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  2. Cell Cycle/DNA Damage Anti-infection
  3. Nucleoside Antimetabolite/Analog HIV
  4. Pseudothymidine

Pseudothymidine  (Synonyms: 5-Methyl-2'-Deoxypseudouridin)

目錄號: HY-101969 純度: 99.44%
COA 產(chǎn)品使用指南

Pseudothymidine 是胸苷的 C-核苷 (C-nucleosid) 類似物。

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Pseudothymidine Chemical Structure

Pseudothymidine Chemical Structure

CAS No. : 65358-15-8

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥5577
In-stock
1 mg ￥950
In-stock
5 mg ￥2170
In-stock
10 mg ￥3620
In-stock
25 mg 現(xiàn)貨 詢價
50 mg   詢價  
100 mg   詢價  

* Please select Quantity before adding items.

Customer Review

查看 HIV 亞型特異性產(chǎn)品:

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Pseudothymidine is a C-nucleoside analog of thymidine.

體外研究
(In Vitro)

Pseudothymidine is a C-nucleoside analog of thymidine[1]. The calculated ΔΔG°50/mod is -0.5 kcal/mol, with a ΔTm/mod of 0.82°C. For the duplexes containing nine dA-T/ψT pairs, the ΔTm/mod is -0.9°C and a ΔΔG°50/mod is +1.1 kcal/mol. The modification of the duplex containing 12 consecutive dA-T/ψT base pairs produces a ΔTm/mod of -0.9°C and a ΔΔG°50/mod of +1.2 kcal/mol[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

242.23

Formula

C10H14N2O5
CAS 號
性狀

固體

顏色

Light yellow to yellow

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性數(shù)據(jù)
細(xì)胞實驗： 

DMSO 中的溶解度 : 61.17 mg/mL (252.53 mM; 超聲加熱助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 4.1283 mL 20.6415 mL 41.2831 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料
參考文獻(xiàn)
Kinase Assay

Thermal DNA duplex denaturation studies are performed with templates containing up to twelve consecutive dA residues that are paired with its complement template containing consecutive T or Pseudothymidine (ψT) residues. Experiments are performed in a buffer (45 mM NaCl, 45 mM sodium citrate, pH 8.1, final vol. 1.5 mL) containing template and its complement (1.5 μM of each). Absorbance (260 nm) is monitored over a range of 25.0 to 90.0°C with a change in temperature of 0.5°C/min for five heating cycles. The initial heating cycle is discarded and the Tm is determined by averaging the temperatures of the remaining four cycles. The ΔTm between similar duplexes is calculated by subtracting the Tm of the duplex containing standard bases from the Tm of the duplex containing C-glycosides (including Pseudothymidine)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1283 mL 20.6415 mL 41.2831 mL 103.2077 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL 20.6415 mL
10 mM 0.4128 mL 2.0642 mL 4.1283 mL 10.3208 mL
15 mM 0.2752 mL 1.3761 mL 2.7522 mL 6.8805 mL
20 mM 0.2064 mL 1.0321 mL 2.0642 mL 5.1604 mL
25 mM 0.1651 mL 0.8257 mL 1.6513 mL 4.1283 mL
30 mM 0.1376 mL 0.6881 mL 1.3761 mL 3.4403 mL
40 mM 0.1032 mL 0.5160 mL 1.0321 mL 2.5802 mL
50 mM 0.0826 mL 0.4128 mL 0.8257 mL 2.0642 mL
60 mM 0.0688 mL 0.3440 mL 0.6881 mL 1.7201 mL
80 mM 0.0516 mL 0.2580 mL 0.5160 mL 1.2901 mL
100 mM 0.0413 mL 0.2064 mL 0.4128 mL 1.0321 mL
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產(chǎn)品名稱：
Pseudothymidine
目錄號：
HY-101969
需求量：