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  2. Immunology/Inflammation
  3. NO Synthase
  4. L-NIL

L-NIL 是誘導(dǎo)型一氧化氮合成酶 (iNOS) 的抑制劑，其對(duì) miNOS 的 IC50 值為3.3 μM。

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L-NIL Chemical Structure

L-NIL Chemical Structure

CAS No. : 53774-63-3

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in Water ￥990
In-stock
50 mg ￥900
In-stock
100 mg ￥1600
In-stock
200 mg   詢價(jià)  
500 mg   詢價(jià)  

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Customer Review

Other Forms of L-NIL:

    L-NIL purchased from MCE. Usage Cited in: J Adv Res. 5 March 2022.

    Inhibiting iNOS with L-NIL (10 μM) blunted the adverse effect of iNOS expression in cardiomyocytes.

    查看 NO Synthase 亞型特異性產(chǎn)品:

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    L-NIL is an inducible NO synthase inhibitor, with an IC50 of 3.3 μM for miNOS[1][2][3].

    IC50 & Target

    iNOS

     

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    DLD-1 IC50
    0.13 μM
    Compound: 3
    Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells
    Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells
    		[PMID: 11327580]
    DLD-1 IC50
    2.8 μM
    Compound: 3
    Inhibitory activity against synthesis of inducible nitric oxide synthase by DLD-1 cells
    Inhibitory activity against synthesis of inducible nitric oxide synthase by DLD-1 cells
    		[PMID: 12620067]
    DLD-1 IC50
    280 nM
    Compound: 1
    Inhibition of human iNOS expressed in human DLD1 cells after 1 hr
    Inhibition of human iNOS expressed in human DLD1 cells after 1 hr
    		[PMID: 18024030]
    DLD-1 IC50
    31000 nM
    Compound: 1
    Inhibition of cytokine-induced iNOS activity in human DLD1 cells after 24 hrs
    Inhibition of cytokine-induced iNOS activity in human DLD1 cells after 24 hrs
    		[PMID: 18024030]
    HUVEC IC50
    2.4 μM
    Compound: 3
    Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells
    Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells
    		[PMID: 11327580]
    RAW264.7 IC50
    > 200 μM
    Compound: L-NIL
    Cytotoxicity against LPS-stimulated mouse RAW264.7 cells by Griess method
    Cytotoxicity against LPS-stimulated mouse RAW264.7 cells by Griess method
    		[PMID: 14510614]
    RAW264.7 IC50
    1.55 μM
    Compound: NIL
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells pretreated for 1 hr followed by LPS-stimulation and measured after 24 hrs by Griess assay
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells pretreated for 1 hr followed by LPS-stimulation and measured after 24 hrs by Griess assay
    		[PMID: 29932657]
    RAW264.7 IC50
    15.8 μM
    Compound: L-NIL
    Inhibition of LPS-stimulated nitric oxide production in rat RAW264.7 cells after 24 hrs by Griess assay
    Inhibition of LPS-stimulated nitric oxide production in rat RAW264.7 cells after 24 hrs by Griess assay
    		[PMID: 29799195]
    RAW264.7 IC50
    15.8 μM
    Compound: L-NIL
    Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay
    		[PMID: 25666824]
    RAW264.7 IC50
    18.6 μM
    Compound: L-NIL
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method
    Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells by Griess method
    		[PMID: 14510614]
    RAW264.7 IC50
    20.14 μM
    Compound: L-NIL
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    Antiinflammatory activity against mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production after 24 hrs by Griess assay
    		[PMID: 24360561]
    RAW264.7 IC50
    28.29 μM
    Compound: L-NIL
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production measured after 24 hrs by Griess assay
    		[PMID: 29102229]
    RAW264.7 IC50
    7 μM
    Compound: L-Nil
    Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation and measured after 24 hrs by Griess reagent based colorimetric method
    Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation and measured after 24 hrs by Griess reagent based colorimetric method
    		[PMID: 30724564]
    RAW264.7 IC50
    7.5 μM
    Compound: L-NIL
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay
    Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation for 24 hrs by Griess assay
    		[PMID: 27588583]
    Sf9 IC50
    2500 nM
    Compound: 1
    Inhibition of human eNOS expressed in insect SF9 cells after 1 hr
    Inhibition of human eNOS expressed in insect SF9 cells after 1 hr
    		[PMID: 18024030]
    Sf9 IC50
    6000 nM
    Compound: 1
    Inhibition of human nNOS expressed in insect SF9 cells after 1 hr
    Inhibition of human nNOS expressed in insect SF9 cells after 1 hr
    		[PMID: 18024030]
    體外研究
    (In Vitro)

    L-NIL 對(duì)小鼠誘導(dǎo)型 NOS (miNOS) 和大鼠腦組成型 NOS (rcNOS) 均產(chǎn)生濃度依賴性抑制，并且對(duì) miNOS 的效力顯著增強(qiáng)。L-NIL 與 miNOS 和 rcNOS 的 IC50 值分別為 3.3 和 92 pM，表明 L-NIL 對(duì) miNOS 的選擇性高 28 倍。此外，L-NIL 對(duì) miNOS 的效力比 L-NMA 或 L-NNA 高約 6 倍[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    L-NIL (10 和 30 mg/kg，IP) 可預(yù)防小鼠腎臟 IR 誘導(dǎo)的炎癥、氧化應(yīng)激和自噬[1]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Adult male Balb/c (20-25 g)[1].
    Dosage: 10 and 30?mg/kg.
    Administration: Intraperitoneally at the end of CLP and at 6?h after sepsis induction.
    Result: Led to a negligible increase in plasma NGAL compared to sham mice.
    Led to a significant decrease in both TLR4 and IL1β protein contents and clusterin transcript.
    Showed an increase in NFAT5 mRNA levels, as compared with mice treated with vehicle.
    Promoted a decrease in AR protein expression, as compared with animals treated with vehicle.
    分子量

    187.24

    Formula

    C8H17N3O2
    CAS 號(hào)
    性狀

    固體

    顏色

    White to off-white

    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲(chǔ)存方式

    -20°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    H2O 中的溶解度 : 50 mg/mL (267.04 mM; 超聲助溶)

    DMSO 中的溶解度 : < 1 mg/mL (insoluble or slightly soluble)

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 5.3407 mL 26.7037 mL 53.4074 mL
    5 mM 1.0681 mL 5.3407 mL 10.6815 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    * 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物實(shí)驗(yàn)：

    以下溶解方案，請(qǐng)直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請(qǐng)依序添加每種溶劑： PBS

      Solubility: 100 mg/mL (534.07 mM); 澄清溶液; 超聲助溶

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    只
    由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    該產(chǎn)品水溶性佳，請(qǐng)具體參考實(shí)測(cè) 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
    您所需的儲(chǔ)備液濃度超過該產(chǎn)品的實(shí)測(cè)溶解度，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
    純度 & 產(chǎn)品資料

    純度: 99.82%

    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.3407 mL 26.7037 mL 53.4074 mL 133.5185 mL
    5 mM 1.0681 mL 5.3407 mL 10.6815 mL 26.7037 mL
    10 mM 0.5341 mL 2.6704 mL 5.3407 mL 13.3518 mL
    15 mM 0.3560 mL 1.7802 mL 3.5605 mL 8.9012 mL
    20 mM 0.2670 mL 1.3352 mL 2.6704 mL 6.6759 mL
    25 mM 0.2136 mL 1.0681 mL 2.1363 mL 5.3407 mL
    30 mM 0.1780 mL 0.8901 mL 1.7802 mL 4.4506 mL
    40 mM 0.1335 mL 0.6676 mL 1.3352 mL 3.3380 mL
    50 mM 0.1068 mL 0.5341 mL 1.0681 mL 2.6704 mL
    60 mM 0.0890 mL 0.4451 mL 0.8901 mL 2.2253 mL
    80 mM 0.0668 mL 0.3338 mL 0.6676 mL 1.6690 mL
    100 mM 0.0534 mL 0.2670 mL 0.5341 mL 1.3352 mL

    * 備注：如您選擇水作為儲(chǔ)備液，請(qǐng)稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Inquiry Information

    產(chǎn)品名稱：
    L-NIL
    目錄號(hào)：
    HY-12116
    需求量：