成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

  2. Cell Cycle/DNA Damage Cytoskeleton
  3. Microtubule/Tubulin
  4. Pironetin

Pironetin 是從鏈霉菌屬物種分離的 α/β 不飽和內(nèi)酯。Pironetin 與 α-tubulin 結(jié)合，是微管聚合的有效抑制劑，具有細(xì)胞周期停滯和抗腫瘤活性。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務(wù)

Pironetin Chemical Structure

Pironetin Chemical Structure

CAS No. : 151519-02-7

1.  客戶無需承擔(dān)相應(yīng)的運(yùn)輸費(fèi)用。

2.  同一機(jī)構(gòu)（單位）同一產(chǎn)品試用裝僅限申領(lǐng)一次，同一機(jī)構(gòu)（單位）一年內(nèi)

     可免費(fèi)申領(lǐng)三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
500 μg ￥4740
In-stock
1 mg 現(xiàn)貨 詢價
5 mg 現(xiàn)貨 詢價
10 mg   詢價  
50 mg   詢價  

* Please select Quantity before adding items.

Customer Review

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Pironetin is an α/β unsaturated lactone isolated from Streptomyces species. Pironetin binds to α-tubulin and is a potent inhibitor of microtubule polymerization, and has cell cycle arrest and antitumor activity[1][2].

IC50 & Target

Microtubule[1]
細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
A2780 IC50
0.0029 μM
Compound: Pironetin
Cytotoxicity against compound-sensitive human A2780 cells after 24 hrs by MTT assay
Cytotoxicity against compound-sensitive human A2780 cells after 24 hrs by MTT assay
[PMID: 21396747]
A2780 IC50
0.008 μM
Compound: pironetin
Cytotoxicity against human A2780 cells after 24 hrs by MTT assay
Cytotoxicity against human A2780 cells after 24 hrs by MTT assay
[PMID: 25426924]
A2780 GI50
22.2 nM
Compound: 1
Inhibition of tubulin alpha in human A2780 cells assessed as cell growth inhibition after 48 hrs by MTT assay
Inhibition of tubulin alpha in human A2780 cells assessed as cell growth inhibition after 48 hrs by MTT assay
[PMID: 30693770]
A2780 ADR IC50
0.003 μM
Compound: Pironetin
Cytotoxicity against compound-resistant human A2780AD cells after 24 hrs by MTT assay
Cytotoxicity against compound-resistant human A2780AD cells after 24 hrs by MTT assay
[PMID: 21396747]
A2780 ADR IC50
0.025 μM
Compound: pironetin
Cytotoxicity against human A2780AD cells after 24 hrs by MTT assay
Cytotoxicity against human A2780AD cells after 24 hrs by MTT assay
[PMID: 25426924]
A549 GI50
7.5 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against human A549 cells assessed as reduction in growth after 4 days by HCS assay
Cytotoxicity against human A549 cells assessed as reduction in growth after 4 days by HCS assay
[PMID: 31130264]
DC3F GI50
9.5 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against hamster DC-3F cells assessed as reduction in growth after 4 days by HCS assay
Cytotoxicity against hamster DC-3F cells assessed as reduction in growth after 4 days by HCS assay
[PMID: 31130264]
EL4 GI50
15 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against C57BL/6 mouse EL4 cells assessed as reduction in growth after 3 days
Cytotoxicity against C57BL/6 mouse EL4 cells assessed as reduction in growth after 3 days
[PMID: 31130264]
HCT-15 IC50
< 0.015 μM
Compound: Pironetin
Cytotoxicity against human HCT15 cells after 48 hrs by sulforhodamine B assay
Cytotoxicity against human HCT15 cells after 48 hrs by sulforhodamine B assay
[PMID: 30601004]
HEK293 IC50
0.017 μM
Compound: pironetin
Cytotoxicity against human HEK293 cells after 3 days by MTT assay
Cytotoxicity against human HEK293 cells after 3 days by MTT assay
[PMID: 25426924]
HEK293 IC50
0.017 μM
Compound: Pironetin
Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
[PMID: 25240870]
HeLa IC50
0.092 μM
Compound: Pironetin
Cytotoxicity against human HeLa cells after 48 hrs by sulforhodamine B assay
Cytotoxicity against human HeLa cells after 48 hrs by sulforhodamine B assay
[PMID: 30601004]
HL-60 GI50
20 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against human HL60 cells assessed as reduction in growth after 24 hrs by MTT assay
Cytotoxicity against human HL60 cells assessed as reduction in growth after 24 hrs by MTT assay
[PMID: 31130264]
HT-29 IC50
0.0064 μM
Compound: pironetin
Cytotoxicity against human HT-29 cells after 3 days by MTT assay
Cytotoxicity against human HT-29 cells after 3 days by MTT assay
[PMID: 25426924]
HT-29 IC50
0.0064 μM
Compound: Pironetin
Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
[PMID: 25240870]
K562 GI50
17.3 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against human K562 cells assessed as reduction in growth after 4 days by MTT assay
Cytotoxicity against human K562 cells assessed as reduction in growth after 4 days by MTT assay
[PMID: 31130264]
K562/Adr GI50
16 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against human K562/ADR cells assessed as reduction in growth after 4 days by MTT assay
Cytotoxicity against human K562/ADR cells assessed as reduction in growth after 4 days by MTT assay
[PMID: 31130264]
MCF7 IC50
0.006 μM
Compound: pironetin
Cytotoxicity against human MCF7 cells after 3 days by MTT assay
Cytotoxicity against human MCF7 cells after 3 days by MTT assay
[PMID: 25426924]
MCF7 IC50
0.006 μM
Compound: Pironetin
Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth after 72 hrs by MTT assay
[PMID: 25240870]
MCF7 IC50
0.015 μM
Compound: Pironetin
Cytotoxicity against human MCF7 cells after 48 hrs by sulforhodamine B assay
Cytotoxicity against human MCF7 cells after 48 hrs by sulforhodamine B assay
[PMID: 30601004]
MCF7 GI50
5 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against human MCF7 cells assessed as reduction in growth after 3 days by MTT assay
Cytotoxicity against human MCF7 cells assessed as reduction in growth after 3 days by MTT assay
[PMID: 31130264]
MDA-MB-231 GI50
4.6 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in growth after 4 days by HCS assay
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in growth after 4 days by HCS assay
[PMID: 31130264]
NCI-H69 GI50
17.5 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against human H69 cells assessed as reduction in growth after 7 days by MTT assay
Cytotoxicity against human H69 cells assessed as reduction in growth after 7 days by MTT assay
[PMID: 31130264]
P388 GI50
100 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against mouse P388 cells assessed as reduction in growth after 24 hrs by MTT assay
Cytotoxicity against mouse P388 cells assessed as reduction in growth after 24 hrs by MTT assay
[PMID: 31130264]
T98G GI50
7.5 nM
Compound: 1; (-)-PA-48153C
Cytotoxicity against human T98G cells assessed as reduction in growth after 4 days by MTS assay
Cytotoxicity against human T98G cells assessed as reduction in growth after 4 days by MTS assay
[PMID: 31130264]
體外研究
(In Vitro)

Pironetin (20-100 ng/mL; 24 hours; 3Y1 cells) treatment arrests the cell cycle progression at G2/M in 3Y1 cells[1].
Pironetin (1-10000 ng/mL; 3 days; HeLa, A2780 and K-NRK cells) treatment inhibits the cell proliferation. IC50 values against these cell lines are almost 10 ng/mL[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis[1]

Cell Line: 3Y1 cells
Concentration: 20 ng/mL, 50 ng/mL, 100 ng/mL
Incubation Time: 24 hours
Result: Arrested the cell cycle progression at G2/M in3Y1 cells.

Cell Proliferation Assay[1]

Cell Line: HeLa, A2780 and K-NRK cells
Concentration: 1 ng/mL, 10 ng/mL, 100 ng/mL, 1000 ng/mL and 10000 ng/mL
Incubation Time: 3 days
Result: Inhibited the cell proliferation.
體內(nèi)研究
(In Vivo)

Pironetin (0.78-6.25 mg/kg; intraperitoneal injection; daily; for 5 days; female CDF1-SLC mice) treatment shows a moderate antitumor effect, however, a severe weight loss is observed as a side effect[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female CDF1-SLC mice (10 weeks) injected with P388 murine leukemia cells[1]
Dosage: 0.78 mg/kg, 1.56 mg/kg, 3.13 mg/kg, 6.25 mg/kg
Administration: Intraperitoneal injection; daily; for 5 days
Result: Showed a moderate antitumor effect.
分子量

324.45

Formula

C19H32O4
CAS 號
性狀

油狀物

顏色

Colorless to off-white

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性數(shù)據(jù)
細(xì)胞實(shí)驗： 

DMSO 中的溶解度 : < 1 mg/mL (insoluble or slightly soluble)

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實(shí)驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實(shí)驗過程有損耗，建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料
參考文獻(xiàn)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的產(chǎn)品:

Your information is safe with us. * Required Fields.

   產(chǎn)品名稱：

  

* 需求量：

* 客戶姓名：

 

* Email：

* 電話：

 

* 公司或機(jī)構(gòu)名稱：

   留言給我們：

Bulk Inquiry

Inquiry Information

產(chǎn)品名稱：
Pironetin
目錄號：
HY-116446
需求量：