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  2. Immunology/Inflammation
  3. COX
  4. Rutaecarpine

Rutaecarpine  (Synonyms: 吳茱萸次堿; Rutecarpine)

目錄號(hào): HY-N0147 純度: 99.09%
COA 產(chǎn)品使用指南

Rutaecarpine, 是吳茱萸中的生物堿，是COX-2的抑制劑，其IC50值為0.28 μM。

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Rutaecarpine Chemical Structure

Rutaecarpine Chemical Structure

CAS No. : 84-26-4

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥503
In-stock
1 mg ￥159
In-stock
5 mg ￥323
In-stock
10 mg ￥485
In-stock
25 mg ￥850
In-stock
50 mg ￥1200
In-stock
100 mg ￥1800
In-stock
200 mg   詢價(jià)  
500 mg   詢價(jià)  

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Customer Review

Other Forms of Rutaecarpine:

查看 COX 亞型特異性產(chǎn)品:

  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Rutaecarpine, an alkaloid of Evodia rutaecarpa, is an inhibitor of COX-2 with an IC50 value of 0.28 μM.

IC50 & Target[1]

COX-2

0.28 μM (IC50, in BMMC)

COX-1

8.7 μM (IC50, in BMMC)

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
CCD-18Co IC50
> 50 μM
Compound: 1
Antiproliferative activity against human CCD-18Co cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human CCD-18Co cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
CCD-841CoN IC50
> 50 μM
Compound: 1
Antiproliferative activity against human CCD-841CoN cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human CCD-841CoN cells assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
HCT-116 IC50
31.1 μM
Compound: 1
Antiproliferative activity against human HCT-116 cells harboring beta-catenin mutant assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human HCT-116 cells harboring beta-catenin mutant assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
HCT-15 IC50
> 50 μM
Compound: 1
Antiproliferative activity against human HCT-15 cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human HCT-15 cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
HEK293 EC50
2.06 μM
Compound: 2
Agonist activity at rat TRPV1 channel expressed in HEK293 cells assessed as induction of channel current at -60 mV holding potential by whole-cell patch-clamp method
Agonist activity at rat TRPV1 channel expressed in HEK293 cells assessed as induction of channel current at -60 mV holding potential by whole-cell patch-clamp method
[PMID: 27159637]
HeLa EC50
26.1 μM
Compound: 1a
Cytotoxicity against human HeLa cells by MTT assay
Cytotoxicity against human HeLa cells by MTT assay
[PMID: 28958621]
LS174T IC50
6.1 μM
Compound: 1
Antiproliferative activity against human LS174T cells harboring beta-catenin mutant assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human LS174T cells harboring beta-catenin mutant assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
RKO IC50
> 50 μM
Compound: 1
Antiproliferative activity against human RKO cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human RKO cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
SW480 IC50
> 50 μM
Compound: 1
Antiproliferative activity against human SW480 cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
Antiproliferative activity against human SW480 cells harboring wild type beta-catenin assessed as reduction in cell viability measured after 72 hrs by SRB assay
[PMID: 35544614]
體外研究
(In Vitro)

Rutaecarpine 具有多種有趣的生物學(xué)特性，如抗血栓形成、抗癌、抗炎和鎮(zhèn)痛、抗肥胖和體溫調(diào)節(jié)、血管舒張活性，以及對(duì)心血管和內(nèi)分泌系統(tǒng)的影響[2]。
Rutaecarpine 以濃度依賴性方式抑制 BMMC 中 PGD2 生成的 COX-2 和 COX-1 依賴階段，IC50 分別為 0.28 μM 和 8.7 μM。它以劑量依賴的方式抑制 COX-2 轉(zhuǎn)染的 HEK293 細(xì)胞依賴于 COX-2 的外源性花生四烯酸向 PGE2 的轉(zhuǎn)化[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

Rutaecarpine 在大鼠由λ-卡拉膠誘導(dǎo)的足跖水腫模型中通過腹腔注射顯示出體內(nèi)抗炎活性[1]。
Rutaecarpine以劑量依賴方式顯著減少了抗體形成細(xì)胞的數(shù)量并導(dǎo)致脾臟重量下降。此外，給予Rutaecarpine的小鼠表現(xiàn)出脾臟細(xì)胞數(shù)量減少，脾臟中總T細(xì)胞、CD4+細(xì)胞、CD8+細(xì)胞和B細(xì)胞數(shù)量均減少。IL-2、干擾素和IL-10 mRNA的表達(dá)在rutaecarpine處理后被顯著抑制。給予小鼠rutaecarpine后，CD4+IL-2+細(xì)胞的數(shù)量顯著減少[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

287.32

Formula

C18H13N3O
CAS 號(hào)
性狀

固體

顏色

Light yellow to yellow

中文名稱

吳茱萸次堿
結(jié)構(gòu)分類
初始來源
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 50 mg/mL (174.02 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.4804 mL 17.4022 mL 34.8044 mL
5 mM 0.6961 mL 3.4804 mL 6.9609 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料
參考文獻(xiàn)
Cell Assay
[1]

Rutaecarpine is dissolved in DMSO and diluted with appropriate medium before use. COX-1 and COX-2 cDNA-transfected HEK293 cells are prepared. For measuring inhibitory activity on COX-1 and COX-2 by rutaecarpine, cells in 1 mL of culture medium are seeded into each well of 24-well. After culture for 4 days, the supernatants are removed and 250 mL of fresh medium is added to the cells with or without rutaecarpine. After preincubation for 5 h at 37°C, the cells are further incubated at 37°C for 30 min with 50 mM arachidonic acid. All reactions are stopped by centrifugation at 120 g at 4°C for 5 min. Concentrations of PGE2 in the supernatant are measured[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1][3]

Rats: Rutaecarpine is dissolved in 0.1% carboxymethyl cellulose and diluted with appropriate medium before use. Male Splague-Dawley (SD) rats (180-220 g) are used in the study. Rutaecarpine administered intraperitoneally and, 1 h later, l-carrageenan solution is injected to right hind paw of rats. Paw volumes are measured using plethysmometer 5 h after l-carrageenan injection[1].

Mice: For the antibody response to SRBCs, rutaecarpine is administered at a single dose of 10 mg/kg, 20 mg/kg, 40 mg/kg or 80 mg/kg in 10 mL of 1% povidone solution intravenously. Control animals are given 1% povidone solution at 10 mL/kg. Specific pathogen-free female BALB/c mice are used in the study[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用， -20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?年內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4804 mL 17.4022 mL 34.8044 mL 87.0110 mL
5 mM 0.6961 mL 3.4804 mL 6.9609 mL 17.4022 mL
10 mM 0.3480 mL 1.7402 mL 3.4804 mL 8.7011 mL
15 mM 0.2320 mL 1.1601 mL 2.3203 mL 5.8007 mL
20 mM 0.1740 mL 0.8701 mL 1.7402 mL 4.3505 mL
25 mM 0.1392 mL 0.6961 mL 1.3922 mL 3.4804 mL
30 mM 0.1160 mL 0.5801 mL 1.1601 mL 2.9004 mL
40 mM 0.0870 mL 0.4351 mL 0.8701 mL 2.1753 mL
50 mM 0.0696 mL 0.3480 mL 0.6961 mL 1.7402 mL
60 mM 0.0580 mL 0.2900 mL 0.5801 mL 1.4502 mL
80 mM 0.0435 mL 0.2175 mL 0.4351 mL 1.0876 mL
100 mM 0.0348 mL 0.1740 mL 0.3480 mL 0.8701 mL
Help & FAQs
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產(chǎn)品名稱：
Rutaecarpine
目錄號(hào)：
HY-N0147
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