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  3. TMRM Perchlorate

羅丹明類染料是一種膜滲透性的陽離子熒光探針,可對(duì)線粒體膜電位進(jìn)行特異性識(shí)別,從而附著在線粒體中,并產(chǎn)生明亮熒光,在一定濃度下,羅丹明類染料對(duì)細(xì)胞具有低毒性,因此普遍用于檢測(cè)動(dòng)物細(xì)胞、植物細(xì)胞以及微生物中的線粒體。

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TMRM Perchlorate Chemical Structure

TMRM Perchlorate Chemical Structure

CAS No. : 115532-50-8

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規(guī)格 價(jià)格 是否有貨 數(shù)量
10 mM * 1 mL in DMSO ¥551
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5 mg ¥500
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10 mg ¥800
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50 mg ¥2800
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100 mg ¥4500
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200 mg   詢價(jià)  
500 mg   詢價(jià)  

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Customer Review

Other Forms of TMRM Perchlorate:

    TMRM Perchlorate purchased from MCE. Usage Cited in: Endocrinology. 2020 Sep 1;161(9):bqaa119.  [Abstract]

    Mitochondrial membrane potential detected by TMRE. TMRM staining reveals a decreased signal in the Immp2l knockdown granulosa cells compared to control cells, suggesting mitochondrial membrane potential depolarization. Melatonin treatment prevented the membrane depolarization in Immp2l knockdown granulosa cells.
    • 生物活性

    • 實(shí)驗(yàn)參考方法

    • 純度 & 產(chǎn)品資料

    • 參考文獻(xiàn)

    生物活性

    Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms[1].

    體外研究
    (In Vitro)

    1.TMRM 工作液的配制
    1.1 儲(chǔ)備液的配制
    取 1mg TMRM 溶于 339 uL DMSO 中,得到 5 mM 儲(chǔ)備液。
    1.2 TMRM 工作液的配制
    用無血清細(xì)胞培養(yǎng)基或 PBS 稀釋儲(chǔ)備液,得到 1 -20 μM 工作液。
    注意:請(qǐng)根據(jù)實(shí)際情況調(diào)整 TMRM 工作液的濃度。
    2. 細(xì)胞染色
    2.1 懸浮細(xì)胞 (6 孔板)
    a.1000g 4℃ 離心 3-5 分鐘,棄上清。PBS 洗 2 次,每次 5 分鐘。細(xì)胞密度為 1×106/mL。
    b. 加入 1 mL 工作液,室溫孵育 5-30 分鐘。
    c. 400g 4℃ 離心 3-4 分鐘,棄上清。
    d. PBS 洗滌 2 次,每次 5 分鐘。
    e. 重懸細(xì)胞用無血清細(xì)胞培養(yǎng)基或 PBS。熒光顯微鏡或流式細(xì)胞儀觀察。
    2.2 貼壁細(xì)胞
    a. 在無菌蓋玻片上培養(yǎng)貼壁細(xì)胞。
    b. 從培養(yǎng)基中取出蓋玻片并吸出多余的培養(yǎng)基。
    c . 加入 100 μL 工作液,輕搖使之完全覆蓋細(xì)胞,然后室溫孵育 30-60 分鐘。
    d. 用培養(yǎng)基洗滌 2 次,每次 5 分鐘。熒光顯微鏡或流式細(xì)胞儀觀察。
    注:若采用流式細(xì)胞儀檢測(cè),細(xì)胞需重懸后再染色。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    500.93

    Formula

    C25H25ClN2O7

    CAS 號(hào)
    性狀

    固體

    顏色

    Green to dark green

    Emission (Em)

    576

    Excitation (Ex)

    550

    中文名稱

    四甲基羅丹明甲酯高氯酸鹽

    運(yùn)輸條件

    Room temperature in continental US; may vary elsewhere.

    儲(chǔ)存方式

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

    溶解性數(shù)據(jù)
    細(xì)胞實(shí)驗(yàn): 

    DMSO 中的溶解度 : 41.67 mg/mL (83.19 mM; 超聲助溶; 吸濕的 DMSO 對(duì)產(chǎn)品的溶解度有顯著影響,請(qǐng)使用新開封的 DMSO)

    配制儲(chǔ)備液
    濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg
    1 mM 1.9963 mL 9.9814 mL 19.9629 mL
    5 mM 0.3993 mL 1.9963 mL 3.9926 mL
    查看完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液;一旦配成溶液,請(qǐng)分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限:-80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)。-80°C儲(chǔ)存時(shí),請(qǐng)?jiān)?年內(nèi)使用, -20°C儲(chǔ)存時(shí),請(qǐng)?jiān)?年內(nèi)使用。

    • 摩爾計(jì)算器

    • 稀釋計(jì)算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質(zhì)量
    =
    濃度
    ×
    體積
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    濃度 (start)

    C1

    ×
    體積 (start)

    V1

    =
    濃度 (final)

    C2

    ×
    體積 (final)

    V2

    動(dòng)物溶解方案計(jì)算器
    請(qǐng)輸入動(dòng)物實(shí)驗(yàn)的基本信息:

    給藥劑量

    mg/kg

    動(dòng)物的平均體重

    g

    每只動(dòng)物的給藥體積

    μL

    動(dòng)物數(shù)量

    由于實(shí)驗(yàn)過程有損耗,建議您多配一只動(dòng)物的量
    計(jì)算結(jié)果
    工作液所需濃度 : mg/mL
    純度 & 產(chǎn)品資料

    純度: 99.69%

    染色示例
    參考文獻(xiàn)
    Cell Assay
    [1]

    Cultures are exposed to Millipore-filtered solutions (0.22 μm) containing TMRM Perchlorate for 1 hr at 37°C (except the experiment involving different durations of exposure to TMRM Perchlorate). After treatment, solutions are removed and growth media reapplied under sterile conditions, and cultures are post-incubated for 18 hours at 37°C (except for the experiment involving analysis at different time points after exposure). Cells are then stained with 2 mg/mL bisbenzimide for 20 min at room temperature. Coverslips are subsequently washed in saline and imaged using 2P microscopy. Apoptotic cells are identified as brightly fluorescent nuclei under UV excitation indicating DNA fragmentation. Cell survivability is calculated as the percentage of live, unstained cells (±SD) in five microscopic fields per treatment[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    參考文獻(xiàn)

    完整儲(chǔ)備液配制表

    * 請(qǐng)根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液;一旦配成溶液,請(qǐng)分裝保存,避免反復(fù)凍融造成的產(chǎn)品失效。
    儲(chǔ)備液的保存方式和期限:-80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)。-80°C儲(chǔ)存時(shí),請(qǐng)?jiān)?年內(nèi)使用, -20°C儲(chǔ)存時(shí),請(qǐng)?jiān)?年內(nèi)使用。

    可選溶劑 濃度 溶劑體積 質(zhì)量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.9963 mL 9.9814 mL 19.9629 mL 49.9072 mL
    5 mM 0.3993 mL 1.9963 mL 3.9926 mL 9.9814 mL
    10 mM 0.1996 mL 0.9981 mL 1.9963 mL 4.9907 mL
    15 mM 0.1331 mL 0.6654 mL 1.3309 mL 3.3271 mL
    20 mM 0.0998 mL 0.4991 mL 0.9981 mL 2.4954 mL
    25 mM 0.0799 mL 0.3993 mL 0.7985 mL 1.9963 mL
    30 mM 0.0665 mL 0.3327 mL 0.6654 mL 1.6636 mL
    40 mM 0.0499 mL 0.2495 mL 0.4991 mL 1.2477 mL
    50 mM 0.0399 mL 0.1996 mL 0.3993 mL 0.9981 mL
    60 mM 0.0333 mL 0.1664 mL 0.3327 mL 0.8318 mL
    80 mM 0.0250 mL 0.1248 mL 0.2495 mL 0.6238 mL
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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    產(chǎn)品名稱:
    TMRM Perchlorate
    目錄號(hào):
    HY-D0984A
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