成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

  2. Others
  3. Fluorescent Dye
  4. Fluo-4 AM

Fluo-4 AM 是一種常用的檢測細胞內(nèi) Ca2+ (鈣離子) 濃度的探針。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務

Fluo-4 AM Chemical Structure

Fluo-4 AM Chemical Structure

CAS No. : 273221-67-3

1.  客戶無需承擔相應的運輸費用。

2.  同一機構(gòu)（單位）同一產(chǎn)品試用裝僅限申領一次，同一機構(gòu)（單位）一年內(nèi)

     可免費申領三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
100 μg ￥1500
In-stock

* Please select Quantity before adding items.

Customer Review

    Fluo-4 AM purchased from MCE. Usage Cited in: Front Pharmacol. 2022 May 17;13:855626.  [Abstract]

    Intracellular Ca2+ is measured by Fluo-4/AM (for 30 min) with flow cytometry.

    Fluo-4 AM purchased from MCE. Usage Cited in: Cell Death Discov. 2021 Feb 10;7(1):31.  [Abstract]

    The cardiomyocytes are isolated by enzyme digestion and loaded with Fluo 4-AM (5?μM) at 37?°C for 20?min. Fluorescence changes of Fluo 4-AM are recorded by confocal microscopy at 25?°C.

    Fluo-4 AM purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2021 Feb 10;2021:8884922.

    NP cells are incubated with 5?μM Fura-4-AM for 30?min at 37°C in the dark. Intracellular Ca2+ levels measured using the specific Ca2+-sensitive fluorescent indicator Fura-4-AM and analyzed via fluorescence microscopy.

    • 生物活性

    • 實驗參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Fluo-4 AM is a cell-permeable Ca2+ (calcium ion) indicator[1].

    體外研究
    (In Vitro)

    Fluo-4 AM 是一種熒光染料 (λex=494 nm，λem=516 nm)。預裝 Fuo-4 AM，可以觀察到非常明亮的熒光圖像。在使用 fluo-3 AM 加載細胞進行的平行實驗中，得到的熒光圖像雖然在這種情況下清晰可辨，但不太亮[1]。
    指南 (以下是我們推薦的方案。本方案僅提供指南，應根據(jù)您的具體需要進行修改)。
    1. 計數(shù)細胞，從每個樣本 (對照和實驗) 中取106個細胞。
    2. 收集細胞 (5分鐘，3000×g，4℃)，用PBS洗滌一次。
    3. 用 0.5-ml PBS重懸細胞，加入 0.5 ul Fluo-4- AM (1 mM 庫存) 至最終濃度為 1 μM。37℃ 孵育 1 小時。
    4. 用 PBS 洗滌細胞 3 次 (2分鐘，3000×g)，最后用 1 ml PBS重懸。分成 2 個流式細胞儀管，每管 0.5 ml。
    5. 用流式細胞術評估染色，并用 CellQuest 軟件等軟件分析數(shù)據(jù)。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    1096.94

    Formula

    C51H50F2N2O23
    CAS 號
    性狀

    固體

    顏色

    Brown to red

    Emission (Em)

    526
    Excitation (Ex)

    485
    運輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    -20°C, sealed storage, away from moisture and light

    *該產(chǎn)品在溶液狀態(tài)不穩(wěn)定，建議您現(xiàn)用現(xiàn)配，即刻使用。

    純度 & 產(chǎn)品資料

    純度: ≥99.0%

    染色示例
    參考文獻
    Cell Assay
    [1]

    For measuring fluorescence from cells in suspension, dilutions to 2 to 3×106 cells are made, from cultures of rat basophilic leukemia (RBL) cells. Cells are incubated in suspension in 1 μM dye (including Fluo-4 AM) for 30 min at 37°C. Cell suspensions are then transferred to cuvets for measurements of fluorescence emission intensity by spectrofluorometer[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻

    • 摩爾計算器

    • 稀釋計算器

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量   濃度   體積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    您最近查看的產(chǎn)品:

    Your information is safe with us. * Required Fields.

       產(chǎn)品名稱：

    		  

    * 需求量：

    * 客戶姓名：

    		 

    * Email：

    * 電話：

    		 

    * 公司或機構(gòu)名稱：

       留言給我們：

    Bulk Inquiry

    Inquiry Information

    產(chǎn)品名稱：
    Fluo-4 AM
    目錄號：
    HY-101896
    需求量：