Imperatorin (Synonyms: 歐前胡素; Ammidin)

目錄號: HY-N0285 純度: 98.72%: FAQs
Data Sheet SDS COA 產(chǎn)品使用指南技術(shù)支持

Imperatorin 是一種有效的 NO synthesis 抑制劑，IC₅₀ 為 9.2 μmol，也是 BChE 抑制劑，IC₅₀ 為 31.4 μmol。Imperatorin 是 TRPV1 的微弱激動劑，EC₅₀ 為 12.6±3.2 μM。

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Imperatorin Chemical Structure

CAS No. : 482-44-0

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規(guī)格	價格	是否有貨
10 mM * 1 mL in DMSO	￥550	In-stock
25 mg	￥500	In-stock
50 mg	￥800	In-stock
100 mg	￥1300	In-stock
200 mg		詢價
500 mg		詢價

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Customer Review

Other Forms of Imperatorin:

Imperatorin-d₆ 詢價
Imperatorin (Standard) 詢價

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻

•Proc Natl Acad Sci U S A. 2023 Sep 12;120(37):e2308685120. [Abstract]
•bioRxiv. 2023 Jun 15.

Imperatorin 相關(guān)產(chǎn)品

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AChE BChE

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TRPC TRPM TRPV TRPA TRPML

生物活性
實驗參考方法
純度 & 產(chǎn)品資料
參考文獻

生物活性

Imperatorin is an effective of NO synthesis inhibitor (IC₅₀=9.2 μmol), which also is a BChE inhibitor (IC₅₀=31.4 μmol). Imperatorin is a weak agonist of TRPV1 with EC₅₀ of 12.6±3.2 μM.

IC₅₀ & Target

AChE

BChE

細胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 20 μg/mL Compound: 13	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 29975532]
HepG2	IC₅₀	> 20 μg/mL Compound: 13	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 29975532]
MDA-MB-231	IC₅₀	> 20 μg/mL Compound: 13	Cytotoxicity against human MDA-MB-231 cells by MTT assay Cytotoxicity against human MDA-MB-231 cells by MTT assay	[PMID: 29975532]
Neutrophil	IC₅₀	> 10 μM Compound: 15	Antiinflammatory activity in human neutrophil assessed as inhibition of FMLP/CB-induced elastase release for 5 mins Antiinflammatory activity in human neutrophil assessed as inhibition of FMLP/CB-induced elastase release for 5 mins	[PMID: 24798144]
Neutrophil	IC₅₀	> 10 μM Compound: 13	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release preincubated for 5 mins followed by fMLP/CB stimulation and measured for 10 mins using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release preincubated for 5 mins followed by fMLP/CB stimulation and measured for 10 mins using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as substrate	[PMID: 29975532]
Neutrophil	IC₅₀	0.2 μM Compound: 13	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation preincubated for 5 mins followed by cytochalasin B/fMLP addition for 3 mins by superoxide dismutase inhibitable reduction of ferricytochro Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation preincubated for 5 mins followed by cytochalasin B/fMLP addition for 3 mins by superoxide dismutase inhibitable reduction of ferricytochro	[PMID: 29975532]
Neutrophil	IC₅₀	3.5 μM Compound: 15	Antiinflammatory activity in human neutrophil assessed as inhibition of FMLP/CB-induced superoxide anion generation pretreated for 5 mins by spectrophotometry Antiinflammatory activity in human neutrophil assessed as inhibition of FMLP/CB-induced superoxide anion generation pretreated for 5 mins by spectrophotometry	[PMID: 24798144]
SH-SY5Y	EC₅₀	> 50 μM Compound: 33	Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 1-methyl-4-phenylpyridinium ion-induced cell death after 48 hrs by MTT assay Neuroprotective activity in human SH-SY5Y cells assessed as reduction in 1-methyl-4-phenylpyridinium ion-induced cell death after 48 hrs by MTT assay	[PMID: 27420919]
SH-SY5Y	EC₅₀	8.4 μM Compound: 33	Neuroprotective activity in human SH-SY5Y cells assessed as reduction in oxygen glucose deprivation-induced cell death incubated for 16 hrs under hypoxic condition followed by incubation for 24 hrs under normoxic condition by MTT assay Neuroprotective activity in human SH-SY5Y cells assessed as reduction in oxygen glucose deprivation-induced cell death incubated for 16 hrs under hypoxic condition followed by incubation for 24 hrs under normoxic condition by MTT assay	[PMID: 27420919]

體外研究
(In Vitro)

Imperatorin 是一種植物次生代謝產(chǎn)物，屬于香豆素類，特別是呋喃香豆素類。Imperatorin 通過 α₁β₂γ_2S 受體增強 GABA 誘導(dǎo)的氯離子電流（I_GABA）。Imperatorin 對 I_GABA 的增強作用在 100 μmol 時分別為 50.5 %和 300 μmol 時為 109.8 %。Imperatorin 和 Phellopterin，發(fā)現(xiàn)于白芷的根部，在體外抑制 [³H] 地西泮與大鼠腦 GABA_A 受體的苯二氮?類位點結(jié)合，Imperatorin 的 IC₅₀ 為 12.3 μmol，而 Phellopterin 為 400 nmol。Imperatorin 在 3.5 ~ 14 mmol 濃度范圍內(nèi)，通過不可逆地與 GABA-T 活性位點結(jié)合，以時間和濃度依賴性方式顯著地不可逆地抑制 GABA-T。Imperatorin 是一種可逆性乙酰膽堿酯酶（AChE）抑制劑，其作用呈劑量依賴性。采用分光光度法檢測 Imperatorin 和當歸果實粗提物在12.5、25、50、100 μg/mL 濃度下的 AChE 和 BChE 抑制活性。Imperatorin 對 AChE 的抑制率為 13.75 ~ 46.11%，而對BChE的抑制率為37.46 ~ 83.98%。Imperatorin 對 BChE 而非 AChE 具有選擇性，其對 BChE 的 IC₅₀為 31.4 μmol。Imperatorin 和 (+)-Byakangelicol 被發(fā)現(xiàn)是最有效的 BACE-1 抑制劑，其 IC₅₀s 分別為 91.8 和104.9 μmol。Imperatorin（IC₅₀=9.2 μmol）也能有效抑制 NO 合成^[1]。
Imperatorin 是 TRPV1 的弱激動劑， TRPV1 參與檢測多種傷害性刺激，表現(xiàn)為EC₅₀為12.6±3.2 μM^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

Imperatorin （10, 20 mg/kg）注射后30分鐘表現(xiàn)出抗焦慮作用，并改善了記憶和學習過程的不同階段，包括獲得和鞏固^[1]。
Imperatorin（10, 20 mg/kg) 可以降低尼古丁（0.1 mg/kg，s.c）的焦慮效應(yīng)^[1]。
Imperatorin（30, 40 mg/kg, i.p. )著增強卡馬西平對電休克誘導(dǎo)的最大驚厥的抗驚厥活性，分別將ED₅₀從10.8 mg/kg降至6.8 mg/kg（34%）和6 mg/kg（42%）^[1]。
Imperatorin（10, 20 mg/kg) 和卡馬西平6.8 mg/kg 使卡馬西平的腦總濃度從1.260 μg/mL增加到2.328 μg/mL（增加了85%），這可能是通過改變血屏障通透性或像多藥耐藥蛋白的抑制劑引起的^[1]。
Imperatorin 可使γ -氨基丁酸轉(zhuǎn)氨酶失活并抑制乙酰膽堿酯酶活性^[1]。
Imperatorin（5和10 mg/kg）急性給藥可改善東莨菪堿損害的記憶獲取和鞏固, 在注射東莨菪堿（1 mg/kg）之前^[1]。
Imperatorin （10 mg/kg, 7天，每天2次）顯著減弱東莨菪堿對記憶獲取的影響，而當測定記憶鞏固時，5和10 mg/kg的 Imperatorin 有效^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

270.28

Formula

C₁₆H₁₄O₄

CAS 號

482-44-0

性狀

固體

顏色

White to off-white

中文名稱

歐前胡素；白茅苷；前胡素；主人草素；白芷乙素；歐芹屬素乙；前胡內(nèi)酯

結(jié)構(gòu)分類

初始來源

運輸條件

Room temperature in continental US; may vary elsewhere.

儲存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性數(shù)據(jù)

細胞實驗：

DMSO 中的溶解度 : 50 mg/mL (184.99 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

配制儲備液

濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg

1 mM	3.6999 mL	18.4993 mL	36.9987 mL
5 mM	0.7400 mL	3.6999 mL	7.3997 mL
10 mM	0.3700 mL	1.8499 mL	3.6999 mL

查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲存時，請在2年內(nèi)使用， -20°C儲存時，請在1年內(nèi)使用。

摩爾計算器
稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

濃度 _(start)

體積 _(start)

濃度 _(final)

體積 _(final)

動物實驗：

請根據(jù)您的實驗動物和給藥方式選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

方案一

請依序添加每種溶劑： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.25 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水定容至 1 mL。
生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
方案二

請依序添加每種溶劑： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.25 mM); 澄清溶液

此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液，此方案實驗周期在半個月以上的動物實驗酌情使用。
以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL玉米油中，混合均勻。

動物溶解方案計算器

請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

每只動物的給藥體積

μL

動物數(shù)量

只

由于實驗過程有損耗，建議您多配一只動物的量

請輸入您的動物體內(nèi)配方組成：

DMSO +

Tween-80 +

Saline

如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。

方案所需 助溶劑 包括：DMSO，，均可在 MCE 網(wǎng)站選購。，Tween 80，均可在 MCE 網(wǎng)站選購。

計算結(jié)果

工作液所需濃度 : mg/mL

儲備液配制方法 : mg 藥物溶于 μL DMSO（母液濃度為 mg/mL）。

您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。

動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。

連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。

請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱（超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。

純度 & 產(chǎn)品資料

純度: 98.72%

選擇批次：

Data Sheet (668 KB) SDS (393 KB)

COA (293 KB) HNMR (251 KB) LCMS (94 KB)

產(chǎn)品使用指南 (1538 KB)

參考文獻

[1]. Kozio? E, et al. Imperatorin-pharmacological meaning and analytical clues: profound investigation. Phytochem Rev. 2016;15:627-649. [Content Brief]

[2]. Chen X, et al. Furanocoumarins are a novel class of modulators for the transient receptor potential vanilloid type 1 (TRPV1) channel. J Biol Chem. 2014 Apr 4;289(14):9600-10. [Content Brief]

[3]. Budzynska B, et al. Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice. Psychopharmacology (Berl). 2015 Mar;232(5):931-42. [Content Brief]

Animal Administration ^[3]	Mice^[3] The experiments are carried out on naive male Swiss mice weighing 20-25 g. Drugs are administered intraperitoneally (i.p.) at the volume of 10 mL/kg. Fresh drug solutions are prepared on each day of experimentation. Control groups receive saline injections of the same volume and via the same route of administration. During the acute treatment, the animals are allocated to the following drug groups: saline, rivastigmine (0.5 mg/kg, i.p.), scopolamine (1 mg/kg, i.p.), Imperatorin (1, 5, 10 mg/kg, i.p.), or Imperatorin coadministered with scopolamine. To measure the memory acquisition processes, scopolamine is administered 20 min before the pretest, whereas Imperatorin and rivastigmine are administered 30 min before the pretest. To measure the memory consolidation processes, rivastigmine or scopolamine (1 mg/kg) is administered immediately after the pretest, whereas Imperatorin is administered 15 min after pretest or after scopolamine injection. On the second day, the mice are retested. In the second set of the experiments, animals are randomly allocated to receive 6 days of i.p. injections of Imperatorin (1, 5, and 10 mg/kg, i.p.) or saline, twice daily (8:00 a.m. and 8:00 p.m.). On the seventh day, these animals are subjected to saline, scopolamine (1 mg/kg, i.p.), Imperatorin (1, 5, and 10 mg/kg, i.p.), or Imperatorin coadministered with scopolamine. To measure the memory acquisition processes, scopolamine is administered 20 min before the pretest and Imperatorin 30 min before the pretest. To measure the memory consolidation processes, scopolamine (1 mg/kg) is administered immediately after the pretest, whereas Imperatorin is administered 15 min after the pretest or after scopolamine injection. On the eighth day, the mice are retested. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻	[1]. Kozio? E, et al. Imperatorin-pharmacological meaning and analytical clues: profound investigation. Phytochem Rev. 2016;15:627-649. [Content Brief] [2]. Chen X, et al. Furanocoumarins are a novel class of modulators for the transient receptor potential vanilloid type 1 (TRPV1) channel. J Biol Chem. 2014 Apr 4;289(14):9600-10. [Content Brief] [3]. Budzynska B, et al. Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice. Psychopharmacology (Berl). 2015 Mar;232(5):931-42. [Content Brief]

Animal Administration
^[3]

Mice^[3]
The experiments are carried out on naive male Swiss mice weighing 20-25 g. Drugs are administered intraperitoneally (i.p.) at the volume of 10 mL/kg. Fresh drug solutions are prepared on each day of experimentation. Control groups receive saline injections of the same volume and via the same route of administration. During the acute treatment, the animals are allocated to the following drug groups: saline, rivastigmine (0.5 mg/kg, i.p.), scopolamine (1 mg/kg, i.p.), Imperatorin (1, 5, 10 mg/kg, i.p.), or Imperatorin coadministered with scopolamine. To measure the memory acquisition processes, scopolamine is administered 20 min before the pretest, whereas Imperatorin and rivastigmine are administered 30 min before the pretest. To measure the memory consolidation processes, rivastigmine or scopolamine (1 mg/kg) is administered immediately after the pretest, whereas Imperatorin is administered 15 min after pretest or after scopolamine injection. On the second day, the mice are retested. In the second set of the experiments, animals are randomly allocated to receive 6 days of i.p. injections of Imperatorin (1, 5, and 10 mg/kg, i.p.) or saline, twice daily (8:00 a.m. and 8:00 p.m.). On the seventh day, these animals are subjected to saline, scopolamine (1 mg/kg, i.p.), Imperatorin (1, 5, and 10 mg/kg, i.p.), or Imperatorin coadministered with scopolamine. To measure the memory acquisition processes, scopolamine is administered 20 min before the pretest and Imperatorin 30 min before the pretest. To measure the memory consolidation processes, scopolamine (1 mg/kg) is administered immediately after the pretest, whereas Imperatorin is administered 15 min after the pretest or after scopolamine injection. On the eighth day, the mice are retested.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

參考文獻

[1]. Kozio? E, et al. Imperatorin-pharmacological meaning and analytical clues: profound investigation. Phytochem Rev. 2016;15:627-649. [Content Brief]

[2]. Chen X, et al. Furanocoumarins are a novel class of modulators for the transient receptor potential vanilloid type 1 (TRPV1) channel. J Biol Chem. 2014 Apr 4;289(14):9600-10. [Content Brief]

[3]. Budzynska B, et al. Effects of imperatorin on scopolamine-induced cognitive impairment and oxidative stress in mice. Psychopharmacology (Berl). 2015 Mar;232(5):931-42. [Content Brief]

Imperatorin 相關(guān)分類

Neurological Disease

完整儲備液配制表

可選溶劑	濃度溶劑體積質(zhì)量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.6999 mL	18.4993 mL	36.9987 mL	92.4967 mL
	5 mM	0.7400 mL	3.6999 mL	7.3997 mL	18.4993 mL
	10 mM	0.3700 mL	1.8499 mL	3.6999 mL	9.2497 mL
	15 mM	0.2467 mL	1.2333 mL	2.4666 mL	6.1664 mL
	20 mM	0.1850 mL	0.9250 mL	1.8499 mL	4.6248 mL
	25 mM	0.1480 mL	0.7400 mL	1.4799 mL	3.6999 mL
	30 mM	0.1233 mL	0.6166 mL	1.2333 mL	3.0832 mL
	40 mM	0.0925 mL	0.4625 mL	0.9250 mL	2.3124 mL
	50 mM	0.0740 mL	0.3700 mL	0.7400 mL	1.8499 mL
	60 mM	0.0617 mL	0.3083 mL	0.6166 mL	1.5416 mL
	80 mM	0.0462 mL	0.2312 mL	0.4625 mL	1.1562 mL
	100 mM	0.0370 mL	0.1850 mL	0.3700 mL	0.9250 mL

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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Imperatorin482-44-0Ammidin歐前胡素白茅苷前胡素主人草素白芷乙素歐芹屬素乙前胡內(nèi)酯Cholinesterase (ChE)TRP ChannelTransient receptor potential channelsInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只為有資質(zhì)的科研機構(gòu)、醫(yī)藥企業(yè)基于科學研究或藥證申報的用途提供醫(yī)藥研發(fā)服務(wù)，不為任何個人或者非科研性質(zhì)的、非用于藥證申報使用等其他用途提供服務(wù)。

站點地圖隱私聲明

滬ICP備15051369號-4

上海工商

滬公網(wǎng)安備31011502019417

滬(浦)應(yīng)急管危經(jīng)許[2021]201709(QFYS)

營業(yè)執(zhí)照（三證合一）

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Imperatorin (Synonyms: 歐前胡素; Ammidin)

Customer Review

Other Forms of Imperatorin:

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻