成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

  2. Cell Cycle/DNA Damage Autophagy Apoptosis
  3. DNA/RNA Synthesis Nucleoside Antimetabolite/Analog Autophagy Apoptosis
  4. Gemcitabine hydrochloride

Gemcitabine hydrochloride  (Synonyms: 鹽酸吉西他濱; LY 188011 hydrochloride)

目錄號: HY-B0003 純度: 99.89%
COA 產(chǎn)品使用指南 技術(shù)支持

Gemcitabine Hydrochloride (LY 188011 Hydrochloride) 是一種嘧啶核苷類似物抗代謝藥 (nucleoside antimetabolite/analog) 和抗腫瘤劑。Gemcitabine 抑制 DNA 合成 (DNA synthesis) 和修復(fù)，導(dǎo)致細(xì)胞自噬 (autophagy) 和凋亡 (apoptosis)。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Gemcitabine hydrochloride Chemical Structure

Gemcitabine hydrochloride Chemical Structure

CAS No. : 122111-03-9

1.  客戶無需承擔(dān)相應(yīng)的運輸費用。

2.  同一機構(gòu)（單位）同一產(chǎn)品試用裝僅限申領(lǐng)一次，同一機構(gòu)（單位）一年內(nèi)

     可免費申領(lǐng)三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥792
In-stock
100 mg ￥720
In-stock
200 mg ￥1080
In-stock
500 mg ￥1980
In-stock
1 g ￥3100
In-stock
5 g   詢價  
10 g   詢價  

* Please select Quantity before adding items.

Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 172 篇科研文獻

WB
Proliferation Assay

    Gemcitabine hydrochloride purchased from MCE. Usage Cited in: Naunyn Schmiedebergs Arch Pharmacol. 2019 May;392(5):615-622.  [Abstract]

    The expression level of p-JAK2, p-STAT3, Bcl-XL, and Mcl-1 in PANC-1 cells is detected by western blot analysis and is all down-regulated in the treatment groups.

    Gemcitabine hydrochloride purchased from MCE. Usage Cited in: Chem Biol Interact. 2018 Jun 25;290:44-51.  [Abstract]

    U2OS and MG-63 cells are treated with Gemcitabine, Licoricidin, or Gemcitabine+Licoricidin for 24 h, followed by the determination of active caspse-3 protein level using western blot. Cells without treatment are used as Control.

    Gemcitabine hydrochloride purchased from MCE. Usage Cited in: J Biol Chem. 2017 Jun 2;292(22):9136-9149.  [Abstract]

    The plate clone formation of SW480 and SW620 cells with Gemcitabine (8 nM in SW480 and 16 nM in SW620) and/or PX-12 (4 μM in SW480 and 8 μM in SW620).

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Gemcitabine Hydrochloride (LY 188011 Hydrochloride) is a pyrimidine nucleoside analog antimetabolite and an antineoplastic agent. Gemcitabine Hydrochloride inhibits DNA synthesis and repair, resulting in autophagyand apoptosis[1][2].

    IC50 & Target

    DNA synthesis[1]
    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    A2780 IC50
    1.25 μM
    Compound: GEM
    Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 33992863]
    A2780 IC50
    1.38 μM
    Compound: GEM
    Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    		[PMID: 33992863]
    A549 IC50
    14.65 μM
    Compound: GEM
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    		[PMID: 33992863]
    A549 IC50
    2.31 μM
    Compound: GEM
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 33992863]
    C2D cell line IC50
    11.9 nM
    Compound: Gemzar
    Inhibitory concentration to inhibit growth of C2D prostate cancer cell line by using MTT assay
    Inhibitory concentration to inhibit growth of C2D prostate cancer cell line by using MTT assay
    		[PMID: 14971910]
    C2G cell line IC50
    3.7 nM
    Compound: Gemzar
    Inhibitory concentration to inhibit growth of C2G prostate cancer cell line by using MTT assay
    Inhibitory concentration to inhibit growth of C2G prostate cancer cell line by using MTT assay
    		[PMID: 14971910]
    L02 IC50
    11.87 μM
    Compound: GEM
    Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 33992863]
    L02 IC50
    4.51 μM
    Compound: GEM
    Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs in hypoxic condition by MTT assay
    		[PMID: 33992863]
    MCF7 IC50
    6.35 μM
    Compound: GEM
    Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
    		[PMID: 33992863]
    NCI-H460 IC50
    2.13 nM
    Compound: Gemcitabine HCl
    Cytotoxicity against human NCI-H460 cells by MTT assay
    Cytotoxicity against human NCI-H460 cells by MTT assay
    		[PMID: 19345581]
    NCI-H460 IC50
    2.13 μM
    Compound: GemcitabineHCl
    Cytotoxicity against human NCI-H460 cells after 3 days by MTT assay
    Cytotoxicity against human NCI-H460 cells after 3 days by MTT assay
    		[PMID: 20356655]
    TRAMP-C1A IC50
    9 nM
    Compound: Gemzar
    Inhibitory concentration to inhibit growth of C1A prostate cancer cell line by using MTT assay
    Inhibitory concentration to inhibit growth of C1A prostate cancer cell line by using MTT assay
    		[PMID: 14971910]
    TRAMP-C2H IC50
    4.7 nM
    Compound: Gemzar
    Inhibitory concentration to inhibit growth of C2H prostate cancer cell line by using MTT assay
    Inhibitory concentration to inhibit growth of C2H prostate cancer cell line by using MTT assay
    		[PMID: 14971910]
    Vero C1008 CC50
    23.22 μM
    Compound: Gemcitabine Hydrochloride
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.004 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    Vero C1008 CC50
    23.22 μM
    Compound: Gemcitabine Hydrochloride
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    CC50 determination at MOI 0.01 using CellTiter- Glo (CTG) assay, performed 3 days post-infection in Vero E6 cells
    		10.1101/2020.03.25.008482
    體外研究
    (In Vitro)

    Gemcitabine Hydrochloride (購自 MedChem Express，0.003-1 μM；3 天) 可有效、強效地殺死小鼠和人類衰老細(xì)胞[4]。
    Gemcitabine Hydrochloride 抑制 BxPC-3、Mia Paca-2、PANC-1、PL-45 和 AsPC-1 細(xì)胞的生長，IC50分別為 37.6、42.9、92.7、89.3 和 131.4 nM[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[4]

    Cell Line: Non-senescent and replication-induced senescent new born dermal fibroblasts (NBFs)
    Concentration: 0.003, 0.01, 0.03, 0.1, 0.3, 1 μM
    Incubation Time: 3 days
    Result: Killed replication-induced senescent NBFs for 3 days with 11.0% cell viability.
    體內(nèi)研究
    (In Vivo)

    Gemcitabine Hydrochloride 可通過氣管內(nèi)噴霧劑給藥于大鼠，且無明顯毒性，最大耐受劑量為每周一次 4 mg/kg，連續(xù) 9 周。Gemcitabine Hydrochloride 通過肺部給藥的毒性低于口服給藥，劑量為 2、4 和 6 mg/kg[2]。
    使用 Gemcitabine Hydrochloride (50 mg/kg，i.p.) 或 DMAPT/Gemcitabine Hydrochloride l 聯(lián)合治療 LSL-KrasG12D/+、LSL-Trp53R172H 和 Pdx-1-Cre 小鼠可顯著延長中位生存期 30 多天，而安慰劑組則分別為 254.5 天、255 天和 217.5 天[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    299.66

    Formula

    C9H12ClF2N3O4
    CAS 號
    性狀

    固體

    顏色

    White to off-white

    中文名稱

    鹽酸吉西他濱；吉西他賓鹽酸鹽
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)

    溶解性數(shù)據(jù)
    細(xì)胞實驗： 

    DMSO 中的溶解度 : 62.5 mg/mL (208.57 mM; 超聲助溶 (<60°C); 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    H2O 中的溶解度 : 25 mg/mL (83.43 mM; 超聲助溶)

    DMF 中的溶解度 : 2.5 mg/mL (8.34 mM; 超聲助溶)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 3.3371 mL 16.6856 mL 33.3712 mL
    5 mM 0.6674 mL 3.3371 mL 6.6742 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (6.94 mM); 澄清溶液

      此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (6.94 mM); 澄清溶液

      此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

    以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請依序添加每種溶劑： PBS

      Solubility: 25 mg/mL (83.43 mM); 澄清溶液; 超聲助溶

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    計算結(jié)果
    工作液所需濃度 : mg/mL
    該產(chǎn)品水溶性佳，請具體參考實測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    純度 & 產(chǎn)品資料

    純度: 99.93%

    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture and light)。-80°C儲存時，請在1年內(nèi)使用， -20°C儲存時，請在6個月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMF / H2O / DMSO 1 mM 3.3371 mL 16.6856 mL 33.3712 mL 83.4279 mL
    5 mM 0.6674 mL 3.3371 mL 6.6742 mL 16.6856 mL
    H2O / DMSO 10 mM 0.3337 mL 1.6686 mL 3.3371 mL 8.3428 mL
    15 mM 0.2225 mL 1.1124 mL 2.2247 mL 5.5619 mL
    20 mM 0.1669 mL 0.8343 mL 1.6686 mL 4.1714 mL
    25 mM 0.1335 mL 0.6674 mL 1.3348 mL 3.3371 mL
    30 mM 0.1112 mL 0.5562 mL 1.1124 mL 2.7809 mL
    40 mM 0.0834 mL 0.4171 mL 0.8343 mL 2.0857 mL
    50 mM 0.0667 mL 0.3337 mL 0.6674 mL 1.6686 mL
    60 mM 0.0556 mL 0.2781 mL 0.5562 mL 1.3905 mL
    80 mM 0.0417 mL 0.2086 mL 0.4171 mL 1.0428 mL
    DMSO 100 mM 0.0334 mL 0.1669 mL 0.3337 mL 0.8343 mL

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    您最近查看的產(chǎn)品:

    Your information is safe with us. * Required Fields.

       產(chǎn)品名稱：

    		  

    * 需求量：

    * 客戶姓名：

    		 

    * Email：

    * 電話：

    		 

    * 公司或機構(gòu)名稱：

       留言給我們：

    Bulk Inquiry

    Inquiry Information

    產(chǎn)品名稱：
    Gemcitabine hydrochloride
    目錄號：
    HY-B0003
    需求量：