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  2. PI3K/Akt/mTOR Autophagy Metabolic Enzyme/Protease
  3. PI3K Autophagy Mitophagy Endogenous Metabolite
  4. 3-Methyladenine

3-Methyladenine  (Synonyms: 3-甲基腺嘌呤; 3-MA)

目錄號(hào): HY-19312 純度: 99.91%
COA 產(chǎn)品使用指南 技術(shù)支持

3-Methyladenine (3-MA) 是 PI3K 的抑制劑。它通過(guò)抑制class III PI3K廣泛作為自噬 (autophagy) 的抑制劑使用。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

3-Methyladenine Chemical Structure

3-Methyladenine Chemical Structure

CAS No. : 5142-23-4

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規(guī)格 價(jià)格 是否有貨 數(shù)量
50 mg ￥550
In-stock
100 mg ￥800
In-stock
200 mg ￥1400
In-stock
500 mg ￥2800
In-stock
1 g   詢價(jià)  
5 g   詢價(jià)  

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Customer Review

Other Forms of 3-Methyladenine:

MCE 顧客使用本產(chǎn)品發(fā)表的 811 篇科研文獻(xiàn)

WB
IF

    3-Methyladenine purchased from MCE. Usage Cited in: J Nanobiotechnology. 2023 Feb 28;21(1):69.  [Abstract]

    The autophagy is inhibited by 3-Methyladenine (3-MA; 5 mM; 24 h), as evidenced by decreased Atg5 expression and the ratio of LC3-II/LC3-I and increased p62 protein level in MLE-12 cells (Fig. B, C).

    3-Methyladenine purchased from MCE. Usage Cited in: J Nanobiotechnology. 2023 Feb 28;21(1):69.  [Abstract]

    3-Methyladenine (3-MA; 5 mM; 24 h) markedly decreases the SWCNTs-induced increase in LC3B puncta, in MLE-12 cells.

    3-Methyladenine purchased from MCE. Usage Cited in: Brain Behav Immun. 2019 Jul;79:244-255.  [Abstract]

    Immunoblot analysis of NLRP3 in LPS-primed macrophages treated with KA (10 μM) or concurrently with autophagy inhibitor (3-MA, 5 mM). Statistical analysis of NLRP3 intensity during 3-MA or zaprinast treatment is shown.

    3-Methyladenine purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119.  [Abstract]

    Western blotting image protein levels of GRP78/Bip in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

    3-Methyladenine purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119.  [Abstract]

    Western blotting image protein levels of LC3-I & LC3-II in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

    3-Methyladenine purchased from MCE. Usage Cited in: Ecotoxicol Environ Saf. 2019 Apr 15;170:112-119.  [Abstract]

    Western blotting image protein levels of p62 in DF-1 cells treated with different supplements (+ represent supplying, - represents not supplying a certain amount of Cr(VI), 4-PBA, Tg, NS-398, 3-MA and Rapamycin).

    3-Methyladenine purchased from MCE. Usage Cited in: Front Endocrinol (Lausanne). 2019 Jun 17;10:370.  [Abstract]

    LC3 II expression is regulated in CQ- or 3-MA-treated GnRH-OE Panc1 cells.

    3-Methyladenine purchased from MCE. Usage Cited in: FASEB J. 2019 Apr;33(4):5520-5534.  [Abstract]

    CRC cells are treated with vehicle, BFA (100 nM), 3-MA (10 mM), or in combination for 24 h. Immunoblot analysis is used to detect LC3 turnover.

    3-Methyladenine purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Mar;120(3):4366-4374.  [Abstract]

    Representative Western blots show LC3 I/II and p62 protein expression in kidneys of vehicle group and 3-MA group mice at 72 hours after the CDDP treatment.

    3-Methyladenine purchased from MCE. Usage Cited in: Int J Mol Med. 2018 Jun;41(6):3221-3230.  [Abstract]

    Western blot analysis is used to measure LC3, p62 and Beclin?1 expression following the treatment of H9C2 cells with si?miRNA?30e and 3?MA.

    3-Methyladenine purchased from MCE. Usage Cited in: Int J Mol Med. 2018 May;41(5):2535-2544.  [Abstract]

    MC3T3-E1 cells are treated with AICAR (10 μM) in the presence or absence of 3-MA (5 mM) or CQ (10 μM) for 24 h, after which western blot analysis is conducted.

    3-Methyladenine purchased from MCE. Usage Cited in: J Cell Mol Med. 2018 Oct;22(10):5132-5144.  [Abstract]

    Melatonin regulation of mitophagy activity in the diabetic myocardium, Protein expression with representative gel blots of LC3 I/II, GAPDH (loading control).

    3-Methyladenine purchased from MCE. Usage Cited in: Int J Oncol. 2018 Sep;53(3):1363-1373.  [Abstract]

    The protein expression levels of LC3 and Beclin-1 proteins in HCT-116 cells. Cells are treated with 100 μg/mL active fraction of clove (AFC) for 48 h with or without pretreatment of 2 mM 3-MA or 1 nM Baflomycin A1 (BA) for 1 h.

    3-Methyladenine purchased from MCE. Usage Cited in: J Dermatol Sci. 2018 Jan;89(1):11-18.  [Abstract]

    Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA. Autophagy is decreased in 3-MA-treated group, which is reflected by the decrease in the ratio of LC3 II/LC3 I and increase in p62 protein

    3-Methyladenine purchased from MCE. Usage Cited in: J Dermatol Sci. 2018 Jan;89(1):11-18.  [Abstract]

    Inhibitory effects of LED 585 nm on melanogenesis is reversed by 3-MA.

    3-Methyladenine purchased from MCE. Usage Cited in: J Immunol Res. 2018 Dec 24;2018:9807139.  [Abstract]

    Western blot assays show that 3-MA abrogates the Alda-1 induced expression increases in LC3BⅡ and Bcl2 and decreases in P62 and Bax.

    3-Methyladenine purchased from MCE. Usage Cited in: Acta Biochim Biophys Sin (Shanghai). 2018 Feb 1;50(2):144-155.  [Abstract]

    Autophagy is involved in Rg1-inhibited apoptosis in macrophages Macrophages with serum deprivation are treated with 50 μM Rg1 for 48 h in the absence or presence of 3-MA (5 mM). The protein expression level of cleaved caspase-3 determined by western blot analysis in Raw264.7 macrophages.

    3-Methyladenine purchased from MCE. Usage Cited in: PLoS One. 2018 Aug 6;13(8):e0201920.  [Abstract]

    The pretreatment of Z-VAD-FMK significantly reduces the levels of cleaved caspase-3 and cleaved PARP. The pretreatment of 3-MA blocks the conversion of LC3-I to LC3-II.

    3-Methyladenine purchased from MCE. Usage Cited in: Biomed Res. 2018;39(2):87-94.  [Abstract]

    Accumulation of LC3B II and p62 proteins in the livers of chloroform-treated mice is reduced by 3-MA treatment.

    3-Methyladenine purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2018;48(6):2318-2336.  [Abstract]

    Cells are pretreated with 5 mM 3-MA or 2 μM for 1 h and then exposed to 40 μM EPA and 10 μM Rp for 48 h. Cell extracts are prepared and subjected to western blot analysis.

    3-Methyladenine purchased from MCE. Usage Cited in: Oncotarget. 2017 Nov 22;8(65):109135-109150.  [Abstract]

    Cell morphology of MCF-7 treated with RA (20 μM)+ω-3 PUFAs (80 μM) with or without 3-MA (5 mM) for 24h.

    3-Methyladenine purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96.  [Abstract]

    The hepatic stellate cells (HSCs) activation is down-regulated along with autophagy following DMKG or 3MA treatment in vitro. Western blot analysis of LC3B, Beclin-1, α-SMA, and collagen-I expression from lysates of HSC-T6 incubated with 4mM DMKG or 10 mM 3MA for 12 h.

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K[1].

    IC50 & Target[1]

    PtdIns3Kγ

    60 μM (IC50, Cell Assay)

    Vps34

    25 μM (IC50, Cell Assay)

    Autophagy

     

    Mitophagy

     

    Human Endogenous Metabolite

     

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    MIA PaCa-2 EC50
    > 100 μM
    Compound: 3 mA
    Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay
    Antiproliferative activity against human MIA PaCa-2 cells assessed as cell viability measured after 72 hrs by MTT assay
    		[PMID: 28105281]
    體外研究
    (In Vitro)

    3-Methyladenine (0-10 mM；0-48 小時(shí)) 以時(shí)間和劑量依賴性方式誘導(dǎo) HeLa 細(xì)胞中半胱天冬酶依賴性細(xì)胞死亡[2]。
    3-Methyladenine (5 mM；24 小時(shí)) 抑制 HeLa 細(xì)胞在無(wú)葡萄糖條件和正常條件下的自噬[2]。
    3-Methyladenine (5 mM；0-48 小時(shí)) 抑制轉(zhuǎn)化 LC3-I 與 LC3-II (自噬標(biāo)記物) 在 12 小時(shí)和 48 小時(shí)之間的變化，證實(shí)了對(duì)自噬的抑制作用[2]。
    3-Methyladenine 誘導(dǎo)細(xì)胞死亡與自噬抑制無(wú)關(guān)[2]。
    3-Methyladenine 顯著縮短了諾考達(dá)唑誘導(dǎo)的前中期停滯的持續(xù)時(shí)間[2]。
    注意：
    在細(xì)胞培養(yǎng)中，3-MA 的推薦濃度約為 0.5-10 mM。不推薦使用 DMSO 儲(chǔ)備液。我們建議您在實(shí)驗(yàn)前稱量您需要的 3-MA 的量，然后將其溶解在培養(yǎng)基中，并用 0.22 μm 過(guò)濾器除菌，現(xiàn)用現(xiàn)配。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[2]

    Cell Line: HeLa cells
    Concentration: 0 mM, 2.5 mM or 5 mM, 10 mM
    Incubation Time: 0 hour, 24 hours and 48 hours
    Result: Decreased cell viability in a time-and dose-dependent manner, and was associated with caspase-3 activation.

    Cell Autophagy Assay[2]

    Cell Line: HeLa cells
    Concentration: 5 mM
    Incubation Time: 24 hours
    Result: Suppressed autophagy in HeLa cells under both glucose-free conditions and normal conditions.

    Western Blot Analysis[2]

    Cell Line: HeLa cells
    Concentration: 5 mM
    Incubation Time: 0 hour, 12 hours, 24 hours and 48 hours
    Result: Suppressed conversion of LC3-I to LC3-II between 12 hours and 48 hours.
    體內(nèi)研究
    (In Vivo)

    3-Methyladenine (1.5 mg/100 g；腹膜內(nèi)注射；3-24 小時(shí)) 處理可在 12 小時(shí)和 24 小時(shí)時(shí)減輕大鼠牛磺膽酸鈉誘導(dǎo)的嚴(yán)重急性胰腺炎 (SAP)[3]。
    3-Methyladenine 抑制?；悄懰徕c誘導(dǎo)的SAP胰腺腺泡細(xì)胞自噬[3]。
    3-Methyladenine 對(duì)PI3K/Akt信號(hào)通路和NF-?；悄懰徕c誘導(dǎo) SAP 中的 κB 信號(hào)通路[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: 10–12 weeks Specific pathogen free- (SPF-) grade healthy male Sprague-Dawley (SD) rats (250–290?g)[3]
    Dosage: 1.5?mg/100?g (1000 μM)
    Administration: Intraperitoneal injection
    Result: Alleviated Sodium Taurocholate-Induced SAP.
    分子量

    149.15

    Formula

    C6H7N5
    CAS 號(hào)
    性狀

    固體

    顏色

    White to off-white

    中文名稱

    3-甲基腺嘌呤
    結(jié)構(gòu)分類
    初始來(lái)源
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    H2O 中的溶解度 : 5 mg/mL (33.52 mM; 超聲助溶)

    DMSO 中的溶解度 : 8.33 mg/mL (55.85 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 6.7047 mL 33.5233 mL 67.0466 mL
    5 mM 1.3409 mL 6.7047 mL 13.4093 mL
    查看完整工作液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶。

    • 方案 一

      請(qǐng)依序添加每種溶劑： PBS

      Solubility: 4 mg/mL (26.82 mM); 澄清溶液; 超聲助溶

    • 方案 二

      請(qǐng)依序添加每種溶劑： 50% PEG300    50% Saline

      Solubility: 25 mg/mL (167.62 mM); 澄清溶液; 超聲助溶 (<50°C)

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    純度 & 產(chǎn)品資料

    純度: 99.91%

    參考文獻(xiàn)

    完整工作液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 6.7047 mL 33.5233 mL 67.0466 mL 167.6165 mL
    5 mM 1.3409 mL 6.7047 mL 13.4093 mL 33.5233 mL
    10 mM 0.6705 mL 3.3523 mL 6.7047 mL 16.7617 mL
    15 mM 0.4470 mL 2.2349 mL 4.4698 mL 11.1744 mL
    20 mM 0.3352 mL 1.6762 mL 3.3523 mL 8.3808 mL
    25 mM 0.2682 mL 1.3409 mL 2.6819 mL 6.7047 mL
    30 mM 0.2235 mL 1.1174 mL 2.2349 mL 5.5872 mL
    DMSO 40 mM 0.1676 mL 0.8381 mL 1.6762 mL 4.1904 mL
    50 mM 0.1341 mL 0.6705 mL 1.3409 mL 3.3523 mL

    * 該產(chǎn)品常見(jiàn)使用濃度在 mM 級(jí)別；建議您稱取所需的量，然后將其溶解在培養(yǎng)基中，并用 0.22 μm 過(guò)濾器除菌，現(xiàn)用現(xiàn)配。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    3-Methyladenine
    目錄號(hào)：
    HY-19312
    需求量：