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  1. 多肽產(chǎn)品
  2. 多肽及其衍生物
  3. 肽表位
  4. OVA Peptide(257-264)

OVA Peptide(257-264) 是 OVA 的 I 類 (Kb)-限制性肽表位,它是一種八聚體肽,可以來自 I 類 MHC 分子 H-2Kb 所呈現(xiàn)的卵清蛋白。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報,我們不為任何個人用途提供產(chǎn)品和服務

Custom Peptide Synthesis

OVA Peptide(257-264) Chemical Structure

OVA Peptide(257-264) Chemical Structure

CAS No. : 138831-86-4

1.  客戶無需承擔相應的運輸費用。

2.  同一機構(gòu)(單位)同一產(chǎn)品試用裝僅限申領(lǐng)一次,同一機構(gòu)(單位)一年內(nèi)

     可免費申領(lǐng)三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶

規(guī)格 價格 是否有貨 數(shù)量
1 mg ¥720
In-stock
5 mg ¥1360
In-stock
10 mg ¥2160
In-stock
50 mg   詢價  
100 mg   詢價  

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Customer Review

Other Forms of OVA Peptide(257-264):

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

OVA Peptide(257-264) is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide can be from ovalbumin presented by the class I MHC molecule, H-2Kb.

體外研究
(In Vitro)

TAP1-I- and C57BL/6 macrophages may process Crl-OVA and full-length OVA in different cellular compartments and that the protein context of the OVA Peptide(257-264) epitope influences the extent of TAP-independent processing for MHC class I presentation. OVA Peptide(257-264) epitope is presented with a differential dependence on the TAP transporter depending on the protein context of the OVA epitope: OVA Peptide(257-264) contained within the MBPCrl-OVA or Crl-OVA bacterial fusion proteins is presented with little dependence on the TAP transporter, while OVA Peptide(257-264) contained within full-length ovalbumin is largely dependent on the TAP transporter, regardless of whether recombinant OVA is expressed in bacteria or the native protein is coupled to polystyrene beads[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

963.13

Formula

C45H74N10O13

CAS 號
性狀

固體

顏色

White to off-white

Sequence

Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu

Sequence Shortening

SIINFEKL

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性數(shù)據(jù)
細胞實驗: 

DMSO 中的溶解度 : 40 mg/mL (41.53 mM; ultrasonic and adjust pH to 3 with 1 M HCL; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響,請使用新開封的 DMSO)

0.1% TFA in Water 中的溶解度 : 1.83 mg/mL (1.90 mM; 超聲助溶)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 1.0383 mL 5.1914 mL 10.3828 mL
5 mM 0.2077 mL 1.0383 mL 2.0766 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時,請在6個月內(nèi)使用,-20°C儲存時,請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物溶解方案計算器
請輸入動物實驗的基本信息:

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

由于實驗過程有損耗,建議您多配一只動物的量
計算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料

純度: 99.68%

參考文獻
Cell Assay

TAPl-/- or C57BL/6 macrophages are co-incubated with either bacteria or polystyrene beads containing the 257-264 epitope from ovalbumin [OVA Peptide(257-264)], which binds the mouse class I molecule Kb. The source of the OVA(257-264) epitope is either the Crl-OVA(257-264) (Crl-OVA) fusion protein, the maltose binding protein (MBP)-Crl-OVA fusion protein, native OVA or bacterial recombinant OVA (rOVA); Crl-OVA, MBP-Crl-OVA and rOVA are each expressed in bacteria, and Crl-OVA and MBP-Crl-OVA purified from bacterial lysates and native egg OVA are coated onto polystyrene beads[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產(chǎn)品失效
儲備液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時,請在6個月內(nèi)使用,-20°C儲存時,請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
0.1% TFA in Water / DMSO 1 mM 1.0383 mL 5.1914 mL 10.3828 mL 25.9570 mL
DMSO 5 mM 0.2077 mL 1.0383 mL 2.0766 mL 5.1914 mL
10 mM 0.1038 mL 0.5191 mL 1.0383 mL 2.5957 mL
15 mM 0.0692 mL 0.3461 mL 0.6922 mL 1.7305 mL
20 mM 0.0519 mL 0.2596 mL 0.5191 mL 1.2979 mL
25 mM 0.0415 mL 0.2077 mL 0.4153 mL 1.0383 mL
30 mM 0.0346 mL 0.1730 mL 0.3461 mL 0.8652 mL
40 mM 0.0260 mL 0.1298 mL 0.2596 mL 0.6489 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

產(chǎn)品名稱:
OVA Peptide(257-264)
目錄號:
HY-P1489
需求量: