Deoxypodophyllotoxin (Synonyms: 脫氧鬼臼毒素)

目錄號: HY-N2500 純度: 99.87%: FAQs
Data Sheet SDS COA 產(chǎn)品使用指南技術(shù)支持

Deoxypodophyllotoxin (DPT)，鬼臼毒素的衍生物，是一種木酚素，從 Anthriscus sylvestris分離，具有強效抗有絲分裂和抗病毒特性。Deoxypodophyllotoxin 作為靶向微管的活性分子，不僅抗有絲分裂劑而且作為有效的血管生成抑制劑，在腫瘤學(xué)中具有重大影響。Deoxypodophyllotoxin 誘導(dǎo)細(xì)胞自噬 (autophagy) 和細(xì)胞凋亡 (apoptosis)。 Deoxypodophyllotoxin 引起 DRG 神經(jīng)元細(xì)胞內(nèi) Ca²⁺ 濃度增加。

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Deoxypodophyllotoxin Chemical Structure

CAS No. : 19186-35-7

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規(guī)格	價格	是否有貨
10 mM * 1 mL in DMSO	￥830	In-stock
1 mg	￥250	In-stock
5 mg	￥750	In-stock
10 mg	￥1200	In-stock
50 mg		詢價
100 mg		詢價

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生物活性
純度 & 產(chǎn)品資料
參考文獻

生物活性

Deoxypodophyllotoxin (DPT), a derivative of podophyllotoxin, is a lignan with potent antimitotic, anti-inflammatory and antiviral properties isolated from Anthriscus sylvestris. Deoxypodophyllotoxin, targets the microtubule, has a major impact in oncology not only as anti-mitotics but also as potent inhibitors of angiogenesis^[1]. Deoxypodophyllotoxin induces cell autophagy and apoptosis^[2]. Deoxypodophyllotoxin evokes increase of intracellular Ca²⁺ concentrations in DRG neurons^[3].

細(xì)胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.004 μg/mL Compound: 10	Antiproliferative activity against human A549 cells assessed as inhibition of cell viability by MTT assay Antiproliferative activity against human A549 cells assessed as inhibition of cell viability by MTT assay	[PMID: 32223924]
A549	IC₅₀	0.47 μM Compound: 2, DPT	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 21733601]
A549	IC₅₀	1.38 μM Compound: 9, DPT	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 22244588]
A549	IC₅₀	1.38 μM Compound: 8, DPT	Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 22041063]
BXPC-3	GI₅₀	0.00044 μg/mL Compound: 1	Growth inhibition of human BxPC3 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human BxPC3 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 26938998]
BXPC-3	ED₅₀	0.0029 μg/mL Compound: 2	Anticancer activity against human BxPC3 cells after 48 hrs by SRB assay Anticancer activity against human BxPC3 cells after 48 hrs by SRB assay	[PMID: 14987061]
DU-145	GI₅₀	0.0017 μg/mL Compound: 1	Growth inhibition of human DU145 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human DU145 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 26938998]
DU-145	ED₅₀	0.0028 μg/mL Compound: 2	Anticancer activity against human DU145 cells after 48 hrs by SRB assay Anticancer activity against human DU145 cells after 48 hrs by SRB assay	[PMID: 14987061]
GLC4 cell line	IC₅₀	0.008 μM Compound: 23	Cytotoxic effect against GLC4 (human small cell lung carcinoma cell line) using the microculture tetrazolium (MTT) assay based on continuous incubation Cytotoxic effect against GLC4 (human small cell lung carcinoma cell line) using the microculture tetrazolium (MTT) assay based on continuous incubation	[PMID: 7783142]
GLC4 cell line	IC₅₀	0.12 μM Compound: 23	Cytotoxic effect against GLC4 (human small cell lung carcinoma cell line) using the microculture tetrazolium (MTT) assay based on 2 hr incubation Cytotoxic effect against GLC4 (human small cell lung carcinoma cell line) using the microculture tetrazolium (MTT) assay based on 2 hr incubation	[PMID: 7783142]
HCT-116	IC₅₀	14.5 nM Compound: DPT	Antiproliferative activity against human HCT-116 cells measured after 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells measured after 72 hrs by MTT assay	[PMID: 36471625]
HeLa	IC₅₀	0.0069 μM Compound: 7	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 21570846]
HeLa	IC₅₀	1.98 μM Compound: 8, DPT	Cytotoxicity against human HeLa cells assessed as growth inhibition after 48 hrs by CCK-8 assay Cytotoxicity against human HeLa cells assessed as growth inhibition after 48 hrs by CCK-8 assay	[PMID: 22041063]
HeLa	IC₅₀	36 μM Compound: 2, DPT	Cytotoxicity against human HeLa cells after 48 hrs by MTT assay Cytotoxicity against human HeLa cells after 48 hrs by MTT assay	[PMID: 21733601]
HeLa	IC₅₀	6.01 μM Compound: 9, DPT	Cytotoxicity against human HeLa cells after 48 hrs by CCK-8 assay Cytotoxicity against human HeLa cells after 48 hrs by CCK-8 assay	[PMID: 22244588]
HepG2	IC₅₀	14.5 μg/mL Compound: 10	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell viability by MTT assay Antiproliferative activity against human HepG2 cells assessed as inhibition of cell viability by MTT assay	[PMID: 32223924]
HL-60	IC₅₀	0.47 μM Compound: 9, DPT	Cytotoxicity against human HL60 cells after 48 hrs by CCK-8 assay Cytotoxicity against human HL60 cells after 48 hrs by CCK-8 assay	[PMID: 22244588]
HL-60	IC₅₀	0.47 μM Compound: 8, DPT	Cytotoxicity against human HL60 cells assessed as growth inhibition after 48 hrs by CCK-8 assay Cytotoxicity against human HL60 cells assessed as growth inhibition after 48 hrs by CCK-8 assay	[PMID: 22041063]
HT-29	IC₅₀	16.6 nM Compound: DPT	Antiproliferative activity against human HT-29 cells measured after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells measured after 72 hrs by MTT assay	[PMID: 36471625]
K562	IC₅₀	16 nM Compound: DPT	Antiproliferative activity against human K562 cells measured after 72 hrs by MTT assay Antiproliferative activity against human K562 cells measured after 72 hrs by MTT assay	[PMID: 36471625]
KB	IC₅₀	0.003 μM Compound: Dppt	Cytotoxicity against human KB cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay Cytotoxicity against human KB cells assessed as inhibition of cell proliferation after 72 hrs by MTS assay	[PMID: 22429510]
KB	IC₅₀	0.0089 μM Compound: 7	Cytotoxicity against human KB cells after 72 hrs by MTT assay Cytotoxicity against human KB cells after 72 hrs by MTT assay	[PMID: 21570846]
KM-20L2	GI₅₀	0.0014 μg/mL Compound: 1	Growth inhibition of human KM20L2 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human KM20L2 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 26938998]
KM-20L2	ED₅₀	0.0028 μg/mL Compound: 2	Anticancer activity against human KM20L2 cells after 48 hrs by SRB assay Anticancer activity against human KM20L2 cells after 48 hrs by SRB assay	[PMID: 14987061]
MCF7	ED₅₀	0.0021 μg/mL Compound: 2	Anticancer activity against human MCF7 cells after 48 hrs by SRB assay Anticancer activity against human MCF7 cells after 48 hrs by SRB assay	[PMID: 14987061]
MCF7	GI₅₀	0.0027 μg/mL Compound: 1	Growth inhibition of human MCF7 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human MCF7 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 26938998]
MCF7	IC₅₀	6 nM Compound: DPT	Antiproliferative activity against human MCF7 cells measured after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells measured after 72 hrs by MTT assay	[PMID: 36471625]
MDA-MB-231	IC₅₀	0.07 μg/mL Compound: 10	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability by MTT assay	[PMID: 32223924]
NCI-H460	GI₅₀	0.0018 μg/mL Compound: 1	Growth inhibition of human NCI-H460 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human NCI-H460 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 26938998]
NCI-H460	ED₅₀	0.0027 μg/mL Compound: 2	Anticancer activity against human NCI-H460 cells after 48 hrs by SRB assay Anticancer activity against human NCI-H460 cells after 48 hrs by SRB assay	[PMID: 14987061]
P388	ED₅₀	0.0023 μg/mL Compound: 2	Anticancer activity against mouse P388 cells after 48 hrs Anticancer activity against mouse P388 cells after 48 hrs	[PMID: 14987061]
P388	ED₅₀	0.029 μg/mL Compound: 1	Growth inhibition of mouse P388 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of mouse P388 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 26938998]
PC-3	IC₅₀	0.018 μM Compound: 8	Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells after 72 hrs by MTT assay	[PMID: 27214307]
SF-268	GI₅₀	0.00056 μg/mL Compound: 1	Growth inhibition of human SF268 cells measured after 48 hrs by sulforhodamine B assay Growth inhibition of human SF268 cells measured after 48 hrs by sulforhodamine B assay	[PMID: 26938998]
SF-268	ED₅₀	0.0033 μg/mL Compound: 2	Anticancer activity against human SF268 cells after 48 hrs by SRB assay Anticancer activity against human SF268 cells after 48 hrs by SRB assay	[PMID: 14987061]
SiHa	IC₅₀	1.98 μM Compound: 9, DPT	Cytotoxicity against human SiHa cells after 48 hrs by MTT assay Cytotoxicity against human SiHa cells after 48 hrs by MTT assay	[PMID: 22244588]
SiHa	IC₅₀	10.2 μM Compound: 2, DPT	Cytotoxicity against human SiHa cells after 48 hrs by MTT assay Cytotoxicity against human SiHa cells after 48 hrs by MTT assay	[PMID: 21733601]
SiHa	IC₅₀	6.01 μM Compound: 8, DPT	Cytotoxicity against human SiHa cells assessed as growth inhibition after 48 hrs by MTT assay Cytotoxicity against human SiHa cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 22041063]

體外研究
(In Vitro)

Deoxypodophyllotoxin (25-75 nM; 6-48 hours) increases the percentage of early apoptotic cell population from 2.05 to 5.62 and 18.49% for 24 h and 48 h, respectively^[1].
Deoxypodophyllotoxin (25-75 nM; 6-48 hours) treats SGC-7901 cells arrested in G2/M phase in time- and dose- dependent manners^[1].
Deoxypodophyllotoxin (25-75 nM; 6-48 hours) results in a remarkably time- and dose-dependent decrease in Cdc2 and Cdc25C expression levels and increases cyclin B1 within 6h, decreases PARP, Bcl-2 and caspase-3 activity^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	SGC-7901 cells
Concentration:	25, 50, 75 nM
Incubation Time:	6, 12, 24, 48 hours
Result:	Induced apoptosis in SGC-7901 Cells.

Cell Cycle Analysis^[1]

Cell Line:	SGC-7901 cells
Concentration:	25, 50, 75 nM
Incubation Time:	6, 12, 24, 48 hours
Result:	Induced G2/M cell cycle arrest in SGC-7901 Cells

Western Blot Analysis^[1]

Cell Line:	SGC-7901 cells
Concentration:	25, 50, 75 nM
Incubation Time:	6, 12, 24, 48 hours
Result:	Altered the expression of cyclin B1, Cdc2,Cdc25C, p-PARP, Bcl-2 and p-caspase-3 proteins.

體內(nèi)研究
(In Vivo)

Deoxypodophyllotoxin (intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days) suppresses the tumors in a dose-dependent manner, the growth of tumors is inhibited by 22.19%, 47.91% and 50.93% with DPT at 5, 10 and 20 mg/kg, respectively^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xenograft model of gastric cancer in nude mice with SGC-7901 cells^[1]
Dosage:	5, 10, and 20 mg/kg
Administration:	Intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days
Result:	Inhibited the growth of gastric cancer tumors.

分子量

398.41

Formula

C₂₂H₂₂O₇

CAS 號

19186-35-7

性狀

固體

顏色

White to off-white

中文名稱

脫氧鬼臼毒素

結(jié)構(gòu)分類

初始來源

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性數(shù)據(jù)

細(xì)胞實驗：

DMSO 中的溶解度 : 100 mg/mL (251.00 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	2.5100 mL	12.5499 mL	25.0998 mL
5 mM	0.5020 mL	2.5100 mL	5.0200 mL
10 mM	0.2510 mL	1.2550 mL	2.5100 mL

查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

摩爾計算器
稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動物實驗：

請根據(jù)您的實驗動物和給藥方式選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

方案一

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.27 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請依序添加每種溶劑： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.27 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液中，混合均勻。
2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
方案三

請依序添加每種溶劑： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.27 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

動物溶解方案計算器

請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

每只動物的給藥體積

μL

動物數(shù)量

只

由于實驗過程有損耗，建議您多配一只動物的量

請輸入您的動物體內(nèi)配方組成：

DMSO +

Tween-80 +

Saline

如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。

方案所需 助溶劑 包括：DMSO，，均可在 MCE 網(wǎng)站選購。，Tween 80，均可在 MCE 網(wǎng)站選購。

計算結(jié)果

工作液所需濃度 : mg/mL

儲備液配制方法 : mg 藥物溶于 μL DMSO（母液濃度為 mg/mL）。

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。

動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。

連續(xù)給藥周期超過半月以上，請謹(jǐn)慎選擇該方案。

請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱（超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。

純度 & 產(chǎn)品資料

純度: 99.87%

選擇批次：

Data Sheet (638 KB) SDS (392 KB)

COA (202 KB) HNMR (263 KB) LCMS (94 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻

[1]. Wang YR, et al. Deoxypodophyllotoxin induces G2/M cell cycle arrest and apoptosis in SGC-7901 cells and inhibits tumor growth in vivo. Molecules. 2015 Jan 20;20(1):1661-75. [Content Brief]

[2]. Kim SH, et al. Deoxypodophyllotoxin induces cytoprotective autophagy against apoptosis via inhibition of PI3K/AKT/mTOR pathway in osteosarcoma U2OS cells. Pharmacol Rep. 2017 Oct;69(5):878-884. [Content Brief]

[3]. Xu P, et al. Pharmacological effect of deoxypodophyllotoxin: a medicinal agent of plant origin, on mammalian neurons. Neurotoxicology. 2010 Dec;31(6):680-6. [Content Brief]

Deoxypodophyllotoxin 相關(guān)分類

Infection Cardiovascular Disease

完整儲備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5100 mL	12.5499 mL	25.0998 mL	62.7494 mL
	5 mM	0.5020 mL	2.5100 mL	5.0200 mL	12.5499 mL
	10 mM	0.2510 mL	1.2550 mL	2.5100 mL	6.2749 mL
	15 mM	0.1673 mL	0.8367 mL	1.6733 mL	4.1833 mL
	20 mM	0.1255 mL	0.6275 mL	1.2550 mL	3.1375 mL
	25 mM	0.1004 mL	0.5020 mL	1.0040 mL	2.5100 mL
	30 mM	0.0837 mL	0.4183 mL	0.8367 mL	2.0916 mL
	40 mM	0.0627 mL	0.3137 mL	0.6275 mL	1.5687 mL
	50 mM	0.0502 mL	0.2510 mL	0.5020 mL	1.2550 mL
	60 mM	0.0418 mL	0.2092 mL	0.4183 mL	1.0458 mL
	80 mM	0.0314 mL	0.1569 mL	0.3137 mL	0.7844 mL
	100 mM	0.0251 mL	0.1255 mL	0.2510 mL	0.6275 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Deoxypodophyllotoxin19186-35-7脫氧鬼臼毒素Microtubule/TubulinApoptosisAutophagyInhibitorinhibitorinhibit

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Deoxypodophyllotoxin (Synonyms: 脫氧鬼臼毒素)

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Deoxypodophyllotoxin 相關(guān)產(chǎn)品

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Deoxypodophyllotoxin (Synonyms: 脫氧鬼臼毒素)

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Deoxypodophyllotoxin 相關(guān)抗體

Deoxypodophyllotoxin 相關(guān)產(chǎn)品

•相關(guān)化合物庫:

•同靶點產(chǎn)品:

•同靶點蛋白產(chǎn)品:

生物活性

純度 & 產(chǎn)品資料

參考文獻

Deoxypodophyllotoxin 相關(guān)抗體:

摩爾計算器

稀釋計算器

Deoxypodophyllotoxin 相關(guān)分類

完整儲備液配制表

Keywords:

您最近查看的產(chǎn)品:

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