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  2. PI3K/Akt/mTOR
  3. Akt
  4. SC79

SC79，一個特異的、能透過血腦屏障的 Akt 激動劑，可激活胞質(zhì)中 Akt，并抑制 Akt 膜轉(zhuǎn)位。SC79 特異性結(jié)合Akt 的PH 結(jié)構(gòu)域。

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SC79 Chemical Structure

SC79 Chemical Structure

CAS No. : 305834-79-1

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2.  同一機構(gòu)（單位）同一產(chǎn)品試用裝僅限申領(lǐng)一次，同一機構(gòu)（單位）一年內(nèi)

     可免費申領(lǐng)三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
1 mg ￥270
In-stock
5 mg ￥600
In-stock
10 mg ￥950
In-stock
25 mg ￥1700
In-stock
50 mg ￥2500
In-stock
100 mg ￥3500
In-stock
200 mg   詢價  
500 mg   詢價  

* Please select Quantity before adding items.

Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 185 篇科研文獻(xiàn)

WB
IF
Cell Viability Assay

    SC79 purchased from MCE. Usage Cited in: Environ Pollut. 2023 Feb 17.

    SC79 induces mitochondria dysfunction and apoptosis in the heart of zebrafish embryos (Fig D and E).

    SC79 purchased from MCE. Usage Cited in: Biopolymers. 2023 Mar 28.

    SC79 (5.0, 7.5, 10 μM; 24 h) significantly induces cell death in T84 cells.

    SC79 purchased from MCE. Usage Cited in: Biopolymers. 2023 Mar 28.

    SC79 (2.5 μM; 4, 6, 24 h) significantly increases the expression of p-AKT and p-AKT/AKT while decreases p-ERK expression and the ratio of p-ERK/ERK ratio in T84 cells.

    SC79 purchased from MCE. Usage Cited in: Toxicology. 2019 May 1;419:32-39.  [Abstract]

    HaCaT cells are treated with SC79 at 10 μM for 0, 0.5, 1, and 2 h, following arecoline treatment of 48 h. Proteins are extracted and used for Western blotting for phosphorylated and total Akt, mTOR, 4E-BP1, and cyclin D1. Akt, mTOR, 4E-BP1 and GAPDH are used as the internal control for p-Akt, p-mTOR, p-4E-BP1 and cyclin D1, respectively.

    SC79 purchased from MCE. Usage Cited in: Mol Med Rep. 2019 May;19(5):4091-4100.  [Abstract]

    Western blot analysis demonstrating the protein levels of LC3, p62, AKT, p-AKT, mTOR and p-mTOR following treatment with Aβ1-42, 3-MA, SC79 and SC79 + RAPA for 48 h, and the expression of the AKT/mTOR signaling pathway?associated proteins of AKT, p-AKT, mTOR and p-mTOR following treatment with Aβ1-42, 3-MA, SC79 and SC79 + RAPA for 48 h.

    SC79 purchased from MCE. Usage Cited in: Acta Biomater. 2018 Nov;81:278-292.  [Abstract]

    PC12 cells are pretreated with 740-YP (30?μM), or SC79 (13.7?μM), or 3BDO (100?μM) for 1?h, and for the duration of GO (50?μg/mL) incubation for 24?h. LC3 turnover is detected by western blotting.

    SC79 purchased from MCE. Usage Cited in: Biomed Pharmacother. 2018 Oct;106:755-762.  [Abstract]

    LTEP-a-2 cells are exposed to SC79 and GSK3β and AKT phosphorylation levels are significantly increased.

    SC79 purchased from MCE. Usage Cited in: Nutrients. 2018 Sep 23;10(10). pii: E1366.  [Abstract]

    Competition tests of SC79, PHT-427, AT7867, and AKT inhibitor VIII with the CGA probe against enriched AKT by CGA-modified functionalized MMs. Bands of AKT are detected by Western blot.

    SC79 purchased from MCE. Usage Cited in: Oncol Rep. 2019 Feb;41(2):811-818.  [Abstract]

    Cells are cultured with and without CAR/SC79 for 24 h, and cell lysates are prepared and analyzed by western blotting to detect the levels of MDM2 and p53Ser15.

    SC79 purchased from MCE. Usage Cited in: Toxicology. 2017 Jul 1;386:72-83  [Abstract]

    Western blot assays and quantitative analysis show that BPDE treatment reduces the expression levels of AKT and eNOS; while simultaneous addition of SC79 significantly increases their expression.

    SC79 purchased from MCE. Usage Cited in: Royal Society of Chemistry. 11th August 2017

    Phillygenin (Phi) modulates phosphorylation of Akt in BEAS-2B cells. BEAS-2B cells are serum-starved overnight and then treated with SC79 and different doses of Phi for 4 h. Thr 308 and Ser 473 phosphorylation of Akt and total Akt are detected by western blot. β-Actin is used as an internal control.

    查看 Akt 亞型特異性產(chǎn)品:

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    SC79, a unique specific and BBB permeable Akt activator, activates Akt in the cytosol and inhibits Akt membrane translocation. SC79 specifically binds to the PH domain of Akt[1][2][3].

    體外研究
    (In Vitro)

    SC79 在 Thr308 和 S473 位點增強 Akt 磷酸化[1]。
    SC79 (10.96 μM) 誘導(dǎo) Akt 的細(xì)胞溶質(zhì)磷酸化。SC79 增強血清饑餓細(xì)胞和富含血清培養(yǎng)基中生長的細(xì)胞中 IGF1 誘導(dǎo)的 Akt 磷酸化[1]。
    SC79 降低神經(jīng)元興奮性毒性并防止中風(fēng)誘導(dǎo)的神經(jīng)元死亡。SC79 抑制 PHAKTM-GFP 質(zhì)膜轉(zhuǎn)位[1]。
    SC79 恢復(fù) BRAT1 敲低細(xì)胞的增殖，并減少 MitoSox 陽性細(xì)胞線粒體中超氧化物的產(chǎn)生[2]。
    SC79 上調(diào) FLIPL/S 表達(dá)，從而抑制 caspase-8 激活[5]。
    注意：
    SC79 在溶液狀態(tài)時不穩(wěn)定[6]，建議使用新拆封的 DMSO 配制，現(xiàn)配現(xiàn)用。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Western Blot Analysis[1]

    Cell Line: HeLa cells.
    Concentration: 4 μg/mL (10.96 μM).
    Incubation Time: 30 min.
    Result: Induced cytosolic phosphorylation of Akt.
    體內(nèi)研究
    (In Vivo)

    SC79 處理，即使在高得多的劑量 (0.4 mg/g) 下，也不會引起小鼠體重、存活率、外觀和行為的任何可檢測變化[1]。
    SC79 (10 mg/kg，ip) 保護(hù) C57BL/6 小鼠免于 fas 誘導(dǎo)的暴發(fā)性肝衰竭[4]。
    SC79 保護(hù)肝細(xì)胞免于 TNFα 介導(dǎo)的細(xì)胞凋亡和小鼠免于 Gal/LPS 誘導(dǎo)的肝損傷和損傷[5]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male, age-matched (6- to 8-weekeold) C57BL/6 or BALB/c mice weighing 16 to 18 g[4].
    Dosage: 10 mg/kg.
    Administration: Intraperitoneally at 0.5 hour before the i.p. administration of an agonistic anti-Fas Jo2 antibody at a lethal dose of 0.5 and 0.4 mg/kg for C57BL/6 and BALB/c mice, respectively.
    Result: Treatment of mice with 10 mg/kg of SC79 at 0.5 hour before Jo2 injection increased mouse survival at 12 hours after Jo2 injection from 0% to 35%, and no additional mortality was observed to the end of the 2-month observation period.
    Animal Model: Male, age-matched (6 to 8 weeks old) C57BL/6 mice weighing 16-18 g[5].
    Dosage: 10 mg/kg.
    Administration: Intraperitoneally at 0.5 h before i.p. administration of 400 mg/kg of D-galactosamine (D-Gal) and 60 μg/kg of lipopolysaccharide (LPS) for C57BL/6 mice.
    Result: Gal/LPS challenge there was more bleeding on the liver of the vehicle control-treated mice as compared to that of SC79-treated mice.
    A single dose of SC79 significantly reduced Gal/LPS-mediated liver damage but not an infiltration of inflammatory cells in liver sections.
    分子量

    364.78

    Formula

    C17H17ClN2O5
    CAS 號
    性狀

    固體

    顏色

    White to off-white

    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式
    Powder -20°C 3 years
    4°C 2 years

    *該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

    溶解性數(shù)據(jù)
    細(xì)胞實驗： 

    DMSO 中的溶解度 : 100 mg/mL (274.14 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    Ethanol 中的溶解度 : 50 mg/mL (137.07 mM; 超聲助溶)

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 2.7414 mL 13.7069 mL 27.4138 mL
    5 mM 0.5483 mL 2.7414 mL 5.4828 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液；該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 10% EtOH    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 5 mg/mL (13.71 mM); 澄清溶液

      此方案可獲得 ≥ 5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 50.0 mg/mL 的澄清 EtOH 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑： 10% EtOH    90% Corn Oil

      Solubility: ≥ 5 mg/mL (13.71 mM); 澄清溶液

      此方案可獲得 ≥ 5 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。

      以 1 mL 工作液為例，取 100 μL 50.0 mg/mL 的澄清 EtOH 儲備液加到 900 μL 玉米油中，混合均勻。

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    請輸入您的動物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
    計算結(jié)果
    工作液所需濃度 : mg/mL
    儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

    *該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。
    請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: ≥98.0%

    參考文獻(xiàn)

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液；該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.7414 mL 13.7069 mL 27.4138 mL 68.5345 mL
    5 mM 0.5483 mL 2.7414 mL 5.4828 mL 13.7069 mL
    10 mM 0.2741 mL 1.3707 mL 2.7414 mL 6.8534 mL
    15 mM 0.1828 mL 0.9138 mL 1.8276 mL 4.5690 mL
    20 mM 0.1371 mL 0.6853 mL 1.3707 mL 3.4267 mL
    25 mM 0.1097 mL 0.5483 mL 1.0966 mL 2.7414 mL
    30 mM 0.0914 mL 0.4569 mL 0.9138 mL 2.2845 mL
    40 mM 0.0685 mL 0.3427 mL 0.6853 mL 1.7134 mL
    50 mM 0.0548 mL 0.2741 mL 0.5483 mL 1.3707 mL
    60 mM 0.0457 mL 0.2284 mL 0.4569 mL 1.1422 mL
    80 mM 0.0343 mL 0.1713 mL 0.3427 mL 0.8567 mL
    100 mM 0.0274 mL 0.1371 mL 0.2741 mL 0.6853 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Inquiry Information

    產(chǎn)品名稱：
    SC79
    目錄號：
    HY-18749
    需求量：