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  2. PI3K/Akt/mTOR Anti-infection Apoptosis Autophagy
  3. mTOR Influenza Virus Apoptosis Autophagy
  4. Aloe emodin

Aloe emodin  (Synonyms: 蘆薈大黃素; Rhabarberone; 3-Hydroxymethylchrysazine)

目錄號(hào): HY-N0189 純度: 98.32%
COA 產(chǎn)品使用指南

Aloe emodin (Rhabarberone) 是一種天然羥基蒽醌，具有抗腫瘤活性。 Aloe emodin (Rhabarberone) 可以與 mTORC2 結(jié)合并抑制其激酶活性。 Aloe emodin (Rhabarberone) 發(fā)揮抗增殖作用并誘導(dǎo)細(xì)胞凋亡 (apoptosis)。Aloe emodin (Rhabarberone) 還表現(xiàn)出針對(duì)甲型流感病毒 (influenza A virus) 的抗病毒活性。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Aloe emodin Chemical Structure

Aloe emodin Chemical Structure

CAS No. : 481-72-1

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥902
In-stock
100 mg ￥820
In-stock
500 mg ￥2050
In-stock
1 g   詢價(jià)  
5 g   詢價(jià)  

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Customer Review

Other Forms of Aloe emodin:

MCE 顧客使用本產(chǎn)品發(fā)表的 2 篇科研文獻(xiàn)

查看 mTOR 亞型特異性產(chǎn)品:

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Aloe emodin (Rhabarberone) is a natural hydroxyanthraquinone with antitumor activities. aloe-emodin can bind with mTORC2 and inhibit its kinase activity. Aloe emodin exerts antiproliferation effects and induces cellular apoptosis[1]. Aloe emodin also exhibits antiviral activity that against influenza A virus[2].

IC50 & Target[1]

mTORC2

 

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
B16-F10 IC50
3.12 μM
Compound: 1
Anticancer activity against mouse B16-F10 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Anticancer activity against mouse B16-F10 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34124677]
HCT-116 IC50
8.7 μM
Compound: 4
Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
[PMID: 17949858]
HEK293 IC50
1 μM
Compound: 1
Cytotoxicity against human HEK293 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Cytotoxicity against human HEK293 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34124677]
HepG2 IC50
10 μM
Compound: 4
Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
[PMID: 17949858]
HepG2 IC50
6.13 μM
Compound: 1
Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Anticancer activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34124677]
Huh-7 CC50
> 50 μM
Compound: 25
Cytotoxicity against human Huh7.5.1 cells by MTT assay
Cytotoxicity against human Huh7.5.1 cells by MTT assay
[PMID: 22445328]
Huh-7 EC50
20.8 μM
Compound: 25
Antiviral activity against HCV JFH-1 J399EM infected in Human Huh7.5.1 cells assessed as suppression of viral replication after 72 hrs by EGFP assay
Antiviral activity against HCV JFH-1 J399EM infected in Human Huh7.5.1 cells assessed as suppression of viral replication after 72 hrs by EGFP assay
[PMID: 22445328]
MCF7 IC50
> 20 μM
Compound: AE
Cytotoxicity against human MCF7 cells after 24 hrs by XTT assay
Cytotoxicity against human MCF7 cells after 24 hrs by XTT assay
[PMID: 24900344]
MCF7 IC50
4.14 μM
Compound: 1
Anticancer activity against human MCF-7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Anticancer activity against human MCF-7 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34124677]
MDA-MB-231 IC50
12.24 μM
Compound: 1
Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
Anticancer activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay
[PMID: 34124677]
MOLT-4 IC50
> 20 μM
Compound: AE
Cytotoxicity against human MOLT4 cells after 24 hrs by XTT assay
Cytotoxicity against human MOLT4 cells after 24 hrs by XTT assay
[PMID: 24900344]
OVCAR-3 IC50
> 20 μM
Compound: AE
Cytotoxicity against human OVCAR3 cells after 24 hrs by XTT assay
Cytotoxicity against human OVCAR3 cells after 24 hrs by XTT assay
[PMID: 24900344]
SK-OV-3 IC50
> 20 μM
Compound: AE
Cytotoxicity against human SKOV3 cells after 24 hrs by XTT assay
Cytotoxicity against human SKOV3 cells after 24 hrs by XTT assay
[PMID: 24900344]
體外研究
(In Vitro)

Aloe emodin （0-15 μM；24-96 小時(shí)）以劑量依賴性方式抑制 PC3 細(xì)胞增殖[1]。
Aloe emodin （0-15 μM；24 小時(shí)）抑制 mTORC2 下游底物 Akt 和 PKCα 的激活。Aloe emodin 可以與細(xì)胞內(nèi)的 mTORC2 結(jié)合并抑制其激酶活性[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: PC3 cells
Concentration: 0 μM, 2.5 μM, 5 μM, 10 μM or 15 μM
Incubation Time: 24 h, 48 h, 72 h or 96 h
Result: Suppressed proliferation and anchorage-independent growth of PC3 cells.

Western Blot Analysis[1]

Cell Line: PC3 cells
Concentration: 0 μM, 2.5 μM, 5 μM, 10 μM or 15 μM
Incubation Time: 24 h
Result: Inhibited the activation of the downstream substrates of mTORC2, Akt and PKCα.
體內(nèi)研究
(In Vivo)

Aloe emodin (10-50 mg/kg) 通過(guò)抑制 mTOR 復(fù)合物 2 活性來(lái)抑制腫瘤生長(zhǎng)[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic nude mice (BALB/c nude mouse, 6 weeks old)[1]
Dosage: 10 mg/kg, 50 mg/kg (20% PEG400 in autoclaved PBS)
Administration: Intraperitoneal injection, five times per week; for 28 days
Result: Exhibited tumor suppression effects in vivo in an athymic nude mouse model.
分子量

270.24

Formula

C15H10O5
CAS 號(hào)
性狀

固體

中文名稱

蘆薈大黃素；蘆薈瀉素
結(jié)構(gòu)分類
初始來(lái)源
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : ≥ 20.83 mg/mL (77.08 mM; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開(kāi)封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.7004 mL 18.5021 mL 37.0041 mL
5 mM 0.7401 mL 3.7004 mL 7.4008 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn)：

請(qǐng)根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請(qǐng)先按照 In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過(guò)程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過(guò)加熱和/或超聲的方式助溶

  • 方案 一

    請(qǐng)依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.08 mg/mL (7.70 mM); 懸濁液; 超聲助溶

    此方案可獲得 2.08 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請(qǐng)依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.08 mg/mL (7.70 mM); 懸濁液; 超聲助溶

    此方案可獲得 2.08 mg/mL的均勻懸濁液，懸濁液可用于口服和腹腔注射。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲(chǔ)備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過(guò)程有損耗，建議您多配一只動(dòng)物的量
請(qǐng)輸入您的動(dòng)物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動(dòng)物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過(guò) 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購(gòu)。 ，Tween 80，均可在 MCE 網(wǎng)站選購(gòu)。
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
儲(chǔ)備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲(chǔ)備液濃度超過(guò)該產(chǎn)品的實(shí)測(cè)溶解度，以下方案僅供參考，如有需要，請(qǐng)與 MCE 中國(guó)技術(shù)支持聯(lián)系。
動(dòng)物實(shí)驗(yàn)體內(nèi)工作液的配制方法 : 取 μL DMSO 儲(chǔ)備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過(guò)半月以上，請(qǐng)謹(jǐn)慎選擇該方案。
請(qǐng)確保第一步儲(chǔ)備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 98.32%

參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.7004 mL 18.5021 mL 37.0041 mL 92.5104 mL
5 mM 0.7401 mL 3.7004 mL 7.4008 mL 18.5021 mL
10 mM 0.3700 mL 1.8502 mL 3.7004 mL 9.2510 mL
15 mM 0.2467 mL 1.2335 mL 2.4669 mL 6.1674 mL
20 mM 0.1850 mL 0.9251 mL 1.8502 mL 4.6255 mL
25 mM 0.1480 mL 0.7401 mL 1.4802 mL 3.7004 mL
30 mM 0.1233 mL 0.6167 mL 1.2335 mL 3.0837 mL
40 mM 0.0925 mL 0.4626 mL 0.9251 mL 2.3128 mL
50 mM 0.0740 mL 0.3700 mL 0.7401 mL 1.8502 mL
60 mM 0.0617 mL 0.3084 mL 0.6167 mL 1.5418 mL
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產(chǎn)品名稱：
Aloe emodin
目錄號(hào)：
HY-N0189
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