成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

  2. Apoptosis Anti-infection Autophagy
  3. Apoptosis Antibiotic Autophagy Fungal
  4. Valinomycin

Valinomycin  (Synonyms: NSC 122023)

目錄號: HY-N6693 純度: 98.63%
COA 產(chǎn)品使用指南 技術支持

Valinomycin 是一種鉀離子特異性載體，其與鉀離子的復合物通過其疏水表面結合進入生物雙層膜中，破壞膜內(nèi)的正常鉀離子梯度，從而殺傷細胞，結合到脂質(zhì)體中可顯著降低細胞毒性并增強靶向性。Valinomycin 具有抗生素、抗真菌、抗病毒、抗腫瘤、殺蟲等作用，可用于相關研究。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報，我們不為任何個人用途提供產(chǎn)品和服務

Valinomycin Chemical Structure

Valinomycin Chemical Structure

CAS No. : 2001-95-8

1.  客戶無需承擔相應的運輸費用。

2.  同一機構（單位）同一產(chǎn)品試用裝僅限申領一次，同一機構（單位）一年內(nèi)

     可免費申領三個不同產(chǎn)品的試用裝。

3.  試用裝只面向終端客戶。

規(guī)格 價格 是否有貨 數(shù)量
1 mg ￥350
In-stock
2 mg ￥595
In-stock
5 mg ￥1150
In-stock
10 mg ￥1900
In-stock
25 mg ￥3420
In-stock
50 mg   詢價  
100 mg   詢價  

* Please select Quantity before adding items.

Customer Review

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

Valinomycin is a potassium-specific ionophore, the valinomycin-K+ complex can be incorporated into biological bilayer membranes with the hydrophobic surface of valinomycin, destroys the normal K+ gradient across the membrane, and as a result kills the cells, incorporating into liposomes can significantly reduces the cytotoxicity and enhances the targeting effect. Valinomycin exhibits antibiotic, antifungal, antiviral, antitumor and insecticidal efficacy, thus can be used for relevant research[1][2].

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
A2780 IC50
2.18 μM
Compound: 1, VLM
Cytotoxicity against human A2780 cells after 72 hrs by MTT assay
Cytotoxicity against human A2780 cells after 72 hrs by MTT assay
[PMID: 24900628]
BXPC-3 GI50
0.0019 μg/mL
Compound: 3
Cytotoxicity against human BxPC3 cells after 48 hrs by sulforhodamine B assay
Cytotoxicity against human BxPC3 cells after 48 hrs by sulforhodamine B assay
[PMID: 19226154]
C6 IC50
0.0004 μM
Compound: 1, VLM
Cytotoxicity against rat C6 cells after 72 hrs by MTT assay
Cytotoxicity against rat C6 cells after 72 hrs by MTT assay
[PMID: 24900628]
DU-145 GI50
0.0035 μg/mL
Compound: 3
Cytotoxicity against human DU145 cells after 48 hrs by sulforhodamine B assay
Cytotoxicity against human DU145 cells after 48 hrs by sulforhodamine B assay
[PMID: 19226154]
HeLa IC50
0.001 μM
Compound: 17
Antiproliferative activity against HeLa cells after 72 hrs by MTT assay
Antiproliferative activity against HeLa cells after 72 hrs by MTT assay
[PMID: 17300184]
HepG2 IC50
0.0008 μM
Compound: 1, VLM
Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay
[PMID: 24900628]
KM-20L2 GI50
0.0008 μg/mL
Compound: 3
Cytotoxicity against human KM20L2 cells after 48 hrs by sulforhodamine B assay
Cytotoxicity against human KM20L2 cells after 48 hrs by sulforhodamine B assay
[PMID: 19226154]
MCF7 GI50
0.001 μg/mL
Compound: 3
Cytotoxicity against human MCF7 cells after 48 hrs by sulforhodamine B assay
Cytotoxicity against human MCF7 cells after 48 hrs by sulforhodamine B assay
[PMID: 19226154]
MCF7 IC50
0.5 μM
Compound: 17
Antiproliferative activity against MCF7 cells after 72 hrs by MTT assay
Antiproliferative activity against MCF7 cells after 72 hrs by MTT assay
[PMID: 17300184]
MCF7 IC50
1.77 μM
Compound: 1, VLM
Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay
[PMID: 24900628]
MIA PaCa-2 IC50
0.001 μM
Compound: 17
Antiproliferative activity against MiaPaCa2 cells after 72 hrs by MTT assay
Antiproliferative activity against MiaPaCa2 cells after 72 hrs by MTT assay
[PMID: 17300184]
NCI-H460 IC50
0.001 μM
Compound: 17
Antiproliferative activity against H460 cells after 72 hrs by MTT assay
Antiproliferative activity against H460 cells after 72 hrs by MTT assay
[PMID: 17300184]
NCI-H460 GI50
0.0025 μg/mL
Compound: 3
Cytotoxicity against human NCI-H460 cells after 48 hrs by sulforhodamine B assay
Cytotoxicity against human NCI-H460 cells after 48 hrs by sulforhodamine B assay
[PMID: 19226154]
P388 ED50
0.12 μg/mL
Compound: 3
Cytotoxicity against mouse P388 cells after 48 hrs by coulter counter method
Cytotoxicity against mouse P388 cells after 48 hrs by coulter counter method
[PMID: 19226154]
SF-268 GI50
0.0027 μg/mL
Compound: 3
Cytotoxicity against human SF268 cells after 48 hrs by sulforhodamine B assay
Cytotoxicity against human SF268 cells after 48 hrs by sulforhodamine B assay
[PMID: 19226154]
SW-620 IC50
0.0001 μM
Compound: 17
Antiproliferative activity against SW620 cells after 72 hrs by MTT assay
Antiproliferative activity against SW620 cells after 72 hrs by MTT assay
[PMID: 17300184]
Vero EC50
1.63 μM
Compound: 22
Antiviral activity against SARS coronavirus in Vero E6 cells assessed as inhibition of viral replication by ELISA
Antiviral activity against SARS coronavirus in Vero E6 cells assessed as inhibition of viral replication by ELISA
[PMID: 17663539]
Vero CC50
67.5 μM
Compound: 22
Cytotoxicity against Vero E6 cells by MTT assay
Cytotoxicity against Vero E6 cells by MTT assay
[PMID: 17663539]
Vero C1008 CC50
68 μM
Compound: 72
Cytotoxicity against African green monkey Vero E6 cells incubated for 2 days by the MTS assay
Cytotoxicity against African green monkey Vero E6 cells incubated for 2 days by the MTS assay
[PMID: 32845145]
體外研究
(In Vitro)

Lipo-Valinomycin (1-100 μM, 24 h) 劑量依賴性地抑制 HepG2 的細胞活性[2]。
Lipo-Valinomycin (15 μM, 12 h) 破壞線粒體膜電位并誘導 HepG2 的細胞凋亡 (23%)[2]。
1.19
Valinomycin 生物活性[1]

Bioactivity Target Efficacy
Antibacterial Streptococcus pyogenes MIC 0.02 μg/mL
Clostridium sporogenes MIC 8 μg/mL
Enterococcus faecalis MIC 0.39–0.78 μg/disk
Streptococcus pneumoniae MIC 0.39–0.78 μg/disk
Micrococcus luteus MIC 25–50 μg/disk
Antifungal Candida albicans MIC 0.39–0.78 μg/disk
Cryptococcus neoformans MIC 50–100 μg/disk
Phytophthora capsici IC50 15.9 μg/mL
Botrytis cinerea MIC 4 μg/mL
Magnaporthe grisea MIC 4 μg/mL
Candida albicans MIC 32 μg/mL
Colletotrichum gloeosporioides MIC 256 μg/mL
Rhizoctonia solani MIC 256 μg/mL
Penicillium verrucosum IC50 0.005 ng/mL
Antiviral SARS-CoV EC50 0.85 μM
PRRSV IC50 24 nM
RSV IC50 0.0015 μM
MERS-CoV IC50 84 nM
HCoV-OC43 EC50 4.43 μM
HCoV-NL63 EC50 1.89 μM
MHV-A59 EC50 6.78 μM
LACV IC50 588 nM
RVFV MP-12 IC50 41 nM
HRV2 IC50 610 nM
CVB3 IC50 971 nM
ZIKV IC50 78 nM
KEYV IC50 156 nM
HCoV-229E IC50 67 nM
LASV EC50 0.61 μM
LCMV EC50 0.15 μM
Antitumor Human ovarian tumor cells CaOV-3 IC50 0.1 nM
Murine P388 leukemia cancer cells GI50 0.019 μg/mL
Human ovary OVCAR-3 tumor cells GI50 0.19 ng/mL
Brain SF-295 tumor cells GI50 0.35 ng/mL
Renal A-498 carcinoma cells GI50 1.9 ng/mL
Lung NCI-H460 cancer cells GI50 0.21 ng/mL
Colon KM20L2 carcinoma cells GI50 0.27 ng/mL
Melanoma SK-MEL-5 cancer cells GI50 0.26 ng/mL
Rat C6 glioma cells IC50 0.4 μM
Human A2780 ovarian carcinoma cells IC50 2.18 μM
Human MCF-7 breast carcinoma cells IC50 1.77 μM
Human HepG2 hepatocellular carcinoma cells IC50 0.0008 μM
Human U251 glioma cells IC50 7.6 nM

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

Lipo-Valinomycin (1-40 μg/g, 靜脈注射, 每二天, 16 d) 抑制裸鼠內(nèi)的 HepG2 腫瘤生長[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: nude mice with HepG2[2]
Dosage: 1, 2, 5, 10, 20, 40 μg/g
Administration: Intravenous injection (i.v.), every two days for 16 d
Result: Exerted remarkable inhibition effect on tumor growth and no weight loss during the therapeutic period. Caused destructive tumor necrosis as most of the tumor cells were in apoptotic status.
分子量

1111.32

Formula

C54H90N6O18
CAS 號
性狀

固體

顏色

White to off-white

中文名稱

纈氨霉素
結構分類
初始來源

Streptomyces fulvissimus
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性數(shù)據(jù)
細胞實驗： 

Ethanol 中的溶解度 : 50 mg/mL (44.99 mM; 超聲助溶)

DMSO 中的溶解度 : 25 mg/mL (22.50 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 0.8998 mL 4.4992 mL 8.9983 mL
5 mM 0.1800 mL 0.8998 mL 1.7997 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.25 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (2.25 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.05%

參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO / Ethanol 1 mM 0.8998 mL 4.4992 mL 8.9983 mL 22.4958 mL
5 mM 0.1800 mL 0.8998 mL 1.7997 mL 4.4992 mL
10 mM 0.0900 mL 0.4499 mL 0.8998 mL 2.2496 mL
15 mM 0.0600 mL 0.2999 mL 0.5999 mL 1.4997 mL
20 mM 0.0450 mL 0.2250 mL 0.4499 mL 1.1248 mL
Ethanol 25 mM 0.0360 mL 0.1800 mL 0.3599 mL 0.8998 mL
30 mM 0.0300 mL 0.1500 mL 0.2999 mL 0.7499 mL
40 mM 0.0225 mL 0.1125 mL 0.2250 mL 0.5624 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的產(chǎn)品:

Your information is safe with us. * Required Fields.

   產(chǎn)品名稱：

  

* 需求量：

* 客戶姓名：

 

* Email：

* 電話：

 

* 公司或機構名稱：

   留言給我們：

Bulk Inquiry

Inquiry Information

產(chǎn)品名稱：
Valinomycin
目錄號：
HY-N6693
需求量：