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  2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. Pyronin B

Pyronin B 是一種有機(jī)陽(yáng)離子染料，可用于細(xì)菌、霉菌和核糖核酸的染色。Pyronin B 也可作為小型疏水性 (SH) 蛋白通道抑制劑。

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Pyronin B Chemical Structure

Pyronin B Chemical Structure

CAS No. : 2150-48-3

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Customer Review

  • 生物活性

  • 純度 & 產(chǎn)品資料

  • 參考文獻(xiàn)

生物活性

Pyronin B is an organic cationic dye used for the staining of bacteria, mycobacteria and ribonucleic acids. Pyronin B is also used as a small hydrophobic (SH) protein channel inhibitor[1][2].

細(xì)胞效力
(Cellular Effect)
Cell Line Type Value Description References
HeLa IC50
2 μM
Compound: 2
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by flow cytometry
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by flow cytometry
[PMID: 35647549]
體外研究
(In Vitro)

Pyronin B (0-15 μM) inhibits channel activity of approximately 60% of small hydrophobic (SH) proteins at a concentration of 10 μM, and the Kd value of 6.8 μM[1].
Pyronin B (0-0.25 μM) affects RSV replication in Vero cells in a dose-dependent manner, and its TCID50 (50% tissue culture infection dose) = 0 at the concentration of 0.25 μM[1].
Pyronin B (10 μM) can react with bromate in an oxidation reaction and the reaction rate increases dramatically with the addition of trace amounts of Ti (IV) with the characteristic absorption spectra of 555 nm[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

358.90

Formula

C21H27ClN2O
CAS 號(hào)
性狀

固體

顏色

Brown to black

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.
儲(chǔ)存方式

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性數(shù)據(jù)
細(xì)胞實(shí)驗(yàn)： 

DMSO 中的溶解度 : 20.83 mg/mL (58.04 mM; 超聲助溶 (<60°C); 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響，請(qǐng)使用新開封的 DMSO)

配制儲(chǔ)備液
濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
1 mM 2.7863 mL 13.9315 mL 27.8629 mL
5 mM 0.5573 mL 2.7863 mL 5.5726 mL
查看完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

  • 摩爾計(jì)算器

  • 稀釋計(jì)算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質(zhì)量
=
濃度
×
體積
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

濃度 (start)

C1

×
體積 (start)

V1

=
濃度 (final)

C2

×
體積 (final)

V2

動(dòng)物溶解方案計(jì)算器
請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息：

給藥劑量

mg/kg

動(dòng)物的平均體重

g

每只動(dòng)物的給藥體積

μL

動(dòng)物數(shù)量

只
由于實(shí)驗(yàn)過程有損耗，建議您多配一只動(dòng)物的量
計(jì)算結(jié)果
工作液所需濃度 : mg/mL
純度 & 產(chǎn)品資料

參考文獻(xiàn)

完整儲(chǔ)備液配制表

* 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請(qǐng)分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用，-20°C儲(chǔ)存時(shí)，請(qǐng)?jiān)?個(gè)月內(nèi)使用。

可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7863 mL 13.9315 mL 27.8629 mL 69.6573 mL
5 mM 0.5573 mL 2.7863 mL 5.5726 mL 13.9315 mL
10 mM 0.2786 mL 1.3931 mL 2.7863 mL 6.9657 mL
15 mM 0.1858 mL 0.9288 mL 1.8575 mL 4.6438 mL
20 mM 0.1393 mL 0.6966 mL 1.3931 mL 3.4829 mL
25 mM 0.1115 mL 0.5573 mL 1.1145 mL 2.7863 mL
30 mM 0.0929 mL 0.4644 mL 0.9288 mL 2.3219 mL
40 mM 0.0697 mL 0.3483 mL 0.6966 mL 1.7414 mL
50 mM 0.0557 mL 0.2786 mL 0.5573 mL 1.3931 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

產(chǎn)品名稱：
Pyronin B
目錄號(hào)：
HY-D1543
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