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  2. Cell Cycle/DNA Damage Antibody-drug Conjugate/ADC Related Anti-infection Autophagy Apoptosis
  3. Topoisomerase DNA/RNA Synthesis ADC Cytotoxin Bacterial Autophagy Apoptosis Antibiotic
  4. Daunorubicin hydrochloride

Daunorubicin hydrochloride  (Synonyms: 鹽酸柔紅霉素; Daunomycin hydrochloride; RP 13057 hydrochloride; Rubidomycin hydrochloride)

目錄號(hào): HY-13062 純度: 99.67%
COA 產(chǎn)品使用指南 技術(shù)支持

Daunorubicin (Daunomycin) hydrochloride 是一種拓?fù)洚悩?gòu)酶 II (topoisomerase II) 抑制劑，具有有效的抗腫瘤活性。Daunorubicin hydrochloride 抑制 DNA 和 RNA 合成 (DNA and RNA synthesis)。Daunorubicin hydrochloride 是一種細(xì)胞毒素，可抑制癌細(xì)胞活力并誘導(dǎo)細(xì)胞凋亡 (apoptosis) 和壞死 (necrosis)。Daunorubicin hydrochloride 還是一種蒽環(huán)類抗生素。Daunorubicin hydrochloride 可用于研究感染和多種癌癥，包括白血病、非霍奇金淋巴瘤、尤文氏肉瘤、維爾姆斯氏瘤。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Daunorubicin hydrochloride Chemical Structure

Daunorubicin hydrochloride Chemical Structure

CAS No. : 23541-50-6

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥567
In-stock
5 mg ￥321
In-stock
10 mg ￥515
In-stock
50 mg ￥1600
In-stock
100 mg ￥2500
In-stock
200 mg ￥3500
In-stock
500 mg ￥5600
In-stock
1 g   詢價(jià)  
5 g   詢價(jià)  

* Please select Quantity before adding items.

Customer Review

Other Forms of Daunorubicin hydrochloride:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Daunorubicin (Daunomycin) hydrochloride is a topoisomerase II inhibitor with potent anti-tumor activity. Daunorubicin hydrochloride inhibits DNA and RNA synthesis. Daunorubicin hydrochloride is a cytotoxin that inhibits cancer cell viability and induces apoptosis and necrosis. Daunorubicin hydrochloride is also an anthracycline antibiotic. Daunorubicin hydrochloride can be used in the research of infection and variety of cancers, including leukemia, non-Hodgkin lymphomas, Ewing's sarcoma, Wilms' tumor[1][2][4][5].

IC50 & Target[1][2]

Topoisomerase II

 

Daunorubicins/Doxorubicins

 

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
CAPAN-1 IC50
3.1 μM
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against Capen-1 cell line (pancreatic carcinoma) after incubation for 72 hour
Cytotoxicity of compound was tested against Capen-1 cell line (pancreatic carcinoma) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
CAPAN-1 IC50
3.9 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against Capan-1 cell line (pancreatic carcinoma) after incubation for 24 hour
Cytotoxicity of compound was tested against Capan-1 cell line (pancreatic carcinoma) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
CHO IC50
2.94 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 72 hour
Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
CHO IC50
3.9 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 24 hour
Cytotoxicity of compound was tested against CHO cell line (Chinese hamster ovary) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
HCT-116 IC50
0.28 μg/mL
Compound: Daunorubicin HCl
Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay
Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay
[PMID: 12141872]
HeLa IC50
0.32 μM
Compound: Daunorubicin
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
Cytotoxicity against human HeLa cells after 48 hrs by MTT assay
[PMID: 17190447]
HL-60 IC50
< 9.8 x 10-8 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 24 hour
Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
HL-60 IC50
9.8 x 10-8 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 72 hour
Cytotoxicity of compound was tested against HL-60 cell line (promyelocytic leukemia) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
HMEC IC50
< 9.8 x 10-8 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 72 hour
Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
HMEC IC50
3.9 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 24 hour
Cytotoxicity of compound was tested against HMEC cell line (human mamm epithelial cells) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
HRT-18 cell line IC50
0.11 μg/mL
Compound: Daunorubicin HCl
Cytotoxicity against human HRT18 cells after 48 hrs by MTT assay
Cytotoxicity against human HRT18 cells after 48 hrs by MTT assay
[PMID: 12141872]
HT-29 IC50
0.072 μg/mL
Compound: Daunorubicin HCl
Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay
Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay
[PMID: 12141872]
HT-29 IC50
3.57 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 72 hour
Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
HT-29 IC50
7.8 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 24 hour
Cytotoxicity of compound was tested against HT-29 cell line (colon carcinoma) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
K562 IC50
> 5 μM
Compound: DNR
Cytotoxicity against doxorubicin-resistant K562 cell line by MTS assay
Cytotoxicity against doxorubicin-resistant K562 cell line by MTS assay
[PMID: 16509594]
K562 IC50
0.015 μM
Compound: DNR
Cytotoxicity against drug-sensitive K562 cell line by MTS assay
Cytotoxicity against drug-sensitive K562 cell line by MTS assay
[PMID: 16509594]
K562 IC50
156.6 μM
Compound: DNR hydrochloride
Cytotoxicity against K562 leukemia cell line done for 72 hr at 37 degree C with compound in MTS assay
Cytotoxicity against K562 leukemia cell line done for 72 hr at 37 degree C with compound in MTS assay
[PMID: 16078845]
LNCaP IC50
0.011 μg/mL
Compound: Daunorubicin HCl
Cytotoxicity against human LNCAP cells expressing androgen receptor after 48 hrs by MTT assay
Cytotoxicity against human LNCAP cells expressing androgen receptor after 48 hrs by MTT assay
[PMID: 12141872]
MCF7 IC50
0.12 μg/mL
Compound: Daunorubicin HCl
Cytotoxicity against human MCF7 cells expressing estrogen receptor after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells expressing estrogen receptor after 48 hrs by MTT assay
[PMID: 12141872]
MCF7 IC50
0.22 μM
Compound: Daunorubicin
Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 48 hrs by MTT assay
[PMID: 17190447]
MCF7 IC50
1.35 x 10-5 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 72 hour
Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
MCF7 IC50
7.8 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 24 hour
Cytotoxicity of compound was tested against MCF-7 cell line (breast carcinoma) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
MDA-MB-231 IC50
0.25 μM
Compound: Daunorubicin
Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay
[PMID: 17190447]
MOLT-4 IC50
1 nM
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 24 hour
Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
MOLT-4 IC50
8.55 x 10-13 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 72 hour
Cytotoxicity of compound was tested against Molt-4 cell line (T-cell leukemia) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
NHDF IC50
1.9 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 24 hour
Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
NHDF IC50
4.04 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 72 hour
Cytotoxicity of compound was tested against NHDF cell line (norm human dermal fibroblast) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
OVCAR-3 IC50
2.87 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 72 hour
Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
OVCAR-3 IC50
3.9 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 24 hour
Cytotoxicity of compound was tested against Ovcar-3 cell line (ovarian carcinoma) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
P388 IC50
< 9.8 x 10-8 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 72 hour
Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
P388 IC50
3.9 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 24 hour
Cytotoxicity of compound was tested against P-388 cell line (mouse leukemia) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
PC-3 IC50
0.23 μg/mL
Compound: Daunorubicin HCl
Cytotoxicity against human PC3 cells after 48 hrs by MTT assay
Cytotoxicity against human PC3 cells after 48 hrs by MTT assay
[PMID: 12141872]
PC-3 IC50
0.44 μM
Compound: Daunorubicin
Cytotoxicity against human PC-3 cells after 48 hrs by MTT assay
Cytotoxicity against human PC-3 cells after 48 hrs by MTT assay
[PMID: 17190447]
SK-MEL-28 IC50
1.41 μM
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 72 hour
Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 72 hour
10.1016/S0960-894X(00)80285-1
SK-MEL-28 IC50
1.6 μM
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 24 hour
Cytotoxicity of compound was tested against SK-Mel-28 cell line (melanoma) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
SW-620 IC50
8.6 μM
Compound: DNR hydrochloride
Cytotoxicity against SW620 colon cancer cell line done for 72 h at 37 degree C with compound in MTS assay
Cytotoxicity against SW620 colon cancer cell line done for 72 h at 37 degree C with compound in MTS assay
[PMID: 16078845]
UCLA P-3 IC50
7.3 x 10-7 M
Compound: 1 (Daunomycin hydrochloride)
Cytotoxicity of compound was tested against UCLA P-3 cell line (lung carcinoma) after incubation for 24 hour
Cytotoxicity of compound was tested against UCLA P-3 cell line (lung carcinoma) after incubation for 24 hour
10.1016/S0960-894X(00)80285-1
體外研究
(In Vitro)

Daunorubicin hydrochloride (0-256 μg/mL; 30 mins) 可抑制敏感和耐藥的艾氏腹水瘤細(xì)胞中的 DNA 和 RNA 合成[2]。
Daunorubicin hydrochloride (7 nM-1.9 μM，72 h) 在 Molt-4 細(xì)胞和 L3.6 細(xì)胞中表現(xiàn)出化療敏感性[3][4]。
Daunorubicin hydrochloride (0.4 μM，48 小時(shí)) 可誘導(dǎo) L3.6 細(xì)胞凋亡和壞死[4]。
Daunorubicin hydrochloride (0.4 μM，120 mins) 可誘導(dǎo) L3.6 細(xì)胞中的活性氧 (ROS) 生成[4]。
Daunorubicin hydrochloride (2 μM，24 小時(shí)）可誘導(dǎo) K562 細(xì)胞 (髓系細(xì)胞系) 的自噬[6]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[3][4]

Cell Line: Molt-4 cells (a human T-lymphoblastic leukemia cell line), L3.6 cells (metastatic human pancreatic cell line)
Concentration: 7 nM-1.9 μM
Incubation Time: 72 h
Result: Inhibited cell viability with IC50 values of 40 nM (Molt-4) and 400 nM (L3.6).

Apoptosis Analysis[4]

Cell Line: L3.6 cells
Concentration: 0.4 μM
Incubation Time: 72 h
Result: Induced necrosis without apoptosis at 24 h, induced both an apoptotic and extensive necrotic response at 48 h.

Western Blot Analysis[6]:

Cell Line: K562 cells
Concentration: 2 μM
Incubation Time: 24 h
Result: Enabled the switch of LC3-I into LC3-II, accompanied with a significant increased expression level of LC3.
體內(nèi)研究
(In Vivo)

Daunorubicin hydrochloride (i.v.; 3 mg/kg，每次注射三次，間隔 48 小時(shí)) 對(duì)大鼠產(chǎn)生心臟毒性和腎毒性[5]。
Daunorubicin hydrochloride (i.p.; 10 mg/kg）可誘導(dǎo)小鼠姊妹染色單體交換[7]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats[5]
Dosage: 3 mg/kg
Administration: Intravenous injection, three times at 48 h intervals.
Result: Caused a significant increase in MDA (malondialdehyde) level in renal tissue, accompanied by a significant reduction in total GPx activity.
Increased urinary protein excretion, serum creatinine, and BUN level.
Clinical Trial
分子量

563.98

Formula

C27H30ClNO10
CAS 號(hào)
性狀

固體

顏色

Pink to red

中文名稱

鹽酸柔紅霉素；鹽酸佐柔比星；道諾霉素鹽酸鹽；鹽酸正定霉素；鹽酸紅比霉素
結(jié)構(gòu)分類
初始來(lái)源

Streptomyces coeruleorubidus
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 50 mg/mL (88.66 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

H2O 中的溶解度 : ≥ 34 mg/mL (60.29 mM)

* "≥" means soluble, but saturation unknown.

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 1.7731 mL 8.8656 mL 17.7311 mL
5 mM 0.3546 mL 1.7731 mL 3.5462 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

* 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn)：

請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

  • 方案 一

    請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.69 mM); 澄清溶液

    此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.69 mM); 澄清溶液

    此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。

以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶。

  • 方案 一

    請(qǐng)依序添加每種溶劑： PBS

    Solubility: 33.33 mg/mL (59.10 mM); 澄清溶液; 超聲助溶

動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
該產(chǎn)品水溶性佳，請(qǐng)具體參考實(shí)測(cè) 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
純度 & 產(chǎn)品資料

純度: 99.67%

參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.7731 mL 8.8656 mL 17.7311 mL 44.3278 mL
5 mM 0.3546 mL 1.7731 mL 3.5462 mL 8.8656 mL
10 mM 0.1773 mL 0.8866 mL 1.7731 mL 4.4328 mL
15 mM 0.1182 mL 0.5910 mL 1.1821 mL 2.9552 mL
20 mM 0.0887 mL 0.4433 mL 0.8866 mL 2.2164 mL
25 mM 0.0709 mL 0.3546 mL 0.7092 mL 1.7731 mL
30 mM 0.0591 mL 0.2955 mL 0.5910 mL 1.4776 mL
40 mM 0.0443 mL 0.2216 mL 0.4433 mL 1.1082 mL
50 mM 0.0355 mL 0.1773 mL 0.3546 mL 0.8866 mL
60 mM 0.0296 mL 0.1478 mL 0.2955 mL 0.7388 mL
DMSO 80 mM 0.0222 mL 0.1108 mL 0.2216 mL 0.5541 mL

* 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過(guò)濾除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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