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  2. Immunology/Inflammation Apoptosis Metabolic Enzyme/Protease
  3. COX Ferroptosis Endogenous Metabolite
  4. (-)-Epicatechin

(-)-Epicatechin  (Synonyms: 表兒茶素; (-)-Epicatechol; Epicatechin; epi-Catechin)

目錄號: HY-N0001 純度: ≥98.0%
COA 產(chǎn)品使用指南 技術(shù)支持

(-)-Epicatechin 抑制環(huán)加氧酶-1 (COX-1) , IC50為 3.2 μM。(-)-Epicatechin 通過阻斷 NF-κB 的 p65 亞基的核定位來抑制 IL-1β 誘導的 iNOS 表達。

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(-)-Epicatechin Chemical Structure

(-)-Epicatechin Chemical Structure

CAS No. : 490-46-0

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規(guī)格 價格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ￥770
In-stock
1 mg ￥218
In-stock
5 mg ￥437
In-stock
10 mg ￥700
In-stock
50 mg ￥1100
In-stock
100 mg ￥1600
In-stock
200 mg   詢價  
500 mg   詢價  

* Please select Quantity before adding items.

Customer Review

Other Forms of (-)-Epicatechin:

查看 COX 亞型特異性產(chǎn)品:

  • 生物活性

  • 實驗參考方法

  • 純度 & 產(chǎn)品資料

  • 參考文獻

生物活性

(-)-Epicatechin inhibits cyclooxygenase-1 (COX-1) with an IC50 of 3.2 μM. (-)-Epicatechin inhibits the IL-1β-induced expression of iNOS by blocking the nuclear localization of the p65 subunit of NF-κB.

IC50 & Target[1]

COX-1

3.2 μM (IC50)

細胞效力
(Cellular Effect)
Cell Line Type Value Description References
A549 ED50
> 10 μg/mL
Compound: 47
Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
BMDC IC50
> 50 μM
Compound: 17
Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
[PMID: 25769817]
BMDC IC50
> 50 μM
Compound: 17
Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
[PMID: 25769817]
BMDC IC50
> 50 μM
Compound: 17
Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA
[PMID: 25769817]
BV-2 IC50
47.37 μM
Compound: 11
Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction
Antineuroinflammatory activity in human BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction
[PMID: 27623545]
HCT-8 ED50
> 10 μg/mL
Compound: 47
Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
KB ED50
> 10 μg/mL
Compound: 47
Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
L929 EC50
300 μM
Compound: epicatechin
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining
[PMID: 9287415]
L929 EC50
350 μM
Compound: epicatechin
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay
Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay
[PMID: 9287415]
LNCaP EC50
13 μM
Compound: 1
Decrease in androgen receptor protein expression in LNCaP cells by Western blotting
Decrease in androgen receptor protein expression in LNCaP cells by Western blotting
[PMID: 17383188]
MT4 CC50
136.2 μg/mL
Compound: 11
Cytotoxicity against human MT4 cells by MTT assay
Cytotoxicity against human MT4 cells by MTT assay
[PMID: 10425115]
MT4 IC50
34.1 μg/mL
Compound: 11
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay
[PMID: 10425115]
PC-3 IC50
> 500 μM
Compound: 2
In vitro inhibitory concentration against PC-3 cell line in MTT assay was determined after 3 days incubation
In vitro inhibitory concentration against PC-3 cell line in MTT assay was determined after 3 days incubation
[PMID: 15380225]
Platelet IC50
> 50 μg/mL
Compound: (-)-epi-Catechin
Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry
Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry
[PMID: 21106454]
Platelet IC50
22.22 μg/mL
Compound: (-)-epi-Catechin
Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry
Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry
[PMID: 21106454]
SK-OV-3 IC50
> 500 μM
Compound: 2
In vitro inhibitory concentration against SKOV3 cell line in MTT assay was determined after 3 days incubation
In vitro inhibitory concentration against SKOV3 cell line in MTT assay was determined after 3 days incubation
[PMID: 15380225]
TE-671 ED50
> 10 μg/mL
Compound: 47
Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay
[PMID: 1431932]
U-373MG ATCC IC50
> 500 μM
Compound: 2
In vitro inhibitory concentration against U373MG cell line in MTT assay was determined after 3 days incubation
In vitro inhibitory concentration against U373MG cell line in MTT assay was determined after 3 days incubation
[PMID: 15380225]
Vero IC50
184.1 μM
Compound: 5
Cytotoxicity against african green monkey Vero cells after 4 hrs by MTT assay
Cytotoxicity against african green monkey Vero cells after 4 hrs by MTT assay
[PMID: 16724851]
體外研究
(In Vitro)

(-)-Epicatechin 在 70 μg/mL 時對環(huán)氧合酶-1 (COX-1) 表現(xiàn)出 >95% 的抑制活性，IC50 為 3.2 μM[1]。
(-)-Epicatechin 通過阻斷 NF-κB 的 p65 亞基的核定位來抑制 IL-1β 誘導的 iNOS 表達。在 RINm5F 細胞中，(-)- Epicatechin 顯示可阻斷添加 IL-1β 后胰島素釋放的抑制。此外，(-)-Epicatechin 顯示可抑制霍奇金淋巴瘤細胞和 Jurkat T 細胞的增殖，這歸因于 (-)-Epicatechin 抑制這些細胞中 NF-κB 與 DNA 結(jié)合的能力。在人結(jié)直腸癌 HCT-116 細胞中，將 20 μM Panaxadiol 與 150、200 或 250 μM (-)-Epicatechin 結(jié)合使用可分別導致 51%、97% 和 95% 的生長抑制。如膜聯(lián)蛋白 V/PI 染色所檢測，該組合還將細胞凋亡水平提高了 11.9%、16.6% 和 25.8%[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
體內(nèi)研究
(In Vivo)

動物通過口服強飼法 (每天兩次) 接受 1 mg/kg 的 (-)-Epicatechin 或水 (載體)。運動組進行 15 天的跑步機運動。使用 (-)-Epicatechin 可顯著提高跑步機性能 (~50%) 和增強原位肌肉抗疲勞性 (~30%)[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量

290.27

Formula

C15H14O6
CAS 號
性狀

固體

顏色

White to light yellow

中文名稱

表兒茶素；(-)-表兒茶素；表兒茶精；表兒茶酚
結(jié)構(gòu)分類
初始來源
運輸條件

Room temperature in continental US; may vary elsewhere.
儲存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性數(shù)據(jù)
細胞實驗： 

DMSO 中的溶解度 : 100 mg/mL (344.51 mM; 超聲助溶; 吸濕的 DMSO 對產(chǎn)品的溶解度有顯著影響，請使用新開封的 DMSO)

H2O 中的溶解度 : 2 mg/mL (6.89 mM; 超聲助溶 (<60°C))

配制儲備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 3.4451 mL 17.2253 mL 34.4507 mL
5 mM 0.6890 mL 3.4451 mL 6.8901 mL
查看完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

  • 摩爾計算器

  • 稀釋計算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動物實驗：

請根據(jù)您的 實驗動物和給藥方式 選擇適當?shù)娜芙夥桨浮?

以下溶解方案都請先按照 In Vitro 方式配制澄清的儲備液，再依次添加助溶劑：
——為保證實驗結(jié)果的可靠性，澄清的儲備液可以根據(jù)儲存條件，適當保存；體內(nèi)實驗的工作液，建議您現(xiàn)用現(xiàn)配，當天使用；
以下溶劑前顯示的百分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 方案 一

    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.61 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 400 μL PEG300 中，混合均勻；再向上述體系中加入 50 μL Tween-80，混合均勻；然后再繼續(xù)加入 450 μL 生理鹽水 定容至 1 mL。

    生理鹽水的配制：將 0.9 g 氯化鈉，溶解于 ddH?O 并定容至 100 mL，可以得到澄清透明的生理鹽水溶液。
  • 方案 二

    請依序添加每種溶劑： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.61 mM); 澄清溶液

    此方案可獲得 ≥ 2.5 mg/mL（飽和度未知）的澄清溶液。

    以 1 mL 工作液為例，取 100 μL 25.0 mg/mL 的澄清 DMSO 儲備液加到 900 μL 20% 的 SBE-β-CD 生理鹽水水溶液 中，混合均勻。

    2 g SBE-β-CD（磺丁基醚 β-環(huán)糊精）粉末定容于 10 mL 的生理鹽水中，完全溶解至澄清透明。
動物溶解方案計算器
請輸入動物實驗的基本信息：

給藥劑量

mg/kg

動物的平均體重

g

每只動物的給藥體積

μL

動物數(shù)量

只
由于實驗過程有損耗，建議您多配一只動物的量
請輸入您的動物體內(nèi)配方組成：
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
如果您的動物是免疫缺陷鼠或者體弱鼠，建議 DMSO 中的在最后工作液體系中的占比盡量不超過 2%。
方案所需 助溶劑 包括：DMSO， ，均可在 MCE 網(wǎng)站選購。 ，Tween 80，均可在 MCE 網(wǎng)站選購。
計算結(jié)果
工作液所需濃度 : mg/mL
儲備液配制方法 : mg 藥物溶于 μL  DMSO（母液濃度為 mg/mL）。
您所需的儲備液濃度超過該產(chǎn)品的實測溶解度，以下方案僅供參考，如有需要，請與 MCE 中國技術(shù)支持聯(lián)系。
動物實驗體內(nèi)工作液的配制方法 : 取 μL DMSO 儲備液，加入 μL 。 μL ，混合均勻至澄清，再加 μL Tween 80，混合均勻至澄清，再加 μL 生理鹽水。
連續(xù)給藥周期超過半月以上，請謹慎選擇該方案。
請確保第一步儲備液溶解至澄清狀態(tài)，從左到右依次添加助溶劑。您可采用超聲加熱 （超聲清洗儀，建議頻次 20-40 kHz），渦旋吹打等方式輔助溶解。
純度 & 產(chǎn)品資料

純度: 99.00%

參考文獻
Animal Administration
[3]

Mice[3]
1-year-old, male C57BL/6N mice (n=25) are randomized into four groups. Mice in the (-)-Epicatechin groups 3 and 4 are given 1.0 mg/kg twice a day (morning and evening) for 15 consecutive days, whereas animals in the control groups 1 and 2 receive the vehicle (water). Both (-)-Epicatechin and vehicle are administered via oral gavage[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
參考文獻

完整儲備液配制表

* 請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C儲存時，請在6個月內(nèi)使用，-20°C儲存時，請在1個月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.4451 mL 17.2253 mL 34.4507 mL 86.1267 mL
5 mM 0.6890 mL 3.4451 mL 6.8901 mL 17.2253 mL
DMSO 10 mM 0.3445 mL 1.7225 mL 3.4451 mL 8.6127 mL
15 mM 0.2297 mL 1.1484 mL 2.2967 mL 5.7418 mL
20 mM 0.1723 mL 0.8613 mL 1.7225 mL 4.3063 mL
25 mM 0.1378 mL 0.6890 mL 1.3780 mL 3.4451 mL
30 mM 0.1148 mL 0.5742 mL 1.1484 mL 2.8709 mL
40 mM 0.0861 mL 0.4306 mL 0.8613 mL 2.1532 mL
50 mM 0.0689 mL 0.3445 mL 0.6890 mL 1.7225 mL
60 mM 0.0574 mL 0.2871 mL 0.5742 mL 1.4354 mL
80 mM 0.0431 mL 0.2153 mL 0.4306 mL 1.0766 mL
100 mM 0.0345 mL 0.1723 mL 0.3445 mL 0.8613 mL

* 備注：如您選擇水作為儲備液，請稀釋至工作液后，再用 0.22 μm 的濾膜過濾除菌后使用。

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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產(chǎn)品名稱：
(-)-Epicatechin
目錄號：
HY-N0001
需求量：