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  2. 誘導疾病模型產(chǎn)品 Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Anti-infection
  3. 免疫與炎癥疾病模型 Estrogen Receptor/ERR Endogenous Metabolite Bacterial
  4. 半抗原
  5. Estradiol

Estradiol  (Synonyms: 雌二醇; β-Estradiol; E2; 17β-Estradiol; 17β-Oestradiol)

目錄號: HY-B0141 純度: 99.93%
COA 產(chǎn)品使用指南 技術(shù)支持

Estradiol (β-Estradiol) 是一種類固醇激素和主要的女性性激素。Estradiol 可上調(diào)人子宮內(nèi)膜干細胞 (hEnSCs) 神經(jīng)標志物的表達并促進其神經(jīng)分化。Estradiol 可用于癌癥、神經(jīng)退行性疾病和神經(jīng)組織工程的相關(guān)研究。

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Estradiol Chemical Structure

Estradiol Chemical Structure

CAS No. : 50-28-2

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10 mM * 1 mL in DMSO ￥500
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500 mg ￥400
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1 g ￥500
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Other Forms of Estradiol:

MCE 顧客使用本產(chǎn)品發(fā)表的 78 篇科研文獻

查看 Estrogen Receptor/ERR 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Estradiol (β-Estradiol) is a steroid hormone and the major female sex hormone. Estradiol can up-regulate the expression of neural markers of human endometrial stem cells (hEnSCs) and promote their neural differentiation. Estradiol can be used for the research of cancers, neurodegenerative diseases and neural tissue engineering[1][2].

IC50 & Target

Human Endogenous Metabolite

 

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
B16-F10 IC50
39.81 μM
Compound: ES
Antiproliferative activity mouse B16F10 cells after 2 days by MTT assay
Antiproliferative activity mouse B16F10 cells after 2 days by MTT assay
[PMID: 25222876]
CHO IC50
> 100 μM
Compound: ES
Antiproliferative activity against CHO cells after 2 days by MTT assay
Antiproliferative activity against CHO cells after 2 days by MTT assay
[PMID: 25222876]
CHO EC50
38.4 μM
Compound: 55
Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
[PMID: 18307294]
CHO-K1 EC50
0.03 μM
Compound: E2
Agonist activity at ERbeta expressed in CHO-K1 cells by luciferase reporter gene transactivation assay
Agonist activity at ERbeta expressed in CHO-K1 cells by luciferase reporter gene transactivation assay
[PMID: 20553023]
CHO-K1 EC50
0.036 nM
Compound: E2
Transcriptional activation of ERbeta receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay
Transcriptional activation of ERbeta receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay
[PMID: 22647217]
CHO-K1 EC50
0.05 nM
Compound: E2
Transcriptional activation of ERalpha receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay
Transcriptional activation of ERalpha receptor expressed in CHO-K1 cells after 24 hrs by luciferase reporter gene assay
[PMID: 22647217]
CHO-K1 EC50
0.05 μM
Compound: E2
Agonist activity at ERalpha expressed in CHO-K1 cells by luciferase reporter gene transactivation assay
Agonist activity at ERalpha expressed in CHO-K1 cells by luciferase reporter gene transactivation assay
[PMID: 20553023]
COS-7 EC50
0.3 nM
Compound: 17beta-estradiol
Agonist activity at GFP-tagged GPR30 expressed in COS7 cells assessed as half life for increase in intracellular calcium level by spectrofluorimetry
Agonist activity at GFP-tagged GPR30 expressed in COS7 cells assessed as half life for increase in intracellular calcium level by spectrofluorimetry
[PMID: 16520733]
COS-7 EC50
11 nM
Compound: Estradiol
Agonist activity at estrogen receptor beta ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay
Agonist activity at estrogen receptor beta ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay
[PMID: 19863083]
COS-7 EC50
3.2 nM
Compound: Estradiol
Agonist activity at estrogen receptor alpha ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay
Agonist activity at estrogen receptor alpha ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay
[PMID: 19863083]
HEC-1 EC50
0.09 nM
Compound: Estradiol
Agonist activity at human full-length ERalpha receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay
Agonist activity at human full-length ERalpha receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay
[PMID: 21481497]
HEC-1 EC50
0.1 nM
Compound: estradiol
Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay
Agonist activity at ERalpha expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay
[PMID: 19286283]
HEC-1 EC50
0.123 nM
Compound: E2, 17beta-E2, estradiol
Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay
Agonist activity at human ERalpha expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay
[PMID: 22122563]
HEC-1 EC50
0.13 nM
Compound: estradiol
Agonist activity at full-length human estrogen receptor beta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay
Agonist activity at full-length human estrogen receptor beta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay
[PMID: 19128016]
HEC-1 EC50
0.16 nM
Compound: estradiol
Agonist activity at human ERalpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay
Agonist activity at human ERalpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay
[PMID: 25559213]
HEC-1 EC50
0.2 nM
Compound: E2, 17beta-E2, estradiol
Agonist activity at human ERbeta expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay
Agonist activity at human ERbeta expressed in HEC-1 cells coexpressing beta-gal assessed as luciferase activity after 24 hrs by reporter gene assay
[PMID: 22122563]
HEC-1 EC50
0.38 nM
Compound: estradiol
Agonist activity at human ERbeta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay
Agonist activity at human ERbeta expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by ERE-luciferase reporter gene transfection assay
[PMID: 25559213]
HEC-1 EC50
0.5 nM
Compound: estradiol
Agonist activity at ERbeta expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay
Agonist activity at ERbeta expressed in human HEC1 cells assessed as transcriptional potency after 24 hrs by luciferase-beta galactosidase reporter gene assay
[PMID: 19286283]
HEC-1 EC50
0.7 nM
Compound: estradiol
Agonist activity at full-length human estrogen receptor alpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay
Agonist activity at full-length human estrogen receptor alpha expressed in human HEC1 cells assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay
[PMID: 19128016]
HEC-1 EC50
0.72 nM
Compound: Estradiol
Agonist activity at human full-length ERbeta receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay
Agonist activity at human full-length ERbeta receptor expressed in human HEC1 cells co-expressing (ERE)2-pS2-luc gene assessed as transcriptional activation after 24 hrs by luciferase reporter gene assay
[PMID: 21481497]
HEK293 EC50
0.024 nM
Compound: beta-Est
Agonist activity at human ERalpha expressed in HEK293 cells by luciferase reporter gene assay
Agonist activity at human ERalpha expressed in HEK293 cells by luciferase reporter gene assay
[PMID: 25305688]
HEK293 EC50
0.046 nM
Compound: 1
Agonist activity at ERalpha expressed in HEK cells co-expressing beta-lactamase after 18 hrs by beta-lactamase reporter gene assay
Agonist activity at ERalpha expressed in HEK cells co-expressing beta-lactamase after 18 hrs by beta-lactamase reporter gene assay
[PMID: 21885279]
HEK293 EC50
0.085 nM
Compound: beta-Est
Agonist activity at human ERbeta expressed in HEK293 cells by luciferase reporter gene assay
Agonist activity at human ERbeta expressed in HEK293 cells by luciferase reporter gene assay
[PMID: 25305688]
HEK293 EC50
0.09 nM
Compound: E2
Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay
Selective estrogen receptor down-regulator activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ERalpha degradation by luciferase reporter gene assay
[PMID: 30940565]
HEK293 EC50
0.13 nM
Compound: E2
Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay
Agonist activity at FLAG-tagged ERalpha (unknown origin) expressed in HEK293 cells assessed as induction of ER-alpha-mediated transcriptional activity by luciferase reporter gene assay
[PMID: 30940565]
HEK293 EC50
0.75 nM
Compound: estradiol
Agonist activity at ERalpha expressed in HEK293 cells by transactivation assay
Agonist activity at ERalpha expressed in HEK293 cells by transactivation assay
[PMID: 17890084]
HEK293 EC50
0.75 nM
Compound: estradiol
Agonist activity at human recombinant ERalpha expressed in HEK293 cells by transactivation assay
Agonist activity at human recombinant ERalpha expressed in HEK293 cells by transactivation assay
[PMID: 17448656]
HEK293 EC50
2.1 nM
Compound: estradiol
Agonist activity at ERbeta expressed in HEK293 cells by transactivation assay
Agonist activity at ERbeta expressed in HEK293 cells by transactivation assay
[PMID: 17890084]
HEK293 EC50
2.1 nM
Compound: estradiol
Agonist activity at human recombinant ERbeta expressed in HEK293 cells by transactivation assay
Agonist activity at human recombinant ERbeta expressed in HEK293 cells by transactivation assay
[PMID: 17448656]
HEK293 EC50
4.6 nM
Compound: E2
Agonist activity at ZFP-fused ERRbeta LBD expressed in HEK293 cells by TR-FRET assay
Agonist activity at ZFP-fused ERRbeta LBD expressed in HEK293 cells by TR-FRET assay
[PMID: 21218783]
HEK293 IC50
50 μM
Compound: Estradiol
Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit
Inhibition of L-type calcium channel measured using 2-electrode voltage-clamp in human embryonic kidney cells heterologically expressing alpha-1C subunit
[PMID: 22761000]
HEK-293T EC50
10.4 nM
Compound: E2
Agonist activity at human ERbeta E305A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay
Agonist activity at human ERbeta E305A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay
[PMID: 20659801]
HEK-293T EC50
2.5 nM
Compound: E2
Agonist activity at human ERalpha E353A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay
Agonist activity at human ERalpha E353A mutant expressed in HEK293T cells co-expressing ERE assessed as transactivation activity by luciferase reporter gene assay
[PMID: 20659801]
HeLa EC50
0.21 nM
Compound: E2
Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay
Agonist activity at ERalpha (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay
[PMID: 31879182]
HeLa EC50
0.37 nM
Compound: E2
Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay
Agonist activity at ERbeta (unknown origin) expressed in human HeLa cells assessed as transcriptional activation measured after 24 hrs by luciferase reporter gene assay
[PMID: 31879182]
HeLa EC50
0.7 nM
Compound: 38
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 37077378]
Hepatocyte EC50
1.9 μM
Compound: Estradiol
Induction of CYP2B6 mRNA expression in human donor hepatocytes after 48 hrs by real-time PCR method
Induction of CYP2B6 mRNA expression in human donor hepatocytes after 48 hrs by real-time PCR method
[PMID: 22815312]
Hepatocyte EC50
4.1 μM
Compound: Estradiol
Induction of CYP2B6 activity in human donor hepatocytes assessed as hydroxybupropion formation after 48 hrs by LC/MS method
Induction of CYP2B6 activity in human donor hepatocytes assessed as hydroxybupropion formation after 48 hrs by LC/MS method
[PMID: 22815312]
HepG2 EC50
11 nM
Compound: E2, estradiol
Agonist activity at human ERbeta expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay
Agonist activity at human ERbeta expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay
[PMID: 22283328]
HepG2 EC50
2.2 nM
Compound: E2, estradiol
Agonist activity at human ERalpha expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay
Agonist activity at human ERalpha expressed in HepG2 cells after 24 hrs by luciferase reporter gene assay
[PMID: 22283328]
Ishikawa EC50
0.047 nM
Compound: 17-beta-estradiol
Estrogenic activity at estrogen receptor in human Ishikawa Var-1 cells assessed as stimulation of alkaline phosphatase activity measured by metabolism of p-nitrophenol phosphatase after 72 hrs
Estrogenic activity at estrogen receptor in human Ishikawa Var-1 cells assessed as stimulation of alkaline phosphatase activity measured by metabolism of p-nitrophenol phosphatase after 72 hrs
[PMID: 15568770]
Ishikawa EC50
0.9 nM
Compound: E2
Agonist activity at estrogen receptor in human Ishikawa cells assessed as increase of alkaline phosphatase level after 3 days using P-nitrophenyl phosphate as substrate
Agonist activity at estrogen receptor in human Ishikawa cells assessed as increase of alkaline phosphatase level after 3 days using P-nitrophenyl phosphate as substrate
[PMID: 22178552]
Ishikawa EC50
24.6 pM
Compound: E2
Estrogenic activity at estrogen receptor in human Ishikawa cells assessed as induction of alkaline phosphatase expression after 72 hrs by microplate reader
Estrogenic activity at estrogen receptor in human Ishikawa cells assessed as induction of alkaline phosphatase expression after 72 hrs by microplate reader
[PMID: 22464681]
JEG-3 IC50
84000 nM
Compound: 3
Estrone sulfatase activity against homogenized human JEG-3 cells was determined by measuring the [3H]E1 obtained from [3H]E1S
Estrone sulfatase activity against homogenized human JEG-3 cells was determined by measuring the [3H]E1 obtained from [3H]E1S
[PMID: 9873454]
MCF7 EC50
0.0084 nM
Compound: estradiol
Agonist activity at human ERalpha in human MCF7 cells assessed as progesterone receptor endogenous genes activation after 24 hrs by qPCR
Agonist activity at human ERalpha in human MCF7 cells assessed as progesterone receptor endogenous genes activation after 24 hrs by qPCR
[PMID: 25559213]
MCF7 EC50
0.01 nM
Compound: 1 (17-beta-estradiol)
Effective concentration against MCF-7 breast tumor cells using MCF-7 assay.
Effective concentration against MCF-7 breast tumor cells using MCF-7 assay.
[PMID: 12825935]
MCF7 EC50
0.3 nM
Compound: estradiol
Agonist activity at human ERbeta in human MCF7 cells assessed as otubain 2 endogenous genes activation after 24 hrs by qPCR
Agonist activity at human ERbeta in human MCF7 cells assessed as otubain 2 endogenous genes activation after 24 hrs by qPCR
[PMID: 25559213]
MCF7 IC50
0.4 nM
Compound: estrogen
Inhibition of chemoattractant-induced cell migration of human MCF7 cells after 48 hrs by transwell migration assay
Inhibition of chemoattractant-induced cell migration of human MCF7 cells after 48 hrs by transwell migration assay
[PMID: 16520733]
MCF7 EC50
10 nM
Compound: E2
Agonist activity at human ERalpha LBD in human MCF7 cells assessed as induction of cell proliferation after up to 6 days by celltiter-glo assay
Agonist activity at human ERalpha LBD in human MCF7 cells assessed as induction of cell proliferation after up to 6 days by celltiter-glo assay
[PMID: 21218783]
MCF7 IC50
27.35 μM
Compound: ES
Antiproliferative activity human ER-positive MCF7 cells after 2 days by MTT assay
Antiproliferative activity human ER-positive MCF7 cells after 2 days by MTT assay
[PMID: 25222876]
MCF7 EC50
3.7 x 10-12 M
Compound: estradiol
Estrogenic activity in human MCF7 cells assessed as cell proliferation after 6 days by WST8 assay
Estrogenic activity in human MCF7 cells assessed as cell proliferation after 6 days by WST8 assay
[PMID: 18760603]
MCF7 IC50
32 μM
Compound: estradiol
Inhibitory concentration against MCF-7 (ER+) cell line using SRB assay
Inhibitory concentration against MCF-7 (ER+) cell line using SRB assay
[PMID: 12565971]
MCF7 EC50
6.4 pM
Compound: E2
Antiproliferative activity against estrogen-deficient human MCF7 cells after 72 hrs by MTT assay
Antiproliferative activity against estrogen-deficient human MCF7 cells after 72 hrs by MTT assay
[PMID: 22464681]
MCF7 EC50
8.0 x 10-11 M
Compound: estradiol
Effective concentration against human ER positive MCF-7 breast cancer cell line evaluated by luciferase assay
Effective concentration against human ER positive MCF-7 breast cancer cell line evaluated by luciferase assay
[PMID: 16190777]
MDA-MB-231 IC50
> 100 μM
Compound: estradiol
Inhibitory concentration against MDA-MB-231 (ER-) cell line using SRB assay
Inhibitory concentration against MDA-MB-231 (ER-) cell line using SRB assay
[PMID: 12565971]
MDA-MB-231 IC50
33.46 μM
Compound: ES
Antiproliferative activity human ER-negative MDA-MB-231 cells after 2 days by MTT assay
Antiproliferative activity human ER-negative MDA-MB-231 cells after 2 days by MTT assay
[PMID: 25222876]
MIA PaCa-2 IC50
42.93 μM
Compound: ES
Antiproliferative activity human MIAPaCa2 cells after 2 days by MTT assay
Antiproliferative activity human MIAPaCa2 cells after 2 days by MTT assay
[PMID: 25222876]
NIH3T3 IC50
> 100 μM
Compound: ES
Antiproliferative activity mouse NIH/3T3 cells after 2 days by MTT assay
Antiproliferative activity mouse NIH/3T3 cells after 2 days by MTT assay
[PMID: 25222876]
SAOS-2 ED50
4.7 nM
Compound: 2
Functional Estrogen receptor beta activity by metallothionein induction in SAOS-2 cells
Functional Estrogen receptor beta activity by metallothionein induction in SAOS-2 cells
[PMID: 12824043]
SK-BR-3 IC50
0.1 nM
Compound: estrogen
Inhibition of chemoattractant-induced cell migration of human SK-BR-3 cells after 48 hrs by transwell migration assay
Inhibition of chemoattractant-induced cell migration of human SK-BR-3 cells after 48 hrs by transwell migration assay
[PMID: 16520733]
U2OS EC50
0.004 nM
Compound: E2
Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control
Activation of human ERalpha expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control
[PMID: 23043242]
U2OS EC50
0.01 nM
Compound: E2
Activation of human ERbeta expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control
Activation of human ERbeta expressed in human U2-OS cells by luciferase reporter gene assay relative to untreated control
[PMID: 23043242]
U2OS EC50
0.019 nM
Compound: estradiol
Transcriptional activation of estrogen receptor alpha in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT
Transcriptional activation of estrogen receptor alpha in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT
[PMID: 16162002]
U2OS EC50
0.098 nM
Compound: estradiol
Transcriptional activation of estrogen receptor beta in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT
Transcriptional activation of estrogen receptor beta in U2OS cell using estrogen-regulated luciferase reporter gene plasmid upon incubation for 20-30 mins at RT
[PMID: 16162002]
U2OS EC50
0.15 nM
Compound: E2, 17beta-E2, estradiol
Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay
Agonist activity at human ERalpha expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay
[PMID: 22122563]
U2OS EC50
0.4 nM
Compound: E2, 17beta-E2, estradiol
Agonist activity at human ERbeta expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay
Agonist activity at human ERbeta expressed in U2OS cells coexpressing pCMX-hSRC3 assessed as luciferase activity after 24 hrs by reporter gene assay
[PMID: 22122563]
體外研究
(In Vitro)

Estradiol (10 nM，7 d) 誘導人子宮內(nèi)膜干細胞 (hEnSCs) 的神經(jīng)分化和增加軸突分支[1]。
Estradiol (17β-estradiol，10 nM，7 days) 增加 hEnSCs 分化的神經(jīng)樣細胞中神經(jīng)元樣細胞標志物 (Tuj-1、巢蛋白和 NF-H) 的表達[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Differentiation Assay[1]

Cell Line: Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
Concentration: 10 nM
Incubation Time: 7 days
Result: Increased the number of neurite processes including neural differentiation and neurite branching.

Immunofluorescence[1]

Cell Line: Isolated human endometrial stem cells (hEnSCs) from human endometrial tissue
Concentration: 10 nM
Incubation Time: 7 days
Result: Increased the percentage of neural marker (Tuj-1, nestin and NF-H)-positive cells of 62.2±1.3%, 71.5±4% and 51.2±1.5% respectively.
體內(nèi)研究
(In Vivo)

Estradiol (1 nM，來自 FBN-ARO-KO 小鼠的海馬切片) 挽救長期增強 (LTP) 振幅[1]。
Estradiol (0.0167 mg，皮下植入，F(xiàn)BN-ARO-KO 小鼠) 挽救 FBN-ARO-KO 小鼠的分子和功能缺陷[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: FBN-ARO-KO Mice[2]
Dosage: 1 nM
Administration: Treated for the hippocampal slices
Result: Rescued long-term potentiation (LTP) amplitude of both male and female mice.
Animal Model: FBN-ARO-KO Mice[2]
Dosage: 0.0167 mg
Administration: Alzet minipumps with Estradiol (implanted s.c.), examined 7 days after minipump implantation.
Result: Restored hippocampal and cortical E2 levels to 93%, phosphorylation of AKT, ERK and CREB in the hippocampus and cortex to 90-95%, BDNF level to 80-90%, restored both synaptophysin and PSD95 in the forebrain.
Rescued the spatial learning and memory defects.
Clinical Trial
分子量

272.38

Formula

C18H24O2
CAS 號
性狀

固體

顏色

White to off-white

中文名稱

雌二醇；強力求偶素
結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 1 year
-20°C 6 months
溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : 100 mg/mL (367.13 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

Ethanol 中的溶解度 : 20 mg/mL (73.43 mM; 超聲助溶)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.6713 mL 18.3567 mL 36.7134 mL
5 mM 0.7343 mL 3.6713 mL 7.3427 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.18 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 5% DMSO    40% PEG300    5% Tween-80    50% Saline

    Solubility: 2.5 mg/mL (9.18 mM); 懸濁液; 超聲助溶

以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

  • 方案 一

    請依序添加每種溶劑： Corn Oil

    Solubility: 12.5 mg/mL (45.89 mM); 懸濁液; 超聲助溶

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.93%

參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 3.6713 mL 18.3567 mL 36.7134 mL 91.7835 mL
5 mM 0.7343 mL 3.6713 mL 7.3427 mL 18.3567 mL
10 mM 0.3671 mL 1.8357 mL 3.6713 mL 9.1784 mL
15 mM 0.2448 mL 1.2238 mL 2.4476 mL 6.1189 mL
20 mM 0.1836 mL 0.9178 mL 1.8357 mL 4.5892 mL
25 mM 0.1469 mL 0.7343 mL 1.4685 mL 3.6713 mL
30 mM 0.1224 mL 0.6119 mL 1.2238 mL 3.0595 mL
40 mM 0.0918 mL 0.4589 mL 0.9178 mL 2.2946 mL
50 mM 0.0734 mL 0.3671 mL 0.7343 mL 1.8357 mL
60 mM 0.0612 mL 0.3059 mL 0.6119 mL 1.5297 mL
DMSO 80 mM 0.0459 mL 0.2295 mL 0.4589 mL 1.1473 mL
100 mM 0.0367 mL 0.1836 mL 0.3671 mL 0.9178 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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