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  4. 7-Methoxycoumarin-3-carboxylic acid

7-Methoxycoumarin-3-carboxylic acid 

目錄號(hào): HY-W039519 純度: ≥97.0%
COA 產(chǎn)品使用指南 技術(shù)支持

7-Methoxycoumarin-3-carboxylic acid 是一種熒光染料，其激發(fā)峰位于 355 nm，發(fā)射峰位于 405 nm。7-Methoxycoumarin-3-carboxylic acid可用于標(biāo)記肽類。

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7-Methoxycoumarin-3-carboxylic acid Chemical Structure

7-Methoxycoumarin-3-carboxylic acid Chemical Structure

CAS No. : 20300-59-8

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規(guī)格 價(jià)格 是否有貨 數(shù)量
25 mg ￥480
In-stock
50 mg ￥820
In-stock
100 mg ￥1400
In-stock
1 g ￥3500
詢價(jià)
5 g   詢價(jià)  
10 g   詢價(jià)  

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Customer Review

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

7-Methoxycoumarin-3-carboxylic acid is a fluorescent dye with an excitation peak at 355 nm and an emission peak at 405 nm. 7-Methoxycoumarin-3-carboxylic acid can be used to label peptide[1].

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
Oocyte IC50
168 μM
Compound: 7-OMe-Coum-COOH
Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique
Antagonist activity at Gloeobacter violaceus ligand-gated ion channel expressed in Xenopus oocytes assessed as inhibition of MES buffer pH 5.5 -induced currents after 30 secs by voltage clamp technique
[PMID: 23682762]
SiHa IC50
2.3 μM
Compound: 1
Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting
Inhibition of MCT4-mediated [14C]-lactate uptake in human SiHa cells in lactate-containing medium assessed as remaining lactate concentration in culture medium after 24 hrs by liquid scintillation counting
[PMID: 24095010]
SiHa EC50
9.8 μM
Compound: 1
Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay
Cytotoxicity against human SiHa cells in lactate-containing medium after 72 hrs by MTT assay
[PMID: 24095010]
體外研究
(In Vitro)

7-Methoxycoumarin-3-carboxylic acid (compound 5) (100 μM; 72 h) shows non-toxic for K562, Daudi, RPMI-8226, and Jurkat cells with IC50s of >100, >100, >100, >100 μM, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

220.18

Formula

C11H8O5
CAS 號(hào)
性狀

固體

顏色

White to off-white

Emission (Em)

450
Excitation (Ex)

345
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 8 mg/mL (36.33 mM; 超聲助溶 (<60°C); 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 4.5417 mL 22.7087 mL 45.4174 mL
5 mM 0.9083 mL 4.5417 mL 9.0835 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料
參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5417 mL 22.7087 mL 45.4174 mL 113.5435 mL
5 mM 0.9083 mL 4.5417 mL 9.0835 mL 22.7087 mL
10 mM 0.4542 mL 2.2709 mL 4.5417 mL 11.3543 mL
15 mM 0.3028 mL 1.5139 mL 3.0278 mL 7.5696 mL
20 mM 0.2271 mL 1.1354 mL 2.2709 mL 5.6772 mL
25 mM 0.1817 mL 0.9083 mL 1.8167 mL 4.5417 mL
30 mM 0.1514 mL 0.7570 mL 1.5139 mL 3.7848 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
7-Methoxycoumarin-3-carboxylic acid
目錄號(hào)：
HY-W039519
需求量：