Niacin (Synonyms: 煙酸; Nicotinic acid; Vitamin B3)

目錄號: HY-B0143 純度: 99.93%: FAQs
Data Sheet SDS COA 產(chǎn)品使用指南

摩爾計算器

Niacin (Vitamin B3) 是一種具有口服活性的水溶性B3維生素 (B3 vitamin)，是人類必需的營養(yǎng)物質(zhì)。Niacin (Vitamin B3) 在能量代謝、細胞信號級聯(lián)調(diào)節(jié)基因表達和細胞凋亡 (apoptosis) 中起關(guān)鍵作用，也可用于心血管疾病的研究。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務

Niacin Chemical Structure

CAS No. : 59-67-6

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規(guī)格	價格	是否有貨
10 mM * 1 mL in DMSO	￥500	In-stock
1 g	￥450	In-stock
5 g		詢價
10 g		詢價

or 大包裝詢價

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Customer Review

Other Forms of Niacin:

MCE 顧客使用本產(chǎn)品發(fā)表的 8 篇科研文獻

•Glia. 2018 Feb;66(2):256-278. [Abstract]
•Mil Med Res. 2022 Aug 23;9(1):46. [Abstract]
•Mol Cell. 2023 Aug 11;S1097-2765(23)00605-6. [Abstract]
•Gut Microbes. 2023 Jan-Dec;15(1):2186114. [Abstract]
•J Nanobiotechnology. 2022 Mar 9;20(1):120. [Abstract]
•Mol Neurobiol. 2024 Apr 16. [Abstract]

•Commun Biol. 2023 Mar 2;6(1):235. [Abstract]
•bioRxiv. 2023 Mar 29.

: Niacin purchased from MCE. Usage Cited in: Glia. 2018 Feb;66(2):256-278. [Abstract]; Representative images and quantitative analysis showing the effects of Niacin (1 mM, 10 min) and MK-6892 (10 lM, 10 min) on Akt phosphorylation levels in primary cultured microglia.

Niacin 相關(guān)產(chǎn)品

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•同靶點產(chǎn)品:

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生物活性
純度 & 產(chǎn)品資料
參考文獻

生物活性

Niacin (Vitamin B3) is an orally active water-soluble B3 vitamin that is an essential nutrient for humans. Niacin (Vitamin B3) plays a key role in energy metabolism, cell signaling cascades regulating gene expression and apoptosis. Niacin (Vitamin B3) is also used in the study of cardiovascular diseases^[1]^[2].

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

細胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-431	EC₅₀	0.73 μM Compound: Niacin	Activation of GPR109A in human A431 cells assessed as suppression of forskolin-induced cAMP production after 30 mins Activation of GPR109A in human A431 cells assessed as suppression of forskolin-induced cAMP production after 30 mins	[PMID: 26784936]
Adipocyte	IC₅₀	0.07 μM Compound: Niacin	Inhibition of lipolysis in IBMX-stimulated human adipocytes assessed as glycerol release after 5 hrs Inhibition of lipolysis in IBMX-stimulated human adipocytes assessed as glycerol release after 5 hrs	[PMID: 19309152]
Adipocyte	EC₅₀	0.9 μM Compound: NA	Inhibition of isoproterenol-induced lipolysis in human subcutaneous adipocytes after 5 hrs Inhibition of isoproterenol-induced lipolysis in human subcutaneous adipocytes after 5 hrs	[PMID: 17452318]
CCRF-CEM	IC₅₀	> 500 μM Compound: Niacin	Cytotoxicity against human CEM cells assessed as growth inhibition after 72 hrs by coulter counter method Cytotoxicity against human CEM cells assessed as growth inhibition after 72 hrs by coulter counter method	[PMID: 28238612]
CHO	EC₅₀	0.027 μM Compound: Niacin	Agonist activity at GPR109a expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production by HTRF assay Agonist activity at GPR109a expressed in CHO cells assessed as inhibition of forskolin-stimulated cAMP production by HTRF assay	[PMID: 20363624]
CHO	EC₅₀	1 μM Compound: nicotinic acid	Agonist activity at human GPR109A receptor expressed in CHO cells by [35S]GTPgammaS binding assay Agonist activity at human GPR109A receptor expressed in CHO cells by [35S]GTPgammaS binding assay	[PMID: 18752940]
CHO	IC₅₀	1 μM Compound: niacin	Agonist activity at human niacin receptor expressed in CHO cells by GTPgammaS binding assay Agonist activity at human niacin receptor expressed in CHO cells by GTPgammaS binding assay	[PMID: 18477506]
CHO	EC₅₀	51 nM Compound: niacin, Niaspan	Agonist activity at human GPR109a expressed in CHO cells assessed as decrease in forskolin-stimulated cAMP production by HTRF assay Agonist activity at human GPR109a expressed in CHO cells assessed as decrease in forskolin-stimulated cAMP production by HTRF assay	[PMID: 22435740]
CHO-K1	EC₅₀	1 μM Compound: Niacin	Agonist activity at human GPR109A expressed in CHOK1 cells by [35S]GTPgammaS binding assay Agonist activity at human GPR109A expressed in CHOK1 cells by [35S]GTPgammaS binding assay	[PMID: 19309152]
CHO-K1	EC₅₀	1 μM Compound: niacin	Agonist activity at human GPR109A expressed in CHOK1 cells by [35S]GTP-gamma-S binding assay Agonist activity at human GPR109A expressed in CHOK1 cells by [35S]GTP-gamma-S binding assay	[PMID: 17994679]
CHO-K1	EC₅₀	1.4 μM Compound: nicotinic acid	Agonist activity at human GPR109A receptor expressed in CHO-K1 cells by [35S]GTPgammaS guanine nucleotide exchange assay Agonist activity at human GPR109A receptor expressed in CHO-K1 cells by [35S]GTPgammaS guanine nucleotide exchange assay	[PMID: 18760600]
CHO-K1	EC₅₀	47 nM Compound: Nicotinic acid	Agonist activity at HCA2 receptor (unknown origin) expressed in CHOK1 cells assessed as ERK1/2 phosphorylation by ELISA Agonist activity at HCA2 receptor (unknown origin) expressed in CHOK1 cells assessed as ERK1/2 phosphorylation by ELISA	[PMID: 25737085]
CHO-K1	EC₅₀	527 nM Compound: NA	Agonist activity at cloned human GPR109A receptor expressed in CHO-K1 cells by [35S]GTPgammaS binding assay Agonist activity at cloned human GPR109A receptor expressed in CHO-K1 cells by [35S]GTPgammaS binding assay	[PMID: 20184326]
CHO-K1	EC₅₀	580 nM Compound: NA	Activation of GPR109A receptor in CHOK1 cells assessed as ERK1/2 MAP kinase activation by ELISA Activation of GPR109A receptor in CHOK1 cells assessed as ERK1/2 MAP kinase activation by ELISA	[PMID: 17452318]
CHO-K1	IC₅₀	67.3 μM Compound: NA	Agonist activity at GPR109A receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced intracellular cAMP production by Flashplate assay Agonist activity at GPR109A receptor expressed in CHOK1 cells assessed as inhibition of forskolin-induced intracellular cAMP production by Flashplate assay	[PMID: 17452318]
COS-7	EC₅₀	0.78 μM Compound: 3	Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay Agonist activity at human recombinant GPR109a transfected in african green monkey COS-7 cells after 20 mins by [35S]GTPgammaS binding assay	[PMID: 24900524]
HEK293	IC₅₀	> 500 μM Compound: niacin	Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]
HEK-293T	IC₅₀	249 nM Compound: Nicotinic acid	Displacement of [3H]-nicotinic acid from N-flag-tagged human HCA2 receptor expressed in HEK293T cell membranes by scintillation counting analysis Displacement of [3H]-nicotinic acid from N-flag-tagged human HCA2 receptor expressed in HEK293T cell membranes by scintillation counting analysis	[PMID: 25737085]
HEK-293T	EC₅₀	5 μM Compound: Niacin	Agonist activity at GPR109A receptor (unknown origin) expressed in HEK293T cell membranes after 30 mins by [35S]GTPgammaS binding based microbeta scintillation counting analysis Agonist activity at GPR109A receptor (unknown origin) expressed in HEK293T cell membranes after 30 mins by [35S]GTPgammaS binding based microbeta scintillation counting analysis	[PMID: 32199732]
HL-60	CC₅₀	> 200 μM Compound: Niacin	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 28238612]
HSC-2	CC₅₀	> 200 μM Compound: Niacin	Cytotoxicity against human HSC2 cells after 48 hrs by MTT assay Cytotoxicity against human HSC2 cells after 48 hrs by MTT assay	[PMID: 28238612]
HSC-3	CC₅₀	> 200 μM Compound: Niacin	Cytotoxicity against human HSC3 cells after 48 hrs by MTT assay Cytotoxicity against human HSC3 cells after 48 hrs by MTT assay	[PMID: 28238612]
HSC-4	CC₅₀	> 200 μM Compound: Niacin	Cytotoxicity against human HSC4 cells after 48 hrs by MTT assay Cytotoxicity against human HSC4 cells after 48 hrs by MTT assay	[PMID: 28238612]
L1210	IC₅₀	> 500 μM Compound: Niacin	Cytotoxicity against mouse L1210 cells assessed as growth inhibition after 48 hrs by coulter counter method Cytotoxicity against mouse L1210 cells assessed as growth inhibition after 48 hrs by coulter counter method	[PMID: 28238612]

體外研究
(In Vitro)

Niacin（0-900 μM，42小時）顯著提高GSH水平、降低ROS水平，并影響細胞凋亡和脂質(zhì)代謝相關(guān)基因的表達^[1]。
Niacin（0-40 μM，24小時）可在低劑量下抑制癌癥侵襲活性，但對增殖沒有影響^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	Cumulus cells and oocytes of prepubertal gilts
Concentration:	600 μM
Incubation Time:	42 hours
Result:	Up-regulated the relative expression of the anti-apoptotic gene BCL2 and lipid metabolism gene ACACA while down-regulated the pro-apoptotic gene BAX.

Cell Proliferation Assay^[2]

Cell Line:	Rat ascites hepatoma cell line of AH109A
Concentration:	0-40 μM
Incubation Time:	24 hours
Result:	Had no effect on the proliferation of AH109A cells but suppressed cell invasion from 2.5 μM to 40 μM.

體內(nèi)研究
(In Vivo)

Niacin（皮下注射，3-300 mg/kg，一次）可在雄性 C57BL/6 小鼠數(shù)分鐘內(nèi)以劑量依賴性方式誘導血管舒張^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6 mice^[3]
Dosage:	3-300 mg/kg
Administration:	Subcutaneous injection; once
Result:	Induced vasodilation in a dose-dependent manner.

Clinical Trial

分子量

123.11

Formula

C₆H₅NO₂

CAS 號

59-67-6

性狀

固體

顏色

White to off-white

中文名稱

維生素B3；煙酸；尼克酸

結(jié)構(gòu)分類

Ketones, Aldehydes, Acids

初始來源

微生物

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Store at room temperature 3 years

In solvent	-80°C	1 year
	-20°C	6 months

溶解性數(shù)據(jù)

細胞實驗：

DMSO 中的溶解度 : 50 mg/mL (406.14 mM; 超聲助溶 (<60°C); 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

H₂O 中的溶解度 : 10 mg/mL (81.23 mM; 超聲助溶)

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	8.1228 mL	40.6138 mL	81.2275 mL
5 mM	1.6246 mL	8.1228 mL	16.2455 mL
10 mM	0.8123 mL	4.0614 mL	8.1228 mL

查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

摩爾計算器
稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動物實驗：

請根據(jù)您的實驗動物和給藥方式選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

方案一

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (16.90 mM); 懸濁液; 超聲助溶

此方案可獲得 2.08 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。
以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (16.90 mM); 澄清溶液

此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液中，混合均勻。
2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
方案三

請依序添加每種溶劑： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (16.90 mM); 澄清溶液

此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。
以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

方案一

請依序添加每種溶劑： PBS
Solubility: 12.5 mg/mL (101.53 mM); 澄清溶液; 超聲助溶

動物溶解方案計算器

請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

每只動物的給藥體積

μL

動物數(shù)量

只

由于實驗過程有損耗，建議您多配一只動物的量

請輸入您的動物體內(nèi)配方組成：

DMSO +

Tween-80 +

Saline

如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。

方案所需 助溶劑 包括：DMSO，，均可在 MCE 網(wǎng)站選購。，Tween 80，均可在 MCE 網(wǎng)站選購。

計算結(jié)果

工作液所需濃度 : mg/mL

儲備液配制方法 : mg 藥物溶于 μL DMSO（母液濃度為 mg/mL）。

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。

動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。

連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。

請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱（超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。

純度 & 產(chǎn)品資料

純度: 99.96%

選擇批次：

Data Sheet (645 KB) SDS (393 KB)

COA (285 KB) HNMR (123 KB) RP-HPLC (225 KB) LCMS (98 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻

[1]. Areeg M Almubarak, et al. Supplementation with Niacin during in vitro maturation improves the quality of porcine embryos. Theriogenology. 2021 Jul 15;169:36-46. doi: 10.1016/j.theriogenology.2021.04.005. Epub 2021 Apr 18. [Content Brief]

[2]. Nobuhiro Hirakawa, et al. Anti-invasive activity of niacin and trigonelline against cancer cells. Biosci Biotechnol Biochem. 2005 Mar;69(3):653-8. [Content Brief]

[3]. Kang Cheng, et al. Antagonism of the prostaglandin D2 receptor 1 suppresses nicotinic acid-induced vasodilation in mice and humans. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6682-7. [Content Brief]

[4]. Bruckert E, et al. Meta-analysis of the effect of nicotinic acid alone or in combination on cardiovascular events and atherosclerosis. Atherosclerosis. 2010 Jun;210(2):353-61. [Content Brief]

[5]. Wan P, et al. Pellagra: a review with emphasis on photosensitivity. Br J Dermatol. 2011 Jun;164(6):1188-200. [Content Brief]

[6]. Ishii N, et al. Pellagra among chronic alcoholics: clinical and pathological study of 20 necropsy cases. J Neurol Neurosurg Psychiatry. 1981 Mar;44(3):209-15. [Content Brief]

Niacin 相關(guān)分類

完整儲備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	8.1228 mL	40.6138 mL	81.2275 mL	203.0688 mL
	5 mM	1.6246 mL	8.1228 mL	16.2455 mL	40.6138 mL
	10 mM	0.8123 mL	4.0614 mL	8.1228 mL	20.3069 mL
	15 mM	0.5415 mL	2.7076 mL	5.4152 mL	13.5379 mL
	20 mM	0.4061 mL	2.0307 mL	4.0614 mL	10.1534 mL
	25 mM	0.3249 mL	1.6246 mL	3.2491 mL	8.1228 mL
	30 mM	0.2708 mL	1.3538 mL	2.7076 mL	6.7690 mL
	40 mM	0.2031 mL	1.0153 mL	2.0307 mL	5.0767 mL
	50 mM	0.1625 mL	0.8123 mL	1.6246 mL	4.0614 mL
	60 mM	0.1354 mL	0.6769 mL	1.3538 mL	3.3845 mL
	80 mM	0.1015 mL	0.5077 mL	1.0153 mL	2.5384 mL
DMSO	100 mM	0.0812 mL	0.4061 mL	0.8123 mL	2.0307 mL

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

Help & FAQs

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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Niacin59-67-6Nicotinic acid Vitamin B3維生素B3煙酸尼克酸Vitamin B3Vitamin B 3Vitamin B-3AutophagyEndogenous Metaboliteapoptosiscardiovascular diseasescancerInhibitorinhibitorinhibit

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Niacin (Synonyms: 煙酸; Nicotinic acid; Vitamin B3)

Customer Review

Other Forms of Niacin:

MCE 顧客使用本產(chǎn)品發(fā)表的 8 篇科研文獻

Niacin 相關(guān)產(chǎn)品

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Niacin (Synonyms: 煙酸; Nicotinic acid; Vitamin B3)

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Niacin 相關(guān)抗體

MCE 顧客使用本產(chǎn)品發(fā)表的 8 篇科研文獻

Niacin 相關(guān)產(chǎn)品

•相關(guān)化合物庫:

•同靶點產(chǎn)品:

•同靶點蛋白產(chǎn)品:

生物活性

純度 & 產(chǎn)品資料

參考文獻

Niacin 相關(guān)抗體:

摩爾計算器

稀釋計算器

Niacin 相關(guān)分類

完整儲備液配制表

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