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  2. Anti-infection Membrane Transporter/Ion Channel Apoptosis
  3. Bacterial Fungal Na+/K+ ATPase Apoptosis Antibiotic
  4. Bafilomycin C1

Bafilomycin C1  (Synonyms: 巴菲霉素C1)

目錄號(hào): HY-130173 純度: ≥99.0%
COA 產(chǎn)品使用指南 技術(shù)支持

Bafilomycin C1 是從鏈霉菌中分離得到的大環(huán)內(nèi)酯類抗生素 (antibiotic)。Bafilomycin C1 是一種有效的、特異性的、可逆的 vacuolar-type H+-ATPases 抑制劑。Bafilomycin C1 抑制革蘭氏陽性菌 (bacteria) 和真菌 (fungi) 的生長。Bafilomycin C1 可誘導(dǎo)細(xì)胞凋亡 (apoptosis)，可用于肝細(xì)胞癌 (HCC) 的研究。

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Bafilomycin C1 Chemical Structure

Bafilomycin C1 Chemical Structure

CAS No. : 88979-61-7

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MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻(xiàn)

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Bafilomycin C1 is a macrolide antibiotic isolated from Streptomyces sp. Bafilomycin C1 is a potent, specific and reversible inhibitor of vacuolar-type H+-ATPases (V-ATPases). Bafilomycin C1 inhibits growth of gram-positive bacteria and fungi[2]. Bafilomycin C1 induces cell apoptosis and can be used for the study of hepatocellular carcinoma (HCC)[2].

IC50 & Target

Macrolide

 

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
BGC-823 IC50
1.83 μM
Compound: 7
Cytotoxicity against human BGC823 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human BGC823 cells assessed as reduction in cell viability by MTT assay
[PMID: 26933756]
Caco-2 IC50
1.89 μM
Compound: 7
Cytotoxicity against human Caco2 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human Caco2 cells assessed as reduction in cell viability by MTT assay
[PMID: 26933756]
NCI-H460 IC50
3.43 μM
Compound: 7
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability by MTT assay
[PMID: 26933756]
SMMC-7721 IC50
0.54 μM
Compound: 7
Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay
Cytotoxicity against human SMMC7721 cells assessed as reduction in cell viability by MTT assay
[PMID: 26933756]
體外研究
(In Vitro)

Bafilomycin C1 (0.33-10 μM；6 days) 抑制 SMMC7721 和 HepG2 細(xì)胞的生長和增殖，呈時(shí)間和劑量依賴性[2]。
Bafilomycin C1 (0.33-3.3 μM；24 h) 降低 SMMC7721 細(xì)胞中 cyclin D3、cyclin E1、CDK2、CDK4 和 CDK6 的 mRNA 和蛋白表達(dá)[2]。
Bafilomycin C1 (3.3-10 μM；24 小時(shí)) 導(dǎo)致形態(tài)學(xué)改變并通過 Hoechst 33258 (HY-15558) 染色增加凋亡細(xì)胞的數(shù)量[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: SMMC7721 cell and HepG2 cell
Concentration: 0.33 μM, 1.1 μM, and 3.3 μM for SMMC7721
1.1 μM, 3.3 μM, and 10.0 μM for HepG2
Incubation Time: 6 days
Result: Retarded the cell growth.

Western Blot Analysis[2]

Cell Line: SMMC7721 cells
Concentration: 3.3 μM
Incubation Time: 24 hours
Result: Decreaed cyclin D3/E1,CDK2/4/6 protein expression and increased p21.

Apoptosis Analysis[2]

Cell Line: SMMC7721 and HepG2 cells
Concentration: 3.3 μM; 10 μM
Incubation Time: 24 hours
Result: Induced apoptosis in SMMC7721 and HepG2 cells.
體內(nèi)研究
(In Vivo)

Bafilomycin C1 (皮下注射；0.2 mg/kg；20 天) 在裸鼠模型中抑制腫瘤生長，無明顯不良反應(yīng)或副作用[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice (weighing 18-20 g) subcutaneous injected by SMMC7721 cell suspension (5×106 cells/100 μL)[2]
Dosage: 0.2 mg/kg
Administration: Subcutaneous injection; 20 days
Result: Suppressed tumor growth of SMMC7721 tumor xenografts.
分子量

720.89

Formula

C39H60O12
CAS 號(hào)
性狀

固體

顏色

White to off-white

中文名稱

巴菲霉素C1
結(jié)構(gòu)分類
初始來源

Streptomyces sp
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 5 mg/mL (6.94 mM; 超聲加熱助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 1.3872 mL 6.9359 mL 13.8717 mL
5 mM 0.2774 mL 1.3872 mL 2.7743 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn)：

以下溶解方案源自文獻(xiàn)，僅供參考，建議您先取少量樣品進(jìn)行嘗試。

  • 方案 一

    Bafilomycin C1 (0.2 mg/kg body weight, dissolved in 60% 1,2-propanediol)[2]

動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料

純度: ≥99.0%

參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3872 mL 6.9359 mL 13.8717 mL 34.6794 mL
5 mM 0.2774 mL 1.3872 mL 2.7743 mL 6.9359 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Bafilomycin C1
目錄號(hào)：
HY-130173
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