還可以降低炎癥介質(zhì)，例如 COX-2、PGE2 及其受體 (EP1-EP4) 和 MPO 活性。此外，F(xiàn)isetin 可降低暴露于 UVB 的皮膚中炎性細(xì)胞因子 TNFα、IL-1β 和 IL-6 的水平。Fisetin 處理還可以減少細(xì)胞增殖標(biāo)志物以及 DNA 損傷，p53 和 p21 蛋白的表達(dá)增加就證明了這一點[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

286.24

Formula

C15H10O6
CAS 號
性狀

固體

顏色

Light yellow to yellow

中文名稱

漆黃素；非瑟酮；紫鉚素；非瑟素；漆樹黃酮
結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細(xì)胞實驗： 

DMSO 中的溶解度 : 25 mg/mL (87.34 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.4936 mL 17.4679 mL 34.9357 mL
5 mM 0.6987 mL 3.4936 mL 6.9871 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (7.27 mM); 澄清溶液

    此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (7.27 mM); 澄清溶液

    此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

  • 方案 一

    請依序添加每種溶劑： 45% PEG300    5% Tween-80    50% Saline

    Solubility: 10 mg/mL (34.94 mM); 懸濁液; 超聲助溶

  • 方案 二

    請依序添加每種溶劑： 0.5% CMC/saline water

    Solubility: 25 mg/mL (87.34 mM); 懸濁液; 超聲助溶

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.99%

參考文獻(xiàn)
Cell Assay
[1]

3T3-L1 cells are transfected with reporter vector, and expression plasmids using TransIT-LT1. After 24 h, media is replaced with viral supernatant. After 15-18 h of infection, media is replaced with DMI, 0.1 μM troglitazone and Fisetin (50 μM). Cells are lysed using cell culture lysis buffer two days after addition of differentiation stimulus. Luciferase activity is determined using an analytical luminescence luminometer[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[3]

Mice: The mice are divided into six groups of eight animals each. The mice in the first group are topically treated with 0.2 mL acetone and used as vehicle control. The mice in the second and third groups receive topical treatment of Fisetin 250 nmol/0.2 mL acetone/mouse and 500 nmol/0.2 mL acetone/mouse respectively on their dorsal skin and serves as agent controls. The mice in the fourth, fifth and sixth groups are exposed to UVB. The mice in the fourth group receive a topical application of 0.2 mL acetone after 15 min of UVB exposure designated as vehicle control. The mice in the fifth and sixth groups are treated with topical application of Fisetin 250 nmol/0.2 mL acetone/mouse and 500 nmol/0.2 mL acetone/mouse respectively on to their dorsal skin after 15 min of UVB exposure[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4936 mL 17.4679 mL 34.9357 mL 87.3393 mL
5 mM 0.6987 mL 3.4936 mL 6.9871 mL 17.4679 mL
10 mM 0.3494 mL 1.7468 mL 3.4936 mL 8.7339 mL
15 mM 0.2329 mL 1.1645 mL 2.3290 mL 5.8226 mL
20 mM 0.1747 mL 0.8734 mL 1.7468 mL 4.3670 mL
25 mM 0.1397 mL 0.6987 mL 1.3974 mL 3.4936 mL
30 mM 0.1165 mL 0.5823 mL 1.1645 mL 2.9113 mL
40 mM 0.0873 mL 0.4367 mL 0.8734 mL 2.1835 mL
50 mM 0.0699 mL 0.3494 mL 0.6987 mL 1.7468 mL
60 mM 0.0582 mL 0.2911 mL 0.5823 mL 1.4557 mL
80 mM 0.0437 mL 0.2183 mL 0.4367 mL 1.0917 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Fisetin
目錄號：
HY-N0182
需求量：