CU-CPT17e

目錄號: HY-101929 純度: 99.00%: FAQs
Data Sheet SDS COA 產(chǎn)品使用指南

摩爾計算器

CU-CPT17e 是一種有效的多 Toll 樣受體 (TLR) 激動劑，可激活 TLR3，TLR8 和 TLR9。

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CU-CPT17e Chemical Structure

CAS No. : 2109805-75-4

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1 mg	￥2100	In-stock
5 mg	￥4400	In-stock
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Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 3 篇科研文獻(xiàn)

•Wound Repair Regen. 2022 May;30(3):376-396. [Abstract]
•Research Square Preprint. 2022 Jul.
•Research Square Preprint. 2021 Mar.

CU-CPT17e 相關(guān)產(chǎn)品

•相關(guān)化合物庫:

Bioactive Compound Library Plus

•同靶點(diǎn)產(chǎn)品:

•同靶點(diǎn)蛋白產(chǎn)品:

查看 Toll-like Receptor (TLR) 亞型特異性產(chǎn)品:

查看所有亞型

TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

生物活性
實驗參考方法
純度 & 產(chǎn)品資料
參考文獻(xiàn)

生物活性

CU-CPT17e is a potent multi-Toll-like receptor (TLR) agonist that activates TLR3, TLR8, and TLR9.

IC₅₀ & Target^[1]

TLR3

TLR8

TLR9

細(xì)胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR2 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR2 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR4 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR4 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR5 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR7 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	13.45 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR8 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	4.8 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR3 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR3 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HEK293	EC₅₀	5.66 μM Compound: 17e; CU-CPT17e	Agonist activity at human TLR9 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay Agonist activity at human TLR9 expressed in HEK293 cells assessed as induction of NF-kB activation-mediated SEAP production after 24 hrs by HTS assay	[PMID: 28537730]
HeLa	IC₅₀	15.77 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against human HeLa cells after 48 hrs by WST1 assay Antiproliferative activity against human HeLa cells after 48 hrs by WST1 assay	[PMID: 28537730]
HeLa	IC₅₀	2.71 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against human HeLa cells after 72 hrs by WST1 assay Antiproliferative activity against human HeLa cells after 72 hrs by WST1 assay	[PMID: 28537730]
HeLa	IC₅₀	38.56 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against human HeLa cells after 24 hrs by WST1 assay Antiproliferative activity against human HeLa cells after 24 hrs by WST1 assay	[PMID: 28537730]
HMEC	IC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against HMEC after 24 hrs by WST1 assay Antiproliferative activity against HMEC after 24 hrs by WST1 assay	[PMID: 28537730]
HMEC	IC₅₀	> 100 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against HMEC after 48 hrs by WST1 assay Antiproliferative activity against HMEC after 48 hrs by WST1 assay	[PMID: 28537730]
HMEC	IC₅₀	79.53 μM Compound: 17e; CU-CPT17e	Antiproliferative activity against HMEC after 72 hrs by WST1 assay Antiproliferative activity against HMEC after 72 hrs by WST1 assay	[PMID: 28537730]

體外研究
(In Vitro)

CU-CPT17e 在 TLR3、TLR8 和 TLR9 HEK293 細(xì)胞中表現(xiàn)出強(qiáng)烈的 NF-κB 激活，EC₅₀ 值分別為 4.80±0.73、13.5±0.58 和 5.66±0.17 μM。CU-CPT17e 顯著提高活性，NF-κB 激活增加 13.9±0.9 倍，EC₅₀ 值為 4.8±0.7 μM。CU-CPT17e 通過觸發(fā)細(xì)胞凋亡和將細(xì)胞周期停滯在 S 期來抑制 HeLa 癌細(xì)胞的增殖。研究了 CU-CPT17e 在 HeLa 細(xì)胞中誘導(dǎo)細(xì)胞凋亡。HeLa 細(xì)胞在濃度不斷增加的 CU-CPT17e 或聚 I:C 或空白對照 (DMSO) 中培養(yǎng) 24 小時。用不同濃度 (10 至 40 μM) 的 CU-CPT17e 處理 24 小時可導(dǎo)致凋亡細(xì)胞群升高 10% 至 17%，這比 5 μg/mL 的 poly I:C 更有效。這些結(jié)果表明，CU-CPT17e 對 HeLa 細(xì)胞的抗增殖活性可能與其直接誘導(dǎo)細(xì)胞凋亡的能力有關(guān)^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

504.49

Formula

C₂₇H₂₄N₂O₈

CAS 號

2109805-75-4

性狀

固體

顏色

Light yellow to yellow

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性數(shù)據(jù)

細(xì)胞實驗：

DMSO 中的溶解度 : 5 mg/mL (9.91 mM; 超聲加熱助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	1.9822 mL	9.9110 mL	19.8220 mL
5 mM	0.3964 mL	1.9822 mL	3.9644 mL
10 mM	---	---	---

查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

摩爾計算器
稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動物溶解方案計算器

請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

每只動物的給藥體積

μL

動物數(shù)量

只

由于實驗過程有損耗，建議您多配一只動物的量

計算結(jié)果

工作液所需濃度 : mg/mL

純度 & 產(chǎn)品資料

純度: 99.00%

選擇批次：

Data Sheet (620 KB) SDS (544 KB)

COA (288 KB) HNMR (270 KB) RP-HPLC (198 KB) MS (69 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻(xiàn)

[1]. Zhang L, et al. Discovery of Small Molecules as Multi-Toll-like Receptor Agonists with Proinflammatory and Anticancer Activities. J Med Chem. 2017 Jun 22;60(12):5029-5044. [Content Brief]

Cell Assay ^[1]	HeLa cells are seeded at a density of 3×10⁵ cells/well in 6-well plates and allowed to attach for 24 h. After treatment of indicated concentrations of CU-CPT17e or poly I:C (5 μg/mL) for another 24 h, cells are harvested with 0.25% trypsin without EDTA and rinsed twice with PBS, then stained using a Annexin V-FITC apoptosis detection kit. Cells are analyzed with a BD Accuri C6 flow cytometer^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)	[1]. Zhang L, et al. Discovery of Small Molecules as Multi-Toll-like Receptor Agonists with Proinflammatory and Anticancer Activities. J Med Chem. 2017 Jun 22;60(12):5029-5044. [Content Brief]

Cell Assay
^[1]

HeLa cells are seeded at a density of 3×10⁵ cells/well in 6-well plates and allowed to attach for 24 h. After treatment of indicated concentrations of CU-CPT17e or poly I:C (5 μg/mL) for another 24 h, cells are harvested with 0.25% trypsin without EDTA and rinsed twice with PBS, then stained using a Annexin V-FITC apoptosis detection kit. Cells are analyzed with a BD Accuri C6 flow cytometer^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

參考文獻(xiàn)

[1]. Zhang L, et al. Discovery of Small Molecules as Multi-Toll-like Receptor Agonists with Proinflammatory and Anticancer Activities. J Med Chem. 2017 Jun 22;60(12):5029-5044. [Content Brief]

CU-CPT17e 相關(guān)分類

完整儲備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.9822 mL	9.9110 mL	19.8220 mL	49.5550 mL
DMSO	5 mM	0.3964 mL	1.9822 mL	3.9644 mL	9.9110 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CU-CPT17e2109805-75-4Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

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CU-CPT17e

Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 3 篇科研文獻(xiàn)