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  2. 誘導(dǎo)疾病模型產(chǎn)品 Metabolic Enzyme/Protease Autophagy Apoptosis
  3. 神經(jīng)系統(tǒng)疾病模型 Mitochondrial Metabolism Autophagy Apoptosis
  4. 帕金森綜合癥模型
  5. Rotenone

Rotenone 是線粒體電子傳遞鏈復(fù)合物 I 抑制劑。 Rotenone 通過促進(jìn)線粒體活性氧的產(chǎn)生來誘導(dǎo)細(xì)胞凋亡。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Rotenone Chemical Structure

Rotenone Chemical Structure

CAS No. : 83-79-4

1.  客戶無需承擔(dān)相應(yīng)的運輸費用。

2.  同一機(jī)構(gòu)（單位）同一產(chǎn)品試用裝僅限申領(lǐng)一次，同一機(jī)構(gòu)（單位）一年內(nèi)

     可免費申領(lǐng)三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥500
In-stock
500 mg ￥400
In-stock
1 g ￥550
In-stock
5 g ￥1100
In-stock
10 g   詢價  
50 g   詢價  

* Please select Quantity before adding items.

Other Forms of Rotenone:

MCE 顧客使用本產(chǎn)品發(fā)表的 86 篇科研文獻(xiàn)

WB

    Rotenone purchased from MCE. Usage Cited in: Mediat Inflamm. 2020 Aug 17;2020:3934769.  [Abstract]

    Western blot analysis of NLRP3, IL-1β, IL-18, and caspase-1 p10 expression in renal tubular epithelial cells stimulated with Rotenone. Rotenone induces NLRP3 inflammasome activation.

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Rotenone is a mitochondrial electron transport chain complex I inhibitor. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    697 IC50
    0.3 μM
    Compound: 8
    Cytotoxicity against human 697 cells after 48 hrs by MTS assay
    Cytotoxicity against human 697 cells after 48 hrs by MTS assay
    		[PMID: 23895019]
    697 IC50
    0.3 μM
    Compound: 8
    Cytotoxicity against human 697 cells after 72 hrs by MTS assay
    Cytotoxicity against human 697 cells after 72 hrs by MTS assay
    		[PMID: 23895019]
    A549 IC50
    0.04 μg/mL
    Compound: 3
    Cytotoxicity against human A549 cells after 3 days by SRB assay
    Cytotoxicity against human A549 cells after 3 days by SRB assay
    		[PMID: 16562843]
    A549 IC50
    0.901 μg/mL
    Compound: 1
    Cytotoxicity against human A549 cells by SRB assay
    Cytotoxicity against human A549 cells by SRB assay
    		[PMID: 22204911]
    A549 ED50
    26 μM
    Compound: rotenone
    Antitumor activity against human A549 cells after 24 hrs by MTT assay
    Antitumor activity against human A549 cells after 24 hrs by MTT assay
    		[PMID: 17482824]
    BC1 cell line ED50
    0.039 μg/mL
    Compound: 1
    Cytotoxicity against human BC1 cells after 72 hrs
    Cytotoxicity against human BC1 cells after 72 hrs
    		[PMID: 2614425]
    Col2 ED50
    0.15 μg/mL
    Compound: 1
    Cytotoxicity against human Col2 cells after 72 hrs
    Cytotoxicity against human Col2 cells after 72 hrs
    		[PMID: 2614425]
    DU-145 IC50
    0.465 μg/mL
    Compound: 1
    Cytotoxicity against human DU145 cells by SRB assay
    Cytotoxicity against human DU145 cells by SRB assay
    		[PMID: 22204911]
    HCT-116 IC50
    0.045 ug
    Compound: 5
    Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting
    Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting
    		[PMID: 21452840]
    HeLa IC50
    78 μM
    Compound: Rotenone
    Cytotoxicity against human HeLa cells after 24 hrs by luminometry
    Cytotoxicity against human HeLa cells after 24 hrs by luminometry
    		[PMID: 21741837]
    HepG2 IC50
    1.52 μM
    Compound: Rotenone
    Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay
    		[PMID: 30613327]
    HepG2 ED50
    15 μM
    Compound: rotenone
    Antitumor activity against human HepG2 cells after 24 hrs by MTT assay
    Antitumor activity against human HepG2 cells after 24 hrs by MTT assay
    		[PMID: 17482824]
    HT-1080 ED50
    0.047 μg/mL
    Compound: 1
    Cytotoxicity against human HT1080 cells after 48 hrs
    Cytotoxicity against human HT1080 cells after 48 hrs
    		[PMID: 2614425]
    HT-29 IC50
    0.3 μM
    Compound: 8
    Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay
    Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay
    		[PMID: 23895019]
    HT-29 ED50
    202 μM
    Compound: rotenone
    Antitumor activity against human HT29 cells after 24 hrs by MTT assay
    Antitumor activity against human HT29 cells after 24 hrs by MTT assay
    		[PMID: 17482824]
    KB ED50
    0.067 μg/mL
    Compound: 1
    Cytotoxicity against human KB cells after 72 hrs
    Cytotoxicity against human KB cells after 72 hrs
    		[PMID: 2614425]
    KB IC50
    0.513 μg/mL
    Compound: 1
    Cytotoxicity against human KB cells by SRB assay
    Cytotoxicity against human KB cells by SRB assay
    		[PMID: 22204911]
    L5178Y IC50
    0.3 μM
    Compound: 30
    Cytotoxicity against mouse L5178Y cells by MTT assay
    Cytotoxicity against mouse L5178Y cells by MTT assay
    		[PMID: 28075580]
    MCF7 IC50
    0.008 μg/mL
    Compound: 3
    Cytotoxicity against human MCF7 cells after 3 days by SRB assay
    Cytotoxicity against human MCF7 cells after 3 days by SRB assay
    		[PMID: 16562843]
    MCF7 ED50
    161 μM
    Compound: rotenone
    Antitumor activity against human MCF7 cells after 24 hrs by MTT assay
    Antitumor activity against human MCF7 cells after 24 hrs by MTT assay
    		[PMID: 17482824]
    MES-SA ED50
    147 μM
    Compound: rotenone
    Antitumor activity against human MES-SA cells after 24 hrs by MTT assay
    Antitumor activity against human MES-SA cells after 24 hrs by MTT assay
    		[PMID: 17482824]
    MES-SA/Dx5 ED50
    214 μM
    Compound: rotenone
    Antitumor activity against doxorubucin-resistant human MES-SA/Dx5 cells by MTT assay after 24 hrs
    Antitumor activity against doxorubucin-resistant human MES-SA/Dx5 cells by MTT assay after 24 hrs
    		[PMID: 17482824]
    P388 ED50
    0.005 μg/mL
    Compound: 1
    Cytotoxicity against mouse P388 cells after 48 hrs
    Cytotoxicity against mouse P388 cells after 48 hrs
    		[PMID: 2614425]
    Raji IC50
    1.1 μM
    Compound: 8
    Cytotoxicity against human Raji cells after 72 hrs by MTS assay
    Cytotoxicity against human Raji cells after 72 hrs by MTS assay
    		[PMID: 23895019]
    Raji IC50
    4.2 μM
    Compound: 8
    Cytotoxicity against human Raji cells after 48 hrs by MTS assay
    Cytotoxicity against human Raji cells after 48 hrs by MTS assay
    		[PMID: 23895019]
    SH-SY5Y IC50
    74.1 μM
    Compound: Rotenone
    Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability after 24 hrs by MTT assay
    Cytotoxicity against human SH-SY5Y cells assessed as reduction in cell viability after 24 hrs by MTT assay
    		[PMID: 27689727]
    SK-MEL-2 ED50
    0.092 μg/mL
    Compound: 1
    Cytotoxicity against human SK-MEL-2 cells after 72 hrs
    Cytotoxicity against human SK-MEL-2 cells after 72 hrs
    		[PMID: 2614425]
    U-937 IC50
    0.07 μM
    Compound: 3
    Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay
    		[PMID: 17158054]
    U-937 IC50
    0.2 μM
    Compound: 3
    Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay
    		[PMID: 17158054]
    U-937 IC50
    13 μM
    Compound: 3
    Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
    Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay
    		[PMID: 17158054]
    體外研究
    (In Vitro)

    絲裂原活化蛋白激酶 (MAPK)、Toll 樣受體、Wnt 和 Ras 信號通路密切參與 Rotenone 對 ENS 的影響[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    Rotenone 可用于構(gòu)建帕金森模型。

    誘導(dǎo)帕金森模型[6]
    致病原理
    黑質(zhì)多巴胺能 (DA) 神經(jīng)元細(xì)胞丟失，是帕金森的常見特征。Rotenone 能夠誘導(dǎo) DA 神經(jīng)元細(xì)胞毒性，導(dǎo)致小鼠黑質(zhì)運動缺陷和 DA 神經(jīng)元細(xì)胞的丟失。
    具體造模方法：
    小鼠：雄性 • C57BL/6J 小鼠 • 8 周齡 • 20-25 g
    給藥方式：30 mg/kg, 12 mL/kg • 口服 • 每天一次，連續(xù) 28 天
    Note
    對照組用 0.5% Carboxylmethylcellulose (CMC) 處理。
    造模成功指標(biāo)
    小鼠運動障礙：運動遲緩/運動能力不足。
    相關(guān)產(chǎn)品： /
    拮抗產(chǎn)品： /

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    394.42

    Formula

    C23H22O6
    CAS 號
    性狀

    固體

    顏色

    White to light yellow

    中文名稱

    魚藤酮
    結(jié)構(gòu)分類
    初始來源
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    4°C, stored under nitrogen

    *In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

    溶解性數(shù)據(jù)
    細(xì)胞實驗： 

    DMSO 中的溶解度 : 50 mg/mL (126.77 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 2.5354 mL 12.6768 mL 25.3537 mL
    5 mM 0.5071 mL 2.5354 mL 5.0707 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (stored under nitrogen)。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (6.34 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (6.34 mM); 懸濁液; 超聲助溶

      此方案可獲得 2.5 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

    以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請依序添加每種溶劑： 0.5% CMC-Na/saline water

      Solubility: 25 mg/mL (63.38 mM); 懸濁液; 超聲助溶

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    請輸入您的動物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計算結(jié)果
    工作液所需濃度 : mg/mL
    儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

    *In solvent : -80°C, 1 year; -20°C, 6 months (stored under nitrogen)

    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。
    請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.74%

    參考文獻(xiàn)

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (stored under nitrogen)。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.5354 mL 12.6768 mL 25.3537 mL 63.3842 mL
    5 mM 0.5071 mL 2.5354 mL 5.0707 mL 12.6768 mL
    10 mM 0.2535 mL 1.2677 mL 2.5354 mL 6.3384 mL
    15 mM 0.1690 mL 0.8451 mL 1.6902 mL 4.2256 mL
    20 mM 0.1268 mL 0.6338 mL 1.2677 mL 3.1692 mL
    25 mM 0.1014 mL 0.5071 mL 1.0141 mL 2.5354 mL
    30 mM 0.0845 mL 0.4226 mL 0.8451 mL 2.1128 mL
    40 mM 0.0634 mL 0.3169 mL 0.6338 mL 1.5846 mL
    50 mM 0.0507 mL 0.2535 mL 0.5071 mL 1.2677 mL
    60 mM 0.0423 mL 0.2113 mL 0.4226 mL 1.0564 mL
    80 mM 0.0317 mL 0.1585 mL 0.3169 mL 0.7923 mL
    100 mM 0.0254 mL 0.1268 mL 0.2535 mL 0.6338 mL
    Help & FAQs
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Rotenone
    目錄號：
    HY-B1756
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