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NEP (VDP-green (NEP)) 是一種基于分子內(nèi)電荷轉(zhuǎn)移 (ICT) 機(jī)制的感應(yīng)熒光探針，用于感應(yīng)含鄰二硫醇的蛋白質(zhì) (VDPs)。NEP 在活細(xì)胞和動(dòng)物體內(nèi)對(duì) VDPs 表現(xiàn)出高選擇性，并顯示強(qiáng)綠色熒光信號(hào) (λex/λem=430/535 nm)。NEP 具有用于帕金森氏癥研究的潛力。

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NEP Chemical Structure

NEP Chemical Structure

CAS No. : 2414276-32-5

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  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

NEP (VDP-green (NEP)) is a turn-on fluorescent probe based on the intramolecular charge transfer (ICT) mechanism for sensing vicinal dithiol-containing proteins (VDPs). NEP exhibits high selectivity toward VDPs in live cells and in vivo and displays a strong green fluorescence signal (λex/λem=430/535 nm). NEP has the potential for parkinsonism[1].

體外研究
(In Vitro)

NEP (1-30 μM; 6 hours) has no or little cytotoxicity in HepG2 and PC12 cells[1].
NEP (10 μM; 4 hours) causes the fluorescence intensity to decrease gradually in PC12 cells pretreated with 6-OHDA (0, 50, 100, and 200 μM; for 30 min)[1].
NEP contains a dithiarsolane moiety (five-membered As-S ring) as the receptor of VDPs. In the presence of VDPs, NEP displays a strong green fluorescence signal produced by the cyclic dithiarsolane cleavage and subsequent intramolecular cyclization to liberate the fluorophore. NEP maintains a reliable fluorescence response within the range of pH 7-10[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

NEP (10 μM; for 4 h) causes the obvious green signal in zebrafishes (4 day old)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

654.63

Formula

C29H31AsN4O5S2
CAS 號(hào)
Emission (Em)

535
Excitation (Ex)

430
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
純度 & 產(chǎn)品資料
參考文獻(xiàn)

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
NEP
目錄號(hào)：
HY-D1259
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