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  2. JAK/STAT Signaling Stem Cell/Wnt
  3. STAT
  4. Homoharringtonine

Homoharringtonine  (Synonyms: 高三尖杉酯堿; Omacetaxine mepesuccinate; HHT)

目錄號(hào): HY-14944 純度: 99.75%
COA 產(chǎn)品使用指南

Homoharringtonine (Omacetaxine mepesuccinate;HHT) 是具有抗腫瘤特性的細(xì)胞毒性生物堿，其通過(guò)抑制翻譯延長(zhǎng)來(lái)起作用。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Homoharringtonine Chemical Structure

Homoharringtonine Chemical Structure

CAS No. : 26833-87-4

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥840
In-stock
5 mg ￥700
In-stock
10 mg ￥865
In-stock
50 mg ￥2400
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100 mg   詢價(jià)  
200 mg   詢價(jià)  

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Customer Review

Other Forms of Homoharringtonine:

    Homoharringtonine purchased from MCE. Usage Cited in: Oncotarget. 2017 Jun 6;8(23):37594-37604.  [Abstract]

    The expression of apoptosis proteins. K562/G01 cells treated with HHT 0, 50nM, 100 nM, 200nM for 24h. Bcl-2, caspase9, caspase3 express.

    查看 STAT 亞型特異性產(chǎn)品:

    • 生物活性

    • 實(shí)驗(yàn)參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Homoharringtonine (Omacetaxine mepesuccinate;HHT) is a cytotoxic alkaloid with antitumor properties which acts by inhibiting translation elongation.

    IC50 & Target[1]

    STAT3

     

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    4T1 IC50
    0.06 μM
    Compound: 1; HHT
    Antiproliferative activity against mouse 4T1 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against mouse 4T1 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    A204 IC50
    0.004 μM
    Compound: 1; HHT
    Antiproliferative activity against human A204 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human A204 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    A549 IC50
    0.03 μM
    Compound: Homoharringtonine
    Cytotoxicity against human A549 cells assessed as reduction of cell growth by SRB method
    Cytotoxicity against human A549 cells assessed as reduction of cell growth by SRB method
    		[PMID: 18782668]
    A549 IC50
    18.28 nM
    Compound: HHT
    Inhibition of Nuclear factor erythroid 2-related factor 2 5'-untranslated region guanine-rich DNA sequence in human A549 cells harboring ARE-GFP-Luc assessed as reduction in ARE-luciferase activity after 24 hrs by firefly luciferase reporter gene assay
    Inhibition of Nuclear factor erythroid 2-related factor 2 5'-untranslated region guanine-rich DNA sequence in human A549 cells harboring ARE-GFP-Luc assessed as reduction in ARE-luciferase activity after 24 hrs by firefly luciferase reporter gene assay
    		[PMID: 31270017]
    A549 IC50
    31.6 nM
    Compound: homoharringtonine
    Cytotoxicity against human A549 cells after 48 hrs by MTT assay
    Cytotoxicity against human A549 cells after 48 hrs by MTT assay
    		[PMID: 31916761]
    BaF3 IC50
    0.081 μM
    Compound: 1; HHT
    Antiproliferative activity against mouse BaF3 cells expressing Bcr/Abl-T315I mutant assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against mouse BaF3 cells expressing Bcr/Abl-T315I mutant assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    Caco-2 CC50
    1.72 μM
    Compound: HOMOHARRINGTONINE
    Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
    Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
    		10.21203/rs.3.rs-23951/v1
    Caco-2 IC50
    4.71 μM
    Compound: HOMOHARRINGTONINE
    Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
    Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
    		10.21203/rs.3.rs-23951/v1
    HCT-116 IC50
    0.013 μM
    Compound: 1; HHT
    Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    HeLa IC50
    0.4 μM
    Compound: Homoharringtonine
    Cytotoxicity against human HeLa cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
    Cytotoxicity against human HeLa cells assessed as inhibition of cell proliferation incubated for 48 hrs by MTT assay
    		[PMID: 33905250]
    HeLa IC50
    1.7 μM
    Compound: HHT
    Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay
    Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay
    		[PMID: 35148094]
    HepG2 IC50
    34.5 nM
    Compound: homoharringtonine
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    		[PMID: 31916761]
    HL-60 IC50
    0.013 μM
    Compound: 1; HHT
    Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    HL-60 IC50
    0.02 μM
    Compound: homoharringtonine
    Cytotoxicity against human HL60 cells assessed as growth inhibition after 72 hrs by Alamar Blue assay
    Cytotoxicity against human HL60 cells assessed as growth inhibition after 72 hrs by Alamar Blue assay
    		10.1039/C0MD00241K
    HL-60 IC50
    0.022 μM
    Compound: HHT
    Antiproliferative activity against human HL60 cells after 62 hrs by MTS assay
    Antiproliferative activity against human HL60 cells after 62 hrs by MTS assay
    		[PMID: 29286665]
    HL-60 IC50
    21.8 nM
    Compound: HHT
    Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay
    Antiproliferative activity against human HL-60 cells assessed as inhibition of cell growth measured after 68 hrs by MTS assay
    		[PMID: 35148094]
    Huh-7 IC50
    0.04 μM
    Compound: 1; HHT
    Antiproliferative activity against human Huh-7 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human Huh-7 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    K562 IC50
    0.014 μM
    Compound: 1; HHT
    Antiproliferative activity against human K562 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human K562 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    KB IC50
    0.004 μM
    Compound: Homoharringtonine
    Cytotoxicity against human KB cells assessed as reduction of cell growth by SRB method
    Cytotoxicity against human KB cells assessed as reduction of cell growth by SRB method
    		[PMID: 18782668]
    L02 IC50
    0.064 μM
    Compound: 1; HHT
    Cytotoxicity against human HL7702 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Cytotoxicity against human HL7702 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    MDA-MB-453 IC50
    0.021 μM
    Compound: 1; HHT
    Antiproliferative activity against human MDA-MB-453 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human MDA-MB-453 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    MOLT-4 IC50
    0.007 μM
    Compound: 1; HHT
    Antiproliferative activity against human MOLT-4 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human MOLT-4 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    MV4-11 IC50
    0.005 μM
    Compound: 1; HHT
    Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    NCI-H1299 IC50
    0.022 μM
    Compound: 1; HHT
    Antiproliferative activity against human NCI-H1299 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human NCI-H1299 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    NCI-H1975 IC50
    0.022 μM
    Compound: 1; HHT
    Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    NCI-H716 IC50
    0.065 μM
    Compound: 1; HHT
    Antiproliferative activity against human NCI-H716 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human NCI-H716 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    OCI-Ly3 IC50
    0.005 μM
    Compound: 1; HHT
    Antiproliferative activity against human OCILY3 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human OCILY3 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    U-937 IC50
    0.012 μM
    Compound: 1; HHT
    Antiproliferative activity against human U-937 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    Antiproliferative activity against human U-937 cells assessed as inhibition of cell growth measured after 72 hrs by CCK8 assay
    		[PMID: 36242991]
    體外研究
    (In Vitro)

    Homoharringtonine 以劑量和時(shí)間依賴性方式抑制 IL-6 誘導(dǎo)的 STAT3 磷酸化。Homoharringtonine (HHT) 抑制細(xì)胞生長(zhǎng)、細(xì)胞活力和集落形成，并通過(guò)線粒體途徑誘導(dǎo)細(xì)胞凋亡。研究了 Homoharringtonine 對(duì)人 NSCLC 細(xì)胞系的細(xì)胞毒性，A549 (野生型 EGFR) 和 NCI-H1975 (H1975，具有 L858R 和 T790M 的突變型 EGFR)，吉非替尼用作對(duì)照。MTT 法檢測(cè)，Homoharringtonine 對(duì) A549 具有中度細(xì)胞毒性，IC50 為 3.7 μM，H1975 細(xì)胞對(duì) Homoharringtonine 更敏感，IC50 為 0.7 μM。通過(guò) MTT 測(cè)定，Homoharringtonine 以時(shí)間和劑量依賴性方式抑制 A549 細(xì)胞和 H1975 細(xì)胞的細(xì)胞增殖和生長(zhǎng)。通過(guò)臺(tái)盼藍(lán)排斥試驗(yàn)，Homoharringtonine 以劑量和時(shí)間依賴的方式快速減少活的 A549 和 H1975 細(xì)胞。Homoharringtonine 顯著抑制 A549 和 H1975 細(xì)胞的克隆形成能力[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    與載體對(duì)照或吉非替尼相比，Homoharringtonine (10 mg/kg) 可有效抑制腫瘤生長(zhǎng) (P<0.05)。此外，Homoharringtonine (HHT) 處理不會(huì)減輕小鼠體重，這表明 Homoharringtonine 沒(méi)有明顯的副作用。對(duì)所有小鼠實(shí)施安樂(lè)死，分離腫瘤并成像，收集腫瘤樣本細(xì)胞以提取蛋白質(zhì)，以通過(guò)蛋白質(zhì)印跡確定 Homoharringtonine 是否抑制 STAT3 磷酸化。與載體對(duì)照或吉非替尼處理相比，Homoharringtonine 處理組的 STAT3 磷酸化和 MCL1 水平顯著降低。同時(shí)，與上述結(jié)果一致，AKT1/2/3 和 ERK1/2 磷酸化未被 Homoharringtonine 處理抑制。為了進(jìn)一步檢查經(jīng)過(guò)不同處理的異種移植腫瘤樣本中的 STAT3 磷酸化，將腫瘤樣本冷凍并切成 10 μM 的切片用于熒光免疫組織化學(xué)。與媒介物對(duì)照或吉非替尼處理相比，Homoharringtonine 處理抑制 STAT3 磷酸化[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    分子量

    545.62

    Formula

    C29H39NO9
    CAS 號(hào)
    性狀

    固體

    顏色

    Off-white to yellow

    中文名稱

    高三尖杉酯堿
    結(jié)構(gòu)分類
    初始來(lái)源
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式

    4°C, protect from light

    *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    DMSO 中的溶解度 : ≥ 50 mg/mL (91.64 mM; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

    H2O 中的溶解度 : 1.4 mg/mL (2.57 mM; 超聲助溶)

    * "≥" means soluble, but saturation unknown.

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 1.8328 mL 9.1639 mL 18.3278 mL
    5 mM 0.3666 mL 1.8328 mL 3.6656 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (protect from light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    * 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

    以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
    ——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

    • 方案 一

      請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.58 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (4.58 mM); 澄清溶液

      此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

      以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

      2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

    以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶。

    • 方案 一

      請(qǐng)依序添加每種溶劑： PBS

      Solubility: 1.67 mg/mL (3.06 mM); 澄清溶液; 超聲助溶 (<60°C)

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量
    請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過(guò) 2%。
    方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購(gòu)。 ，Tween 80，均可在 MCE 網(wǎng)站選購(gòu)。
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

    *In solvent : -80°C, 1 year; -20°C, 6 months (protect from light)

    您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
    動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
    連續(xù)給藥周期超過(guò)半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
    請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
    純度 & 產(chǎn)品資料

    純度: 99.96%

    參考文獻(xiàn)
    Cell Assay
    [1]

    Human NSCLC cell lines MCF-10A, A549 and H1975 cells are seeded into 96-well plate and precultured for 24 h, then treated with Homoharringtonine for 24 h or 48 h. Cell cytotoxicity is determined by MTT assay. The absorbance is measured at 570 nm by Varioskan Flash Multimode Reader, and the cell death rate is calculated. Cell viability is estimated by trypan blue dye exclusion assay. The cells which exclude the dye are viable. Place 0.5 mL of a suitable cell suspension (dilute cells in complete medium without serum to 1×106 cells per mL) following adding 0.1 mL of 0.4% trypan blue dye and mixing thoroughly, and then incubate at room temperature for 3 min and load into a hemacytometer to count cells in three separate fields (nonviable, deep blue cells as well as viable, clear cells). The cell viability rate is calculated. After staining with Hoechst 33258 at 10 mg/mL for 10 min, cell death is observed by a fluorescence microscope[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    Mice[1] Equal amounts of female and male nude immunodeficient mice (nu/nu), 6-8 weeks old, are injected subcutaneously with NSCLC H1975 cells (2.5×106) suspended in 100 μL RPMI-1640 medium into the rightflank of each mouse. Treatments are started when the tumors reached a palpablesize. Mice are randomly divided into three groups (n=10) and treated with Homoharringtonine (10 mg/kg), Gefitinib (30 mg/kg) or vehicle control for 3 weeks. Vernier caliper measurements of the longest perpendicular tumor diameters are conducted along with the mice treatment to estimate the tumor volume, using the following formula: 4π/3×(width/2)2×(length/2), representing the 3-dimensional volume of an ellipse tumor tissue. Animals are sacrificed when tumors reached to 2 cm or if the mice appeared moribund to prevent unnecessary morbidity to the mice. At the time of the animals’ death, tumors are excised; cells are separatedand lyzed for western blot using anti-STAT3 antibody, anti-pSTAT3, anti-MCL1 and anti-GAPDH antibodies and immunohistochemistry.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (protect from light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.8328 mL 9.1639 mL 18.3278 mL 45.8194 mL
    DMSO 5 mM 0.3666 mL 1.8328 mL 3.6656 mL 9.1639 mL
    10 mM 0.1833 mL 0.9164 mL 1.8328 mL 4.5819 mL
    15 mM 0.1222 mL 0.6109 mL 1.2219 mL 3.0546 mL
    20 mM 0.0916 mL 0.4582 mL 0.9164 mL 2.2910 mL
    25 mM 0.0733 mL 0.3666 mL 0.7331 mL 1.8328 mL
    30 mM 0.0611 mL 0.3055 mL 0.6109 mL 1.5273 mL
    40 mM 0.0458 mL 0.2291 mL 0.4582 mL 1.1455 mL
    50 mM 0.0367 mL 0.1833 mL 0.3666 mL 0.9164 mL
    60 mM 0.0305 mL 0.1527 mL 0.3055 mL 0.7637 mL
    80 mM 0.0229 mL 0.1145 mL 0.2291 mL 0.5727 mL

    * 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱：
    Homoharringtonine
    目錄號(hào)：
    HY-14944
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