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  2. Epigenetics TGF-beta/Smad Apoptosis Anti-infection
  3. PKC Apoptosis Bacterial HSV
  4. Verbascoside

Verbascoside  (Synonyms: 麥角甾苷; Acteoside; Kusaginin; TJC160)

目錄號: HY-N0021 純度: 99.83%
COA 產(chǎn)品使用指南

Verbascoside 是從 Acanthus mollis 中得到的糖苷類物質(zhì)，為 ATP 競爭性的 PKC 抑制劑，IC50 值為 25 μM，具有抗腫瘤，抗炎，抗神經(jīng)性疼痛等作用。

MCE 的所有產(chǎn)品僅用作科學(xué)研究或藥證申報(bào)，我們不為任何個(gè)人用途提供產(chǎn)品和服務(wù)

Verbascoside Chemical Structure

Verbascoside Chemical Structure

CAS No. : 61276-17-3

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥550
In-stock
5 mg ￥400
In-stock
10 mg ￥650
In-stock
50 mg ￥2300
In-stock
100 mg ￥3695
In-stock
200 mg   詢價(jià)  
500 mg   詢價(jià)  

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Customer Review

Other Forms of Verbascoside:

查看 PKC 亞型特異性產(chǎn)品:

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  • 生物活性

  • 實(shí)驗(yàn)參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Verbascoside is isolated from Acanthus mollis, acts as an ATP-competitive inhibitor of PKC, with an IC50 of 25 μM, and has antitumor, anti-inflammatory and antineuropathic pain activity.

IC50 & Target[1]

PKC

25 μM (IC50)

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 IC50
5.81 μM
Compound: 2
Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
[PMID: 25981688]
Erythrocyte IC50
28 μM
Compound: 6
Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs
Antioxidant activity assessed as inhibition of AAPH-induced hemolysis in rat RBC after 3 hrs
[PMID: 12828473]
HCT-15 IC50
2.73 μM
Compound: 2
Cytotoxicity against human HCT15 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
Cytotoxicity against human HCT15 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
[PMID: 25981688]
HEp-2 EC50
0.8 μg/mL
Compound: 1, verbascoside
Antiviral activity against RSV long infected in human Hep2 cells assessed as virus-induced cytopathic effect after 3 days
Antiviral activity against RSV long infected in human Hep2 cells assessed as virus-induced cytopathic effect after 3 days
[PMID: 9599250]
HEp-2 IC50
44 μg/mL
Compound: 1, verbascoside
Cytotoxicity against mCMV RM-461 infected human Hep2 cells after 3 days
Cytotoxicity against mCMV RM-461 infected human Hep2 cells after 3 days
[PMID: 9599250]
HEp-2 IC50
71 μg/mL
Compound: 1, verbascoside
Cytotoxicity against HSV2 infected human Hep2 cells after 3 days
Cytotoxicity against HSV2 infected human Hep2 cells after 3 days
[PMID: 9599250]
HEp-2 EC50
76.9 μg/mL
Compound: 1, verbascoside
Cytotoxicity against RSV long infected human Hep2 cells after 3 days
Cytotoxicity against RSV long infected human Hep2 cells after 3 days
[PMID: 9599250]
HEp-2 EC50
9.7 μg/mL
Compound: 1, verbascoside
Antiviral activity against RSV A-2 infected in human Hep2 cells after 3 days by plaque neutralization assay
Antiviral activity against RSV A-2 infected in human Hep2 cells after 3 days by plaque neutralization assay
[PMID: 9599250]
Hepatocyte IC50
4.6 μM
Compound: 5
Hepatoprotective activity in ddY mouse primary cultured hepatocytes assessed as inhibition of D-galactosamine-induced cytotoxicity after 44 hrs by MTT assay
Hepatoprotective activity in ddY mouse primary cultured hepatocytes assessed as inhibition of D-galactosamine-induced cytotoxicity after 44 hrs by MTT assay
[PMID: 20159656]
J774.A1 EC50
4.9 x 10-4 M
Compound: 2
Cytotoxicity against mouse J774A1 macrophages grown in monolayers by MTT assay
Cytotoxicity against mouse J774A1 macrophages grown in monolayers by MTT assay
[PMID: 16309308]
L1210 IC50
13 μM
Compound: 1
Antiproliferative activity against mouse L1210 cells after 48 hrs by coulter counting analysis
Antiproliferative activity against mouse L1210 cells after 48 hrs by coulter counting analysis
[PMID: 1812212]
L929 IC50
17.8 μg/mL
Compound: 5
Cytoprotective activity in mouse L929 cells assessed as inhibition of TNF-alpha-induced cytotoxicity after 44 hrs by MTT assay
Cytoprotective activity in mouse L929 cells assessed as inhibition of TNF-alpha-induced cytotoxicity after 44 hrs by MTT assay
[PMID: 20159656]
SK-MEL-2 IC50
8.52 μM
Compound: 2
Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
[PMID: 25981688]
SK-OV-3 IC50
7.61 μM
Compound: 2
Cytotoxicity against human SKOV3 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
Cytotoxicity against human SKOV3 cells assessed as inhibition of cell growth incubated for 48 hrs by SRB assay
[PMID: 25981688]
體外研究
(In Vitro)

Verbascoside 是一種 ATP 競爭性 PKC 抑制劑，其 IC50 為 25 μM。Verbascoside 對 ATP 和組蛋白的 Ki 分別為 22 μM 和 28 μM。Verbascoside 對 L-1210 細(xì)胞具有較強(qiáng)的抗腫瘤活性，其 IC50 為 13 μM[1]。Verbascoside (5 μM 和 10 μM) 抑制 2,4-二硝基氯苯 (DNCB) 誘導(dǎo)的 THP-1 細(xì)胞 T 細(xì)胞共刺激因子 CD86 和 CD54、促炎細(xì)胞因子及 NF-κB 信號通路的激活[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

Verbascoside (1%) 可降低 2,4-二硝基氯苯 (DNCB) 誘發(fā)的特應(yīng)性皮炎 (AD) 小鼠模型中總體抓撓行為發(fā)生率以及皮膚病變嚴(yán)重程度。Verbascoside 還可阻斷 DNCB 誘導(dǎo)的皮膚病變中促炎細(xì)胞因子 TNF-α、IL-6 和 IL-4 mRNA 表達(dá)[2]。Verbascoside (50、100 mg/kg，腹腔注射) 不會改變慢性壓迫性損傷 (CCI) 誘發(fā)的冷異常性疼痛。Verbascoside (200 mg/kg，腹腔注射) 在第 3 天降低大鼠對冷刺激丙酮的超敏反應(yīng)。Verbascoside 還可顯著減少與神經(jīng)病變相關(guān)的行為變化。此外，Verbascoside 在第 3 天降低 Bax 并增加 Bcl-2[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

624.59

Formula

C29H36O15
CAS 號
性狀

固體

顏色

White to yellow

中文名稱

毛蕊花糖苷；毛蕊花甙；麥角甾苷；類葉升麻苷
結(jié)構(gòu)分類
初始來源
運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

H2O 中的溶解度 : ≥ 100 mg/mL (160.11 mM)

DMSO 中的溶解度 : ≥ 100 mg/mL (160.11 mM; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 1.6011 mL 8.0053 mL 16.0105 mL
5 mM 0.3202 mL 1.6011 mL 3.2021 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物實(shí)驗(yàn)：

請根據(jù)您的 實(shí)驗(yàn)動(dòng)物和給藥方式 選擇適當(dāng)?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (3.33 mM); 澄清溶液

    此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (3.33 mM); 澄清溶液

    此方案可獲得 ≥ 2.08 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 20.8 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動(dòng)物溶解方案計(jì)算器
請輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
該產(chǎn)品水溶性佳，請具體參考實(shí)測 水 / PBS / Saline 中的溶解度數(shù)據(jù)。
您所需的儲備液濃度超過該產(chǎn)品的實(shí)測溶解度，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
純度 & 產(chǎn)品資料

純度: 99.83%

參考文獻(xiàn)
Cell Assay
[1]

The lymphocytic mouse leukemia L1210 cells (ATCC, CCL 219) are plated sparsely at 104 cells per well in 24-well cluster plates in Dulbecco’s modified Eagle medium containing 10% fetal calf serum, 4 mM glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and Verbascoside (solubilized in DMSO). After a 2-day incubation period at 37°C in a humidified atmosphere (5% CO2 in air), growth is monitored by counting cell numbers in a Coulter-counter. IC50 values are calculated on the basis of the linear regression lines established for each compound tested[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[2]

Rats[2]
To induce atopic dermatitis (AD)-like symptoms, 2,4-dinitrochlorobenzene (DNCB) is used. Briefly, the dorsal hair of the mice is removed using an electronic clipper 2 days before DNCB treatment. An application of 200 μL of 1% DNCB (in acetone:olive oil = 4:1) is made to the shaved dorsal skin for sensitization. The repeated challenge is performed on the same site with 0.2% DNCB once every 3 days for about 2 weeks. The mice are divided into 4 groups (n = 6 per group): (1) vehicle-treated controls, (2) DNCB-treated only, (3) 1% Verbascoside (in acetone:olive oil 4:1)-treated only, and (4) DNCB + 1% Verbascoside-treated group[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻(xiàn)

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用，-20°C儲存時(shí)，請?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.6011 mL 8.0053 mL 16.0105 mL 40.0263 mL
5 mM 0.3202 mL 1.6011 mL 3.2021 mL 8.0053 mL
10 mM 0.1601 mL 0.8005 mL 1.6011 mL 4.0026 mL
15 mM 0.1067 mL 0.5337 mL 1.0674 mL 2.6684 mL
20 mM 0.0801 mL 0.4003 mL 0.8005 mL 2.0013 mL
25 mM 0.0640 mL 0.3202 mL 0.6404 mL 1.6011 mL
30 mM 0.0534 mL 0.2668 mL 0.5337 mL 1.3342 mL
40 mM 0.0400 mL 0.2001 mL 0.4003 mL 1.0007 mL
50 mM 0.0320 mL 0.1601 mL 0.3202 mL 0.8005 mL
60 mM 0.0267 mL 0.1334 mL 0.2668 mL 0.6671 mL
80 mM 0.0200 mL 0.1001 mL 0.2001 mL 0.5003 mL
100 mM 0.0160 mL 0.0801 mL 0.1601 mL 0.4003 mL

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
Verbascoside
目錄號：
HY-N0021
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