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  2. Apoptosis
  3. Caspase
  4. Ac-DEVD-CHO

Ac-DEVD-CHO 是一種特異性 Caspase-3 抑制劑，Ki 值為 230 pM。

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Custom Peptide Synthesis

Ac-DEVD-CHO Chemical Structure

Ac-DEVD-CHO Chemical Structure

CAS No. : 169332-60-9

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10 mg ￥3500
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Customer Review

MCE 顧客使用本產(chǎn)品發(fā)表的 33 篇科研文獻(xiàn)

WB

    Ac-DEVD-CHO purchased from MCE. Usage Cited in: Apoptosis. 2019 Oct;24(9-10):703-717.  [Abstract]

    BMDMs were first primed with LPS (500 ng/ml) for 4 h, pre-treated with or without MCC950 (1 μM) and caspase-3 inhibitor Ac-DEVD-CHO (DEVD) for 1 h and followed by stimulation with ATP (5 mM) in the presence or absence of MCC950. The cell lysates are collected and western blotting is performed to assess the expression levels of indicated proteins.

    • 生物活性

    • 實(shí)驗(yàn)參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Ac-DEVD-CHO is a specific Caspase-3 inhibitor with a Ki value of 230 pM.

    IC50 & Target[1]

    Caspase 3

    0.23 nM (Ki)

    Caspase-8

    0.92 nM (Ki)

    Caspase-7

    1.6 nM (Ki)

    Caspase-10

    12 nM (Ki)

    Caspase-1

    18 nM (Ki)

    Caspase-6

    31 nM (Ki)

    Caspase-9

    60 nM (Ki)

    Caspase-4

    132 nM (Ki)

    Caspase-5

    205 nM (Ki)

    Caspase-2

    1710 nM (Ki)

    細(xì)胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    NT2 EC50
    > 100 μM
    Compound: Ac-DEVD-CHO
    Inhibition of camptothecin induced apoptosis in human NT2 cells incubated for 5 hrs in presence of camptothecin by ELISA method
    Inhibition of camptothecin induced apoptosis in human NT2 cells incubated for 5 hrs in presence of camptothecin by ELISA method
    		[PMID: 33569941]
    THP-1 IC50
    7.0 x 10-5 M
    Compound: 2a
    Amidolytic activity of THP.1 cell lysate against IL-1 beta converting enzyme using C1-Asp-AMC as a substrate
    Amidolytic activity of THP.1 cell lysate against IL-1 beta converting enzyme using C1-Asp-AMC as a substrate
    		[PMID: 9873373]
    體外研究
    (In Vitro)

    為了確定 caspase-3 在 SLNT 誘導(dǎo)的細(xì)胞凋亡中的作用，使用了 caspase-3 抑制劑 (Ac-DEVD-CHO)。添加Ac-DEVD-CHO顯著阻止SLNT誘導(dǎo)的細(xì)胞凋亡 (從32.91±1.21%下降到15.88±1.58%，而NC和Ac-DEVD-CHO組分別為6.45±0.96%、7.77±0.79%)[2]。
    zVAD-fmk (5.32%) 或Ac-DEVD-CHO (7.43%) 預(yù)處理的細(xì)胞凋亡率在金絲桃素介導(dǎo)的PDT處理后明顯下降[3]。
    值得注意的是，10 μMol/L Ac-DEVD-CHO 部分阻斷了 SIN 誘導(dǎo)的細(xì)胞凋亡的作用并減少了凋亡細(xì)胞核的數(shù)量。SIN 的這些作用被 caspase-3 抑制劑 Ac-DEVD-CHO 阻斷。陽性對照喜樹堿 (4 μM) 可增加 caspase-3 活性，該活性也被 Ac-DEVD-CHO 阻斷[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    體內(nèi)研究
    (In Vivo)

    與模型組比較，CI 組術(shù)后各時(shí)間點(diǎn)血清 BUN 濃度均顯著降低，Cr 濃度在術(shù)后 6 h 顯著降低，12 h、24 h 恢復(fù)至假手術(shù)組；血清 TNF-α、IL-6 濃度降低，IL-10 濃度在所有時(shí)間點(diǎn)均顯著升高。[TNF-α (ug/L) 6小時(shí)：436.2±64.2 vs. 653.6±8.9，12小時(shí)：233.4±85.4 vs. 579.7±137.1，24小時(shí)：151.0±90.3 vs. 551.0±119.8，IL-6 (ug/L) 6小時(shí)：1033.2±345.8 vs. 1 595.3±159.4，12小時(shí)：366.3±68.3 vs. 1 330.7±249.8，24小時(shí)：241.2±208.4 vs. 815.3±572.7，IL-10 (ug/L) 6小時(shí)：33.6±10.4 vs. 26.6±4.5，12小時(shí)：37.2±5.0 vs. 24.5±4.3，24小時(shí)：38.3±5.5 vs. 18.2±1.6，均P<0.05]；腎細(xì)胞凋亡率在各時(shí)間點(diǎn)均顯著下降：凋亡率6小時(shí)：(13.9±3.2) % vs. (18.3±1.4) %，12小時(shí)：(10.5±3.6) % vs. (15.9±3.5) %，24小時(shí)：(8.4±1.8) % vs. (12.5±2.1) %[5]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    502.47

    Formula

    C20H30N4O11
    CAS 號
    性狀

    固體

    顏色

    White to off-white

    Sequence

    N-Acetyl-Asp-Glu-Val-Asp-al
    Sequence Shortening

    Ac-DEVD-al
    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.
    儲存方式

    Sealed storage, away from moisture and light, under nitrogen

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn)： 

    H2O 中的溶解度 : ≥ 50 mg/mL (99.51 mM)

    * "≥" means soluble, but saturation unknown.

    配制儲備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 1.9902 mL 9.9508 mL 19.9017 mL
    5 mM 0.3980 mL 1.9902 mL 3.9803 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實(shí)驗(yàn)：

    以下溶解方案，請直接配制工作液。建議現(xiàn)用現(xiàn)配，在短期內(nèi)盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比； 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請依序添加每種溶劑： PBS

      Solubility: 100 mg/mL (199.02 mM); 澄清溶液; 超聲助溶

    動物溶解方案計(jì)算器
    請輸入動物實(shí)驗(yàn)的基本信息：

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    只
    由于實(shí)驗(yàn)過程有損耗，建議您多配一只動物的量
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    該產(chǎn)品水溶性佳，請具體參考實(shí)測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
    您所需的儲備液濃度超過該產(chǎn)品的實(shí)測溶解度，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
    純度 & 產(chǎn)品資料

    純度: 99.55%

    參考文獻(xiàn)
    Cell Assay
    [4]

    OCLs are incubated with RANKL and treated with 0.5 mM SIN with or without the specific caspase-3 inhibitor Ac-DEVD-CHO (10 μM) for 24 h. At the end of the treatment, the cells are washed with PBS and are stained for 15 min with 10 μM Hoechst 33258 dye. Images of the staineing cells are captured with a fluorescent microscope. The differences are evaluated by counting the number of cells with apoptotic nuclear condensation in each well[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [5]

    One hundred and two male mice are subjected to cecal ligation and puncture or sham operation. The animals are assigned into three equal groups (n=34) according to random number table: sham group, model group, and caspase-3 inhibitor (CI) group. Thirty minutes before CLP, Ac-DEVD-CHO (4 μg/g) is injected subcutaneously in CI group. The levels of blood urea nitrogen (BUN) and creatinine (Cr) are determined, and the concentrations of tumor necrosis factor-α (TNF-α), interleukins (IL-6 and IL-10) are measured by enzyme linked immunosorbent assay (ELISA), the renal cell apoptosis rate is determined by flow cytometry. The 4-day and 7-day survival rates of three groups of mice are observed[5].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    參考文獻(xiàn)

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.9902 mL 9.9508 mL 19.9017 mL 49.7542 mL
    5 mM 0.3980 mL 1.9902 mL 3.9803 mL 9.9508 mL
    10 mM 0.1990 mL 0.9951 mL 1.9902 mL 4.9754 mL
    15 mM 0.1327 mL 0.6634 mL 1.3268 mL 3.3169 mL
    20 mM 0.0995 mL 0.4975 mL 0.9951 mL 2.4877 mL
    25 mM 0.0796 mL 0.3980 mL 0.7961 mL 1.9902 mL
    30 mM 0.0663 mL 0.3317 mL 0.6634 mL 1.6585 mL
    40 mM 0.0498 mL 0.2488 mL 0.4975 mL 1.2439 mL
    50 mM 0.0398 mL 0.1990 mL 0.3980 mL 0.9951 mL
    60 mM 0.0332 mL 0.1658 mL 0.3317 mL 0.8292 mL
    80 mM 0.0249 mL 0.1244 mL 0.2488 mL 0.6219 mL

    * 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Inquiry Information

    產(chǎn)品名稱：
    Ac-DEVD-CHO
    目錄號：
    HY-P1001
    需求量：