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  2. Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease Anti-infection
  3. VD/VDR Endogenous Metabolite Bacterial
  4. Vitamin D3

Vitamin D3  (Synonyms: 維生素D3; Cholecalciferol; Colecalciferol)

目錄號: HY-15398 純度: 99.94%
COA 產(chǎn)品使用指南

Vitamin D3 (Cholecalciferol; Colecalciferol) 是維生素 D 的天然存在形式，代謝激活后能誘導細胞分化和癌細胞增殖。

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Vitamin D3 Chemical Structure

Vitamin D3 Chemical Structure

CAS No. : 67-97-0

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規(guī)格 價格 是否有貨 數(shù)量
100 mg ￥550
In-stock
1 g ￥1200
In-stock
5 g ￥2400
In-stock
10 g   詢價  
50 g   詢價  

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Customer Review

Other Forms of Vitamin D3:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Vitamin D3 (Cholecalciferol; Colecalciferol) is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation of cancer cells.

IC50 & Target

Human Endogenous Metabolite

 

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
C3H 10T1/2 IC50
2.9 μM
Compound: 3, VD3
Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Ptch mRNA expression after 24 hrs by RT-PCR analysis
Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Ptch mRNA expression after 24 hrs by RT-PCR analysis
[PMID: 24900716]
C3H 10T1/2 IC50
2.9 μM
Compound: 3, VD3, Vitamin D3
Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis
Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis
[PMID: 24730984]
C3H 10T1/2 IC50
4.1 μM
Compound: 3, VD3
Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Gli1 mRNA expression after 24 hrs by RT-PCR analysis
Inhibition of Hedgehog signaling in mouse C3H10T1/2 cells assessed as downregulation of Gli1 mRNA expression after 24 hrs by RT-PCR analysis
[PMID: 24900716]
C3H 10T1/2 IC50
4.1 μM
Compound: 3, VD3, Vitamin D3
Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis
Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in mouse C3H10T1/2 cells after 24 hrs by RT-PCR analysis
[PMID: 24730984]
C3H 10T1/2 IC50
4.1 μM
Compound: 2, VD3, vitamin D3
Inhibition of hedgehog signaling in mouse C3H10T1/2 cells assessed as reduction in Gli1 mRNA level after 24 hrs by quantitative PCR analysis
Inhibition of hedgehog signaling in mouse C3H10T1/2 cells assessed as reduction in Gli1 mRNA level after 24 hrs by quantitative PCR analysis
[PMID: 25676864]
Daoy IC50
> 10 μM
Compound: 3, VD3, Vitamin D3
Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis
Inhibition of hedgehog signaling pathway-mediated GLI1 mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis
[PMID: 24730984]
Daoy IC50
> 10 μM
Compound: 3, VD3, Vitamin D3
Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis
Inhibition of hedgehog signaling pathway-mediated PTCH mRNA expression in human DaOY cells after 24 hrs by RT-PCR analysis
[PMID: 24730984]
Daoy IC50
> 10 μM
Compound: 3, VD3
Inhibition of Hedgehog signaling in human DaOY cells assessed as downregulation of Gli1 mRNA expression after 48 hrs by RT-PCR analysis
Inhibition of Hedgehog signaling in human DaOY cells assessed as downregulation of Gli1 mRNA expression after 48 hrs by RT-PCR analysis
[PMID: 24900716]
HEK293 EC50
0.21 nM
Compound: VitD3
Agonist activity at human VDR expressed in HEK293 cells by luciferase reporter gene assay
Agonist activity at human VDR expressed in HEK293 cells by luciferase reporter gene assay
[PMID: 25305688]
HT-29 GI50
53.8 μM
Compound: 2, VD3
Growth inhibition of human HT-29 cells after 72 hrs by MTS-PMS assay
Growth inhibition of human HT-29 cells after 72 hrs by MTS-PMS assay
[PMID: 23972439]
HT-29 GI50
68.5 μM
Compound: VD3
Growth inhibition of human HT-29 cells overexpressing VDR gene after 72 hrs by MTS assay
Growth inhibition of human HT-29 cells overexpressing VDR gene after 72 hrs by MTS assay
[PMID: 22687748]
Medulloblastoma cell GI50
5.8 μM
Compound: 1; VD3
Antiproliferative activity against medulloblastoma cells derived from conditional patched knockout mouse after 48 hrs by [3H]-thymidine incorporation assay
Antiproliferative activity against medulloblastoma cells derived from conditional patched knockout mouse after 48 hrs by [3H]-thymidine incorporation assay
[PMID: 30471551]
U-87MG ATCC GI50
23.2 μM
Compound: VD3
Growth inhibition of human U87MG cells after 72 hrs by MTS assay
Growth inhibition of human U87MG cells after 72 hrs by MTS assay
[PMID: 22687748]
U-87MG ATCC IC50
29.7 μM
Compound: VD3
Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay
Antiproliferative activity against human U87MG cells after 72 hrs by MTS assay
[PMID: 22226657]
體外研究
(In Vitro)

Vitamin D3 是一種在體內(nèi)無活性的維生素 D 分子。Vitamin D3 需要經(jīng)歷兩次羥基化過程才能被激活。Vitamin D3 首先在肝臟中被羥基化，形成循環(huán)激素原 25-羥基維生素 D3 [25(OH)D3]，其作用機理是酶 25-羥化酶 (CYP27A1)，也可能是其他酶（例如 CYP2R1）[1]。
第二次羥基化發(fā)生在腎臟中，通過酶 1-α-羥化酶，生成 1,25-二羥基膽鈣化醇（骨化三醇），這是 Vitamin D3 的生物活性形式[1]。
Vitamin D3（2-10 μM；24-48 小時）表現(xiàn)出劑量和時間依賴性的抗增殖作用。用 10 μM Vitamin D3 處理 72 小時后，細胞活力最大降低 62% (IK)、52% (RL-95-2) 和 55% (Hec-1A)。但 24 小時的暴露并未顯著減少活細胞[2]。
Vitamin D3 (10 μM；24-48 小時) 顯示核 VDR 染色顯著增加，并在 IK 細胞中產(chǎn)生局部 VDR 活化[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: EC cell lines from EEC, Ishikawa 3-H-12(IK), RL-95/2, and HEC-1-A cells
Concentration: 2-10 μM
Incubation Time: 24-72 hours
Result: Reduced viability in response to VD3 in a dose- and time-dependent manner.
Indicated that the conversion of VD3 to 25(OH)D is an essential step for the reduced cell viability effect.

Cell Viability Assay[2]

Cell Line: EC cell lines from EEC, Ishikawa 3-H-12(IK) cells
Concentration: 10 μM
Incubation Time: 24-48 hours
Result: Improved nuclear VDR content in IK cells.
體內(nèi)研究
(In Vivo)

Vitamin D3（口服管飼；5 mg/kg；7 天）僅增強肝臟中的 CCl4 毒性，如血漿 ALT 和 AST 水平（肝損傷的生化標志物）所示。它顯著增加小鼠的腎鈣水平，但小鼠之間的腎鈣含量沒有顯著差異[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ddY mice on CCl4?toxicity[3]
Dosage: 5 mg/kg
Administration: Oral gavage; 5 mg/kg; 7 days
Result: Potentiated CCl4-induced hepatotoxicity and enhanced mouse mortality, without increasing renal toxicity and generation of liver fibrosis.
Clinical Trial
分子量

384.64

Formula

C27H44O
CAS 號
性狀

固體

顏色

White to off-white

中文名稱

維生素D3；膽骨化醇
結構分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

4°C, protect from light, stored under nitrogen

*該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : 10 mg/mL (26.00 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質量 1 mg 5 mg 10 mg
1 mM 2.5998 mL 12.9992 mL 25.9983 mL
5 mM 0.5200 mL 2.5998 mL 5.1997 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液；該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (5.41 mM); 懸濁液; 超聲助溶

    此方案可獲得 2.08 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (2.60 mM); 澄清溶液

    此方案可獲得 ≥ 1 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 10.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

*該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.94%

參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度，選擇合適的溶劑配制儲備液；該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

可選溶劑 濃度 溶劑體積 質量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5998 mL 12.9992 mL 25.9983 mL 64.9958 mL
5 mM 0.5200 mL 2.5998 mL 5.1997 mL 12.9992 mL
10 mM 0.2600 mL 1.2999 mL 2.5998 mL 6.4996 mL
15 mM 0.1733 mL 0.8666 mL 1.7332 mL 4.3331 mL
20 mM 0.1300 mL 0.6500 mL 1.2999 mL 3.2498 mL
25 mM 0.1040 mL 0.5200 mL 1.0399 mL 2.5998 mL
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產(chǎn)品名稱：
Vitamin D3
目錄號：
HY-15398
需求量：