成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

  2. GPCR/G Protein Neuronal Signaling Immunology/Inflammation Cytoskeleton Cell Cycle/DNA Damage
  3. Guanylate Cyclase Monoamine Oxidase NO Synthase Microtubule/Tubulin
  4. Methylene Blue

Methylene Blue  (Synonyms: 亞甲藍; Basic Blue 9; CI-52015; Methylthioninium chloride)

目錄號: HY-14536 純度: 98.08%
COA 產(chǎn)品使用指南

Methylene blue (Basic Blue 9) 是一種鳥苷酸環(huán)化酶 (sGC)，單胺氧化酶 A (MAO-A) 和 NO 合酶 (NOS) 抑制劑。Methylene blue 是一種血管加壓劑，在醫(yī)療中通常用作染料。Methylene blue 通過一氧化氮合成酶/鳥苷酸環(huán)化酶信號通路降低脈沖前抑制。Methylene blue 是一種氧化還原循環(huán)化合物，能夠穿過血腦屏障。Methylene blue 是 Tau 聚集抑制劑。亞甲藍可減輕腦水腫，減弱小膠質細胞活化，減少神經(jīng)炎癥。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務

Methylene Blue Chemical Structure

Methylene Blue Chemical Structure

CAS No. : 61-73-4

1.  客戶無需承擔相應的運輸費用。

2.  同一機構（單位）同一產(chǎn)品試用裝僅限申領一次，同一機構（單位）一年內

     可免費申領三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
100 mg ￥500
In-stock
500 mg ￥850
In-stock
1 g   詢價  
5 g   詢價  

* Please select Quantity before adding items.

查看 Monoamine Oxidase 亞型特異性產(chǎn)品:

查看 NO Synthase 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Methylene blue (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3].

IC50 & Target

Guanylyl cyclase (sGC)[1].
Monoamine oxidase A (MAO-A)[1].
NO synthase (NOS)[1]
細胞效力
(Cellular Effect)
Cell Line Type Value Description References
B16-F10 IC50
5.48 μM
Compound: MB
Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay
[PMID: 34871840]
B16-F10 IC50
7.02 μM
Compound: MB
Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
[PMID: 34871840]
Fibroblast EC50
43 nM
Compound: MB
Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay
[PMID: 33214825]
HepG2 IC50
< 1 μM
Compound: Methylene blue
Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin
[PMID: 23927658]
HT-29 IC50
> 10 μM
Compound: MB
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay
[PMID: 32267685]
HT-29 IC50
9.2 μM
Compound: MB
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay
[PMID: 32267685]
Lymphocyte EC50
88 nM
Compound: MB
Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis
[PMID: 33214825]
MCF7 IC50
10.58 μM
Compound: MB
Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay
[PMID: 34871840]
MCF7 IC50
14.64 μM
Compound: MB
Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay
[PMID: 34871840]
體外研究
(In Vitro)

Methylene blue (Basic Blue 9) (4.5 μM; BV2 microglia) alters the immune profile of LPS-activated BV2 microglia and decreases the level of CD14, IL-1β, TNF-α, and CCL2 mRNA[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內研究
(In Vivo)

Methylene blue (Basic Blue 9) (50 and 100 mg/kg; i.p.; once, for 25 min; male NMRI mice) reduces absent prepulse inhibition[1].
Methylene blue (Basic Blue 9) (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2].
Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3].
Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male NMRI mice[1]
Dosage: 50 and 100 mg/kg
Administration: Intraperitoneal injection; once, for 25 minutes
Result: Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).
Animal Model: CaMKIIα-tTA transactivator mice[2]
Dosage: 20 and 40 mg/kg
Administration: Oral administration; daily, for 6 months
Result: Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.
Animal Model: TBI-treated male BALB/c mice[3]
Dosage: 2 mg/kg
Administration: Intravenous injection; once, for 1 day
Result: Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.
Animal Model: TBI-treated male BALB/c mice[3]
Dosage: 2 mg/kg
Administration: Intravenous injection; once, for 1 day
Result: Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
Clinical Trial
分子量

319.85

Formula

C16H18ClN3S
CAS 號
性狀

固體

顏色

Reddish brown to brown

中文名稱

亞甲藍
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

Store at room temperature, keep dry and cool

In solvent -80°C 2 years
-20°C 1 year
溶解性數(shù)據(jù)
細胞實驗： 

H2O 中的溶解度 : 50 mg/mL (156.32 mM; 超聲助溶)

配制儲備液
濃度 溶劑體積 質量 1 mg 5 mg 10 mg
1 mM 3.1265 mL 15.6323 mL 31.2647 mL
5 mM 0.6253 mL 3.1265 mL 6.2529 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內使用，-20°C儲存時，請在1年內使用。

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
計算結果
工作液所需濃度 : mg/mL
該產(chǎn)品水溶性佳，請具體參考實測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，如有需要，請與 MCE 中國技術支持聯(lián)系。
純度 & 產(chǎn)品資料
染色示例
參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內使用，-20°C儲存時，請在1年內使用。

可選溶劑 濃度 溶劑體積 質量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.1265 mL 15.6323 mL 31.2647 mL 78.1616 mL
5 mM 0.6253 mL 3.1265 mL 6.2529 mL 15.6323 mL
10 mM 0.3126 mL 1.5632 mL 3.1265 mL 7.8162 mL
15 mM 0.2084 mL 1.0422 mL 2.0843 mL 5.2108 mL
20 mM 0.1563 mL 0.7816 mL 1.5632 mL 3.9081 mL
25 mM 0.1251 mL 0.6253 mL 1.2506 mL 3.1265 mL
30 mM 0.1042 mL 0.5211 mL 1.0422 mL 2.6054 mL
40 mM 0.0782 mL 0.3908 mL 0.7816 mL 1.9540 mL
50 mM 0.0625 mL 0.3126 mL 0.6253 mL 1.5632 mL
60 mM 0.0521 mL 0.2605 mL 0.5211 mL 1.3027 mL
80 mM 0.0391 mL 0.1954 mL 0.3908 mL 0.9770 mL
100 mM 0.0313 mL 0.1563 mL 0.3126 mL 0.7816 mL

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的產(chǎn)品:

Your information is safe with us. * Required Fields.

   產(chǎn)品名稱：

  

* 需求量：

* 客戶姓名：

 

* Email：

* 電話：

 

* 公司或機構名稱：

   留言給我們：

Bulk Inquiry

Inquiry Information

產(chǎn)品名稱：
Methylene Blue
目錄號：
HY-14536
需求量：