Vinblastine sulfate (Synonyms: 硫酸長(zhǎng)春堿; Vincaleukoblastine sulfate salt)

目錄號(hào): HY-13780 純度: 99.86%: FAQs
Data Sheet SDS COA 產(chǎn)品使用指南

摩爾計(jì)算器

Vinblastine sulfate是一種針對(duì)各種癌癥類(lèi)型的有細(xì)胞毒性的生物堿。長(zhǎng)春花堿可抑制微管的形成，抑制nAChR的IC₅₀值為8.9 μM。

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Vinblastine sulfate Chemical Structure

CAS No. : 143-67-9

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規(guī)格	價(jià)格	是否有貨
10 mM * 1 mL in DMSO	￥765	In-stock
1 mg	￥234	In-stock
5 mg	￥515	In-stock
10 mg	￥770	In-stock
50 mg	￥2640	In-stock
100 mg	￥4200	In-stock
200 mg		詢(xún)價(jià)
500 mg		詢(xún)價(jià)

or 大包裝詢(xún)價(jià)

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Customer Review

Other Forms of Vinblastine sulfate:

Vinblastine 詢(xún)價(jià)
Vinblastine sulfate (Standard) 詢(xún)價(jià)

MCE 顧客使用本產(chǎn)品發(fā)表的 12 篇科研文獻(xiàn)

•Cancer Immunol Res. 2023 May 3;11(5):583-599. [Abstract]
•Food Chem. 2023 Jun 22, 136707.
•Int J Biol Macromol. 2022 Jun 16;S0141-8130(22)01308-3. [Abstract]
•PLoS Pathog. 2021 Aug 9;17(8):e1009838. [Abstract]
•Cell Rep Methods. 2023 Oct 23;3(10):100599. [Abstract]
•Cells. 2022, 11(19), 2942.
•Mol Pharm. 2022 Oct 21. [Abstract]
•Mol Cell Biochem. 2021 Feb;476(2):1233-1243. [Abstract]
•Biochem Pharmacol. 2020 May;175:113865. [Abstract]
•J Biol Chem. 2021, 100525.
•Mutat Res Genet Toxicol Environ Mutagen. 2016 Sep 15;808:27-37. [Abstract]
•Int J Clin Exp Pathol. 2017;10(3):3033-3042.

Vinblastine sulfate 相關(guān)產(chǎn)品

•相關(guān)化合物庫(kù):

•同靶點(diǎn)產(chǎn)品:

•同靶點(diǎn)蛋白產(chǎn)品:

生物活性
純度 & 產(chǎn)品資料
參考文獻(xiàn)

生物活性

Vinblastine sulfate is a cytotoxic alkaloid used against various cancer types. Vinblastine sulfate inhibits the formation of microtubule and suppresses nAChR with an IC₅₀ of 8.9 μM.

IC₅₀ & Target

IC50: 8.9 μM(nAChR)^[1]

細(xì)胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A-375	IC₅₀	7.2 μM Compound: Vinblastine sulfate	Cytotoxicity against human A375 cells after 48 hrs by SRB assay Cytotoxicity against human A375 cells after 48 hrs by SRB assay	[PMID: 19467877]
A549	IC₅₀	2.36 μM Compound: Vinblastine sulphate	Antiproliferative activity against human A549 cells after 48 hrs by MTS assay Antiproliferative activity against human A549 cells after 48 hrs by MTS assay	[PMID: 22546674]
A549	IC₅₀	67.3 μM Compound: Vinblastine sulfate	Cytotoxicity against human A549 cells after 48 hrs by SRB assay Cytotoxicity against human A549 cells after 48 hrs by SRB assay	[PMID: 19467877]
ACHN	IC₅₀	22.7 μM Compound: Vinblastine sulfate	Cytotoxicity against human ACHN cells after 48 hrs by SRB assay Cytotoxicity against human ACHN cells after 48 hrs by SRB assay	[PMID: 19467877]
BXPC-3	IC₅₀	1.13 μM Compound: Vinblastine sulphate	Antiproliferative activity against human BxPC3 cells after 48 hrs by MTS assay Antiproliferative activity against human BxPC3 cells after 48 hrs by MTS assay	[PMID: 22546674]
C32	IC₅₀	3 μM Compound: Vinblastine sulfate	Cytotoxicity against human C32 cells after 48 hrs by SRB assay Cytotoxicity against human C32 cells after 48 hrs by SRB assay	[PMID: 19467877]
Caco-2	IC₅₀	69 μM Compound: Vinblastine sulfate	Cytotoxicity against human Caco-2 cells after 48 hrs by SRB assay Cytotoxicity against human Caco-2 cells after 48 hrs by SRB assay	[PMID: 19467877]
COR-L23	IC₅₀	45.5 μM Compound: Vinblastine sulfate	Cytotoxicity against human COR-L23 cells after 48 hrs by SRB assay Cytotoxicity against human COR-L23 cells after 48 hrs by SRB assay	[PMID: 19467877]
DU-145	IC₅₀	4.25 μM Compound: Vinblastine sulphate	Antiproliferative activity against human DU145 cells after 48 hrs by MTS assay Antiproliferative activity against human DU145 cells after 48 hrs by MTS assay	[PMID: 22546674]
HepG2	IC₅₀	0.019 μM Compound: Vinblastine sulfate	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 23708010]
HepG2	IC₅₀	0.056 μM Compound: Vinblastine sulfate	Cytotoxicity against adriamycin-resistant human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against adriamycin-resistant human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 23708010]
HepG2	IC₅₀	0.16 μM Compound: Vinblastine sulphate	Antiproliferative activity against human HepG2 cells after 48 hrs by MTS assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTS assay	[PMID: 22546674]
HT-29	IC₅₀	0.55 μM Compound: Vinblastine	Cytotoxicity against human HT-29 cells after 48 hrs by MTS assay Cytotoxicity against human HT-29 cells after 48 hrs by MTS assay	[PMID: 21920762]
HT-29	IC₅₀	11.18 μM Compound: Vinblastine sulphate	Antiproliferative activity against human HT-29 cells after 48 hrs by MTS assay Antiproliferative activity against human HT-29 cells after 48 hrs by MTS assay	[PMID: 22546674]
K562	IC₅₀	0.001 μM Compound: Vinblastine sulphate	Antiproliferative activity against human K562 cells after 48 hrs by hemocytometer Antiproliferative activity against human K562 cells after 48 hrs by hemocytometer	[PMID: 21705223]
K562	IC₅₀	0.001 μM Compound: vinblastine sulfate	Antiproliferative activity against human K562 cells after 48 hrs by hemocytometric analysis Antiproliferative activity against human K562 cells after 48 hrs by hemocytometric analysis	[PMID: 21563750]
K562	IC₅₀	0.001 μM Compound: 1	Cell growth inhibition of human K562 cells after 48 hrs Cell growth inhibition of human K562 cells after 48 hrs	[PMID: 20546980]
K562	IC₅₀	0.001 μM Compound: 1	Antiproliferative activity against human K562 cells after 48 hrs Antiproliferative activity against human K562 cells after 48 hrs	[PMID: 20537765]
K562	IC₅₀	0.001 μM Compound: vinblastine sulfate	Antiproliferative activity against human K562 cells after 48 hrs Antiproliferative activity against human K562 cells after 48 hrs	[PMID: 19220018]
K562	IC₅₀	0.001 μM Compound: vinblastine sulfate	Antiproliferative activity against human K562 cells after 48 hrs Antiproliferative activity against human K562 cells after 48 hrs	[PMID: 17973361]
K562	IC₅₀	0.016 μM Compound: Vinblastine sulfate	Cytotoxicity against human K562 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human K562 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 23708010]
L6	ED₅₀	6.1 μM Compound: vinblastine sulphate	Cytotoxicity against human SCL6 cells by MTT assay Cytotoxicity against human SCL6 cells by MTT assay	[PMID: 12880314]
LNCaP	IC₅₀	29.3 μM Compound: Vinblastine sulfate	Cytotoxicity against human LNCAP cells after 48 hrs by SRB assay Cytotoxicity against human LNCAP cells after 48 hrs by SRB assay	[PMID: 19467877]
MCF7	IC₅₀	0.007 μM Compound: Vinblastine sulfate	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 23708010]
MCF7	IC₅₀	24.08 μM Compound: Vinblastine sulphate	Antiproliferative activity against human MCF7 cells after 48 hrs by MTS assay Antiproliferative activity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 22546674]
MDA-MB-231	IC₅₀	0.0083 μM Compound: Vinblastine sulfate	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 23708010]
MDA-MB-231	IC₅₀	31.52 μM Compound: Vinblastine sulphate	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTS assay Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTS assay	[PMID: 22546674]
NUGC-4	ED₅₀	5.3 μM Compound: vinblastine sulphate	Cytotoxicity against human NUGC4 cells by MTT assay Cytotoxicity against human NUGC4 cells by MTT assay	[PMID: 12880314]
SK-MEL-5	IC₅₀	1.74 μM Compound: Vinblastine sulphate	Antiproliferative activity against human SK-MEL-5 cells after 48 hrs by MTS assay Antiproliferative activity against human SK-MEL-5 cells after 48 hrs by MTS assay	[PMID: 22546674]
UACC-903	IC₅₀	1.65 μM Compound: Vinblastine	Cytotoxicity against human UACC903 cells after 48 hrs by MTS assay Cytotoxicity against human UACC903 cells after 48 hrs by MTS assay	[PMID: 21920762]

體外研究
(In Vitro)

Vinblastine does not depolymerize spindle microtubules, yet it powerfully blocks mitosis (for example, IC₅₀ 0.8 nM in HeLa cells) and cells die by apoptosis^[2]. In NB4 cells, vinblastine produces alteration of p53 and DNA fragmentation. Vinblastine treatment has an antiproliferative effect via the induction of apoptosis producing Bax/Bcl-2 imbalance. Vinblastine treatment suppresses NFκB expression and depresses NFκB-DNA binding activity while maintaining JNK activation that subsequently results in apoptotic response through caspase-dependent pathway^[3]. Vinblastine is found to trigger apoptosis as evidenced by the loss of mitochondrial membrane potential, the release of both cytochrome c and apoptosis inducing factor, activation of caspase-9 and 3, and cleavage of Poly (ADP-ribose)-Polymerase^[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

Vinblastine is a widely used anticancer drug with undesired side effects. Its conjugation with carrier molecules could be an efficient strategy to reduce these side effects^[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

909.05

Formula

C₄₆H₆₀N₄O₁₃S

CAS 號(hào)

143-67-9

性狀

固體

顏色

White to off-white

中文名稱(chēng)

硫酸長(zhǎng)春堿

結(jié)構(gòu)分類(lèi)

初始來(lái)源

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性數(shù)據(jù)

細(xì)胞實(shí)驗(yàn)：

H₂O 中的溶解度 : 50 mg/mL (55.00 mM; 超聲助溶)

DMSO 中的溶解度 : ≥ 44 mg/mL (48.40 mM; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲(chǔ)備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	1.1000 mL	5.5002 mL	11.0005 mL
5 mM	0.2200 mL	1.1000 mL	2.2001 mL
10 mM	0.1100 mL	0.5500 mL	1.1000 mL

查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

* 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

摩爾計(jì)算器
稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動(dòng)物實(shí)驗(yàn)：

請(qǐng)根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥方式選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

方案一

請(qǐng)依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.75 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請(qǐng)依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (2.75 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液中，混合均勻。
2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
方案三

請(qǐng)依序添加每種溶劑： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.75 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實(shí)驗(yàn)周期在半個(gè)月以上的動(dòng)物實(shí)驗(yàn)酌情使用。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL玉米油中，混合均勻。

以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶。

方案一

請(qǐng)依序添加每種溶劑： PBS
Solubility: 50 mg/mL (55.00 mM); 澄清溶液; 超聲助溶

動(dòng)物溶解方案計(jì)算器

請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只

由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量

計(jì)算結(jié)果

工作液所需濃度 : mg/mL

該產(chǎn)品水溶性佳，請(qǐng)具體參考實(shí)測(cè) 水 / PBS / Saline 中的溶解度數(shù)據(jù)。

您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。

純度 & 產(chǎn)品資料

純度: 99.86%

選擇批次：

Data Sheet (634 KB) SDS (418 KB)

COA (298 KB) LCMS (94 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻(xiàn)

[1]. McKay DB, et al. Nicotinic and nonnicotinic receptor-mediated actions of vinblastine. Proc Soc Exp Biol Med. 1993 Jul;203(3):372-6. [Content Brief]

[2]. Pandya P, et al. Molecular recognition pattern of cytotoxic alkaloid vinblastine with multiple targets. J Mol Graph Model. 2014 Nov;54:1-9. [Content Brief]

[3]. Calvi?o E, et al. JNK and NFκB dependence of apoptosis induced by vinblastine in human acute promyelocytic leukaemia cells. Cell Biochem Funct. 2015 Jun;33(4):211-9. [Content Brief]

[4]. Selimovic D, et al. Vinblastine-induced apoptosis of melanoma cells is mediated by Ras homologous A protein (Rho A) via mitochondrial and non-mitochondrial-dependent mechanisms. Apoptosis. 2013 Aug;18(8):980-97. [Content Brief]

[5]. Bánóczi Z, et al. Synthesis and in vitro antitumor effect of vinblastine derivative-oligoarginine conjugates. Bioconjug Chem. 2010 Nov 17;21(11):1948-55. [Content Brief]

Vinblastine sulfate 相關(guān)分類(lèi)

完整儲(chǔ)備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.1000 mL	5.5002 mL	11.0005 mL	27.5012 mL
	5 mM	0.2200 mL	1.1000 mL	2.2001 mL	5.5002 mL
	10 mM	0.1100 mL	0.5500 mL	1.1000 mL	2.7501 mL
	15 mM	0.0733 mL	0.3667 mL	0.7334 mL	1.8334 mL
	20 mM	0.0550 mL	0.2750 mL	0.5500 mL	1.3751 mL
	25 mM	0.0440 mL	0.2200 mL	0.4400 mL	1.1000 mL
	30 mM	0.0367 mL	0.1833 mL	0.3667 mL	0.9167 mL
	40 mM	0.0275 mL	0.1375 mL	0.2750 mL	0.6875 mL
H₂O	50 mM	0.0220 mL	0.1100 mL	0.2200 mL	0.5500 mL

* 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Vinblastine143-67-9Vincaleukoblastine硫酸長(zhǎng)春堿Microtubule/TubulinAutophagyInhibitorinhibitorinhibit

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Vinblastine sulfate (Synonyms: 硫酸長(zhǎng)春堿; Vincaleukoblastine sulfate salt)

Customer Review

Other Forms of Vinblastine sulfate:

MCE 顧客使用本產(chǎn)品發(fā)表的 12 篇科研文獻(xiàn)

Vinblastine sulfate 相關(guān)產(chǎn)品

•相關(guān)化合物庫(kù):

•同靶點(diǎn)產(chǎn)品:

•同靶點(diǎn)蛋白產(chǎn)品:

生物活性

純度 & 產(chǎn)品資料

參考文獻(xiàn)

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Vinblastine sulfate (Synonyms: 硫酸長(zhǎng)春堿; Vincaleukoblastine sulfate salt)

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Vinblastine sulfate 相關(guān)抗體

MCE 顧客使用本產(chǎn)品發(fā)表的 12 篇科研文獻(xiàn)

Vinblastine sulfate 相關(guān)產(chǎn)品

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純度 & 產(chǎn)品資料

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Vinblastine sulfate 相關(guān)抗體:

摩爾計(jì)算器

稀釋計(jì)算器

Vinblastine sulfate 相關(guān)分類(lèi)

完整儲(chǔ)備液配制表

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