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  2. Vitamin D Related/Nuclear Receptor Metabolic Enzyme/Protease
  3. Androgen Receptor Endogenous Metabolite
  4. DHEA

DHEA  (Synonyms: 脫氫表雄酮; Prasterone; Dehydroisoandrosterone; Dehydroepiandrosterone)

目錄號: HY-14650 純度: 99.93%
COA 產(chǎn)品使用指南 技術支持

DHEA (Prasterone) 是最豐富的類固醇激素之一。 DHEA通過多種信號傳導途徑介導其作用，并通過轉(zhuǎn)化成雄激素和雌激素衍生物（例如，雄激素，雌激素，7α和7β DHEA (Prasterone)，以及7α和7β表雄甾酮衍生物）通過其特異性受體起作用。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務

DHEA Chemical Structure

DHEA Chemical Structure

CAS No. : 53-43-0

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥550
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100 mg ￥500
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250 mg ￥1000
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500 mg ￥1600
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5 g   詢價  

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Customer Review

Other Forms of DHEA:

MCE 顧客使用本產(chǎn)品發(fā)表的 29 篇科研文獻

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

DHEA (Prasterone) is one of the most abundant steroid hormones. DHEA (Prasterone) mediates its action via multiple signaling pathways involving specific membrane receptors and via transformation into androgen and estrogen derivatives (e.g., androgens, estrogens, 7α and 7β DHEA, and 7α and 7β epiandrosterone derivatives) acting through their specific receptors.

IC50 & Target

Human Endogenous Metabolite

 

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
A-431 IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human A431 cells after 24 hrs by MTT assay
Cytotoxic activity against human A431 cells after 24 hrs by MTT assay
[PMID: 25456391]
A-431 IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human A431 cells after 48 hrs by MTT assay
Cytotoxic activity against human A431 cells after 48 hrs by MTT assay
[PMID: 25456391]
A549 IC50
> 20 μM
Compound: c1
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
[PMID: 30822712]
A549 IC50
47.8 μM
Compound: DHEA
Cytotoxic activity against human A549 cells after 24 hrs by MTT assay
Cytotoxic activity against human A549 cells after 24 hrs by MTT assay
[PMID: 25456391]
A549 IC50
47.8 μM
Compound: DHEA
Cytotoxic activity against human A549 cells after 48 hrs by MTT assay
Cytotoxic activity against human A549 cells after 48 hrs by MTT assay
[PMID: 25456391]
CHO EC50
3.33 μM
Compound: 49
Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
Agonist activity at human TGR5 expressed in CHO cells by luciferase assay
[PMID: 18307294]
DU-145 IC50
> 50 μM
Compound: DHEA
Antiproliferative activity against human DU145 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
Antiproliferative activity against human DU145 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
[PMID: 26866967]
HCT-116 IC50
> 50 μM
Compound: DHEA
Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
[PMID: 26866967]
HEK293 EC50
> 10 μM
Compound: 19
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
[PMID: 24928400]
HEK-293T IC50
24.9 μM
Compound: 1, DHEA, dehydroepiandrosterone
Inhibition of G6PD in HEK293T cells assessed as accumulation of 6-phosphogluconate after 5 hrs by LC-MS/MS analysis using 6-aminonicotinamide
Inhibition of G6PD in HEK293T cells assessed as accumulation of 6-phosphogluconate after 5 hrs by LC-MS/MS analysis using 6-aminonicotinamide
[PMID: 22506561]
HeLa IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human HeLa cells after 24 hrs by MTT assay
Cytotoxic activity against human HeLa cells after 24 hrs by MTT assay
[PMID: 25456391]
HeLa IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human HeLa cells after 48 hrs by MTT assay
Cytotoxic activity against human HeLa cells after 48 hrs by MTT assay
[PMID: 25456391]
HepG2 IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human HepG2 cells after 24 hrs by MTT assay
Cytotoxic activity against human HepG2 cells after 24 hrs by MTT assay
[PMID: 25456391]
HepG2 IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human HepG2 cells after 48 hrs by MTT assay
Cytotoxic activity against human HepG2 cells after 48 hrs by MTT assay
[PMID: 25456391]
HL-60 IC50
> 20 μM
Compound: c1
Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
[PMID: 30822712]
HL-60 IC50
> 50 μM
Compound: DHEA
Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 96 hrs by MTS assay
Antiproliferative activity against human HL60 cells assessed as reduction in cell viability after 96 hrs by MTS assay
[PMID: 26866967]
HT-29 IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human HT-29 cells after 24 hrs by MTT assay
Cytotoxic activity against human HT-29 cells after 24 hrs by MTT assay
[PMID: 25456391]
HT-29 IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human HT-29 cells after 48 hrs by MTT assay
Cytotoxic activity against human HT-29 cells after 48 hrs by MTT assay
[PMID: 25456391]
HT-29 IC50
> 50 μM
Compound: DHEA
Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
[PMID: 26866967]
J774.2 CC50
198.17 μM
Compound: 1a; DHEA
Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay
Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay
[PMID: 34571489]
Jurkat IC50
46.5 μM
Compound: DHEA
Antiproliferative activity against human Jurkat cells assessed as reduction in cell viability after 96 hrs by MTS assay
Antiproliferative activity against human Jurkat cells assessed as reduction in cell viability after 96 hrs by MTS assay
[PMID: 26866967]
LNCaP IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human LNCAP cells after 24 hrs by MTT assay
Cytotoxic activity against human LNCAP cells after 24 hrs by MTT assay
[PMID: 25456391]
LNCaP IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human LNCAP cells after 48 hrs by MTT assay
Cytotoxic activity against human LNCAP cells after 48 hrs by MTT assay
[PMID: 25456391]
LNCaP IC50
> 50 μM
Compound: DHEA
Antiproliferative activity against human LNCAP cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
Antiproliferative activity against human LNCAP cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
[PMID: 26866967]
MCF7 IC50
> 20 μM
Compound: c1
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
[PMID: 30822712]
MCF7 IC50
> 200 μM
Compound: DHEA
Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
[PMID: 23880359]
MCF7 IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human MCF7 cells after 24 hrs by MTT assay
Cytotoxic activity against human MCF7 cells after 24 hrs by MTT assay
[PMID: 25456391]
MCF7 IC50
> 50 μM
Compound: DHEA
Cytotoxic activity against human MCF7 cells after 48 hrs by MTT assay
Cytotoxic activity against human MCF7 cells after 48 hrs by MTT assay
[PMID: 25456391]
MCF7 IC50
> 50 μM
Compound: DHEA
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
[PMID: 26866967]
MDA-MB-231 IC50
> 50 μM
Compound: DHEA
Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
[PMID: 26866967]
PC-12 IC50
0.29 nM
Compound: DHEA
Neuroprotective activity in rat PC12 cells assessed as inhibition of serum deprivation-induced apoptosis after 24 hrs by APO Percentage apoptosis assay
Neuroprotective activity in rat PC12 cells assessed as inhibition of serum deprivation-induced apoptosis after 24 hrs by APO Percentage apoptosis assay
[PMID: 19845386]
PC-12 IC50
1.65 nM
Compound: DHEA
Displacement of [3H]DHEA from DHEA-binding site in rat PC12 cell membranes by gamma-scintillation counting
Displacement of [3H]DHEA from DHEA-binding site in rat PC12 cell membranes by gamma-scintillation counting
[PMID: 19845386]
SMMC-7721 IC50
> 20 μM
Compound: c1
Antiproliferative activity against human SMMC7721 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human SMMC7721 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
[PMID: 30822712]
SW480 IC50
> 20 μM
Compound: c1
Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
[PMID: 30822712]
T47D IC50
2.55 μM
Compound: DHEA
Antiproliferative activity against human T47D cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
Antiproliferative activity against human T47D cells assessed as reduction in cell viability after 96 hrs by sulforhodamine B assay
[PMID: 26866967]
體外研究
(In Vitro)

DHEA (Prasterone) 是一種有效的抗凋亡因子，可逆轉(zhuǎn)前列腺癌細胞（DU145 和 LNCaP 細胞系）以及結(jié)腸癌細胞（Caco2 細胞系）中血清剝奪誘導的細胞凋亡。DHEA 可顯著降低所有 3 種癌細胞類型中血清剝奪誘導的細胞凋亡，通過 APO Percentage 測定法定量（用 DHEA 或 NGF 處理 12 小時后，細胞凋亡分別從 0.587 減少到 0.142 或 0.059），并通過流式細胞術分析（FACS）對 DU145 細胞進行定量分析。 DHEA 的抗凋亡作用與劑量有關，EC50 在納摩爾濃度下為（EC50：在 DU145 和 Caco2 細胞中分別為 11.2 nM 和 12.4 nM）[1]。DHEA 是人類的主要性類固醇前體，可直接轉(zhuǎn)化為雄激素。通過胸苷摻入試驗評估，DHEA（≥1 μM）可導致 Chub-S7 增殖的劑量依賴性抑制。DHEA 治療以劑量依賴性方式抑制分化前脂肪細胞中關鍵的糖皮質(zhì)激素調(diào)節(jié)基因 H6PDH（≥100 nM）和HSD11B1（≥1 μM）的表達。與此結(jié)果一致的是，在分化脂肪細胞中，DHEA 治療（≥1 μM）導致 11β-HSD1 氧化還原酶活性（≥1 μM）顯著降低，同時在使用最高 DHEA 劑量（25 μM DHEA）時，脫氫酶活性增加[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

雄性 B6 小鼠（五只小鼠一組）的飲食中含有 DHEA (Prasterone)（0.45% w/w），治療 8 周后，與喂食對照 AIN-76A 飲食的小鼠相比，其體溫顯著下降。類似的比較表明，對照小鼠和成對喂養(yǎng)的小鼠也存在顯著差異。在 29 次測試中，喂食 DHEA 的動物的體溫顯著低于喂食對照飲食的小鼠（26 次）；成對喂養(yǎng) DHEA 飲食的小鼠受影響較小，8/29 的數(shù)值顯著低于隨意喂食 AIN-76A 的小鼠。在 29 次測試中，喂食 DHEA 或成對喂養(yǎng) DHEA 的小鼠的體溫有 21 次顯著差異。喂食對照飲食的小鼠的體重顯著高于喂食 DHEA 或成對喂養(yǎng) DHEA ）的小鼠。每周從籠子中攝取的食物量（克/天）取平均值，第 9 周（n=3）除外。喂食 DHEA 的小鼠的食物攝入量顯著減少。根據(jù)設計，喂食 DHEA 的小鼠進食量大致相同。因此，DHEA 似乎通過限制食物和單獨的機制來降低體溫[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

288.42

Formula

C19H28O2
CAS 號
性狀

固體

顏色

White to off-white

中文名稱

脫氫表雄酮；去氫表雄酮；脫氫表雄甾酮；脫氫異雄酮；反式-脫氫異雄甾酮；反式-脫氫雄甾酮；脫氫表雄至素酮
結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : 50 mg/mL (173.36 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

Ethanol 中的溶解度 : 40 mg/mL (138.69 mM; 超聲助溶 (<60°C))

H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.4672 mL 17.3358 mL 34.6717 mL
5 mM 0.6934 mL 3.4672 mL 6.9343 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.67 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

  • 方案 二

    請依序添加每種溶劑： 10% EtOH    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.67 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 EtOH 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

  • 方案 一

    請依序添加每種溶劑： Corn Oil

    Solubility: 10 mg/mL (34.67 mM); 澄清溶液; 超聲助溶

  • 方案 二

    請依序添加每種溶劑： 15% Cremophor EL    85% Saline

    Solubility: 25 mg/mL (86.68 mM); 懸濁液; 超聲助溶

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.93%

參考文獻
Cell Assay
[2]

Chub-S7 preadipocytes and human primary preadipocytes are seeded into a 24-well plate at densities 1×105 and 2.5×105 respectively. Following overnight culture, medium is supplemented with DHEA, androstenediol, or DHEA (Prasterone) (0-100 μM). Following 24-, 48-, or 72 h incubation, cell proliferation is assessed by incubation with radiolabeled thymidine (0.2 μCi/well) for the final 6 h of culture. Proteins are precipitated with TCA, and cells are scraped in NaOH. The respective content of radiolabeled nuclear material in the resulting lysates is analyzed by scintillation counting[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[3]

Mice[3]
Mice are fed Purina Lab Chow until the start of experiments (Day 0). Groups of five mice are then fed pelleted AIN-76A diet containing either no additive or DHEA (0.45% w/w) between 0900 and 1000 hr. Diets are stored at 4°C for no longer than six months to maintain optimal activity. Mice are given the diets ad libitum, except for mice that are pair fed to mice treated with DHEA (Prasterone). The amounts of AIN-76A diet the pair-fed mice received are determined by the weight of food consumed by the DHEA-fed mice on a daily basis. Body weights (grams) are measured at different time points starting at Day 1 and ending at Day 59. Daily food intakes (grams per day) are determined by weighing the food consumed per cage of five mice. The mean±SEM values are calculated for weeks 1 to 8 (n=7); week 9 had only 3 days.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 3.4672 mL 17.3358 mL 34.6717 mL 86.6791 mL
5 mM 0.6934 mL 3.4672 mL 6.9343 mL 17.3358 mL
10 mM 0.3467 mL 1.7336 mL 3.4672 mL 8.6679 mL
15 mM 0.2311 mL 1.1557 mL 2.3114 mL 5.7786 mL
20 mM 0.1734 mL 0.8668 mL 1.7336 mL 4.3340 mL
25 mM 0.1387 mL 0.6934 mL 1.3869 mL 3.4672 mL
30 mM 0.1156 mL 0.5779 mL 1.1557 mL 2.8893 mL
40 mM 0.0867 mL 0.4334 mL 0.8668 mL 2.1670 mL
50 mM 0.0693 mL 0.3467 mL 0.6934 mL 1.7336 mL
60 mM 0.0578 mL 0.2889 mL 0.5779 mL 1.4447 mL
80 mM 0.0433 mL 0.2167 mL 0.4334 mL 1.0835 mL
100 mM 0.0347 mL 0.1734 mL 0.3467 mL 0.8668 mL
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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
DHEA
目錄號：
HY-14650
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