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  2. PI3K/Akt/mTOR Apoptosis
  3. Akt mTOR Caspase
  4. C-Reactive Protein (CRP) (174-185)

C-Reactive Protein (CRP) (174-185) 

目錄號: HY-P1823 純度: 99.83%
COA 產(chǎn)品使用指南 技術(shù)支持

C-Reactive Protein (CRP) 是一種抗肺炎球菌血漿蛋白，可作為炎癥標(biāo)志物。C-Reactive Protein 可以通過激活補(bǔ)體保護(hù)小鼠免受肺炎球菌感染。C-Reactive Protein 可以通過 CD64/AKT/mTOR 通路抑制 caspase-3/9 的激活從而促進(jìn)舌鱗狀細(xì)胞癌小鼠的化療耐藥性。

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Custom Peptide Synthesis

C-Reactive Protein (CRP) (174-185) Chemical Structure

C-Reactive Protein (CRP) (174-185) Chemical Structure

CAS No. : 147516-85-6

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規(guī)格 價格 是否有貨 數(shù)量
1 mg ￥750
In-stock
5 mg ￥1850
In-stock
10 mg ￥2950
In-stock
50 mg   詢價  
100 mg   詢價  

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Customer Review

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

C-Reactive Protein (CRP) is an anti-pneumococcal plasma protein that can serve as an inflammatory marker. C-Reactive protein can protect mice from pneumococcal infection by activating complement. C-Reactive protein can inhibit the activation of caspase-3/9 through the CD64/AKT/mTOR pathway, thereby promoting chemotherapy resistance in mice with tongue squamous cell carcinoma[1][5].
體外研究
(In Vitro)

C-Reactive Protein (CRP) (0-25 μg/mL; 24 h) 通過刺激 3T3-L1 脂肪細(xì)胞產(chǎn)生 CXCL7 來促進(jìn)單核細(xì)胞遷移[3]。
C-Reactive Protein (CRP) 可以損害 G1/S 依賴性腎小管上皮細(xì)胞再生[4]。
C-Reactive Protein (CRP) (10 μg/mL, 4 天) 可以提高 TSCC 細(xì)胞活力并增加細(xì)胞對 DDP 的耐藥性[5]。
C-Reactive Protein (CRP) 是一種在結(jié)構(gòu)上高度保守的人類主要急性期血漿蛋白[6]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Migration Assay [3].

Cell Line: 3T3-L1 adipocytes
Concentration: 0-25 μg/mL
Incubation Time: 24 h
Result: Promoted cell migration.

Cell Viability Assay[5].

Cell Line: The human TSCC cell lines CAL27 and SCC4
Concentration: 10 μg/mL
Incubation Time: 4 days
Result: Improved TSCC cell viability.
體內(nèi)研究
(In Vivo)

C-Reactive Protein (CRP) (25 μg; 每天 1 次; 7 天; 靜脈注射) 可以通過激活補(bǔ)體保護(hù)小鼠免受肺炎球菌感染[1]。
C-Reactive Protein (CRP) (20 mg/kg; 每天 1 次; 3 天; 腹腔注射) 可以誘導(dǎo)大鼠巨噬細(xì)胞中的超氧陰離子釋放和組織因子活性[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: A murine model of pneumococcal infection[1].
Dosage: 25 μg
Administration: Intravenous injection (i.v.); once daily; 7 days
Result: Activated complement in mouse serum.
Animal Model: Male hooded Wistar rats (weighing 125-150 g)[2].
Dosage: 20 mg/kg
Administration: Intraperitoneal injection (i.p.); once daily; 3 days
Result: Upregulated PKC, NADPH oxidase, ERK, and JNK phosphorylation.
分子量

1276.48

Formula

C62H93N13O16
CAS 號
性狀

固體

顏色

White to off-white

Sequence

Ile-Tyr-Leu-Gly-Gly-Pro-Phe-Ser-Pro-Asn-Val-Leu
Sequence Shortening

IYLGGPFSPNVL
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
細(xì)胞實驗： 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
純度 & 產(chǎn)品資料
參考文獻(xiàn)

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量   濃度   體積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start) × 體積 (start) = 濃度 (final) × 體積 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
C-Reactive Protein (CRP) (174-185)
目錄號：
HY-P1823
需求量：