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  2. Cell Cycle/DNA Damage Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease NF-κB Immunology/Inflammation Anti-infection
  3. Dengue virus PPAR Reactive Oxygen Species Influenza Virus Endogenous Metabolite
  4. Naringenin

Naringenin  (Synonyms: 柚皮素)

目錄號: HY-N0100 純度: 99.27%
COA 產(chǎn)品使用指南 技術(shù)支持

Naringenin 是柑橘中主要的黃烷酮; 顯示出強烈的抗炎和抗氧化活性。Naringenin 具有抗登革熱病毒 (DENV) 活性。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Naringenin Chemical Structure

Naringenin Chemical Structure

CAS No. : 480-41-1

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規(guī)格 價格 是否有貨 數(shù)量
5 mg ￥500
In-stock
10 mg ￥600
In-stock
50 mg ￥960
In-stock
100 mg ￥1152
In-stock
200 mg   詢價  
500 mg   詢價  

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Customer Review

Other Forms of Naringenin:

    Naringenin purchased from MCE. Usage Cited in: J Funct Foods. 2020 Jun.

    Naringenin prevents the neuroinflammation. Representative Western blot bands of TNF-α, IL-1β, IL-6, IL-10, and iNOS.

    Naringenin purchased from MCE. Usage Cited in: J Funct Foods. 2020 Jun.

    Naringenin decreases the number of Iba-1-positive microglia and fully-activated microglia. Schematic diagram of the selected region of brain section for immunochemistry and Representative Iba-1 staining.

    查看 PPAR 亞型特異性產(chǎn)品:

    • 生物活性

    • 實驗參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Naringenin is the predominant flavanone in Citrus reticulata Blanco; displays strong anti-inflammatory and antioxidant activities. Naringenin has anti-dengue virus (DENV) activity.

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    A549 IC50
    > 100 μM
    Compound: 4
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    Cytotoxicity against human A549 cells after 72 hrs by MTT assay
    		[PMID: 18440233]
    B16-BL6 IC50
    > 100 μM
    Compound: 4
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay
    		[PMID: 18440233]
    BJ EC50
    > 10 μM
    Compound: 2
    Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay
    Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay
    		[PMID: 20192247]
    C8166 CC50
    141.6 μg/mL
    Compound: 6
    Cytotoxicity against human C8166 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay
    Cytotoxicity against human C8166 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay
    		[PMID: 24794743]
    C8166 EC50
    9.47 μg/mL
    Compound: 6
    Antiviral activity against HIV-1 3B infected in human C8166 cells assessed as inhibition of virus-induced cytopathogenicity by measuring syncytial cell number after 3 days by inverted microscopic analysis
    Antiviral activity against HIV-1 3B infected in human C8166 cells assessed as inhibition of virus-induced cytopathogenicity by measuring syncytial cell number after 3 days by inverted microscopic analysis
    		[PMID: 24794743]
    CCRF-CEM EC50
    > 10 μM
    Compound: 2
    Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay
    Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay
    		[PMID: 20192247]
    H9 IC50
    294 μM
    Compound: 19
    Cytotoxicity against human H9 cells after 3 days
    Cytotoxicity against human H9 cells after 3 days
    		[PMID: 8158164]
    H9 EC50
    92 μM
    Compound: 19
    Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay
    Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay
    		[PMID: 8158164]
    HeLa EC50
    > 10 μM
    Compound: 2
    Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay
    Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay
    		[PMID: 20192247]
    HeLa IC50
    > 100 μM
    Compound: 4
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    Cytotoxicity against human HeLa cells after 72 hrs by MTT assay
    		[PMID: 18440233]
    HL-60 IC50
    413.7 μM
    Compound: Naringenin
    Cytotoxicity against human HL60 cells after 46 hrs by MTT assay
    Cytotoxicity against human HL60 cells after 46 hrs by MTT assay
    		[PMID: 23756061]
    HT-1080 IC50
    > 100 μM
    Compound: 4
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay
    		[PMID: 18440233]
    Jurkat IC50
    41.6 μM
    Compound: Naringenin
    Inhibition of chymotrypsin-like activity of human 26S proteasome in human Jurkat cells assessed as decrease in AMC hydrolysis using Z-Gly-Gly-Leu-AMC as substrate after 24 hrs by fluorescence based method
    Inhibition of chymotrypsin-like activity of human 26S proteasome in human Jurkat cells assessed as decrease in AMC hydrolysis using Z-Gly-Gly-Leu-AMC as substrate after 24 hrs by fluorescence based method
    		[PMID: 30776692]
    Jurkat IC50
    48.8 μM
    Compound: Naringenin
    Inhibition of chymotrypsin-like activity of purified human 20S proteasome expressed in human Jurkat cells assessed as decrease in AMC hydrolysis using Suc-Leu-Leu-Val-Tyr-AMC as substrate incubated for 2 hrs by fluorescence based method
    Inhibition of chymotrypsin-like activity of purified human 20S proteasome expressed in human Jurkat cells assessed as decrease in AMC hydrolysis using Suc-Leu-Leu-Val-Tyr-AMC as substrate incubated for 2 hrs by fluorescence based method
    		[PMID: 30776692]
    L5178Y IC50
    ≥ 11.3 μM
    Compound: 1
    Cytotoxicity against parental mouse L5178Y cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay
    Cytotoxicity against parental mouse L5178Y cells assessed as inhibition of cell growth incubated for 24 hrs by MTT assay
    		[PMID: 33038666]
    L5178Y IC50
    ≥ 11.3 μM
    Compound: 1
    Cytotoxicity against multi-drug resistant mouse L5178Y cells transfected with ABCB1 gene assessed as inhibition of cell growth incubated for 24 hrs by MTT assay
    Cytotoxicity against multi-drug resistant mouse L5178Y cells transfected with ABCB1 gene assessed as inhibition of cell growth incubated for 24 hrs by MTT assay
    		[PMID: 33038666]
    Lewis lung carcinoma cell line IC50
    > 100 μM
    Compound: 4
    Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
    Cytotoxicity against mouse LLC cells after 72 hrs by MTT assay
    		[PMID: 18440233]
    MCF7 EC50
    > 10 μM
    Compound: 2
    Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay
    Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay
    		[PMID: 20192247]
    MCF7 IC50
    0.3 μM
    Compound: Naringenin
    Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 6 days by SRB assay
    Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 6 days by SRB assay
    		[PMID: 33257172]
    MCF7 IC50
    5.1 μM
    Compound: Naringenin
    Inhibition of aromatase in human MCF7 cells using [1beta-3H]androstenedione as substrate measured after 1 hr by liquid scintillation counting method
    Inhibition of aromatase in human MCF7 cells using [1beta-3H]androstenedione as substrate measured after 1 hr by liquid scintillation counting method
    		[PMID: 27155469]
    NALM-6 IC50
    426.3 μM
    Compound: Naringenin
    Cytotoxicity against human NALM6 cells after 46 hrs by MTT assay
    Cytotoxicity against human NALM6 cells after 46 hrs by MTT assay
    		[PMID: 23756061]
    RAW264.7 IC50
    60 μM
    Compound: 11
    Inhibition of LPS/INF-gamma-stimulated nitric oxide production in mouse RAW264.7 cells measured after 16 hrs
    Inhibition of LPS/INF-gamma-stimulated nitric oxide production in mouse RAW264.7 cells measured after 16 hrs
    		[PMID: 27955927]
    RPMI-8226 EC50
    > 10 μM
    Compound: 2
    Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay
    Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay
    		[PMID: 20192247]
    THP-1 IC50
    > 10 μM
    Compound: 25
    Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by WST assay
    Cytotoxicity against human THP1 cells assessed as cell viability after 24 hrs by WST assay
    		[PMID: 25735399]
    THP-1 EC50
    > 10 μM
    Compound: 2
    Cytotoxicity against human THP1 cells after 72 hrs by erythosin B staining method
    Cytotoxicity against human THP1 cells after 72 hrs by erythosin B staining method
    		[PMID: 20192247]
    U-266 EC50
    > 10 μM
    Compound: 2
    Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay
    Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay
    		[PMID: 20192247]
    WM-115 IC50
    524.8 μM
    Compound: Naringenin
    Cytotoxicity against human WM115 cells after 46 hrs by MTT assay
    Cytotoxicity against human WM115 cells after 46 hrs by MTT assay
    		[PMID: 23756061]
    體外研究
    (In Vitro)

    Naringenin is shown to inhibit the proliferation of HepG2 cells resulted partly from an accumulation of cells in the G0/G1 and G2/M phase of the cell cycle. Naringenin has been shown to induce apoptosis as evidenced by nuclei damage and increased proportion of apoptotic cells. Naringenin triggers the mitochondrial-mediated apoptosis pathway as shown by an increased ratio of Bax/Bcl-2, subsequent release of cytochrome C, and sequential activation of caspase-3[1]. Naringenin exposure significantly reduces the cell viability of A431 cells with a concomitant increase in nuclear condensation and DNA fragmentation in a dose dependent manner. Cell cycle study shows that naringenin induced cell cycle arrest in G0/G1 phase of cell cycle and caspase-3 analysis reveal a dose dependent increment in caspase-3 activity which leads to cell apoptosis[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    Naringenin supplementation causes a significant reduction in the amount of total triglyceride and cholesterol in plasma and liver. In addition, naringenin supplementation lowers adiposity and triglyceride contents in parametrial adipose tissue. Naringenin-fed animals show a significant increase in PPARα protein expression in the liver. The expression of CPT-1 and UCP2, known to be regulated by PPARα, is markedly enhanced by naringenin treatment[3]. Naringenin increases hepatic fatty acid oxidation through a PPARγ coactivator 1α/PPARα-mediated transcription program. It prevents sterol regulatory element-binding protein 1c–mediated lipogenesis in both liver and muscle by reducing fasting hyperinsulinemia. Naringenin decreases hepatic cholesterol and cholesterol ester synthesis[4]. Naringenin inhibits TNF-α-induced VSMC proliferation and migration in a dose-dependent manner. Mechanistic study demonstrates that naringenin prevents ERK/MAPK and Akt phosphorylation while left p38 MAPK and JNK unchanged. Naringenin also blocks the increase of ROS generation induced by TNF-α[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    272.25

    Formula

    C15H12O5
    CAS 號
    性狀

    固體

    顏色

    White to light yellow

    中文名稱

    柚皮素； 柑桔素
    結(jié)構(gòu)分類
    初始來源
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性數(shù)據(jù)
    細(xì)胞實驗： 

    Methanol 中的溶解度 : ≥ 100 mg/mL (367.31 mM)

    DMSO 中的溶解度 : 66.67 mg/mL (244.89 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 3.6731 mL 18.3655 mL 36.7309 mL
    5 mM 0.7346 mL 3.6731 mL 7.3462 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 3 mg/mL (11.02 mM); 澄清溶液

      此方案可獲得 ≥ 3 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 30.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 3 mg/mL (11.02 mM); 澄清溶液

      此方案可獲得 ≥ 3 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 30.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

    以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請依序添加每種溶劑： 50% PEG300    50% Saline

      Solubility: 33.33 mg/mL (122.42 mM); 澄清溶液; 超聲助溶

    • 方案 二

      請依序添加每種溶劑： 0.5% CMC/saline water

      Solubility: 30.3 mg/mL (111.29 mM); 懸濁液; 超聲助溶

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    請輸入您的動物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計算結(jié)果
    工作液所需濃度 : mg/mL
    儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。
    請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.27%

    參考文獻(xiàn)
    Cell Assay
    [1]

    Naringenin is dissolved in DMSO and diluted in cell culture medium. The cells are rinsed with PBS and grown in a medium containing various concentrations of naringenin (50, 100, 150, 200, 250, 300 μM). The solvent DMSO treated cells are served as control. After 24 hrs of treatment, the medium is removed and replaced by another medium containing MTT. Cell viability is measured using the MTT assay[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3][4]

    Rats: Semi-purified, powdered diets are prepared for concentrations of naringenin: 0, 0.003, 0.006, and 0.012% of diet. After 7 days of acclimatization, rats are assigned to one of four groups, with six animals per group, and fed semi-purified experimental diets for 6 weeks. The experimental diets contain 16% fat, 45.5% sucrose, and different naringenin concentration (0, 0.003, 0.006, or 0.012%) (Table 1). Rats have ad libitum access to food and water during the study period. Food intake and body weight are measured throughout the experiment[3].

    Mouse: Eight- to 12-week-old mice are fed ad libitum a rodent standard diet or a high-fat diet containing 42% of calories from fat plus cholesterol (0.05% wt/wt). Naringenin is added to the Western diet at 1 or 3% (wt/wt). Ldlr?/? mice are fed for 4 weeks and C57BL/6J mice for 30 weeks. Food intake is measured daily, and body weight is measured biweekly. Mice are fasted for 6 h before intervention[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻(xiàn)

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO / Methanol 1 mM 3.6731 mL 18.3655 mL 36.7309 mL 91.8274 mL
    5 mM 0.7346 mL 3.6731 mL 7.3462 mL 18.3655 mL
    10 mM 0.3673 mL 1.8365 mL 3.6731 mL 9.1827 mL
    15 mM 0.2449 mL 1.2244 mL 2.4487 mL 6.1218 mL
    20 mM 0.1837 mL 0.9183 mL 1.8365 mL 4.5914 mL
    25 mM 0.1469 mL 0.7346 mL 1.4692 mL 3.6731 mL
    30 mM 0.1224 mL 0.6122 mL 1.2244 mL 3.0609 mL
    40 mM 0.0918 mL 0.4591 mL 0.9183 mL 2.2957 mL
    50 mM 0.0735 mL 0.3673 mL 0.7346 mL 1.8365 mL
    60 mM 0.0612 mL 0.3061 mL 0.6122 mL 1.5305 mL
    80 mM 0.0459 mL 0.2296 mL 0.4591 mL 1.1478 mL
    100 mM 0.0367 mL 0.1837 mL 0.3673 mL 0.9183 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Naringenin
    目錄號：
    HY-N0100
    需求量：