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  1. JAK/STAT Signaling Stem Cell/Wnt Epigenetics
  2. STAT MicroRNA
  3. Diosgenin

Diosgenin  (Synonyms: 薯蕷皂素)

目錄號: HY-N0177 純度: 99.35%
COA 產(chǎn)品使用指南 技術支持

Diosgenin,一種甾體皂苷,能夠抑制 STAT3 信號通路。Diosgenin 是 Pdia3/ERp57 的激活劑。Diosgenin 通過抑制巨噬細胞 miR-19b 表達抑制主動脈粥樣硬化進展。

MCE 的所有產(chǎn)品僅用作科學研究或藥證申報,我們不為任何個人用途提供產(chǎn)品和服務

Diosgenin Chemical Structure

Diosgenin Chemical Structure

CAS No. : 512-04-9

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100 mg ¥350
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500 mg ¥560
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Customer Review

Other Forms of Diosgenin:

    Diosgenin purchased from MCE. Usage Cited in: Cell Physiol Biochem. 2016;39(4):1626-37.  [Abstract]

    Arithmetic means ± SEM (n = 10) of erythrocyte annexin-V-binding following incubation for 48 hours to Ringer solution without (white bar) or with (black bars) Diosgenin (5-15 μM).

    查看 STAT 亞型特異性產(chǎn)品:

    • 生物活性

    • 純度 & 產(chǎn)品資料

    • 參考文獻

    生物活性

    Diosgenin, a steroidal saponin, can inhibit STAT3 signaling pathway[1]. Diosgenin is an exogenous activator of Pdia3/ERp57[2]. Diosgenin inhibits aortic atherosclerosis progression by suppressing macrophage miR-19b expression[5].

    IC50 & Target[1]

    STAT3

     

    細胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    A549 IC50
    > 10 μM
    Compound: 1
    Cytotoxicity against human A549 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
    [PMID: 33381964]
    A549 IC50
    > 20 μM
    Compound: a1
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    [PMID: 30822712]
    A549 IC50
    10.8 μM
    Compound: Diosgenin
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Antiproliferative activity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    [PMID: 31546197]
    A549 IC50
    26.41 μM
    Compound: Diosgenin
    Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    [PMID: 31761382]
    A549 IC50
    32.85 μM
    Compound: DSG; 1
    Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    [PMID: 33740546]
    ASPC1 IC50
    63.11 μM
    Compound: Diosgenin
    Antiproliferative activity against human ASPC1 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay
    Antiproliferative activity against human ASPC1 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay
    [PMID: 35586422]
    BXPC-3 GI50
    1 μg/mL
    Compound: 7
    Growth inhibition of humanBxPC3 cells after 48 hrs
    Growth inhibition of humanBxPC3 cells after 48 hrs
    [PMID: 15921418]
    DU-145 GI50
    8.5 μg/mL
    Compound: 7
    Growth inhibition of human DU145 cells after 48 hrs
    Growth inhibition of human DU145 cells after 48 hrs
    [PMID: 15921418]
    GES1 IC50
    100.7 μM
    Compound: Diosgenin
    Cytotoxicity against human GES1 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human GES1 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    [PMID: 31761382]
    HBL-100 IC50
    10.8 μM
    Compound: Diosgenin
    Antiproliferative activity against human HBL100 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Antiproliferative activity against human HBL100 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    [PMID: 31546197]
    HCT-116 IC50
    10.8 μM
    Compound: Diosgenin
    Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Antiproliferative activity against human HCT116 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    [PMID: 31546197]
    HCT-116 IC50
    31.41 μM
    Compound: Diosgenin
    Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay
    Antiproliferative activity against human HCT-116 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay
    [PMID: 35586422]
    HCT-116 IC50
    49.11 μM
    Compound: Diosgenin
    Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    [PMID: 31761382]
    HeLa IC50
    > 50 μg/mL
    Compound: 21
    Cytotoxicity against human HeLa cells after 6 days by SRB assay
    Cytotoxicity against human HeLa cells after 6 days by SRB assay
    [PMID: 22880631]
    HepG2 IC50
    > 10 μM
    Compound: 1
    Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
    Cytotoxicity against human HepG2 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
    [PMID: 33381964]
    HepG2 IC50
    > 50 μM
    Compound: 1, Diosgenin
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay
    [PMID: 23153797]
    HepG2 IC50
    33.87 μM
    Compound: Diosgenin
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    [PMID: 31761382]
    HepG2 IC50
    48.36 μM
    Compound: DSG; 1
    Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    [PMID: 33740546]
    HL-60 IC50
    > 20 μM
    Compound: a1
    Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    Antiproliferative activity against human HL60 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    [PMID: 30822712]
    HT-29 IC50
    > 50 μg/mL
    Compound: 21
    Cytotoxicity against human HT-29 cells after 6 days by SRB assay
    Cytotoxicity against human HT-29 cells after 6 days by SRB assay
    [PMID: 22880631]
    HT-29 IC50
    10.8 μM
    Compound: Diosgenin
    Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    Antiproliferative activity against human HT-29 cells assessed as reduction in cell viability after 48 hrs by MTT assay
    [PMID: 31546197]
    K562 IC50
    > 50 μg/mL
    Compound: 21
    Cytotoxicity against human K562 cells after 6 days by SRB assay
    Cytotoxicity against human K562 cells after 6 days by SRB assay
    [PMID: 22880631]
    K562 IC50
    30.04 μM
    Compound: DSG; 1
    Cytotoxicity against human K562 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    Cytotoxicity against human K562 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    [PMID: 33740546]
    KM-20L2 GI50
    1.1 μg/mL
    Compound: 7
    Growth inhibition of human KM20L2 cells after 48 hrs
    Growth inhibition of human KM20L2 cells after 48 hrs
    [PMID: 15921418]
    MCF7 IC50
    > 10 μM
    Compound: 1
    Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
    Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay
    [PMID: 33381964]
    MCF7 IC50
    > 20 μM
    Compound: a1
    Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    [PMID: 30822712]
    MCF7 IC50
    > 50 μg/mL
    Compound: 21
    Cytotoxicity against human MCF7 cells after 6 days by SRB assay
    Cytotoxicity against human MCF7 cells after 6 days by SRB assay
    [PMID: 22880631]
    MCF7 GI50
    0.42 μg/mL
    Compound: 7
    Growth inhibition of human MCF7 cells after 48 hrs
    Growth inhibition of human MCF7 cells after 48 hrs
    [PMID: 15921418]
    MCF7 IC50
    23.91 μM
    Compound: Diosgenin
    Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay
    [PMID: 31761382]
    MCF7 IC50
    28.52 μM
    Compound: DSG; 1
    Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    [PMID: 33740546]
    MDA-MB-231 IC50
    > 50 μM
    Compound: 1, Diosgenin
    Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay
    Cytotoxicity against human MDA-MB-231 cells after 48 hrs by MTT assay
    [PMID: 23153797]
    MKN-45 IC50
    78.32 μM
    Compound: DSG; 1
    Cytotoxicity against human MNK45 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    Cytotoxicity against human MNK45 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    [PMID: 33740546]
    MM96L IC50
    > 50 μg/mL
    Compound: 21
    Cytotoxicity against human MM96L cells after 6 days by SRB assay
    Cytotoxicity against human MM96L cells after 6 days by SRB assay
    [PMID: 22880631]
    NCI-H358 IC50
    > 40 μM
    Compound: Diosgenin
    Antiproliferative activity against human NCI-H358 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay
    Antiproliferative activity against human NCI-H358 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay
    [PMID: 35586422]
    NCI-H460 IC50
    > 50 μM
    Compound: 1
    Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay
    Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay
    [PMID: 28606759]
    NCI-H460 GI50
    1.6 μg/mL
    Compound: 7
    Growth inhibition of human NCI-H460 cells after 48 hrs
    Growth inhibition of human NCI-H460 cells after 48 hrs
    [PMID: 15921418]
    NFF IC50
    > 50 μg/mL
    Compound: 21
    Cytotoxicity against human NFF cells after 6 days by SRB assay
    Cytotoxicity against human NFF cells after 6 days by SRB assay
    [PMID: 22880631]
    P388 ED50
    > 10 μg/mL
    Compound: 7
    Growth inhibition of mouse P388 cells after 48 hrs
    Growth inhibition of mouse P388 cells after 48 hrs
    [PMID: 15921418]
    SF-268 GI50
    1.6 μg/mL
    Compound: 7
    Growth inhibition of human SF268 cells after 48 hrs
    Growth inhibition of human SF268 cells after 48 hrs
    [PMID: 15921418]
    SMMC-7721 IC50
    > 20 μM
    Compound: a1
    Antiproliferative activity against human SMMC7721 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    Antiproliferative activity against human SMMC7721 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    [PMID: 30822712]
    SW480 IC50
    > 20 μM
    Compound: a1
    Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    Antiproliferative activity against human SW480 cells assessed as reduction in cell viability incubated for 48 hrs by MTS assay
    [PMID: 30822712]
    SW-620 IC50
    > 40 μM
    Compound: Diosgenin
    Antiproliferative activity against human SW-620 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay
    Antiproliferative activity against human SW-620 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK8 assay
    [PMID: 35586422]
    T-24 IC50
    28.85 μM
    Compound: DSG; 1
    Cytotoxicity against human T24 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    Cytotoxicity against human T24 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay
    [PMID: 33740546]
    THP-1 IC50
    6.96 μM
    Compound: 2
    Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
    Anticoagulant activity in human THP1 cells assessed as inhibition of TNF-alpha-stimulated tissue factor expression incubated 1 hr before TNFalpha challenge measured after 5 hrs
    [PMID: 20561785]
    體外研究
    (In Vitro)

    在 MC65 細胞 (一種細胞 AD 模型) 中,Diosgenin 在 1 μM 時表現(xiàn)出較弱的保護能力,可能是抗 AD 病理學的二價化合物中作為甾體部分的良好候選者[3]。Diosgenin 以劑量依賴性方式抑制 C3A 細胞中 STAT3 的組成型激活,最大抑制發(fā)生在 100 μM 左右[1]。
    Diosgenin (10 μM;24 h) 顯著降低泡沫細胞中 miR-19b 的水平[5]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    體內研究
    (In Vivo)

    Diosgenin 顯著降低 HF 飲食喂養(yǎng)小鼠的禁食和再進食血糖水平。 與 HF 飲食喂養(yǎng)的小鼠相比,Diosgenin 顯著降低 IPGTT 試驗中的血糖水平[4]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    414.62

    Formula

    C27H42O3

    CAS 號
    性狀

    固體

    顏色

    White to off-white

    中文名稱

    薯蕷皂素;薯蕷皂甙元;薯蕷皂苷元;地奧配質;薯芋皂苷配基;薯芋皂素

    結構分類
    初始來源
    運輸條件

    Room temperature in continental US; may vary elsewhere.

    儲存方式
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    溶解性數(shù)據(jù)
    細胞實驗: 

    Ethanol 中的溶解度 : 3.33 mg/mL (8.03 mM; 超聲助溶)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    DMSO 中的溶解度 : < 1 mg/mL (insoluble or slightly soluble)

    配制儲備液
    濃度 溶劑體積 質量 1 mg 5 mg 10 mg
    1 mM 2.4118 mL 12.0592 mL 24.1185 mL
    5 mM 0.4824 mL 2.4118 mL 4.8237 mL
    查看完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產(chǎn)品失效。
    儲備液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C儲存時,請在2年內使用, -20°C儲存時,請在1年內使用。

    • 摩爾計算器

    • 稀釋計算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動物實驗:

    請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

    以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液,再依次添加助溶劑:
    ——為保證實驗結果的可靠性,澄清的儲備液可以根據(jù)儲存條件,適當保存;體內實驗的工作液,建議您現(xiàn)用現(xiàn)配,當天使用
    以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比;如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶

    • 方案 一

      請依序添加每種溶劑: 10% EtOH    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 0.67 mg/mL (1.62 mM); 澄清溶液

      此方案可獲得 ≥ 0.67 mg/mL(飽和度未知)的澄清溶液。

      1 mL 工作液為例,取 100 μL 6.7 mg/mL 的澄清 EtOH 儲備液加到 400 μL PEG300 中,混合均勻;再向上述體系中加入 50 μL Tween-80,混合均勻;然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

      生理鹽水的配制:將 0.9 g 氯化鈉,溶解于 ddH?O 并定容至 100 mL,可以得到澄清透明的生理鹽水溶液。
    • 方案 二

      請依序添加每種溶劑: 10% EtOH    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.67 mg/mL (1.62 mM); 澄清溶液

      此方案可獲得 ≥ 0.67 mg/mL(飽和度未知)的澄清溶液。

      1 mL 工作液為例,取 100 μL 6.7 mg/mL 的澄清 EtOH 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中,混合均勻。

      2 g SBE-β-CD(磺丁基醚 β-環(huán)糊精)粉末定容于 10 mL 的生理鹽水中,完全溶解至澄清透明。

    以下溶解方案,請直接配制工作液。建議現(xiàn)用現(xiàn)配,在短期內盡快用完。 以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比; 如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象,可以通過加熱和/或超聲的方式助溶。

    • 方案 一

      請依序添加每種溶劑: Corn Oil

      Solubility: 2.5 mg/mL (6.03 mM); 懸濁液; 超聲助溶

    • 方案 二

      請依序添加每種溶劑: 15% Solutol HS 15    10% Cremophor EL    35% PEG400    40% Water

      Solubility: 10.31 mg/mL (24.87 mM); 懸濁液; 超聲助溶

    動物溶解方案計算器
    請輸入動物實驗的基本信息:

    給藥劑量

    mg/kg

    動物的平均體重

    g

    每只動物的給藥體積

    μL

    動物數(shù)量

    由于實驗過程有損耗,建議您多配一只動物的量
    計算結果
    工作液所需濃度 : mg/mL
    純度 & 產(chǎn)品資料

    純度: 99.35%

    參考文獻

    完整儲備液配制表

    * 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液;一旦配成溶液,請分裝保存,避免反復凍融造成的產(chǎn)品失效
    儲備液的保存方式和期限:-80°C, 2 years; -20°C, 1 year。-80°C儲存時,請在2年內使用, -20°C儲存時,請在1年內使用。

    可選溶劑 濃度 溶劑體積 質量 1 mg 5 mg 10 mg 25 mg
    Ethanol 1 mM 2.4118 mL 12.0592 mL 24.1185 mL 60.2962 mL
    5 mM 0.4824 mL 2.4118 mL 4.8237 mL 12.0592 mL
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱:
    Diosgenin
    目錄號:
    HY-N0177
    需求量: