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  2. Epigenetics PI3K/Akt/mTOR Autophagy
  3. AMPK Autophagy Mitophagy
  4. Metformin hydrochloride

Metformin hydrochloride  (Synonyms: 鹽酸二甲雙胍; 1,1-Dimethylbiguanide hydrochloride)

目錄號: HY-17471A 純度: 99.92%
COA 產(chǎn)品使用指南

Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) 抑制肝臟中的線粒體呼吸鏈，導致 AMPK 活化，增強胰島素敏感性，可用于 2 型糖尿病的研究。Metformin hydrochloride 可以透過血腦屏障，誘導自噬 (autophagy)。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務

Metformin hydrochloride Chemical Structure

Metformin hydrochloride Chemical Structure

CAS No. : 1115-70-4

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥550
In-stock
5 mg ￥312
In-stock
10 mg ￥500
In-stock
25 mg ￥800
In-stock
50 mg   詢價  
100 mg   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of Metformin hydrochloride:

MCE 顧客使用本產(chǎn)品發(fā)表的 121 篇科研文獻

WB
IHC
Cell Viability Assay

    Metformin hydrochloride purchased from MCE. Usage Cited in: Redox Biol. 2023 Jun 15, 102786.

    Metformin hydrochloride (Met; 3, 6?mM; 30 min) inhibits MGO-induced the increased expression of Bax and caspase-3 cleavage and decreased expression of Bcl-2 in ARPE-19 cells.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Redox Biol. 2023 Jun 15, 102786.

    Metformin hydrochloride (Metformin; 2, 6, 20 mM; 30 min) attenuates MGO-induced cell death in a concentration-dependent manner in ARPE-19?cells.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Endocrinology. 2018 May 1;159(5):2008-2021.  [Abstract]

    Male C57BL/6 mice are intragastrically treated with Metformin or placebo at 3 mg/kg/day for 10 weeks in the presence of HFD after 10-week-HFD feeding and then sacrificed for sample collection. Western blot analysis of AMPKα1, AMPK, p-AMPK, SREBP1 and FAS.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Cell Proteomics. 2017 Jul;16(7):1324-1334.  [Abstract]

    To further validate the MS quantification results, Western blot analysis is carried out using an anti-H3K36 dimethylation antibody. Consistent with the mass spectrometric data, H3K36 dimethylation was elevated in DIO mouse livers, whereas Metformin treatment can significantly decrease histone H3K36 dimethylation in DIO mice to a level close to that of the chow-fed control mice.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Oncol. 2017 Aug;11(8):1035-1049.  [Abstract]

    Co-treatment of Metformin and Ribociclib induces cell death in Hep3B cells. Cells are exposed to Ribociclib at 25 μM and/or Metformin at 10 mM for 72 h.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Oncol. 2017 Oct;11(10):1475-1492.  [Abstract]

    (A) IHC staining of N-cadherin and E-cadherin in the pancreas from KPC mice treated with vehicle, Metformin, or KRIBB11. (B) Masson's trichrome staining and IHC staining of α-SMA in pancreatic tissues from mice treated with vehicle, Metformin, or KRIBB11.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Oncol. 2017 Oct;11(10):1475-1492.  [Abstract]

    MiaPaCa-2 cells are treated with a gradient concentration of Metformin for 24 h, and then, western blotting is performed to show the activation of p-AMPK and the suppression of HSF1 and HSP70.

    Metformin hydrochloride purchased from MCE. Usage Cited in: J Cell Mol Med. 2017 Dec;21(12):3322-3336.  [Abstract]

    Metformin prevents the loss of tight junction (TJ) proteins after SCI. Representative Western blots and quantification data of Occludin, Claudin-5, ZO-1 and β-actin at 3 day after injury.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Biochem Pharmacol. 2017 Aug 15;138:49-60.  [Abstract]

    PP5 overexpression suppresses AMPK-Thr172 phosphorylation induced by AMPK activators, AICAR and metformin. Hep3B cells are transfected with indicated plasmids and treated with AICAR (2 mM; 3 h) or Metformin (3 mM; 16 h) to simulate AMPK phosphorylation.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Mol Neurobiol. 2017 Jul;54(5):3327-3341.  [Abstract]

    Met attenuates apoptosis caused by traumatic spinal cord injury in rat. a, b Representative western blots and quantification data of cleaved caspase 3 and β-actin in each group rats. c, d Representative western blots and quantification data of Bax, Bcl-2, and β-actin in each group rats.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2017;44(4):1381-1395.  [Abstract]

    U87 cells are treated with Met alone or in the presence of isogambogenic acid for 24 h. The expression levels of phosphorylated AMPK, mTOR and 4E-BP1 are assessed by western blotting, with total AMPK, mTOR and 4E-BP1 used as the internal controls.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Front Immunol. 2016 Dec 12;7:597.  [Abstract]

    Metformin increases adenosine triphosphate-induced inflammasome activation in bone marrow-derived macrophages. (A) Cells are treated. Indicated protein levels in both cell lysates and supernatants are evaluated by western blotting. β-Tubulin is used as a loading control for cell lysates. (B–D) The quantitative analyses of the active caspase-1p10 (B), mature interleukin-1β (C), and HMGB1 (D) levels in the supernatants (A) are shown.

    Metformin hydrochloride purchased from MCE. Usage Cited in: Front Pharmacol. 2016 Oct 20;7:390.  [Abstract]

    Metformin, an AMPK agonist, counteracts the effect of Piperine on suppressing ATP-induced AMPK activation and inflammatory mediator release. J774A.1 cells are pre-treated with 80 μM Piperine and BMDMs are pre-treated with 160 μM Piperine for 4 h. Without being washed out of Piperine, these cells are primed with LPS (500 ng/mL) for 4 h. Next, the cells are treated with Metformin (1 mM) for 1 h (in the absence of Piperine and LPS). Finally, in the presence of Metformin, the BMDMs are stimulated wi

    • 生物活性

    • 實驗參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) inhibits the mitochondrial respiratory chain in the liver, leading to activation of AMPK, enhancing insulin sensitivity for type 2 diabetes research. Metformin hydrochloride triggers autophagy[1].

    IC50 & Target[2]

    AMPK

     

    細胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    A549 IC50
    208 μM
    Compound: Metformin
    Antiproliferative activity against human A549 cells by IncuCyte live-cell imaging assay
    Antiproliferative activity against human A549 cells by IncuCyte live-cell imaging assay
    		[PMID: 33103432]
    HEK293 IC50
    1230 μM
    Compound: Metformin
    Inhibition of human OCT1 expressed in HEK293 cells assessed as decrease in uptake of MPP+ after 1 min
    Inhibition of human OCT1 expressed in HEK293 cells assessed as decrease in uptake of MPP+ after 1 min
    		[PMID: 28230985]
    HEK293 IC50
    250 μM
    Compound: metformin
    Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay
    Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay
    		[PMID: 23241029]
    HepG2 EC50
    0.27 μM
    Compound: Metformin
    Increase in glucose consumption in insulin-resistant human HepG2 cells after 24 hrs
    Increase in glucose consumption in insulin-resistant human HepG2 cells after 24 hrs
    		[PMID: 21856048]
    IGROV-1 IC50
    850 μM
    Compound: Metformin (2)
    Antiproliferative activity against human IGROV-1 cells incubated for 72 hrs by MTT assay
    Antiproliferative activity against human IGROV-1 cells incubated for 72 hrs by MTT assay
    		[PMID: 33857729]
    MCF7 IC50
    9.9 nM
    Compound: 8
    Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
    Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
    		[PMID: 33479633]
    PC-3 IC50
    189 nM
    Compound: 8
    Cytotoxicity against human PC-3 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
    Cytotoxicity against human PC-3 cells assessed as inhibition of cell growth measured after 72 hrs by MTT assay
    		[PMID: 33479633]
    SK-OV-3 IC50
    150 μM
    Compound: Metformin (2)
    Antiproliferative activity against human SK-OV-3 cells incubated for 72 hrs by MTT assay
    Antiproliferative activity against human SK-OV-3 cells incubated for 72 hrs by MTT assay
    		[PMID: 33857729]
    體外研究
    (In Vitro)

    Metformin hydrochloride (1,1-Dimethylbiguanide hydrochloride) 以濃度依賴性方式抑制 ESC 增殖。A-ESC 的 IC50 為 2.45 mM，N-ESC 的 IC50 為 7.87 mM。Metformin 對分泌期 A-ESC 中 AMPK 信號傳導的激活作用比對增殖期細胞的作用更明顯[2]。
    Metformin (0-500 μM) hydrochloride 以劑量依賴性方式降低糖原合成，在培養(yǎng)的大鼠肝細胞中的 IC50 值為 196.5 μM[3]。
    Metformin hydrochloride 對 PC-3 細胞表現(xiàn)出細胞活力和細胞毒性作用，IC50 為 5 mM[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內研究
    (In Vivo)

    Metformin hydrochloride（1,1-Dimethylbiguanide hydrochloride；100 mg/kg，口服）單獨使用以及 Metformin（25、50、100 mg/kg）與 NSC 37745 組通過組織病理學分析減輕了肌細胞壞死[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    165.62

    Formula

    C4H12ClN5
    CAS 號
    性狀

    固體

    顏色

    White to off-white

    中文名稱

    鹽酸二甲雙胍；甲福明；降糖片
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性數(shù)據(jù)
    細胞實驗： 

    H2O 中的溶解度 : ≥ 100 mg/mL (603.79 mM)

    DMSO 中的溶解度 : ≥ 1.7 mg/mL (10.26 mM; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

    * "≥" means soluble, but saturation unknown.

    配制儲備液
    濃度 溶劑體積 質量 1 mg 5 mg 10 mg
    1 mM 6.0379 mL 30.1896 mL 60.3792 mL
    5 mM 1.2076 mL 6.0379 mL 12.0758 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時，請在6個月內使用，-20°C儲存時，請在1個月內使用。

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗：

    請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
    ——為保證實驗結果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑： 5% DMSO    40% PEG300    5% Tween-80    50% Saline

      Solubility: ≥ 3 mg/mL (18.11 mM); 澄清溶液

    • 方案 二

      請依序添加每種溶劑： 5% DMSO    95% (20% SBE-β-CD in Saline)

      Solubility: ≥ 3 mg/mL (18.11 mM); 澄清溶液

    以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請依序添加每種溶劑： PBS

      Solubility: 100 mg/mL (603.79 mM); 澄清溶液; 超聲助溶

    動物溶解方案計算器
    請輸入動物實驗的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實驗過程有損耗，建議您多配一只動物的量
    計算結果
    工作液所需濃度 : mg/mL
    該產(chǎn)品水溶性佳，請具體參考實測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
    您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，如有需要，請與 MCE 中國技術支持聯(lián)系。
    純度 & 產(chǎn)品資料

    純度: 99.92%

    參考文獻
    Cell Assay
    [2]

    ESCs are plated in 96-well plates at a concentration of 1×103cells/well. After attachment, cells are treated with different doses of metformin/compound C for 0 min, 15 min, 1 h, and 24 h. MTT assays are performed as described previously. In brief, MTT (5 mg/mL) is added to the 96-well plates at a volume of 10 μL/well, and the plates are incubated for 4 h. The MTT reaction is terminated by removal of the culture medium containing MTT, and 100 μL DMSO per well are added and incubated at RT on a shaker for 10 min to ensure that the crystals had dissolved sufficiently. Absorbance values are measured at 595 nm. Cell proliferation (percentage of control) is calculated as follows: absorbance (experimental group)/absorbance (control group). Cell proliferation inhibition (percentage of control) is calculated as follows: 100%?cell proliferation (percentage of control). Each experiment is performed in duplicate and repeated six times to assess result consistency.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    The animals are randomized into six groups consisting of six rats each. Rats in group 1 (control) receives a subcutaneous injection of physiological saline (0.5 mL) and are left untreated for the entire experimental period. Rats in group 2 receives an oral administration of metformin (100 mg/kg; twice daily) for 2 days and are subcutaneously injected with saline at an interval of 24 h for 2 consecutive days. Rats in group 3 (MI control) receives an oral administration of saline (twice daily) for 2 days and are sc injected with NSC 37745 (100 mg/kg) daily for 2 consecutive days at an interval of 24 h. Rats in groups 4 to 6 are treated with metformin at 25, 50, and 100 mg/kg. Metformin is dissolved in saline and is gavaged at a volume of 0.25-0.5 mL twice a day at an interval of 12 h, started immediately before NSC 37745 injection.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時，請在6個月內使用，-20°C儲存時，請在1個月內使用。

    可選溶劑 濃度 溶劑體積 質量 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 6.0379 mL 30.1896 mL 60.3792 mL 150.9480 mL
    5 mM 1.2076 mL 6.0379 mL 12.0758 mL 30.1896 mL
    10 mM 0.6038 mL 3.0190 mL 6.0379 mL 15.0948 mL
    H2O 15 mM 0.4025 mL 2.0126 mL 4.0253 mL 10.0632 mL
    20 mM 0.3019 mL 1.5095 mL 3.0190 mL 7.5474 mL
    25 mM 0.2415 mL 1.2076 mL 2.4152 mL 6.0379 mL
    30 mM 0.2013 mL 1.0063 mL 2.0126 mL 5.0316 mL
    40 mM 0.1509 mL 0.7547 mL 1.5095 mL 3.7737 mL
    50 mM 0.1208 mL 0.6038 mL 1.2076 mL 3.0190 mL
    60 mM 0.1006 mL 0.5032 mL 1.0063 mL 2.5158 mL
    80 mM 0.0755 mL 0.3774 mL 0.7547 mL 1.8868 mL
    100 mM 0.0604 mL 0.3019 mL 0.6038 mL 1.5095 mL

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Metformin hydrochloride
    目錄號：
    HY-17471A
    需求量：