Mitoxantrone (Synonyms: 米托蒽醌; Mitozantrone; NSC 301739)

目錄號: HY-13502 純度: 98.70%: FAQs
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摩爾計算器

Mitoxantrone 是一種有效的拓撲異構(gòu)酶II (topoisomerase II) 抑制劑。Mitoxantrone 也可抑制蛋白激酶 C (PKC)，其 IC₅₀ 值為 8.5 μM。Mitoxantrone 誘導 B- 慢性淋巴細胞白血病 (B-CLL) 細胞凋亡 (apoptosis)。Mitoxantrone 具有抗腫瘤活性。Mitoxantrone 還具有抗正痘病毒 (orthopoxvirus) 活性，對牛痘和猴痘的 EC₅₀ 分別為 0.25 μM 和 0.8 μM。

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Mitoxantrone Chemical Structure

CAS No. : 65271-80-9

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規(guī)格	價格	是否有貨
10 mM * 1 mL in DMSO	￥408	In-stock
25 mg	￥371	In-stock
50 mg	￥515	In-stock
100 mg	￥744	In-stock
200 mg		詢價
500 mg		詢價

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Other Forms of Mitoxantrone:

MCE 顧客使用本產(chǎn)品發(fā)表的 26 篇科研文獻

•Adv Mater. 2024 Apr 21:e2313097. [Abstract]
•Nat Cell Biol. 2024 May 7. [Abstract]
•EMBO Mol Med. 2024 Oct 11. [Abstract]
•Mol Cell. 2024 Jun 18:S1097-2765(24)00447-7. [Abstract]
•Cell Rep Med. 2024 Feb 20;5(2):101388. [Abstract]
•J Am Chem Soc. 2022 Jun 15;144(23):10407-10416. [Abstract]
•Nat Commun. 2020 Apr 14;11(1):1792. [Abstract]
•Acta Pharmacol Sin. 2024 Aug 15. [Abstract]
•Pharmacol Res. 2024 May 9:204:107208. [Abstract]
•Acta Pharmacol Sin. 2022 Aug 31. [Abstract]
•Int J Biol Sci. 2022 Mar 21;18(6):2568-2582. [Abstract]
•Talanta. 2024 Mar 23, 125987.

•Cell Oncol. 2020 Dec;43(6):1099-1116. [Abstract]
•Cell Rep Methods. 2023 Oct 23;3(10):100599. [Abstract]
•Drug Deliv. 2022 Dec;29(1):2561-2578. [Abstract]
•Cancers. 2020 Aug 4;12(8):2169. [Abstract]
•J Mol Med (Berl). 2019 Aug;97(8):1183-1193. [Abstract]
•J Pharm Pharmacol. 2022 Jan 5;74(1):41-56. [Abstract]
•J Pharm Pharmacol. 23 November 2021.
•BMC Cancer. 2021 Oct 13;21(1):1101. [Abstract]
•Pharm Dev Technol. 2021 Apr 30;1-9. [Abstract]
•Med Oncol. 2020 Nov 19;37(12):116. [Abstract]
•Exp Cell Res. 2020 Aug 1;393(1):112054. [Abstract]
•bioRxiv. 2024 September 07.
•bioRxiv. 2023 Jan 13.
•Research Square Print. November 28th, 2022.

Mitoxantrone 相關(guān)產(chǎn)品

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查看 Topoisomerase 亞型特異性產(chǎn)品:

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Topoisomerase Topo I Topo II

查看 PKC 亞型特異性產(chǎn)品:

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PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKCι

生物活性
實驗參考方法
純度 & 產(chǎn)品資料
參考文獻

生物活性

Mitoxantrone is a potent topoisomerase II inhibitor. Mitoxantrone also inhibits protein kinase C (PKC) activity with an IC₅₀ of 8.5 μM. Mitoxantrone induces apoptosis of B-CLL (B-chronic lymphocytic leukaemia) cells. Mitoxantrone shows antitumor activity^[1]^[2]^[3]^[4]. Mitoxantrone also has anti-orthopoxvirus activity with EC₅₀s of 0.25 μM and and 0.8 μM for cowpox and monkeypox, respectively^[5].

IC₅₀ & Target^[1]^[2]

PKC

8.5 μM (IC₅₀)

Topoisomerase II

細胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
2008	IC₅₀	7.8 nM Compound: Mitoxantrone	Antiproliferative activity against human 2008 cells assessed as reduction in cell viability after 5 days by MTS/PMS assay Antiproliferative activity against human 2008 cells assessed as reduction in cell viability after 5 days by MTS/PMS assay	[PMID: 31465686]
A2780	IC₅₀	0.00055 μM Compound: Mitoxantrone	Concentration required to inhibit A2780-cell growth by 50% Concentration required to inhibit A2780-cell growth by 50%	[PMID: 9703471]
A2780	IC₅₀	0.00055 μM Compound: mitoxantrone	Cytotoxic potency required to inhibit A2780 cell growth by 50% after cell drug contact for 96 hrs Cytotoxic potency required to inhibit A2780 cell growth by 50% after cell drug contact for 96 hrs	[PMID: 9371240]
A-375	IC₅₀	111.5 nM Compound: Mitoxantrone	Cytotoxicity against human A375 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and measured after 48 hrs by CCK8 assay Cytotoxicity against human A375 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and measured after 48 hrs by CCK8 assay	[PMID: 30925340]
A549	IC₅₀	0.036 nM Compound: Mitoxantrone	Antiproliferative activity against human A549 cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human A549 cells after 72 hrs by sulforhodamine B assay	[PMID: 30485090]
A549	IC₅₀	15.7 μg/mL Compound: Mitoxantrone	Cytotoxicity against human A549 cells after 24 hrs by MTT assay Cytotoxicity against human A549 cells after 24 hrs by MTT assay	[PMID: 26599534]
A549	IC₅₀	25.3 μg/mL Compound: Mito	Cytotoxicity against Homo sapiens (human) A549 cells after 24 hr by MTT assay Cytotoxicity against Homo sapiens (human) A549 cells after 24 hr by MTT assay	10.1007/s00044-012-0325-2
A549	IC₅₀	3 nM Compound: mitoxantrone	Tested for inhibitory activity against human tumor cell line A549 (a non small, drug resistant cell line that does not produce P-glycoprotein) of lung carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line A549 (a non small, drug resistant cell line that does not produce P-glycoprotein) of lung carcinoma using sulforhodamine B assay.	[PMID: 10956214]
A549	IC₅₀	7.25 μM Compound: Table S1, R142C2	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 30684866]
A549	IC₅₀	7.8 μM Compound: Mitoxantrone	Cytotoxicity against human A549 cells after 24 hrs by MTT assay Cytotoxicity against human A549 cells after 24 hrs by MTT assay	[PMID: 21507644]
A549	IC₅₀	7.8 μM Compound: Mitoxantrone	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 21458279]
A549	IC₅₀	7.83 μM Compound: MX	Cytotoxicity against human A549 cells after 72 hrs Cytotoxicity against human A549 cells after 72 hrs	[PMID: 21354791]
A549	IC₅₀	8 μM Compound: Mitoxantrone	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 19615900]
A549	GI₅₀	8.3 μM Compound: Mitoxantrone	Growth inhibition of human A549 cells by MTT assay Growth inhibition of human A549 cells by MTT assay	[PMID: 20153184]
Bel-7402	IC₅₀	116.6 nM Compound: Mitoxantrone	Cytotoxicity against human Bel7402 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and measured after 48 hrs by CCK8 assay Cytotoxicity against human Bel7402 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and measured after 48 hrs by CCK8 assay	[PMID: 30925340]
C6	IC₅₀	10.9 μg/mL Compound: Mito	Cytotoxicity against Rattus norvegicus (rat) C6 cells after 24 hr by MTT assay Cytotoxicity against Rattus norvegicus (rat) C6 cells after 24 hr by MTT assay	10.1007/s00044-012-0325-2
C6	IC₅₀	11 μg/mL Compound: Mitoxantrone	Cytotoxicity against rat C6 cells after 24 hrs by MTT assay Cytotoxicity against rat C6 cells after 24 hrs by MTT assay	[PMID: 26599534]
Caco-2	IC₅₀	> 10 μM Compound: 1	Cytotoxicity against NAE-knockdown human Caco2 cells after 72 hrs by MTT assay Cytotoxicity against NAE-knockdown human Caco2 cells after 72 hrs by MTT assay	[PMID: 29232579]
Caco-2	IC₅₀	1.4 μM Compound: 1	Cytotoxicity against human Caco2 cells after 72 hrs by MTT assay Cytotoxicity against human Caco2 cells after 72 hrs by MTT assay	[PMID: 29232579]
Cancer cell lines	IC₅₀	0.75 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human H460M cancer cell line was determined after 144 hr Cytotoxicity against human H460M cancer cell line was determined after 144 hr	[PMID: 15456268]
Cancer cell lines	IC₅₀	0.85 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human H460M cancer cell line was determined after 1 hr Cytotoxicity against human H460M cancer cell line was determined after 1 hr	[PMID: 15456268]
Cancer cell lines	IC₅₀	6.8 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human MKN45 cancer cell line was determined after 1 hr Cytotoxicity against human MKN45 cancer cell line was determined after 1 hr	[PMID: 15456268]
Cancer cell lines	IC₅₀	75 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human PC3 cancer cell line was determined after 1 hr Cytotoxicity against human PC3 cancer cell line was determined after 1 hr	[PMID: 15456268]
CCD 19Lu	IC₅₀	> 160 nM Compound: Mitoxantrone	Cytotoxicity in human CCD-19Lu cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in human CCD-19Lu cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 30108778]
CCRF-CEM	IC₅₀	0.036 μM Compound: mitoxantrone	Cytotoxicity against human CCRF-CEM cells assessed as cell viability after 48 hrs by celltiter-blue assay Cytotoxicity against human CCRF-CEM cells assessed as cell viability after 48 hrs by celltiter-blue assay	[PMID: 22582991]
CH1	IC₅₀	0.00265 μM Compound: Mitoxantrone	Concentration required to inhibit CH1-cell growth by 50% Concentration required to inhibit CH1-cell growth by 50%	[PMID: 9703471]
CH1	IC₅₀	0.00265 μM Compound: mitoxantrone	Cytotoxic potency required to inhibit CH1 cell growth by 50% Cytotoxic potency required to inhibit CH1 cell growth by 50%	[PMID: 9371240]
CHO	IC₅₀	22.5 μM Compound: mitoxantrone	Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits Inhibition of Cav1.2 current measured using QPatch automatic path clamp system in CHO cells expressing Cav1.2, beta-2 and alpha-2/delta-1 subunits	[PMID: 23812503]
Daudi	IC₅₀	0.005 μM Compound: Mit	Antiproliferative activity against human Daudi cells after 72 hrs by MTT assay Antiproliferative activity against human Daudi cells after 72 hrs by MTT assay	[PMID: 25998504]
G-361	IC₅₀	0.00065 μM Compound: mitoxantrone	Cytotoxic potency required to inhibit G-361 cell growth by 50% Cytotoxic potency required to inhibit G-361 cell growth by 50%	[PMID: 9371240]
H22	IC₅₀	187 nM Compound: Mitoxantrone	Dark toxicity against mouse H22 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and measured after 48 hrs by CCK8 assay Dark toxicity against mouse H22 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and measured after 48 hrs by CCK8 assay	[PMID: 30925340]
HaCaT	IC₅₀	3.9 μM Compound: Mitoxantrone	Cytotoxicity against human HaCaT cells by Cell titre-blue assay Cytotoxicity against human HaCaT cells by Cell titre-blue assay	[PMID: 26684177]
HCC78	IC₅₀	13.83 nM Compound: Mitoxantrone	Cytotoxicity in human HCC78 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in human HCC78 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 30108778]
HCT-116	IC₅₀	0.022 μM Compound: Mx	Cytotoxicity against human HCT116 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay	[PMID: 21444205]
HCT-116	IC₅₀	0.025 μM Compound: Mit	Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 25998504]
HCT-116	IC₅₀	1.61 μM Compound: Mitoxantrone	Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 29407981]
HCT-116	IC₅₀	110 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human HCT116 cancer cell line was determined after 1 hr Cytotoxicity against human HCT116 cancer cell line was determined after 1 hr	[PMID: 15456268]
HCT-116	IC₅₀	3.96 μM Compound: Mitoxantrone	Cytotoxicity against human HCT116 cells by MTT assay Cytotoxicity against human HCT116 cells by MTT assay	[PMID: 20951582]
HCT-116	IC₅₀	5.8 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human HCT116 cancer cell line was determined after 144 hr Cytotoxicity against human HCT116 cancer cell line was determined after 144 hr	[PMID: 15456268]
HCT-116	IC₅₀	7.2 μM Compound: Mitoxantrone	Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay	[PMID: 21507644]
HCT-116	IC₅₀	7.2 μM Compound: Mitoxantrone	Cytotoxicity against human HCT116 cells by MTT assay Cytotoxicity against human HCT116 cells by MTT assay	[PMID: 21458279]
HEK293	IC₅₀	0.4 μM Compound: mitoxantrone	Inhibition of human MATE1-mediated [14]-metformin uptake expressed in HEK293 cells after 1.5 mins by scintillation counting analysis Inhibition of human MATE1-mediated [14]-metformin uptake expressed in HEK293 cells after 1.5 mins by scintillation counting analysis	[PMID: 23241029]
HEK293	IC₅₀	0.53 μM Compound: mitoxantrone	Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells up to 500 uM after 1.5 mins by fluorescence assay Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells up to 500 uM after 1.5 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	0.53 μM Compound: mitoxantrone	Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE1-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	0.83 μM Compound: mitoxantrone	Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells after 1.5 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	10 nM Compound: Mitoxantrone	Cytotoxicity against HEK293 cells after 72 hrs by MTT assay Cytotoxicity against HEK293 cells after 72 hrs by MTT assay	[PMID: 24611893]
HEK293	IC₅₀	11.7 nM Compound: Mitoxantrone	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 5 days by MTS/PMS assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 5 days by MTS/PMS assay	[PMID: 31465686]
HEK293	IC₅₀	26.6 nM Compound: Mitoxantrone	Cytotoxicity against HEK293 cells overexpressing ABCG2 after 72 hrs by MTT assay Cytotoxicity against HEK293 cells overexpressing ABCG2 after 72 hrs by MTT assay	[PMID: 24611893]
HEK293	IC₅₀	43.9 μM Compound: mitoxantrone	Inhibition of human OCT1-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay Inhibition of human OCT1-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	60.5 μM Compound: mitoxantrone	Inhibition of human OCT3-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay Inhibition of human OCT3-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay	[PMID: 23241029]
HEK293	IC₅₀	73.3 μM Compound: mitoxantrone	Inhibition of human OCT2-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay Inhibition of human OCT2-mediated ASP+ uptake expressed in HEK293 cells after 3 mins by fluorescence assay	[PMID: 23241029]
HEK-293T	IC₅₀	< 0.1 μM Compound: mitoxantrone	Cytotoxicity against human 293T cells expressing telomerase after 96 hrs by MTT assay Cytotoxicity against human 293T cells expressing telomerase after 96 hrs by MTT assay	[PMID: 18754611]
HeLa	IC₅₀	< 0.1 μM Compound: mitoxantrone	Cytotoxicity against human HeLa cells expressing telomerase after 96 hrs by MTT assay Cytotoxicity against human HeLa cells expressing telomerase after 96 hrs by MTT assay	[PMID: 18754611]
HeLa	IC₅₀	0.044 μM Compound: Mit	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 25998504]
HeLa	EC₅₀	0.55 μg/mL Compound: 4	Effective concentration required to inhibit by 50% the growth of HeLa S3 cells Effective concentration required to inhibit by 50% the growth of HeLa S3 cells	[PMID: 3172129]
HeLa	IC₅₀	2.35 μM Compound: Mitoxantrone	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 29407981]
HepG2	IC₅₀	11.05 μM Compound: Mitoxantrone	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 24794747]
HepG2	IC₅₀	15.33 μM Compound: MTZ	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 33191085]
HepG2	IC₅₀	18.6 μM Compound: Mitoxantrone	Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 29407981]
HL-60	IC₅₀	0.0025 μM Compound: Mitoxantrone	Antiproliferative activity against human HL60 cells after 72 hrs by SRB assay Antiproliferative activity against human HL60 cells after 72 hrs by SRB assay	[PMID: 22944121]
HL-60	GI₅₀	0.01 μM Compound: mitoxantrone	Growth inhibition of human HL60 cells by Almar blue assay Growth inhibition of human HL60 cells by Almar blue assay	[PMID: 17418582]
HL-60	IC₅₀	0.016 μM Compound: mitoxantrone	Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by celltiter-blue assay Cytotoxicity against human HL60 cells assessed as cell viability after 48 hrs by celltiter-blue assay	[PMID: 22582991]
HL-60	IC₅₀	0.024 nM Compound: Mitoxantrone	Antiproliferative activity against human HL60 cells after 72 hrs by CCK8 assay Antiproliferative activity against human HL60 cells after 72 hrs by CCK8 assay	[PMID: 30485090]
HL-60	IC₅₀	0.063 μM Compound: MX	Cytotoxicity against human HL60 cells after 72 hrs Cytotoxicity against human HL60 cells after 72 hrs	[PMID: 20961767]
HL-60	IC₅₀	0.063 μM Compound: MX, mitoxantrone	Cytotoxicity against human mitoxantrone-sensitive HL60 cells after 72 hrs Cytotoxicity against human mitoxantrone-sensitive HL60 cells after 72 hrs	[PMID: 18507368]
HL-60	GI₅₀	0.192 μg/mL Compound: mitoxantrone	Cytotoxicity against human HL60 cells after 48 hrs by cell titer-blue assay Cytotoxicity against human HL60 cells after 48 hrs by cell titer-blue assay	[PMID: 19743858]
HL-60	IC₅₀	0.29 μg/mL Compound: mitoxantrone	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 10843567]
HL-60	GI₅₀	0.33 nM Compound: MX, mitoxantrone	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 17962028]
HL-60	IC₅₀	1.51 μM Compound: MX	Cytotoxicity against human HL60/MX2 cells after 72 hrs Cytotoxicity against human HL60/MX2 cells after 72 hrs	[PMID: 20961767]
HL-60	IC₅₀	4 nM Compound: MITO	Antiproliferative activity against human HL60 cells after 72 hrs by SRB assay Antiproliferative activity against human HL60 cells after 72 hrs by SRB assay	[PMID: 29945793]
HL-60	IC₅₀	4 nM Compound: MITO	Cytotoxicity against human HL60 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human HL60 cells after 72 hrs by sulforhodamine B assay	[PMID: 28342398]
HL-60	IC₅₀	6.3 μM Compound: Mitoxantrone	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 19615900]
HL-60	IC₅₀	6.8 μM Compound: Mitoxantrone	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 19926279]
HL-60	IC₅₀	7.5 μM Compound: Mitoxantrone	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 20951582]
HL-60	GI₅₀	7.8 μM Compound: Mitoxantrone	Growth inhibition of human HL60 cells by MTT assay Growth inhibition of human HL60 cells by MTT assay	[PMID: 20153184]
HL-60	IC₅₀	7.9 μM Compound: Mitoxantrone	Cytotoxicity against human HL60 cells by MTT assay Cytotoxicity against human HL60 cells by MTT assay	[PMID: 21458279]
HL-60	IC₅₀	8.1 μM Compound: MX	Cytotoxicity against human HL60 cells after 72 hrs Cytotoxicity against human HL60 cells after 72 hrs	[PMID: 21354791]
HL-60	IC₅₀	8.2 μM Compound: Mitoxantrone	Cytotoxicity against human HL60 cells after 24 hrs by MTT assay Cytotoxicity against human HL60 cells after 24 hrs by MTT assay	[PMID: 21507644]
HL60/MX2	IC₅₀	0.561 μM Compound: Mitoxantrone	Antiproliferative activity against human HL60/MX2 cells after 72 hrs by SRB assay Antiproliferative activity against human HL60/MX2 cells after 72 hrs by SRB assay	[PMID: 22944121]
HL60/MX2	IC₅₀	1.51 μM Compound: MX, mitoxantrone	Cytotoxicity against mitoxantrone-resistant human HL60/MX2 cells after 72 hrs Cytotoxicity against mitoxantrone-resistant human HL60/MX2 cells after 72 hrs	[PMID: 18507368]
HL60/MX2	IC₅₀	148 nM Compound: MITO	Antiproliferative activity against human HL60/MX2 cells after 72 hrs by SRB assay Antiproliferative activity against human HL60/MX2 cells after 72 hrs by SRB assay	[PMID: 29945793]
HL60/MX2	IC₅₀	148 nM Compound: MITO	Cytotoxicity against human HL60/MX2 cells after 72 hrs by sulforhodamine B assay Cytotoxicity against human HL60/MX2 cells after 72 hrs by sulforhodamine B assay	[PMID: 28342398]
HL60/MX2	GI₅₀	26 nM Compound: MX, mitoxantrone	Cytotoxicity against DNA topoisomerase-2 deficient human HL60/MX2 cells by MTT assay Cytotoxicity against DNA topoisomerase-2 deficient human HL60/MX2 cells by MTT assay	[PMID: 17962028]
HL60/MX2	GI₅₀	4.42 μg/mL Compound: mitoxantrone	Cytotoxicity against human HL60/MX2 cells after 48 hrs by cell titer-blue assay Cytotoxicity against human HL60/MX2 cells after 48 hrs by cell titer-blue assay	[PMID: 19743858]
HT-29	IC₅₀	0.01 μM Compound: mitoxantrone	Cytotoxic potency required to inhibit HT-29 cell growth by 50% after cell drug contact for 144 hrs Cytotoxic potency required to inhibit HT-29 cell growth by 50% after cell drug contact for 144 hrs	[PMID: 9371240]
HT-29	IC₅₀	0.01 μM Compound: MX	Concentration required to inhibit cell growth by 50% in vitro against Human colon adenocarcinoma cell line Concentration required to inhibit cell growth by 50% in vitro against Human colon adenocarcinoma cell line	[PMID: 11563932]
HT-29	IC₅₀	0.025 μM Compound: Mx	Antiproliferative activity against HT29 cells by MTT assay after 72 hrs Antiproliferative activity against HT29 cells by MTT assay after 72 hrs	[PMID: 16824751]
HT-29	IC₅₀	21.65 μM Compound: Mitoxantrone	Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 29407981]
HT-29	IC₅₀	8.7 μM Compound: Mitoxantrone	Cytotoxicity against human HT-29 cells by MTT assay Cytotoxicity against human HT-29 cells by MTT assay	[PMID: 19615900]
HT-29	GI₅₀	8.9 μM Compound: Mitoxantrone	Growth inhibition of human HT-29 cells by MTT assay Growth inhibition of human HT-29 cells by MTT assay	[PMID: 20153184]
Ishikawa	IC₅₀	0.01 μM Compound: Mit	Antiproliferative activity against human Ishikawa cells after 72 hrs by MTT assay Antiproliferative activity against human Ishikawa cells after 72 hrs by MTT assay	[PMID: 25998504]
K562	IC₅₀	0.0026 μM Compound: mitoxantrone	Cytotoxicity against human K562 cells after 5 days by XTT assay Cytotoxicity against human K562 cells after 5 days by XTT assay	[PMID: 18076140]
K562	IC₅₀	0.0334 μg/mL Compound: mitoxantrone	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 10843567]
K562	IC₅₀	0.42 μM Compound: Mitoxantrone	Growth inhibition of human K562 cells after 72 hrs by MTS method Growth inhibition of human K562 cells after 72 hrs by MTS method	[PMID: 18258442]
K562	IC₅₀	0.51 μM Compound: mitoxantrone	Cytotoxicity against human K562 cells assessed as cell viability after 48 hrs by celltiter-blue assay Cytotoxicity against human K562 cells assessed as cell viability after 48 hrs by celltiter-blue assay	[PMID: 22582991]
K562	IC₅₀	2 μg/mL Compound: mitoxanthrone	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 12027731]
K562	IC₅₀	2.35 μM Compound: MTZ	Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 33191085]
K562	IC₅₀	4.55 μM Compound: Mitoxantrone	Antiproliferative activity against human K562 cells after 48 hrs by MTT assay Antiproliferative activity against human K562 cells after 48 hrs by MTT assay	[PMID: 29407981]
KB	EC₅₀	0.355 μg/mL Compound: Mitoxantrone	Antiproliferative activity against human KB/HeLa cells after 48 hrs by XTT assay Antiproliferative activity against human KB/HeLa cells after 48 hrs by XTT assay	[PMID: 21458262]
KB	EC₅₀	0.36 μM Compound: mitoxantrone	Antiproliferative activity against human KB/HeLa cells after 48 hrs Antiproliferative activity against human KB/HeLa cells after 48 hrs	[PMID: 23395656]
KB	EC₅₀	0.42 μM Compound: Mitoxantrone	Cytotoxicity against human KB/HeLa cells after 48 hrs by resazurin dye assay Cytotoxicity against human KB/HeLa cells after 48 hrs by resazurin dye assay	[PMID: 23988351]
L1210	EC₅₀	> 5 μg/mL Compound: 4	Effective concentration required to inhibit by 50% the growth of L1210 cells Effective concentration required to inhibit by 50% the growth of L1210 cells	[PMID: 3172129]
L1210	IC₅₀	0.00004 μM Compound: mitoxantrone	Cytotoxic potency required to inhibit L1210 cell growth by 50% after cell drug contact for 48 hrs Cytotoxic potency required to inhibit L1210 cell growth by 50% after cell drug contact for 48 hrs	[PMID: 9371240]
L1210	IC₅₀	10 nM Compound: mitoxantrone	Tested for inhibitory activity against murine cell line L12110, a strain sensitive to doxorubicin using MTT assay. Tested for inhibitory activity against murine cell line L12110, a strain sensitive to doxorubicin using MTT assay.	[PMID: 10956214]
L1210	IC₅₀	15 μM Compound: Mitoxantrone	Cytotoxicity against mouse L1210 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against mouse L1210 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 28222315]
L1210	IC₅₀	39 nM Compound: Mitoxantrone	Antitumor activity against murine L1210 resistant cell line by using MTT assay Antitumor activity against murine L1210 resistant cell line by using MTT assay	[PMID: 7699715]
L1210	IC₅₀	39 nM Compound: mitoxantrone	Tested for inhibitory activity against murine cell line L12110, a strain resistant to doxorubicin using MTT assay. Tested for inhibitory activity against murine cell line L12110, a strain resistant to doxorubicin using MTT assay.	[PMID: 10956214]
L1210	IC₅₀	5 nM Compound: Mitoxantrone	Anti-tumor activity against murine L1210 sensitive cell line by using MTT assay Anti-tumor activity against murine L1210 sensitive cell line by using MTT assay	[PMID: 7699715]
LoVo	IC₅₀	12 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human LoVo cancer cell line was determined after 1 hr Cytotoxicity against human LoVo cancer cell line was determined after 1 hr	[PMID: 15456268]
LoVo	IC₅₀	3.3 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human LoVo cancer cell line was determined after 144 hr Cytotoxicity against human LoVo cancer cell line was determined after 144 hr	[PMID: 15456268]
MCF7	GI₅₀	3.93 μM Compound: MX, mitoxantrone	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay	[PMID: 17962028]
MCF7	IC₅₀	380.2 nM Compound: Mitoxantrone	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and measured after 48 hrs by CCK8 assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability preincubated for 5 hrs followed by compound washout and measured after 48 hrs by CCK8 assay	[PMID: 30925340]
MCF7	IC₅₀	41 nM Compound: mitoxantrone	Tested for inhibitory activity against human tumor cell line MCF7, a strain resistant to mitoxantrone of breast carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line MCF7, a strain resistant to mitoxantrone of breast carcinoma using sulforhodamine B assay.	[PMID: 10956214]
MCF7	IC₅₀	7.1 μM Compound: Mitoxantrone	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 21458279]
MCF7	IC₅₀	7.1 μM Compound: Mitoxantrone	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 19615900]
MCF7	IC₅₀	72 nM Compound: mitoxantrone	Tested for inhibitory activity against human tumor cell line D40, a strain resistant to doxorubicin of breast carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line D40, a strain resistant to doxorubicin of breast carcinoma using sulforhodamine B assay.	[PMID: 10956214]
MCF7	IC₅₀	9 nM Compound: mitoxantrone	Tested for inhibitory activity against human tumor cell line MCF7, a strain sensitive to doxorubicin of breast carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line MCF7, a strain sensitive to doxorubicin of breast carcinoma using sulforhodamine B assay.	[PMID: 10956214]
MDA-MB-231	IC₅₀	0.96 μM Compound: mitoxantrone	Antiproliferative activity against human MDA-MB-231 cells by WST-1 method after 72 hrs Antiproliferative activity against human MDA-MB-231 cells by WST-1 method after 72 hrs	[PMID: 17335189]
MDA-MB-435	IC₅₀	0.35 nM Compound: Mitoxantrone	Antiproliferative activity against MDA435/LCC6 cells by ELISA Antiproliferative activity against MDA435/LCC6 cells by ELISA	[PMID: 17154505]
MDA-MB-435	IC₅₀	1442 nM Compound: Mitoxantrone	Antiproliferative activity against multidrug resistant MDA435/LCC6 cells by ELISA Antiproliferative activity against multidrug resistant MDA435/LCC6 cells by ELISA	[PMID: 17154505]
MDA-MB-435	IC₅₀	646 nM Compound: Mitoxantrone	Antiproliferative activity against multidrug resistant MDA435/LCC6 cells in presence of 5 uM 1,13-bis[4'-((5,7-dihydroxy)-4H-chromen-4-on-2-yl)phenyl]-1,4,7,10,13-pentaoxatridecane by ELISA Antiproliferative activity against multidrug resistant MDA435/LCC6 cells in presence of 5 uM 1,13-bis[4'-((5,7-dihydroxy)-4H-chromen-4-on-2-yl)phenyl]-1,4,7,10,13-pentaoxatridecane by ELISA	[PMID: 17154505]
MDCK-II	IC₅₀	0.19 μM Compound: mitoxantrone	Inhibition of human MATE1-mediated [14]-metformin uptake expressed in polarized MDCK2 cells after 5 mins by liquid scintillation counting analysis Inhibition of human MATE1-mediated [14]-metformin uptake expressed in polarized MDCK2 cells after 5 mins by liquid scintillation counting analysis	[PMID: 23241029]
MES-SA	IC₅₀	0.003 μM Compound: Mx	Antiproliferative activity against MES-SA cells by MTT assay after 72 hrs Antiproliferative activity against MES-SA cells by MTT assay after 72 hrs	[PMID: 16824751]
MES-SA	IC₅₀	0.006 μM Compound: Mit	Antiproliferative activity against human MES-SA cells after 72 hrs by MTT assay Antiproliferative activity against human MES-SA cells after 72 hrs by MTT assay	[PMID: 25998504]
MES-SA	IC₅₀	0.012 μM Compound: Mx	Cytotoxicity against human MES-SA cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay Cytotoxicity against human MES-SA cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay	[PMID: 21444205]
MES-SA/Dx5	IC₅₀	0.028 μM Compound: Mx	Antiproliferative activity against doxorubicin-resistant MES-SA/Dx5 cells by MTT assay after 72 hrs Antiproliferative activity against doxorubicin-resistant MES-SA/Dx5 cells by MTT assay after 72 hrs	[PMID: 16824751]
MES-SA/Dx5	IC₅₀	0.073 μM Compound: Mit	Antiproliferative activity against human MES-SA/Dx5 cells after 72 hrs by MTT assay Antiproliferative activity against human MES-SA/Dx5 cells after 72 hrs by MTT assay	[PMID: 25998504]
MES-SA/Dx5	IC₅₀	0.08 μM Compound: Mx	Cytotoxicity against human MES-SA/Dx5 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay Cytotoxicity against human MES-SA/Dx5 cells assessed as inhibition of cell proliferation after 72 hrs by MTT assay	[PMID: 21444205]
MKN-28	IC₅₀	0.02 μg/mL Compound: mitoxantrone	Cytotoxicity against human MKN28 cells after 48 hrs by MTT assay Cytotoxicity against human MKN28 cells after 48 hrs by MTT assay	[PMID: 10843567]
MKN-45	IC₅₀	12 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human MKN45 cancer cell line was determined after 144 hr Cytotoxicity against human MKN45 cancer cell line was determined after 144 hr	[PMID: 15456268]
NCI-H460	EC₅₀	0.03 μM Compound: Mitoxantrone	Cytotoxicity against human NCI-H460 cells after 48 hrs by resazurin dye assay Cytotoxicity against human NCI-H460 cells after 48 hrs by resazurin dye assay	[PMID: 23988351]
NCI-H460	EC₅₀	0.12 μM Compound: mitoxantrone	Antiproliferative activity against human NCI-H460 cells after 48 hrs Antiproliferative activity against human NCI-H460 cells after 48 hrs	[PMID: 23395656]
NCI-H460	EC₅₀	0.1224 μg/mL Compound: Mitoxantrone	Antiproliferative activity against human NCI-H460 cells after 48 hrs by XTT assay Antiproliferative activity against human NCI-H460 cells after 48 hrs by XTT assay	[PMID: 21458262]
NCI-H460	IC₅₀	0.36 μM Compound: MX	Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell viability incubated for 2 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as inhibition of cell viability incubated for 2 hrs by MTT assay	[PMID: 31302589]
NCI-H460	GI₅₀	1.29 μM Compound: MX, mitoxantrone	Cytotoxicity against human NCI-H460 cells after 48 hrs by SRB assay Cytotoxicity against human NCI-H460 cells after 48 hrs by SRB assay	[PMID: 17962028]
NIH3T3	IC₅₀	42 μM Compound: Mitoxantrone	Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability after 24 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 28222315]
OVCAR-3	IC₅₀	5.8 nM Compound: Mitoxantrone	Anti-tumor activity against human OVCAR-3 ovarian cancer cell lines by using MTT assay Anti-tumor activity against human OVCAR-3 ovarian cancer cell lines by using MTT assay	[PMID: 7699715]
OVCAR-3	IC₅₀	6 nM Compound: mitoxantrone	Tested for inhibitory activity against human tumor cell line OVCAR-3, a resistant ovarian carcinoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line OVCAR-3, a resistant ovarian carcinoma using sulforhodamine B assay.	[PMID: 10956214]
P388	IC₅₀	4.3 nM Compound: Mitoxantrone	Antiproliferative activity against P388 cells by ELISA Antiproliferative activity against P388 cells by ELISA	[PMID: 17154505]
P388/ADR	IC₅₀	194 nM Compound: Mitoxantrone	Antiproliferative activity against adriamycin resistant P388 cells in presence of 5 uM 1,13-bis[4'-((5,7-dihydroxy)-4H-chromen-4-on-2-yl)phenyl]-1,4,7,10,13-pentaoxatridecane by ELISA Antiproliferative activity against adriamycin resistant P388 cells in presence of 5 uM 1,13-bis[4'-((5,7-dihydroxy)-4H-chromen-4-on-2-yl)phenyl]-1,4,7,10,13-pentaoxatridecane by ELISA	[PMID: 17154505]
P388/ADR	IC₅₀	395 nM Compound: Mitoxantrone	Antiproliferative activity against adriamycin resistant P388 cells by ELISA Antiproliferative activity against adriamycin resistant P388 cells by ELISA	[PMID: 17154505]
PC-3	IC₅₀	0.29 μM Compound: Mitoxantrone	Cytotoxicity against human PC3 cells after 48 hrs by SRB assay Cytotoxicity against human PC3 cells after 48 hrs by SRB assay	[PMID: 22100139]
PC-3	GI₅₀	0.78 μM Compound: MX, mitoxantrone	Cytotoxicity against human PC3 cells after 48 hrs by SRB assay Cytotoxicity against human PC3 cells after 48 hrs by SRB assay	[PMID: 17962028]
PC-3	IC₅₀	7 nM Compound: MX (Mitoxantrone)	Cytotoxicity against human PC3 cancer cell line was determined after 144 hr Cytotoxicity against human PC3 cancer cell line was determined after 144 hr	[PMID: 15456268]
SF-268	EC₅₀	0.315 μg/mL Compound: Mitoxantrone	Antiproliferative activity against human SF268 cells after 48 hrs by XTT assay Antiproliferative activity against human SF268 cells after 48 hrs by XTT assay	[PMID: 21458262]
SF-268	EC₅₀	0.32 μM Compound: mitoxantrone	Antiproliferative activity against human SF268 cells after 48 hrs Antiproliferative activity against human SF268 cells after 48 hrs	[PMID: 23395656]
SF-268	GI₅₀	0.97 μM Compound: MX, mitoxantrone	Cytotoxicity against human SF268 cells after 48 hrs by SRB assay Cytotoxicity against human SF268 cells after 48 hrs by SRB assay	[PMID: 17962028]
SGC-7901	IC₅₀	0.293 nM Compound: Mitoxantrone	Antiproliferative activity against human SGC7901 cells after 72 hrs by sulforhodamine B assay Antiproliferative activity against human SGC7901 cells after 72 hrs by sulforhodamine B assay	[PMID: 30485090]
SK-OV-3	IC₅₀	0.0053 μM Compound: Mitoxantrone	Concentration required to inhibit SKOV-3-cell growth by 50% Concentration required to inhibit SKOV-3-cell growth by 50%	[PMID: 9703471]
SK-OV-3	EC₅₀	0.12 μM Compound: mitoxantrone	Antiproliferative activity against human SKOV3 cells after 48 hrs Antiproliferative activity against human SKOV3 cells after 48 hrs	[PMID: 23395656]
SK-OV-3	EC₅₀	0.1207 μg/mL Compound: Mitoxantrone	Antiproliferative activity against human SKOV3 cells after 48 hrs by XTT assay Antiproliferative activity against human SKOV3 cells after 48 hrs by XTT assay	[PMID: 21458262]
SK-OV-3	GI₅₀	11 μM Compound: Mitoxantrone	Growth inhibition of human SKOV3 cells by MTT assay Growth inhibition of human SKOV3 cells by MTT assay	[PMID: 20153184]
SK-OV-3	IC₅₀	9.8 μM Compound: Mitoxantrone	Cytotoxicity against human SKOV3 cells by MTT assay Cytotoxicity against human SKOV3 cells by MTT assay	[PMID: 19615900]
U-937	IC₅₀	6.2 μM Compound: Mitoxantrone	Cytotoxicity against human U937 cells by MTT assay Cytotoxicity against human U937 cells by MTT assay	[PMID: 19615900]
UACC-375	IC₅₀	48 nM Compound: Mitoxantrone	Anti-tumor activity against human UACC375 melanoma cell lines by using MTT assay Anti-tumor activity against human UACC375 melanoma cell lines by using MTT assay	[PMID: 7699715]
UACC-375	IC₅₀	48 nM Compound: mitoxantrone	Tested for inhibitory activity against human tumor cell line UA375 of melanoma using sulforhodamine B assay. Tested for inhibitory activity against human tumor cell line UA375 of melanoma using sulforhodamine B assay.	[PMID: 10956214]
WiDr	IC₅₀	488 nM Compound: mitoxantrone	Tested for inhibitory activity against a multi drug resistant (MDR) strain of human colon carcinoma WiDr using sulforhodamine B assay (it is MRP positive but non-P-glycoprotein). Tested for inhibitory activity against a multi drug resistant (MDR) strain of human colon carcinoma WiDr using sulforhodamine B assay (it is MRP positive but non-P-glycoprotein).	[PMID: 10956214]
WiDr	IC₅₀	8 nM Compound: mitoxantrone	Tested for inhibitory activity against a sensitive strain of human colon carcinoma WiDr using sulforhodamine B assay (it is MRP positive but non-P-glycoprotein). Tested for inhibitory activity against a sensitive strain of human colon carcinoma WiDr using sulforhodamine B assay (it is MRP positive but non-P-glycoprotein).	[PMID: 10956214]

體外研究
(In Vitro)

Mitoxantrone 對組蛋白 H1 競爭性抑制 PKC，其 K_i 值為 6.3 μM，對磷脂酰絲氨酸和 ATP 非競爭性抑制^[1]。
Mitoxantrone (0.5 μg/mL，48 h) 誘導 B-CLL 細胞減少。Mitoxantrone 誘導 DNA 片段化和聚 (ADP-核糖) 聚合酶 (PARP) 的蛋白水解切割，證明 Mitoxantrone 的細胞毒性作用是由于誘導細胞凋亡^[2]。
Mitoxantrone 表明對人乳腺癌細胞系 MDA-MB-231 和 MCF-7 的細胞毒性，IC₅₀ 值分別為 18 和 196 nM^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

Mitoxantrone (IP，0-3.2 mg/kg/天) 在腹腔注射；植入 L1210 白血病的小鼠中以 1.6 mg/kg 產(chǎn)生顯著數(shù)量的 60 天存活率^[4]。
Mitoxantrone (IV，0-3.2 mg/kg/天) 顯示出有效的抗腫瘤活性，并在皮下注射；植入的 Lewis 肺癌中以 3.2 mg/kg 產(chǎn)生 60% 的 ILS (壽命延長)^[4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

444.48

Formula

C₂₂H₂₈N₄O₆

CAS 號

65271-80-9

性狀

固體

顏色

Brown to black

中文名稱

米托蒽醌；二羥蒽二酮

結(jié)構(gòu)分類

初始來源

內(nèi)源性代謝物

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)

細胞實驗：

DMSO 中的溶解度 : 35 mg/mL (78.74 mM; 超聲助溶 (<60°C); 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	2.2498 mL	11.2491 mL	22.4982 mL
5 mM	0.4500 mL	2.2498 mL	4.4996 mL
10 mM	0.2250 mL	1.1249 mL	2.2498 mL

查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

摩爾計算器
稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動物實驗：

請根據(jù)您的實驗動物和給藥方式選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

方案一

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.68 mM); 澄清溶液

此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.68 mM); 澄清溶液

此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液中，混合均勻。
2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

動物溶解方案計算器

請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

每只動物的給藥體積

μL

動物數(shù)量

只

由于實驗過程有損耗，建議您多配一只動物的量

請輸入您的動物體內(nèi)配方組成：

DMSO +

Tween-80 +

Saline

如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。

方案所需 助溶劑 包括：DMSO，，均可在 MCE 網(wǎng)站選購。，Tween 80，均可在 MCE 網(wǎng)站選購。

計算結(jié)果

工作液所需濃度 : mg/mL

儲備液配制方法 : mg 藥物溶于 μL DMSO（母液濃度為 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。

動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。

連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。

請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱（超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。

純度 & 產(chǎn)品資料

純度: 98.70%

選擇批次：

Data Sheet (647 KB) SDS (393 KB)

COA (288 KB) HNMR (271 KB) LCMS (95 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻

[1]. Takeuchi N, et al. Inhibitory effect of mitoxantrone on activity of protein kinase C and growth of HL60 cells. J Biochem. 1992 Dec;112(6):762-7. [Content Brief]

[2]. Bellosillo B, et al. Mitoxantrone, a topoisomerase II inhibitor, induces apoptosis of B-chronic lymphocytic leukaemia cells. Br J Haematol. 1998 Jan;100(1):142-6. [Content Brief]

[3]. Vibet S, et al. Differential subcellular distribution of mitoxantrone in relation to chemosensitization in two human breast cancer cell lines. Drug Metab Dispos. 2007 May;35(5):822-8. [Content Brief]

[4]. Fujimoto S, et al. Antitumor activity of mitoxantrone against murine experimental tumors: comparative analysis against various antitumor antibiotics. Cancer Chemother Pharmacol. 1982;8(2):157-62. [Content Brief]

[5]. Sharon E Altmann, et al. Inhibition of cowpox virus and monkeypox virus infection by mitoxantrone. Antiviral Res. 2012 Feb;93(2):305-308. [Content Brief]

Cell Assay ^[3]	The human breast carcinoma cell lines MDA-MB-231 and MCF-7 are seeded in standard 96-well plates. One day after seeding, the culture medium is changed and replaced by medium containing different concentration of Mitoxantrone (10^-5 to 5 μM) with or without DHA (30 μM) during 7 days. Viability of cells are measured as a whole by the tetrazolium salt assay^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[4]	Mice: Mitoxantrone is tested for antitumor activity against experimental tumors in mice and the results are compared with those of seven antitumor antibiotics. The drugs are given IP or IV, in general on days 1, 5, and 9 following tumor inoculation. Mitoxantrone is given IP at the optimal dose (1.6 mg/kg/day; as a free base)^[4]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻	[1]. Takeuchi N, et al. Inhibitory effect of mitoxantrone on activity of protein kinase C and growth of HL60 cells. J Biochem. 1992 Dec;112(6):762-7. [Content Brief] [2]. Bellosillo B, et al. Mitoxantrone, a topoisomerase II inhibitor, induces apoptosis of B-chronic lymphocytic leukaemia cells. Br J Haematol. 1998 Jan;100(1):142-6. [Content Brief] [3]. Vibet S, et al. Differential subcellular distribution of mitoxantrone in relation to chemosensitization in two human breast cancer cell lines. Drug Metab Dispos. 2007 May;35(5):822-8. [Content Brief] [4]. Fujimoto S, et al. Antitumor activity of mitoxantrone against murine experimental tumors: comparative analysis against various antitumor antibiotics. Cancer Chemother Pharmacol. 1982;8(2):157-62. [Content Brief] [5]. Sharon E Altmann, et al. Inhibition of cowpox virus and monkeypox virus infection by mitoxantrone. Antiviral Res. 2012 Feb;93(2):305-308. [Content Brief]

Mitoxantrone 相關(guān)分類

完整儲備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2498 mL	11.2491 mL	22.4982 mL	56.2455 mL
	5 mM	0.4500 mL	2.2498 mL	4.4996 mL	11.2491 mL
	10 mM	0.2250 mL	1.1249 mL	2.2498 mL	5.6245 mL
	15 mM	0.1500 mL	0.7499 mL	1.4999 mL	3.7497 mL
	20 mM	0.1125 mL	0.5625 mL	1.1249 mL	2.8123 mL
	25 mM	0.0900 mL	0.4500 mL	0.8999 mL	2.2498 mL
	30 mM	0.0750 mL	0.3750 mL	0.7499 mL	1.8748 mL
	40 mM	0.0562 mL	0.2812 mL	0.5625 mL	1.4061 mL
	50 mM	0.0450 mL	0.2250 mL	0.4500 mL	1.1249 mL
	60 mM	0.0375 mL	0.1875 mL	0.3750 mL	0.9374 mL

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Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Mitoxantrone (Synonyms: 米托蒽醌; Mitozantrone; NSC 301739)

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Other Forms of Mitoxantrone:

Mitoxantrone 相關(guān)抗體

MCE 顧客使用本產(chǎn)品發(fā)表的 26 篇科研文獻

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查看 Topoisomerase 亞型特異性產(chǎn)品:

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