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  1. Others
  2. Fluorescent Dye
  3. Calcein

Calcein 是一種熒光染料和自滅探針,可以作為脂囊泡滲漏的指示劑,也可作 EDTA 鈣離子滴定和熒光測(cè)定鈣離子的絡(luò)合指示劑。 Calcein 也可以作為模型藥物,用于評(píng)估藥物遞送系統(tǒng)的效率和生物利用度。

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Calcein Chemical Structure

Calcein Chemical Structure

CAS No. : 1461-15-0

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Customer Review

Other Forms of Calcein:

    Calcein purchased from MCE. Usage Cited in: Drug Des Devel Ther. 2022, 16: 3929-3946.

    Calcein and tetracycline labeling of mice femur at 8 weeks. The red arrow indicates the distance between calcein and tetracycline fluorescence labeling.
    • 生物活性

    • 實(shí)驗(yàn)參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems[1][2].

    體外研究
    (In Vitro)

    Calcein 在培養(yǎng)于體腔液或 ISO-EDTA 中的體腔細(xì)胞內(nèi)累積。與對(duì)照組相比,培養(yǎng)于體腔液中的體腔細(xì)胞表現(xiàn)出 calcein 熒光強(qiáng)度的增加。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    622.53

    Formula

    C30H26N2O13

    CAS 號(hào)
    性狀

    固體

    顏色

    Yellow to orange

    Emission (Em)

    515

    Excitation (Ex)

    489

    中文名稱

    鈣黃綠素

    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.

    儲(chǔ)存方式

    RT, stored under nitrogen

    In solvent -80°C 2 years
    -20°C 1 year
    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn): 

    1M NaOH 中的溶解度 : 50 mg/mL (80.32 mM; 超聲助溶)

    H2O 中的溶解度 : 5.65 mg/mL (9.08 mM; ultrasonic and warming and adjust pH to 8 with 1 M NaOH and heat to 60°C)

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 1.6063 mL 8.0317 mL 16.0635 mL
    5 mM 0.3213 mL 1.6063 mL 3.2127 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液;一旦配成溶液,請(qǐng)分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效
    儲(chǔ)備液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí),請(qǐng)?jiān)?年內(nèi)使用,-20°C儲(chǔ)存時(shí),請(qǐng)?jiān)?年內(nèi)使用。

    * 備注:如您選擇水作為儲(chǔ)備液,請(qǐng)稀釋至工作液后,再用 0.22 μm 的濾膜過濾除菌后使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn):

    以下溶解方案,請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配,在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比; 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請(qǐng)依序添加每種溶劑: 2% NaHCO3 in ddH2O

      Solubility: 20 mg/mL (32.13 mM); 澄清溶液; Need ultrasonic and adjust pH to 6 with NaHCO3

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息:

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    由于實(shí)驗(yàn)過程有損耗,建議您多配一只動(dòng)物的量
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測(cè)溶解度,如有需要,請(qǐng)與 MCE 中國技術(shù)支持聯(lián)系。
    純度 & 產(chǎn)品資料

    染色示例
    參考文獻(xiàn)
    Cell Assay
    [1]

    Coelomocytes suspensions (ISO and CF) are separately incubated with calcein-AM (excitation and emission wavelength: 496 nm and 516 nm, respectively) at a final concentration of 200 nM, during 30 min, at 26°C. Calcein-AM is a nonfluorescent ABC transporter substrate whose intracellular accumulation is inversely proportional to ABC transporter activity. Intracellular esterases convert calcein-AM into the fluorescent dye calcein, which is not an ABC transporter substrate, thereby accumulating the dye inside the cell. Therefore, a high fluorescence signal indicates low ABC transporter activity whereas a low fluorescence signal indicates high activity. The fluorescence of samples is measured by flow cytometer. The experiment is repeated six times in duplicates.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液;一旦配成溶液,請(qǐng)分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí),請(qǐng)?jiān)?年內(nèi)使用,-20°C儲(chǔ)存時(shí),請(qǐng)?jiān)?年內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O / 1M NaOH 1 mM 1.6063 mL 8.0317 mL 16.0635 mL 40.1587 mL
    5 mM 0.3213 mL 1.6063 mL 3.2127 mL 8.0317 mL
    1M NaOH 10 mM 0.1606 mL 0.8032 mL 1.6063 mL 4.0159 mL
    15 mM 0.1071 mL 0.5354 mL 1.0709 mL 2.6772 mL
    20 mM 0.0803 mL 0.4016 mL 0.8032 mL 2.0079 mL
    25 mM 0.0643 mL 0.3213 mL 0.6425 mL 1.6063 mL
    30 mM 0.0535 mL 0.2677 mL 0.5354 mL 1.3386 mL
    40 mM 0.0402 mL 0.2008 mL 0.4016 mL 1.0040 mL
    50 mM 0.0321 mL 0.1606 mL 0.3213 mL 0.8032 mL
    60 mM 0.0268 mL 0.1339 mL 0.2677 mL 0.6693 mL
    80 mM 0.0201 mL 0.1004 mL 0.2008 mL 0.5020 mL

    * 備注:如您選擇水作為儲(chǔ)備液,請(qǐng)稀釋至工作液后,再用 0.22 μm 的濾膜過濾除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Inquiry Information

    產(chǎn)品名稱:
    Calcein
    目錄號(hào):
    HY-D0040
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