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  4. 7β-Hydroxycholesterol

7β-Hydroxycholesterol 是一種通過膽固醇氧化得到的氧固醇。7β-Hydroxycholesterol 是一種強大的氧化應(yīng)激誘導(dǎo)劑，可誘導(dǎo)細(xì)胞器 (線粒體、溶酶體和過氧化物酶體) 功能障礙，從而導(dǎo)致細(xì)胞死亡。

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7β-Hydroxycholesterol Chemical Structure

7β-Hydroxycholesterol Chemical Structure

CAS No. : 566-27-8

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥1594
In-stock
1 mg ￥600
In-stock
5 mg ￥1800
In-stock
10 mg ￥2900
In-stock
50 mg   詢價  
100 mg   詢價  

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Customer Review

Other Forms of 7β-Hydroxycholesterol:

MCE 顧客使用本產(chǎn)品發(fā)表的 1 篇科研文獻(xiàn)

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

7β-Hydroxycholesterol is an oxysterol that derived by the oxidation of cholesterol. 7β-hydroxycholesterol is a powerful inducer of oxidative stress, inducing dysfunction of organelles (mitochondria, lysosomes and peroxisomes) that can cause cell death[1].

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
13.6 μM
Compound: 6
Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
A549 IC50
13.6 μM
Compound: 8
Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human A549 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
ARPE-19 IC50
21.6 μM
Compound: 6
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human ARPE19 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
ARPE-19 IC50
21.6 μM
Compound: 8
Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human ARPE19 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
BJ IC50
20 μM
Compound: 8
Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay
Cytotoxicity against human BJ cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
BJ IC50
20.1 μM
Compound: 6
Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human BJ cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
BJ IC50
20.1 μM
Compound: 3
Cytotoxicity against human BJ cells after 48 hrs by Alamar Blue assay
Cytotoxicity against human BJ cells after 48 hrs by Alamar Blue assay
[PMID: 19473028]
C6 IC50
15 μM
Compound: 7beta-OHC
Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method
Growth inhibition of rat C6 cells after 24 hrs by trypan blue-based cell counting method
[PMID: 24211631]
HEK293 EC50
10 μM
Compound: 5
Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis
Binding affinity to human GFP-tagged NPC1L1 L1072T/L1168I mutant expressed in HEK293 cells assessed as localization to endoplasmic reticulum and plasma membrane after 24 hrs by fluorescence microscopic analysis
[PMID: 23830695]
HEK293 EC50
3.4 μM
Compound: 10
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
Binding affinity to FLAG/tGFP-tagged NPC1 I1061T mutant (unknown origin) expressed in HEK293 cells assessed as localization after 24 hrs by fluorescence microscopy
[PMID: 24928400]
HepG2 IC50
14.6 μM
Compound: 6
Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human HepG2 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
HepG2 IC50
14.6 μM
Compound: 8
Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human HepG2 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
HMEC-1 IC50
19.1 μM
Compound: 8
Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay
Cytotoxicity against HMEC1 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
HT-29 IC50
6.4 μM
Compound: 3
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay
[PMID: 19473028]
HT-29 IC50
6.9 μM
Compound: 6
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human HT-29 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
HT-29 IC50
6.9 μM
Compound: 8
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
HT-29 IC50
7.2 μM
Compound: 8
Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay
Cytotoxicity against human HT-29 cells after 96 hrs by Alamar blue assay
[PMID: 20931970]
LAMA-84 IC50
4.3 μM
Compound: 6
Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human LAMA-84 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
LAMA-84 IC50
4.3 μM
Compound: 8
Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
LAMA-84 IC50
4.8 μM
Compound: 3
Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar Blue assay
Cytotoxicity against human LAMA-84 cells after 48 hrs by Alamar Blue assay
[PMID: 19473028]
MCF7 IC50
8.7 μM
Compound: 6
Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
MCF7 IC50
8.7 μM
Compound: 8
Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human MCF7 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
PC-3 IC50
21.9 μM
Compound: 8
Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay
Cytotoxicity against human PC3 cells after 48 hrs by Alamar blue assay
[PMID: 20931970]
PC-3 IC50
22 μM
Compound: 6
Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human PC3 cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
SH-SY5Y IC50
16.4 μM
Compound: 6
Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay
Cytotoxicity against human SH-SY5Y cells assessed as cell viability after 48 hrs by alamar blue assay
[PMID: 21797280]
分子量

402.65

Formula

C27H46O2
CAS 號
性狀

固體

顏色

White to off-white

運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細(xì)胞實驗： 

DMSO 中的溶解度 : 100 mg/mL (248.35 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.21 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.21 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.98%

參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4835 mL 12.4177 mL 24.8355 mL 62.0887 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL 12.4177 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL 6.2089 mL
15 mM 0.1656 mL 0.8278 mL 1.6557 mL 4.1392 mL
20 mM 0.1242 mL 0.6209 mL 1.2418 mL 3.1044 mL
25 mM 0.0993 mL 0.4967 mL 0.9934 mL 2.4835 mL
30 mM 0.0828 mL 0.4139 mL 0.8278 mL 2.0696 mL
40 mM 0.0621 mL 0.3104 mL 0.6209 mL 1.5522 mL
50 mM 0.0497 mL 0.2484 mL 0.4967 mL 1.2418 mL
60 mM 0.0414 mL 0.2070 mL 0.4139 mL 1.0348 mL
80 mM 0.0310 mL 0.1552 mL 0.3104 mL 0.7761 mL
100 mM 0.0248 mL 0.1242 mL 0.2484 mL 0.6209 mL
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產(chǎn)品名稱：
7β-Hydroxycholesterol
目錄號：
HY-113341
需求量：